##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211273_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5707419 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.030379756594 33.0 31.0 34.0 30.0 34.0 2 32.16717276232917 33.0 31.0 34.0 30.0 34.0 3 32.226622401474295 34.0 31.0 34.0 30.0 34.0 4 35.89067194821337 37.0 35.0 37.0 35.0 37.0 5 35.78807583603026 37.0 35.0 37.0 35.0 37.0 6 35.8054458591528 37.0 35.0 37.0 35.0 37.0 7 35.71785723108817 37.0 35.0 37.0 33.0 37.0 8 35.69924899503611 37.0 35.0 37.0 33.0 37.0 9 37.302363467619955 39.0 37.0 39.0 34.0 39.0 10-11 37.24555127282577 39.0 37.0 39.0 33.5 39.0 12-13 37.17049843370532 39.0 37.0 39.0 33.0 39.0 14-15 38.373678540159744 40.0 38.0 41.0 33.0 41.0 16-17 38.35015634913084 40.0 38.0 41.0 33.0 41.0 18-19 38.360815545520666 40.0 38.0 41.0 33.5 41.0 20-21 38.3294247364702 40.0 38.0 41.0 34.0 41.0 22-23 38.21651503069952 40.0 38.0 41.0 33.5 41.0 24-25 38.117943855182176 40.0 37.0 41.0 33.0 41.0 26-27 38.01069634102561 40.0 37.0 41.0 33.0 41.0 28-29 37.895629881037294 40.0 37.0 41.0 33.0 41.0 30-31 37.888453957909874 40.0 37.0 41.0 33.0 41.0 32-33 37.80507791700592 40.0 37.0 41.0 33.0 41.0 34-35 37.659286185226634 40.0 37.0 41.0 33.0 41.0 36-37 37.499370556113014 40.0 36.0 41.0 32.0 41.0 38-39 37.37619000812802 40.0 36.0 41.0 32.0 41.0 40-41 37.25446563499193 39.5 36.0 41.0 31.0 41.0 42-43 37.13253784241178 39.0 36.0 41.0 31.0 41.0 44-45 36.95756400572658 39.0 35.5 41.0 31.0 41.0 46-47 36.694742930210666 39.0 35.0 41.0 30.0 41.0 48-49 35.13720475051858 39.0 34.5 40.0 23.0 41.0 50-51 35.84824515249362 38.0 34.5 40.0 27.5 41.0 52-53 35.93203477789172 38.0 35.0 40.0 28.5 41.0 54-55 35.753359968840556 38.0 34.5 40.0 28.0 41.0 56-57 35.532834806766424 38.0 34.0 40.0 28.0 41.0 58-59 35.28880260937562 38.0 34.0 40.0 27.0 41.0 60-61 35.02616611817005 37.0 34.0 40.0 27.0 41.0 62-63 34.698293046296406 37.0 33.5 39.5 26.0 41.0 64-65 34.3567268322161 36.0 33.0 39.0 26.0 41.0 66-67 34.020980148820335 36.0 33.0 39.0 26.0 40.0 68-69 33.65830912361612 35.0 32.5 38.5 25.5 40.0 70-71 33.2959943014522 35.0 32.0 38.0 25.0 40.0 72-73 32.920006749110236 35.0 32.0 37.0 24.5 39.5 74-75 32.55641981077612 35.0 31.0 36.5 24.0 39.0 76-77 31.65447630531419 34.0 30.5 35.5 23.5 38.0 78-79 32.10873706661452 34.0 31.0 36.0 24.0 38.0 80-81 31.990659876206742 34.0 31.0 35.0 24.0 37.0 82-83 31.727229593621914 34.0 31.0 35.0 24.0 37.0 84-85 31.45055672274981 34.0 31.0 35.0 23.0 36.0 86-87 31.19088242864244 34.0 31.0 35.0 22.5 36.0 88-89 30.96434973496777 34.0 30.0 35.0 20.5 35.5 90-91 30.743074934572 34.0 30.0 35.0 20.0 35.0 92-93 30.549403066429853 34.0 30.0 35.0 20.0 35.0 94-95 30.34927687979453 34.0 29.0 35.0 19.5 35.0 96-97 30.153566787369215 34.0 29.0 35.0 19.0 35.0 98-99 29.929489669498594 33.5 29.0 35.0 18.5 35.0 100 27.4674916980863 31.0 24.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2919708849412679 1101 2 -0.27772614036335597 1101 3 -0.288995748299282 1101 4 -0.15016174881184696 1101 5 -0.1517783352384754 1101 6 -0.2786717047960181 1101 7 -0.1933138493689981 1101 8 -0.20131962624934374 1101 9 -0.33199718028455294 1101 10-11 -0.2931948565239608 1101 12-13 -0.4736723215375349 1101 14-15 -0.47915414889289565 1101 16-17 -0.5460515194166291 1101 18-19 -0.5536037571625272 1101 20-21 -0.5752092608425912 1101 22-23 -0.6440936390080694 1101 24-25 -0.6545748952582784 1101 26-27 -0.7019858077469578 1101 28-29 -0.7578963545806019 1101 30-31 -0.6711159242514455 1101 32-33 -0.5278530870034857 1101 34-35 -0.5529832908774566 1101 36-37 -0.6508355246264728 1101 38-39 -0.5511864678522116 1101 40-41 -0.5970663413631883 1101 42-43 -0.6305829488533803 1101 44-45 -0.6422628464922298 1101 46-47 -0.6129206395524136 1101 48-49 0.641797335126526 1101 50-51 -0.2693974458056587 1101 52-53 -0.5714495075940533 1101 54-55 -0.5808529190262774 1101 56-57 -0.6710515466436107 1101 58-59 -0.7406082354265138 1101 60-61 -0.6600755465908321 1101 62-63 -0.7050309318654797 1101 64-65 -0.7884338081125222 1101 66-67 -0.7414824503077782 1101 68-69 -0.7450652331225385 1101 70-71 -0.7524822253890662 1101 72-73 -0.8543042170790471 1101 74-75 -0.8860857789839613 1101 76-77 -0.7445484747805899 1101 78-79 -0.7756550513869946 1101 80-81 -0.5791695863057669 1101 82-83 -0.5418385333682245 1101 84-85 -0.6029869867902136 1101 86-87 -0.6225318625266922 1101 88-89 -0.5668954610217902 1101 90-91 -0.7108180431011846 1101 92-93 -0.6984826894796221 1101 94-95 -0.7723393562875209 1101 96-97 -0.7207591122658492 1101 98-99 -0.7687612785473092 1101 100 -0.7707323043069074 1102 1 -0.26973586889335266 1102 2 -0.2244935319862691 1102 3 -0.31902420188792036 1102 4 -0.19921501645072937 1102 5 -0.20771911898617645 1102 6 -0.2357502454389504 1102 7 -0.21052009284186113 1102 8 -0.28036558233284126 1102 9 -0.2919097896050644 1102 10-11 -0.36909854505092454 1102 12-13 -0.4563728760325958 1102 14-15 -0.5140430662220581 1102 16-17 -0.5061992313947101 1102 18-19 -0.5153285573320332 1102 20-21 -0.6441555849010854 1102 22-23 -0.6507430945852448 1102 24-25 -0.6369383487715155 1102 26-27 -0.5733010796181262 1102 28-29 -0.5971938768874168 1102 30-31 -0.7048376799372349 1102 32-33 -0.6391654667311073 1102 34-35 -0.5748162100439771 1102 36-37 -0.6237858435548986 1102 38-39 -0.5865004297524834 1102 40-41 -0.554645052712182 1102 42-43 -0.6407737723293891 1102 44-45 -0.6217294973320193 1102 46-47 -0.6053618600215245 1102 48-49 0.6564318520863566 1102 50-51 -0.3008485208285592 1102 52-53 -0.5431749766684177 1102 54-55 -0.6987404788265721 1102 56-57 -0.7666306075243909 1102 58-59 -0.9065188527251706 1102 60-61 -0.7088897870725717 1102 62-63 -0.6494951062106935 1102 64-65 -0.7033130553200451 1102 66-67 -0.6933845373295995 1102 68-69 -0.7774278360053231 1102 70-71 -0.8029225619758051 1102 72-73 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1104 50-51 -0.2623398695141361 1104 52-53 -0.6723193601662558 1104 54-55 -0.7157116858401835 1104 56-57 -0.7188915112988425 1104 58-59 -0.7271624034751412 1104 60-61 -0.7749363180268105 1104 62-63 -0.8494661916191006 1104 64-65 -0.8975305753896592 1104 66-67 -0.8288228287879136 1104 68-69 -0.8049935056745099 1104 70-71 -0.82720460523295 1104 72-73 -0.8720860076895534 1104 74-75 -0.7983149499347348 1104 76-77 -0.6511097254357523 1104 78-79 -0.5552121157878815 1104 80-81 -0.5252889267946479 1104 82-83 -0.728751577215089 1104 84-85 -0.6528786612262323 1104 86-87 -0.5845048936433663 1104 88-89 -0.6128396594924403 1104 90-91 -0.846580192746039 1104 92-93 -0.8105555241308551 1104 94-95 -0.8846603464166805 1104 96-97 -0.9506551512140966 1104 98-99 -0.9146980413454315 1104 100 -0.8619282588312736 1105 1 -0.3353465214781437 1105 2 -0.3290917331278891 1105 3 -0.4048286069064879 1105 4 -0.1723208489688446 1105 5 -0.18214417763427804 1105 6 -0.2510640958886654 1105 7 -0.45973131148767976 1105 8 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0.22798481911284085 2311 9 0.5135411490030961 2311 10-11 0.41315896047128575 2311 12-13 0.5039417538393351 2311 14-15 0.4487873696726936 2311 16-17 0.479807002070082 2311 18-19 0.6377870095838603 2311 20-21 0.7121025082671864 2311 22-23 0.7177468083918157 2311 24-25 