FastQCFastQC Report
Mon 6 Jun 2016
ERR1211273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5707419
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200700.35164756608897996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172710.30260613422634647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111300.19500933784605617No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100450.054.428731
GTATCAA178050.038.9042051
ATCAACG224400.030.2985343
CAACGCA225400.030.1575055
TATCAAC232800.029.524672
TCAACGC234500.029.0335834
AACGCAG240250.028.6449226
GTACATG230200.025.4425981
GTGGTAT43250.025.3911251
ACGCAGA272050.024.848277
TACATGG235800.024.7319152
CGCAGAG280650.024.0701268
ACATGGG240000.023.3241213
TGGTATC46250.022.7230532
GCAGAGT307150.021.8552029
CATGGGG161100.021.7862344
TACCTGG36250.021.6141852
GAGTACT181850.020.8273512-13
ATGGGGG109900.020.7026045
CAGAGTA301850.019.9830710-11
Produced by FastQC (version 0.11.5)