##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211272_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9697805 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6978877178908 31.0 31.0 34.0 30.0 34.0 2 31.72984969279131 33.0 31.0 34.0 30.0 34.0 3 31.77647395467325 33.0 31.0 34.0 30.0 34.0 4 35.39808894899413 37.0 35.0 37.0 33.0 37.0 5 35.459581523860294 37.0 35.0 37.0 33.0 37.0 6 35.527079890758785 37.0 35.0 37.0 33.0 37.0 7 35.47329132726426 37.0 35.0 37.0 33.0 37.0 8 35.483616859691445 37.0 35.0 37.0 33.0 37.0 9 37.08477763782629 39.0 37.0 39.0 33.0 39.0 10-11 37.02998003156384 39.0 37.0 39.0 33.0 39.0 12-13 36.95223831578383 39.0 37.0 39.0 33.0 39.0 14-15 38.101559528161275 40.0 38.0 41.0 33.0 41.0 16-17 38.11912912251793 40.0 38.0 41.0 33.0 41.0 18-19 38.172924182327854 40.0 38.0 41.0 33.0 41.0 20-21 38.16029498427737 40.0 38.0 41.0 33.0 41.0 22-23 38.036874426738834 40.0 38.0 41.0 33.0 41.0 24-25 37.930886834701255 40.0 38.0 41.0 33.0 41.0 26-27 37.78715307226739 40.0 37.0 41.0 32.5 41.0 28-29 37.670607524073745 40.0 37.0 41.0 32.0 41.0 30-31 37.478562829423765 40.0 37.0 41.0 32.0 41.0 32-33 37.3374968871822 40.0 36.0 41.0 32.0 41.0 34-35 37.19389073094376 40.0 36.0 41.0 31.0 41.0 36-37 37.09751737635476 39.5 36.0 41.0 31.0 41.0 38-39 36.94437674298462 39.0 35.5 41.0 30.5 41.0 40-41 36.78058715348473 39.0 35.0 41.0 30.0 41.0 42-43 36.623851015771095 39.0 35.0 41.0 30.0 41.0 44-45 36.43588791484259 39.0 35.0 41.0 29.5 41.0 46-47 36.206384485973885 39.0 35.0 41.0 28.5 41.0 48-49 35.917207192761666 39.0 35.0 41.0 27.0 41.0 50-51 35.2299397131619 38.5 34.0 39.5 26.5 40.5 52-53 35.446972278778546 38.5 34.5 40.0 27.0 40.5 54-55 35.80582631842979 39.0 35.0 40.5 27.0 41.0 56-57 35.70323970218003 39.0 35.0 41.0 27.0 41.0 58-59 35.48589918027842 38.0 35.0 40.0 26.0 41.0 60-61 35.25026750898786 38.0 34.0 40.0 26.0 41.0 62-63 34.999288292556926 38.0 34.0 40.0 26.0 41.0 64-65 34.751733665504716 37.0 34.0 40.0 26.0 41.0 66-67 34.55603267955996 37.0 34.0 40.0 25.5 41.0 68-69 34.248205392869835 36.0 33.5 39.5 25.0 41.0 70-71 33.951131622052614 36.0 33.0 39.0 24.5 41.0 72-73 33.61547473887132 35.0 33.0 39.0 24.0 41.0 74-75 33.242742404080104 35.0 33.0 38.5 23.5 40.0 76-77 32.886669303002066 35.0 32.0 37.0 23.0 39.5 78-79 32.5150068494881 35.0 32.0 37.0 22.0 39.0 80-81 32.17168390166641 35.0 32.0 36.5 20.5 39.0 82-83 31.835661987429113 35.0 31.0 36.0 20.0 37.5 84-85 31.504454461602393 35.0 31.0 35.5 20.0 37.0 86-87 31.23279278145931 35.0 31.0 35.0 19.0 37.0 88-89 30.964189267571374 34.0 31.0 35.0 19.0 36.0 90-91 30.68831580960846 34.0 30.0 35.0 18.0 36.0 92-93 30.437632381760615 34.0 30.0 35.0 17.5 36.0 94-95 30.216832056326147 34.0 29.5 35.0 17.0 35.5 96-97 30.015874623175037 34.0 29.0 35.0 15.5 35.0 98-99 29.78789592077795 34.0 29.0 35.0 14.5 35.0 100 28.122680441605084 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.30486530971257864 1101 2 -0.28508980264606976 1101 3 -0.3051477583295963 1101 4 -0.20571384220136935 1101 5 -0.17577906739114013 1101 6 -0.2572217482633903 1101 7 -0.2706997865515177 