0.675690704866831 2311 26-27 0.7163708568473979 2311 28-29 0.7228447617611735 2311 30-31 0.6270017565114259 2311 32-33 0.651844100612955 2311 34-35 0.7739410841587571 2311 36-37 0.735169164716929 2311 38-39 0.7914852017492748 2311 40-41 0.773338370516278 2311 42-43 0.8192893290800427 2311 44-45 0.8385712694908847 2311 46-47 0.9433958328972167 2311 48-49 -5.834891320736176 2311 50-51 -0.9961978665969227 2311 52-53 0.5061248166740029 2311 54-55 0.9076399588163966 2311 56-57 1.0235576661763588 2311 58-59 1.0682007425719462 2311 60-61 0.8832488213349379 2311 62-63 0.7107379095606703 2311 64-65 0.8786509952175194 2311 66-67 1.046157571068406 2311 68-69 0.9838031046025506 2311 70-71 1.035531389085179 2311 72-73 0.504130751471159 2311 74-75 0.7728646931187768 2311 76-77 0.8421122348301537 2311 78-79 0.8259048976375638 2311 80-81 0.8678613230012928 2311 82-83 0.8036358079140946 2311 84-85 0.8990005391216798 2311 86-87 0.9275996834654485 2311 88-89 0.9306078108706544 2311 90-91 0.9278549490175116 2311 92-93 0.9257624838552339 2311 94-95 0.9517226761120874 2311 96-97 0.9398875734891412 2311 98-99 1.0161525644234644 2311 100 1.2839183244320864 2312 1 0.12987266639433415 2312 2 0.2705131921313253 2312 3 0.23136755849260027 2312 4 0.06842319521098261 2312 5 0.24999575166209098 2312 6 0.15580612401684846 2312 7 0.3306564985767295 2312 8 0.38940125371539835 2312 9 0.33159561536546533 2312 10-11 0.2943684728488236 2312 12-13 0.324846792688291 2312 14-15 0.3907973336334152 2312 16-17 0.5094736892252243 2312 18-19 0.417304561055694 2312 20-21 0.4356599399208889 2312 22-23 0.6739943753849786 2312 24-25 0.7210828341440916 2312 26-27 0.6918256951303121 2312 28-29 0.8316767078913969 2312 30-31 0.8705119477231165 2312 32-33 0.8612759503474976 2312 34-35 0.40754626299963803 2312 36-37 0.7395931430251892 2312 38-39 0.8667783284161388 2312 40-41 0.6689587942113633 2312 42-43 0.7231438750479882 2312 44-45 0.8429643724700213 2312 46-47 0.8829342849940929 2312 48-49 -4.602205939104039 2312 50-51 -0.681991502552215 2312 52-53 0.6114149299657825 2312 54-55 0.902843586959925 2312 56-57 0.909499957690528 2312 58-59 0.9082650402665919 2312 60-61 1.012326616848803 2312 62-63 0.7412046813127944 2312 64-65 0.9966443217730188 2312 66-67 0.9669951286649194 2312 68-69 0.8505914733075102 2312 70-71 0.9084242191777463 2312 72-73 0.9838453512425147 2312 74-75 0.6861587945808623 2312 76-77 0.8616343441565775 2312 78-79 0.5507613630099186 2312 80-81 0.7357017584446837 2312 82-83 0.7686156025187607 2312 84-85 0.7756131214339064 2312 86-87 0.7862989655082764 2312 88-89 0.8476372091753781 2312 90-91 0.6542226179510777 2312 92-93 0.8566823813672038 2312 94-95 1.0270836381825852 2312 96-97 1.0519735035945281 2312 98-99 1.087494540281952 2312 100 0.7000099232383903 2313 1 0.14594989974273886 2313 2 0.25468501247042497 2313 3 0.32585685669343434 2313 4 0.10388341371270826 2313 5 0.14660742470259436 2313 6 0.27456624732294443 2313 7 0.25041622524611284 2313 8 0.3144948547698334 2313 9 0.535307897506847 2313 10-11 0.2830861507232143 2313 12-13 0.03644667058556905 2313 14-15 0.2051041432770262 2313 16-17 0.1881677739971579 2313 18-19 0.48773716916478094 2313 20-21 0.5293102043383229 2313 22-23 0.6262159610297644 2313 24-25 0.29804518735483043 2313 26-27 0.4599153986693665 2313 28-29 0.5407328378294167 2313 30-31 0.6186086687892285 2313 32-33 0.5308492680875858 2313 34-35 0.51180538612509 2313 36-37 -0.11518365882884041 2313 38-39 0.37856072913222505 2313 40-41 0.5374098942927361 2313 42-43 0.6241759518394119 2313 44-45 0.6658960711212956 2313 46-47 