FastQCFastQC Report
Mon 6 Jun 2016
ERR1211272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9697805
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT562780.5803168861407298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT443030.456835335418685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290670.2997276187755889No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97120.10014637332880996No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA217000.055.5364651
GTATCAA421050.043.726631
ATCAACG513050.034.99643
CAACGCA517600.034.6786085
TATCAAC537450.033.9050752
AACGCAG556050.032.6360636
GTACTAG60400.032.31991
TCAACGC566900.031.8042134
GCATCGT54700.030.667478
ACGCAGA613200.029.1281537
CGCAGAG615000.029.0262748
GTGGTAT87200.028.482381
GCAGAGT656800.026.7230439
CTGTGCG65100.026.7084799
TAGGCAT80200.026.6581275
GTACATG551350.026.1238171
TGGTATC95250.025.5804352
TAACGCC33300.025.540754
TACATGG561450.025.259192
TCTAACG34600.024.7422082
Produced by FastQC (version 0.11.5)