1101 8 -0.34761830016041984 1101 9 -0.4534866173488936 1101 10-11 -0.4175172210262943 1101 12-13 -0.3261998758632316 1101 14-15 -0.41128673953932093 1101 16-17 -0.4395059667839618 1101 18-19 -0.4815759949690559 1101 20-21 -0.5499466152469381 1101 22-23 -0.5464306031609354 1101 24-25 -0.529821225348762 1101 26-27 -0.5719813678333878 1101 28-29 -0.5693516655445663 1101 30-31 -0.5623059718948866 1101 32-33 -0.5880028341015588 1101 34-35 -0.6578354035272866 1101 36-37 -0.6471164719193538 1101 38-39 -0.5858412638486641 1101 40-41 -0.5970255418857846 1101 42-43 -0.6654384642702738 1101 44-45 -0.7300789418636384 1101 46-47 -0.6720126663313479 1101 48-49 -0.5618575406189024 1101 50-51 -0.6210364324618141 1101 52-53 -0.40496971628804346 1101 54-55 -0.39897782943311455 1101 56-57 -0.34546518457639763 1101 58-59 -0.5146201958514638 1101 60-61 -0.46264427230681093 1101 62-63 -0.40813982625226686 1101 64-65 -0.41163239437291566 1101 66-67 -0.4973022794141144 1101 68-69 -0.5158528741068906 1101 70-71 -0.5248922172763528 1101 72-73 -0.5747196935469177 1101 74-75 -0.553954142806063 1101 76-77 -0.5699765911265686 1101 78-79 -0.594807707923465 1101 80-81 -0.6489653288068169 1101 82-83 -0.6981084324149656 1101 84-85 -0.6368019379941607 1101 86-87 -0.7000369521896914 1101 88-89 -0.7411508605398893 1101 90-91 -0.7669404246336917 1101 92-93 -0.8256573575863051 1101 94-95 -0.9022874833319854 1101 96-97 -0.8913848726518303 1101 98-99 -0.9081367530054258 1101 100 -0.864693986107067 1102 1 -0.2882150098221601 1102 2 -0.30230481262230313 1102 3 -0.24956441842023125 1102 4 -0.15925971996293242 1102 5 -0.19243963679800657 1102 6 -0.179852018743901 1102 7 -0.22295466569439526 1102 8 -0.191903775323226 1102 9 -0.2717979782373092 1102 10-11 -0.2621218727602823 1102 12-13 -0.26102950323971186 1102 14-15 -0.3478476019985237 1102 16-17 -0.3630304359324654 1102 18-19 -0.35694818172178344 1102 20-21 -0.3870196673601214 1102 22-23 -0.4452847333486858 1102 24-25 -0.3969874593713243 1102 26-27 -0.4144856484865542 1102 28-29 -0.4487243672179275 1102 30-31 -0.5076568463555446 1102 32-33 -0.4895374638954735 1102 34-35 -0.39653664427043367 1102 36-37 -0.5051066238638597 1102 38-39 -0.5586041052875999 1102 40-41 -0.5848645858989059 1102 42-43 -0.5518608065339379 1102 44-45 -0.5147626436608448 1102 46-47 -0.5036367059350155 1102 48-49 -0.5107563913636852 1102 50-51 -0.43771923218469766 1102 52-53 -0.32807789465189785 1102 54-55 -0.2721081382507933 1102 56-57 -0.2439697050121481 1102 58-59 -0.3104241054266268 1102 60-61 -0.3354070528368922 1102 62-63 -0.2817095570551018 1102 64-65 -0.3017464861509751 1102 66-67 -0.3230151641032961 1102 68-69 -0.33860817347130023 1102 70-71 -0.3784670012478628 1102 72-73 -0.434954945929924 1102 74-75 -0.3903198447911791 1102 76-77 -0.38700339840317355 1102 78-79 -0.3819243856507555 1102 80-81 -0.37269379163004146 1102 82-83 -0.4460373223735843 1102 84-85 -0.48272255389545293 1102 86-87 -0.5118573129352804 1102 88-89 -0.6054279821238957 1102 90-91 -0.6342186176328077 1102 92-93 -0.608218619465962 1102 94-95 -0.5790124968583505 