0.5077032245144295 2313 48-49 -5.186445198478964 2313 50-51 -1.2120091012823053 2313 52-53 0.26386145945556905 2313 54-55 0.43505600504477115 2313 56-57 0.44275582929606117 2313 58-59 0.5505930743626166 2313 60-61 0.911896239498553 2313 62-63 0.9463151301580837 2313 64-65 1.0278664387542378 2313 66-67 1.034393109229768 2313 68-69 1.0872959300520222 2313 70-71 1.0985091015931019 2313 72-73 0.8878543857923376 2313 74-75 0.9790601402686434 2313 76-77 0.9756133078144487 2313 78-79 0.9268696261057983 2313 80-81 -0.06765992425242473 2313 82-83 0.4136612387381682 2313 84-85 0.8029257080060503 2313 86-87 0.9355400170465948 2313 88-89 0.9166105898347787 2313 90-91 0.9229483318138527 2313 92-93 1.0464441487139347 2313 94-95 0.9587053299256958 2313 96-97 0.9705494215086112 2313 98-99 1.1330472177064834 2313 100 1.2171292490301369 2314 1 0.10518492606752261 2314 2 0.1999909582296837 2314 3 0.22146152156373233 2314 4 0.09236767372915011 2314 5 0.19257591718060496 2314 6 0.3192082013030628 2314 7 0.09528468570841397 2314 8 0.2010014284930861 2314 9 0.42657392567223695 2314 10-11 0.3334314034710033 2314 12-13 0.24103551366035703 2314 14-15 0.27164946526205824 2314 16-17 0.5105117373202788 2314 18-19 0.5291627418686815 2314 20-21 0.5700234116426444 2314 22-23 0.3380739514462263 2314 24-25 0.45979339806043384 2314 26-27 0.43994058041712236 2314 28-29 0.5274126572381519 2314 30-31 0.5194291489388121 2314 32-33 0.5290402050817775 2314 34-35 0.3311617095611936 2314 36-37 0.42492937720442114 2314 38-39 0.41940246603017073 2314 40-41 0.23623290159262922 2314 42-43 0.24049220482837086 2314 44-45 0.474617041900288 2314 46-47 0.5918098222202985 2314 48-49 -6.621996403295803 2314 50-51 -1.653529796656123 2314 52-53 -0.8823830036519951 2314 54-55 -0.3628073116308812 2314 56-57 -0.07074224630537174 2314 58-59 0.36754608737183503 2314 60-61 0.6298116133522811 2314 62-63 0.46812757604124045 2314 64-65 0.6515758135968852 2314 66-67 0.6734267210648284 2314 68-69 0.7723974159241322 2314 70-71 0.5644593952859083 2314 72-73 0.7056783393823807 2314 74-75 0.7533445145076882 2314 76-77 0.7087308514487631 2314 78-79 0.5162271334143469 2314 80-81 0.5606889021925099 2314 82-83 0.508685626600279 2314 84-85 0.5579086980298342 2314 86-87 0.6181286012670917 2314 88-89 0.5829995087168989 2314 90-91 0.7753940920111972 2314 92-93 0.7716409888246467 2314 94-95 0.8446933872646163 2314 96-97 0.8259773913971422 2314 98-99 0.918683012408458 2314 100 -0.4697164292026663 2315 1 0.18352529138621065 2315 2 0.24677054165577772 2315 3 0.2747865430350487 2315 4 0.13409312763570114 2315 5 0.22415161259644378 2315 6 0.29291732417897975 2315 7 0.3368813314489856 2315 8 0.25011472646348665 2315 9 0.4724001604824011 2315 10-11 0.24616575437818966 2315 12-13 0.4451009313373433 2315 14-15 0.27001349994080215 2315 16-17 0.4344063419404307 2315 18-19 0.48494596309948435 2315 20-21 0.382613585352118 2315 22-23 0.472882773874872 2315 24-25 0.4994441640789873 2315 26-27 0.5060214054936765 2315 28-29 0.667115644677466 2315 30-31 0.6249516879151216 2315 32-33 0.6635263939109919 2315 34-35 0.5968082510888806 2315 36-37 0.5080546402441399 2315 38-39 0.5724596375580759 2315 40-41 0.6731555418407353 2315 42-43 0.6998540694033366 2315 44-45 0.678912414717999 2315 46-47 0.5566698275979292 2315 48-49 -4.7536465261714085 2315 50-51 -0.976828219497385 2315 52-53 0.11613751352358292 2315 54-55 0.09902736182450411 2315 56-57 0.47890137961756807 2315 58-59 0.5573391538055148 2315 60-61 0.5521703659720529 2315 62-63 0.24514929245243877 2315 64-65 0.4538355583798577 2315 66-67 -0.2422379291153831 