1102 96-97 -0.5918844754198496 1102 98-99 -0.603083911527257 1102 100 -0.8583904719224513 1103 1 -0.4286164502281764 1103 2 -0.42710033144111037 1103 3 -0.44841486361742966 1103 4 -0.3121160121425959 1103 5 -0.28060966129326914 1103 6 -0.33778712658375554 1103 7 -0.3020702847254739 1103 8 -0.381124196479 1103 9 -0.5213420246071649 1103 10-11 -0.4814244393470446 1103 12-13 -0.5905805355608678 1103 14-15 -0.6691348642713351 1103 16-17 -0.698484014428594 1103 18-19 -0.6402986445808807 1103 20-21 -0.732641161319691 1103 22-23 -0.7095405467679541 1103 24-25 -0.6370357414667112 1103 26-27 -0.652101758697242 1103 28-29 -0.673323749543357 1103 30-31 -0.678390220892517 1103 32-33 -0.7658015862875089 1103 34-35 -0.818330599005229 1103 36-37 -0.8402409188428166 1103 38-39 -0.8632799753680231 1103 40-41 -0.8081897335573984 1103 42-43 -0.8262760328337109 1103 44-45 -0.896676354446825 1103 46-47 -0.9128869544591609 1103 48-49 -0.7839212349424329 1103 50-51 -0.7883962417928743 1103 52-53 -0.7178512058698558 1103 54-55 -0.6818690587531293 1103 56-57 -0.6428776687507067 1103 58-59 -0.6634346653412067 1103 60-61 -0.7166976018435705 1103 62-63 -0.7416454211707091 1103 64-65 -0.6707796920279989 1103 66-67 -0.651069706894333 1103 68-69 -0.717096181713373 1103 70-71 -0.7581519803403154 1103 72-73 -0.7915546997141192 1103 74-75 -0.7221020261384083 1103 76-77 -0.700600790523346 1103 78-79 -0.7816912499965376 1103 80-81 -0.725573403383521 1103 82-83 -0.7573122797251308 1103 84-85 -0.7667075455859766 1103 86-87 -0.8470953436180224 1103 88-89 -0.8420510437445792 1103 90-91 -0.7624722913355058 1103 92-93 -0.8621396166790696 1103 94-95 -1.0258910833833745 1103 96-97 -1.0457595558904735 1103 98-99 -1.0820703541762242 1103 100 -1.1809988134132325 1104 1 -0.3407145131547651 1104 2 -0.36230085067506224 1104 3 -0.4455259988425304 1104 4 -0.2561636414132522 1104 5 -0.2250960961139512 1104 6 -0.29923880413499404 1104 7 -0.2991965454079448 1104 8 -0.3185947889653349 1104 9 -0.4556235015343333 1104 10-11 -0.4402525749505415 1104 12-13 -0.5045324637625441 1104 14-15 -0.6239812476169533 1104 16-17 -0.5822817401954907 1104 18-19 -0.5857054809084374 1104 20-21 -0.5684554437328657 1104 22-23 -0.5937208281120547 1104 24-25 -0.6352668784739635 1104 26-27 -0.6685570225850341 1104 28-29 -0.6401432497792783 1104 30-31 -0.6798219116504427 1104 32-33 -0.783907065455864 1104 34-35 -0.7115564684499773 1104 36-37 -0.723732538809692 1104 38-39 -0.7699425204753396 1104 40-41 -0.879749366725612 1104 42-43 -0.8170873662706128 1104 44-45 -0.8059764865694348 1104 46-47 -0.8187724971538799 1104 48-49 -0.6924193484005912 1104 50-51 -0.7093990030183122 1104 52-53 -0.5851900571559057 1104 54-55 -0.6335942029008308 1104 56-57 -0.5392070590004465 1104 58-59 -0.6363652825122799 1104 60-61 -0.5422779161541982 1104 62-63 -0.5758671671787923 1104 64-65 -0.5723068516059513 1104 66-67 -0.5885241214145083 1104 68-69 -0.6207549155400116 1104 70-71 -0.5892971943779415 1104 72-73 -0.5640206461060373 1104 74-75 -0.5798548156073196 1104 76-77 -0.5304786236678822 1104 78-79 -0.6476553226718949 1104 80-81 -0.6033389015823545 1104 82-83 -0.6651785115184019 1104 84-85 -0.6397266613202319 1104 86-87 -0.6763033281085882 1104 88-89 -0.6450419823549076 1104 90-91 -0.6069733748479926 1104 92-93 -0.7082206483127713 1104 94-95 -0.7999493849537096 1104 96-97 -0.8190067356409081 1104 98-99 -0.8842006612322102 1104 100 -1.0154839584575868 1105 1 -0.26226096518781006 1105 2 -0.26541858146746833 1105 3 -0.2549589223529303 1105 4 -0.13536703068345446 1105 5 -0.10865079054563864 1105 6 -0.10398340500125869 1105 7 -0.07679579171579576 1105 8 -0.13734467787007532 1105 9 -0.2800750798730718 1105 10-11 -0.3051266957026044 1105 12-13 -0.29722987418374913 1105 14-15 -0.3273305372194315 1105 16-17 -0.3940288618320196 1105 18-19 -0.3621548005590114 1105 20-21 -0.39080363447484956 1105 22-23 -0.3950715013349466 1105 24-25 -0.4165452779485008 1105 26-27 -0.4036021833385348 1105 28-29 -0.49093277862281326 1105 30-31 -0.4873333562081612 1105 32-33 -0.4578520179158545 1105 34-35 -0.4047058780640853 1105 36-37 -0.46329039926111903 1105 38-39 -0.48667546450806043 1105 40-41 -0.6094829621428701 1105 42-43 -0.5604393506435201 1105 44-45 -0.5804770960904051 1105 46-47 -0.5829517579716921 1105 48-49 -0.49568231168014876 1105 50-51 -0.4779572110044725 1105 52-53 -0.38690977205418164 1105 54-55 -0.3879266988988732 1105 56-57 -0.48633543861409123 1105 58-59 -0.37742932763850234 1105 60-61 -0.3882095523016318 1105 62-63 -0.40838746580613616 1105 64-65 -0.3703559791415003 1105 66-67 -0.34887352295444174 1105 68-69 -0.36656561431268386 1105 70-71 -0.3931709385085185 1105 72-73 -0.42109834464446294 1105 74-75 -0.48914037716211567 1105 76-77 -0.4678640620312251 1105 78-79 -0.3816678761006642 1105 80-81 -0.4626216886657133 1105 82-83 -0.4734336335610472 1105 84-85 -0.44334806686307715 1105 86-87 -0.4339251339093373 1105 88-89 -0.4519484113749037 1105 90-91 -0.502000994806977 1105 92-93 -0.5277321131928794 1105 94-95 -0.5188774754471659 1105 96-97 -0.6216889497370381 1105 98-99 -0.5643057276991534 1105 100 -0.5394994052245856 1106 1 -0.38901848880643186 1106 2 -0.36944119888347515 1106 3 -0.3762723424956036 1106 4 -0.31936098385114065 1106 5 -0.2409385922263496 1106 6 -0.2679367275981406 1106 7 -0.3419143856238449 1106 8 -0.33396759395887443 1106 9 -0.441871571495561 1106 10-11 -0.4625236974082654 1106 12-13 -0.4654532248462928 1106 14-15 -0.5758281345029204 1106 16-17 -0.6174833831541875 1106 18-19 -0.6257859763165854 1106 20-21 -0.5082576689852658 1106 22-23 -0.48363654569659076 1106 24-25 -0.5248325175213395 1106 26-27 -0.5440740710528402 1106 28-29 -0.5959021833118712 1106 30-31 -0.6123911847680361 1106 32-33 -0.6130039343525269 1106 34-35 -0.6796317571215198 1106 36-37 -0.6685301073905663 1106 38-39 -0.6825402068827131 1106 40-41 -0.7057263967632466 1106 42-43 -0.6171919946795228 1106 44-45 -0.6180047085528457 1106 46-47 -0.6083331665033072 1106 48-49 -0.6258403919194109 1106 50-51 -0.6328062632319984 1106 52-53 -0.5144321484506449 1106 54-55 -0.5343248025033844 1106 56-57 -0.511501723969765 1106 58-59 -0.46382994875676076 1106 60-61 -0.41324126318952636 1106 62-63 -0.4559635229439394 1106 64-65 -0.4560545786738359 1106 66-67 -0.48205405281989755 1106 68-69 -0.5203392772954487 1106 70-71 -0.5462216228432553 1106 72-73 -0.4947146979466126 1106 74-75 -0.4996889117315533 1106 76-77 -0.5047417855994141 1106 78-79 -0.42098095826405313 1106 80-81 -0.5674816220345242 1106 82-83 -0.5665787558568347 1106 84-85 -0.5235057898190867 1106 86-87 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1.2239857613631742 2311 1 0.5064687293267944 2311 2 0.5090644169319631 2311 3 0.5294939172841744 2311 4 0.4056749885130486 2311 5 0.3617729719034699 2311 6 0.24948765071822265 2311 7 0.3678510852044212 2311 8 0.3740547560246341 2311 9 0.5176067381892722 2311 10-11 0.5286586643630358 2311 12-13 0.5604634633743615 2311 14-15 0.7022337450178853 2311 16-17 0.66654435602711 2311 18-19 0.6131401586483562 2311 20-21 0.6554640014144582 2311 22-23 0.7113522624707898 2311 24-25 0.7025149343524291 2311 26-27 0.7521383724512702 2311 28-29 0.7335437927356949 2311 30-31 0.778133300803816 2311 32-33 0.7980245592422577 2311 34-35 0.8192084967929887 2311 36-37 0.7958754895329321 2311 38-39 0.844445249056271 2311 40-41 0.8494057138773741 2311 42-43 0.789215130195295 2311 44-45 0.902807817516333 2311 46-47 0.9268620918605777 2311 48-49 0.9487747852356421 2311 50-51 0.9018061759579155 2311 52-53 0.7787084588324333 2311 54-55 0.7267223842430397 2311 56-57 0.6950757933097549 2311 58-59 0.6694919289853587 2311 60-61 0.6336982630733914 2311 62-63 0.6197263549687904 2311 64-65 0.660546969657787 2311 66-67 0.6631557906265613 2311 68-69 0.7043814029595197 2311 70-71 0.6419072869736127 2311 72-73 0.6049961092007763 2311 74-75 0.6821312862264506 2311 76-77 0.7156301501131921 2311 78-79 0.699601007041565 2311 80-81 0.7058506719635247 2311 82-83 0.6813835968269153 2311 84-85 0.7548180813019236 2311 86-87 0.7883925282689432 2311 88-89 0.8100752213124132 2311 90-91 0.7720445641798221 2311 92-93 0.7760299145097029 2311 94-95 0.8604937390744887 2311 96-97 0.8748316300634507 2311 98-99 0.8993835684542297 2311 100 1.014848305659303 2312 1 0.4324143792887334 2312 2 0.43157446171991865 2312 3 0.436214946577099 2312 4 0.35018058988158884 2312 5 0.3263047677494484 2312 6 0.3690121335783729 2312 7 0.3370359002278889 2312 8 0.3565782116947034 2312 9 0.5018743138337669 2312 10-11 0.47356424066558134 2312 12-13 0.49010230411907685 2312 14-15 0.6674851454834041 2312 16-17 0.6308051806965409 2312 18-19 0.6033683757722414 2312 20-21 0.60857319933978 2312 22-23 0.6325154636114476 2312 24-25 0.6338655356705019 2312 26-27 0.6825194813370175 2312 28-29 0.6639909020628352 2312 30-31 0.6829100594652218 2312 32-33 0.6914245604777207 2312 34-35 0.7368974764116771 2312 36-37 0.7410701431516955 2312 38-39 0.7175467353343166 2312 40-41 0.7720191933216185 2312 42-43 0.7631658561173609 2312 44-45 0.732064249745342 2312 46-47 0.8018828408836711 2312 48-49 0.7942723530982789 2312 50-51 0.810570341216085 2312 52-53 0.665527246473026 2312 54-55 0.6168358604234854 2312 56-57 0.5844814663004314 2312 58-59 