2315 68-69 0.4449517853521243 2315 70-71 0.4399189387516458 2315 72-73 0.5871134972885628 2315 74-75 0.7275289210766971 2315 76-77 0.7207691525299822 2315 78-79 0.591778363293848 2315 80-81 0.43394835058836634 2315 82-83 0.4516651939346552 2315 84-85 0.5216930792305305 2315 86-87 0.5234515990543294 2315 88-89 0.45962182520796446 2315 90-91 0.5747489627788411 2315 92-93 0.6198338048688115 2315 94-95 0.299201217565102 2315 96-97 0.3780714121167357 2315 98-99 0.3573464068305938 2315 100 0.42169211999971523 2316 1 0.19478073678716612 2316 2 0.23795013042402502 2316 3 0.1475637681654618 2316 4 -0.0025169674614176074 2316 5 0.15818986562494075 2316 6 0.1721347977260237 2316 7 0.24549590824013023 2316 8 0.2958581003528806 2316 9 0.42951781363173325 2316 10-11 0.43767703971456484 2316 12-13 0.34480636665077924 2316 14-15 0.4643965542110209 2316 16-17 0.46459165914212974 2316 18-19 0.17154177914886048 2316 20-21 0.5041481431138877 2316 22-23 0.6248146806947616 2316 24-25 0.6797418974053926 2316 26-27 0.7131926749491342 2316 28-29 0.7531423090864351 2316 30-31 0.6421994447059873 2316 32-33 0.5395008893197826 2316 34-35 0.5144420299382872 2316 36-37 0.3743877148001076 2316 38-39 0.5100196209033143 2316 40-41 0.1175972764533526 2316 42-43 0.10166287739343005 2316 44-45 0.402379445989439 2316 46-47 0.38325202599340713 2316 48-49 -4.8568216506080475 2316 50-51 -0.959299792332466 2316 52-53 0.29398541517220167 2316 54-55 0.5631297459398965 2316 56-57 0.47619330401207804 2316 58-59 0.3339646562878045 2316 60-61 0.522125208579503 2316 62-63 0.4897824827117603 2316 64-65 0.44078866082221424 2316 66-67 0.5058687186993325 2316 68-69 0.2767743844010013 2316 70-71 -0.20586735448932814 2316 72-73 -0.08414318847244573 2316 74-75 0.20074254775379785 2316 76-77 0.22003834194460126 2316 78-79 0.30371044066873054 2316 80-81 0.19453976601214862 2316 82-83 0.09270742784372032 2316 84-85 0.216469615695857 2316 86-87 0.2883066809144772 2316 88-89 0.2129043017330332 2316 90-91 0.21691910663714964 2316 92-93 0.35068046328555624 2316 94-95 0.566458888686725 2316 96-97 0.67037944573676 2316 98-99 0.5670924537419388 2316 100 0.09224413744052029 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 3.0 11 11.0 12 36.0 13 166.0 14 592.0 15 1706.0 16 4220.0 17 8576.0 18 14388.0 19 20961.0 20 29040.0 21 39559.0 22 48103.0 23 51023.0 24 52396.0 25 57422.0 26 67240.0 27 81336.0 28 100078.0 29 122235.0 30 150351.0 31 184424.0 32 232523.0 33 302606.0 34 415929.0 35 580482.0 36 878916.0 37 1236591.0 38 918974.0 39 107521.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.20876286446431 19.722149829198226 14.323530860255213 23.74555644608225 2 15.709496869980125 20.284154782496557 39.95167952969804 24.054668817825274 3 18.826628372629575 25.43259506578809 29.99296003655595 25.747816525026384 4 13.067522152538164 16.712888069200144 37.221013309836216 32.99857646842548 5 13.548797769629282 37.36177574626029 34.296698036511245 14.792728447599185 6 31.344581971143864 36.49342601040016 17.619984662063416 14.542007356392567 7 27.698702338132176 32.25778587484115 21.837524106781018 18.20598768024566 8 26.187417812077562 35.38056786214594 19.80016452916012 18.631849796616375 9 26.60246205487116 15.897449017051235 20.456839743193235 37.04324918488437 10-11 25.4930431069631 26.029113981050084 27.535869905548292 20.941973006438527 12-13 25.696368416271227 23.713359825777474 27.946371854781503 22.6438999031698 14-15 23.1288515168131 25.09714344184635 25.793764089686555 25.980240951653993 16-17 22.43781487663196 27.900632057292697 26.658401364966217 