0.5566387207493548 2312 60-61 0.5684536146974111 2312 62-63 0.579352581039835 2312 64-65 0.5834494388528313 2312 66-67 0.6190577760147278 2312 68-69 0.7100271631870143 2312 70-71 0.6895514381945631 2312 72-73 0.7241103281906689 2312 74-75 0.5880751605209724 2312 76-77 0.671007081332597 2312 78-79 0.6513783787882517 2312 80-81 0.6430608284499257 2312 82-83 0.6882805394661311 2312 84-85 0.6723747177366484 2312 86-87 0.7035542346838888 2312 88-89 0.730277288537625 2312 90-91 0.8223696141645505 2312 92-93 0.8264172832081478 2312 94-95 0.8255344372504183 2312 96-97 0.8392525203311543 2312 98-99 0.8457346189032755 2312 100 0.9353519163316228 2313 1 0.4034316504377209 2313 2 0.4062554352231871 2313 3 0.44913847336845336 2313 4 0.3530813597204272 2313 5 0.3664856552094591 2313 6 0.3529571583129041 2313 7 0.36569915708113143 2313 8 0.3609295697379764 2313 9 0.5183613426423932 2313 10-11 0.5138846430183364 2313 12-13 0.5089448731651984 2313 14-15 0.6791463835322915 2313 16-17 0.6361661672158831 2313 18-19 0.6057343103947304 2313 20-21 0.5674136632736335 2313 22-23 0.5739524284976696 2313 24-25 0.5962454296068174 2313 26-27 0.6626279577523846 2313 28-29 0.672434679060764 2313 30-31 0.7420436580964989 2313 32-33 0.7158862343205925 2313 34-35 0.7263053181182855 2313 36-37 0.7199969467594869 2313 38-39 0.7579993113057029 2313 40-41 0.7738490461686567 2313 42-43 0.6263270764114068 2313 44-45 0.769903999519741 2313 46-47 0.8014683887547918 2313 48-49 0.8771524190098816 2313 50-51 0.7824533188825811 2313 52-53 0.6624655247607478 2313 54-55 0.6621665711294682 2313 56-57 0.7271033404451757 2313 58-59 0.686114095120395 2313 60-61 0.7241357804251365 2313 62-63 0.7015025797169443 2313 64-65 0.6919985803943831 2313 66-67 0.7049951910928911 2313 68-69 0.6867633559131079 2313 70-71 0.6806283726530822 2313 72-73 0.7349739947238234 2313 74-75 0.7631466898072574 2313 76-77 0.7380000317072728 2313 78-79 0.7584757469353463 2313 80-81 0.760015368311862 2313 82-83 0.7585364215315984 2313 84-85 0.730560524893594 2313 86-87 0.7607415518496268 2313 88-89 0.7176760260363899 2313 90-91 0.8039295954847248 2313 92-93 0.8242813636679607 2313 94-95 0.8693107463113989 2313 96-97 0.885877692510693 2313 98-99 0.884341017856972 2313 100 1.013469179155134 2314 1 0.3058571461961357 2314 2 0.1672790089885403 2314 3 0.3017169384788083 2314 4 0.20819200818991845 2314 5 0.17793245589162154 2314 6 0.23418527429056724 2314 7 0.28631206032542167 2314 8 0.31951562688286117 2314 9 0.35504831254959157 2314 10-11 0.38931167536043887 2314 12-13 0.3945357418813771 2314 14-15 0.4882998237076137 2314 16-17 0.5369910738088279 2314 18-19 0.5203729220911484 2314 20-21 0.496960277306016 2314 22-23 0.5215923622326173 2314 24-25 0.5649094183637473 2314 26-27 0.6371590823167708 2314 28-29 0.6034195372976612 2314 30-31 0.6012670003460485 2314 32-33 0.6084230558056518 2314 34-35 0.5942079210321864 2314 36-37 0.6490704268682563 2314 38-39 0.719727935090539 2314 40-41 0.7030990285059886 2314 42-43 0.615858764552641 2314 44-45 0.7182982299100189 2314 46-47 0.5252156318945396 2314 48-49 