23.00315170110912 18-19 22.192167773208872 26.939287268027805 28.892376746827242 21.976168211936077 20-21 23.055106651181546 26.16389610391886 28.79664688800857 21.98435035689102 22-23 22.979862909640083 25.95018032280353 28.643839532284687 22.426117235271693 24-25 22.63100241195049 26.063704157434525 28.890124318999817 22.415169111615175 26-27 22.491828612427867 26.23556373594701 28.676134467179974 22.59647318444515 28-29 22.332468661107182 26.44626723540548 28.80404529936319 22.417218804124143 30-31 22.866705108911226 26.2012807189716 28.794169814848203 22.137844357268975 32-33 22.79032969574735 26.39904395636649 28.476379930968953 22.334246416917207 34-35 22.85944380500019 26.44305180339636 28.606836531411357 22.090667860192095 36-37 22.81650401428274 26.287385404002233 28.482414922397837 22.41369565931719 38-39 22.76676979069651 26.387913712989942 28.535389049400433 22.309927446913118 40-41 22.7257399632409 26.120345177026362 28.757506909962288 22.39640794977045 42-43 22.57828619528885 26.284156782741842 28.39758683910739 22.739970182861917 44-45 22.470310532537248 26.60697407667132 28.35530320632581 22.56741218446563 46-47 22.47719411623181 26.40182653842791 28.061220824248334 23.05975852109195 48-49 22.816760479289886 26.540650318911492 27.852537321320845 22.79005188047778 50-51 22.69462811890627 26.601398160232474 27.763815451961843 22.940158268899413 52-53 22.56831577849802 26.84100585219591 27.571982710655835 23.018695658650238 54-55 22.521972455185093 26.66606564173517 27.71722845851298 23.094733444566753 56-57 22.747215863826693 26.634699028205873 27.4573523159147 23.160732792052737 58-59 22.414855174836724 26.662163399388554 27.823130235102965 23.099851190671753 60-61 22.676549148294345 26.8197481188463 27.592832902527643 22.910869830331713 62-63 22.703552343683334 26.720008564888758 27.524105833849216 23.05233325757869 64-65 22.38710161881669 26.58888736330054 27.575611035372027 23.44839998251074 66-67 22.465952499293707 26.493869802441424 27.541198054208206 23.498979644056664 68-69 22.293549883868728 26.542556710661163 27.61080667089918 23.553086734570925 70-71 22.3367417246491 26.339061051269752 27.689550928839136 23.634646295242007 72-73 22.3975871837538 26.677339277749883 27.33721858436314 23.587854954133185 74-75 22.399043756649277 26.798013743150744 27.26046145242557 23.542481047774405 76-77 22.291215948865165 26.64869402965459 27.27781226003822 23.782277761442025 78-79 22.239326032391386 26.593528749572563 27.32763849464704 23.839506723389015 80-81 22.15247289703726 26.837099776451534 27.187144942580627 23.823282383930582 82-83 22.370864530475615 26.571856267750665 27.46831877357172 23.588960428201997 84-85 22.394235179132888 26.72295061080835 27.07211038240123 23.810703827657534 86-87 22.024553918798993 26.860041541246126 27.32847587228906 23.786928667665823 88-89 22.125916650476498 26.495290129115602 27.30767285994248 24.071120360465425 90-91 22.27837253847057 26.40760841945145 27.349591942954376 23.964427099123604 92-93 22.162422310835165 26.50060910688389 27.300736535688824 24.036232046592122 94-95 21.817131287538402 26.348088271175808 27.477353757114525 24.357426684171262 96-97 21.85933834955871 26.337110600217656 27.419153621916685 24.38439742830695 98-99 21.69040503536835 26.41536496712354 27.30582103189443 24.588408965613684 100 21.72257250459521 26.385368099713308 27.26863704664198 24.623422349049505 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2060.0 1 1517.5 2 2007.0 3 2698.5 4 1806.0 5 1320.5 6 1903.5 7 3691.5 8 5593.0 9 6445.0 10 5623.5 11 4406.5 12 4446.0 13 4965.0 14 5598.0 15 6320.0 16 7019.5 17 7635.0 18 8271.0 19 8905.0 20 9541.5 21 10516.5 22 12036.0 23 13933.0 24 16277.0 25 19321.0 26 23872.5 27 29445.0 28 35327.0 29 43117.5 30 53465.0 31 63856.5 32 75985.5 33 90308.5 34 103512.5 35 117025.5 36 133231.5 37 149874.5 38 158653.5 39 160740.0 40 159911.0 41 158969.5 42 163147.0 43 171890.0 44 182521.5 45 196891.5 46 209621.5 47 217317.5 48 225857.0 49 231958.0 50 232316.5 51 229954.5 52 225393.0 53 218761.5 54 210053.0 55 195138.5 56 177069.5 57 157424.5 58 138765.0 59 121663.5 60 101718.0 61 82471.5 62 66466.0 63 52719.5 64 39878.0 65 29256.0 66 21956.5 67 17223.0 68 13702.5 69 10664.5 70 8606.5 71 6720.5 72 5138.0 73 4085.0 74 3232.0 75 2507.0 76 1876.5 77 1221.5 78 743.5 79 482.5 80 309.5 81 202.0 82 131.5 83 83.0 84 53.5 85 33.0 86 26.5 87 15.5 88 6.0 89 6.5 90 5.0 91 3.0 92 3.0 93 2.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029470413859574704 2 0.0018747528436233612 3 5.2563163839907324E-5 4 0.0208150128806033 5 4.730684745591659E-4 6 3.504210922660488E-5 7 0.0 8 0.003924716233379747 9 0.009811790583449366 10-11 0.0010162211675715416 12-13 9.636580037316342E-5 14-15 1.4892896421307074E-4 16-17 4.38026365332561E-4 18-19 0.0 20-21 7.884474575986098E-5 22-23 2.7157634650618783E-4 24-25 8.76052730665122E-5 26-27 7.008421845320976E-5 28-29 9.461369491183318E-4 30-31 0.002733284519675181 32-33 0.009925677438435834 34-35 0.018835133709300123 36-37 0.021577178756281956 38-39 0.020736168134843438 40-41 0.0045642347267652855 42-43 0.0052300348020707784 44-45 0.00898830101662415 46-47 0.01170406448168603 48-49 1.205194502103315 50-51 0.017372125649089368 52-53 0.017766349377888676 54-55 0.008778048361264522 56-57 0.00696461920878772 58-59 0.00791075615790605 60-61 0.00495845845556459 62-63 0.006973379736094371 64-65 0.018563557362793935 66-67 0.02745549257904492 68-69 0.027884758417070832 70-71 0.025449331825821795 72-73 0.021445770846682186 74-75 0.00882185099779778 76-77 0.013683943652989205 78-79 0.007682982447933119 80-81 0.0027245239923685293 82-83 0.013429888361096322 84-85 0.022637202560386753 86-87 0.02247951306886703 88-89 0.031459053558184534 90-91 0.019430849566152406 92-93 0.012317301393151615 94-95 0.006071045423509295 96-97 0.003609337250340303 98-99 0.015856554425038707 100 0.035427572428097534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5707419.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.003823308231006 #Duplication Level Percentage of deduplicated Percentage of total 1 73.82174074700552 43.55764947342352 2 15.492552430504 18.28239652405926 3 5.121353762667154 9.065383575340705 4 2.1497861110590435 5.073823993896676 5 1.0640992512895746 3.1392962102755475 6 0.6106271993756527 2.161760362749657 7 0.39545615681154694 1.6333397641862428 8 0.264421114096203 1.2481485356078366 9 0.17904507330497127 0.9507909482546213 >10 0.8302616316456386 8.39943822555647 >50 0.04508292840843204 1.821979526726749 >100 0.023912112787332632 2.606550061603656 >500 0.0010573064609168679 0.40764215521337077 >1k 4.908918494314123E-4 0.5718482715245151 >5k 0.0 0.0 >10k+ 1.1328273448367478E-4 1.0799523715811554 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20070 0.35164756608897996 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17271 0.30260613422634647 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11130 0.19500933784605617 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.752105461330244E-5 2 0.0 0.0 0.0 0.0 1.752105461330244E-5 3 0.0 0.0 0.0 0.0 1.752105461330244E-5 4 0.0 0.0 0.0 0.0 3.504210922660488E-5 5 0.0 0.0 0.0 0.0 5.2563163839907324E-5 6 0.0 0.0 0.0 0.0 5.2563163839907324E-5 7 0.0 0.0 0.0 0.0 7.008421845320976E-5 8 0.0 0.0 0.0 0.0 8.76052730665122E-5 9 0.0 0.0 0.0 0.0 8.76052730665122E-5 10-11 0.0 0.0 0.0 0.0 8.76052730665122E-5 12-13 0.0 0.0 0.0 0.0 8.76052730665122E-5 14-15 0.0 0.0 0.0 0.0 1.0512632767981465E-4 16-17 0.0 0.0 0.0 1.752105461330244E-5 1.4016843690641952E-4 18-19 0.0 0.0 0.0 1.752105461330244E-5 1.5768949151972195E-4 20-21 0.0 0.0 0.0 2.628158191995366E-5 1.5768949151972195E-4 22-23 0.0 0.0 0.0 2.3653423727958294E-4 1.752105461330244E-4 24-25 0.0 0.0 0.0 7.97207984905261E-4 2.102526553596293E-4 26-27 0.0 0.0 0.0 0.0016645001882637318 2.628158191995366E-4 28-29 0.0 0.0 0.0 0.004651839999831798 2.628158191995366E-4 30-31 0.0 0.0 0.0 0.015269599095493077 3.153789830394439E-4 32-33 0.0 0.0 0.0 0.041752673143499716 3.241395103460951E-4 34-35 0.0 0.0 0.0 0.0826292935563343 3.7670267418600246E-4 36-37 0.0 0.0 0.0 0.14752727984400654 3.854632014926537E-4 38-39 0.0 1.752105461330244E-5 0.0 0.24053779825872257 4.029842561059561E-4 40-41 0.0 1.752105461330244E-5 0.0 0.3432725019838214 4.38026365332561E-4 42-43 0.0 1.752105461330244E-5 0.0 0.4456217425074276 4.38026365332561E-4 44-45 0.0 1.752105461330244E-5 0.0 0.5689349248758502 4.7306847455916594E-4 46-47 0.0 1.752105461330244E-5 0.0 0.7147626624223664 4.905895291724684E-4 48-49 0.0 1.752105461330244E-5 0.0 0.8721805775955822 4.993500564791195E-4 50-51 0.0 1.752105461330244E-5 0.0 1.0400673229002462 5.081105837857708E-4 52-53 0.0 1.752105461330244E-5 0.0 1.205667570577874 5.168711110924221E-4 54-55 0.0 1.752105461330244E-5 0.0 1.3978647791584953 5.256316383990732E-4 56-57 0.0 3.504210922660488E-5 0.0 1.6217663360618872 5.256316383990732E-4 58-59 0.0 3.504210922660488E-5 0.0 1.8710208589907276 5.256316383990732E-4 60-61 0.0 3.504210922660488E-5 0.0 2.122264021618178 5.256316383990732E-4 62-63 0.0 3.504210922660488E-5 0.0 2.375846245036504 5.256316383990732E-4 64-65 0.0 3.504210922660488E-5 0.0 2.635359345441433 5.256316383990732E-4 66-67 0.0 3.504210922660488E-5 0.0 2.928889573378089 5.256316383990732E-4 68-69 0.0 3.504210922660488E-5 0.0 3.251531033554747 5.256316383990732E-4 70-71 0.0 3.504210922660488E-5 0.0 3.591308085143215 5.431526930123757E-4 72-73 0.0 3.504210922660488E-5 0.0 3.918478737937411 5.781948022389805E-4 74-75 0.0 3.504210922660488E-5 0.0 4.248111098904776 5.781948022389805E-4 76-77 0.0 3.504210922660488E-5 0.0 4.614344942959331 6.044763841589341E-4 78-79 0.0 3.504210922660488E-5 0.0 5.010977816767965 6.132369114655854E-4 80-81 0.0 3.504210922660488E-5 0.0 5.4234322028924105 6.395184933855391E-4 82-83 0.0 3.504210922660488E-5 0.0 5.845339197980733 6.833211299187952E-4 84-85 0.0 3.504210922660488E-5 0.0 6.264863329641647 6.833211299187952E-4 86-87 0.0 3.504210922660488E-5 0.0 6.699403005106161 6.920816572254464E-4 88 0.0 3.504210922660488E-5 0.0 7.0459519443026695 7.008421845320976E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10045 0.0 54.42873 1 GTATCAA 17805 0.0 38.904205 1 ATCAACG 22440 0.0 30.298534 3 CAACGCA 22540 0.0 30.157505 5 TATCAAC 23280 0.0 29.52467 2 TCAACGC 23450 0.0 29.033583 4 AACGCAG 24025 0.0 28.644922 6 GTACATG 23020 0.0 25.442598 1 GTGGTAT 4325 0.0 25.391125 1 ACGCAGA 27205 0.0 24.84827 7 TACATGG 23580 0.0 24.731915 2 CGCAGAG 28065 0.0 24.070126 8 ACATGGG 24000 0.0 23.324121 3 TGGTATC 4625 0.0 22.723053 2 GCAGAGT 30715 0.0 21.855202 9 CATGGGG 16110 0.0 21.786234 4 TACCTGG 3625 0.0 21.614185 2 GAGTACT 18185 0.0 20.82735 12-13 ATGGGGG 10990 0.0 20.702604 5 CAGAGTA 30185 0.0 19.98307 10-11 >>END_MODULE