0.46576806785947156 2314 50-51 0.6223110455800125 2314 52-53 0.5596799775730048 2314 54-55 0.590169884683938 2314 56-57 0.5877392583708954 2314 58-59 0.5688408686905646 2314 60-61 0.3928642028420555 2314 62-63 0.5314804521639687 2314 64-65 0.5564831341821801 2314 66-67 0.6155733695298835 2314 68-69 0.6154861421092122 2314 70-71 0.5807938769914998 2314 72-73 0.6097287377778642 2314 74-75 0.667000652360116 2314 76-77 0.6367994539520438 2314 78-79 0.636741477280431 2314 80-81 0.6466276974555782 2314 82-83 0.6626049707198511 2314 84-85 0.696962460455925 2314 86-87 0.6940713790353747 2314 88-89 0.611567011276378 2314 90-91 0.6016015481645312 2314 92-93 0.7914497743019346 2314 94-95 0.7941754358515674 2314 96-97 0.7239724134661216 2314 98-99 0.8119921071870984 2314 100 0.857657691661732 2315 1 0.2283292061998452 2315 2 0.28343062780793815 2315 3 0.26289451351159343 2315 4 0.24099569213295524 2315 5 0.20413381004397735 2315 6 0.20178147743894925 2315 7 0.18980811265374342 2315 8 0.2052555872897841 2315 9 0.05747469862640031 2315 10-11 0.2028450348188997 2315 12-13 0.21400693329972142 2315 14-15 0.3328218915563639 2315 16-17 0.23626726893949268 2315 18-19 0.3485842143014324 2315 20-21 0.22105159963526688 2315 22-23 0.213444813827941 2315 24-25 0.4056236778027582 2315 26-27 0.44373333135077786 2315 28-29 0.344156220803022 2315 30-31 0.40675787119108264 2315 32-33 0.46715798787656837 2315 34-35 0.3394597507294037 2315 36-37 0.47718314552542296 2315 38-39 0.5296191629789533 2315 40-41 0.5741931532804898 2315 42-43 0.5830510867685277 2315 44-45 0.5391376084323127 2315 46-47 0.5268525099119685 2315 48-49 0.4239917207075976 2315 50-51 0.4089602166703088 2315 52-53 0.08942967934869017 2315 54-55 0.30530999568569683 2315 56-57 0.29970076735216367 2315 58-59 0.316557900545952 2315 60-61 0.3744784841270814 2315 62-63 0.38869140730007956 2315 64-65 0.441940898186715 2315 66-67 0.33631806699267486 2315 68-69 0.4375369945165133 2315 70-71 0.37582890943394887 2315 72-73 0.39887521565295714 2315 74-75 0.12071873822920764 2315 76-77 0.3389939157256592 2315 78-79 0.4001334157272396 2315 80-81 0.4906396046573249 2315 82-83 0.4094378584996683 2315 84-85 0.4332411352213832 2315 86-87 0.38252414473278407 2315 88-89 0.3173255520529672 2315 90-91 0.5066090366165525 2315 92-93 0.5424427580540581 2315 94-95 0.5173568680940264 2315 96-97 0.5524953378886828 2315 98-99 0.7177905150558672 2315 100 0.5832896757224013 2316 1 0.15530964723480878 2316 2 0.16863132240043655 2316 3 0.1548369048976106 2316 4 0.07919956868526867 2316 5 0.07998178536328737 2316 6 -0.005148528217105763 2316 7 -0.011657686522369204 2316 8 0.017913860518795843 2316 9 0.09941993901635016 2316 10-11 0.12633327972186947 2316 12-13 0.10848214257946864 2316 14-15 -0.038288602122243276 2316 16-17 0.06801044744388918 2316 18-19 0.1464389295936428 2316 20-21 0.21871067569604463 2316 22-23 0.08467536613424187 2316 24-25 0.08626802180530291 2316 26-27 0.06041432948615011 2316 28-29 0.08103354086286885 2316 30-31 -0.5190700962639099 2316 32-33 -0.29713428636465977 2316 34-35 -0.40739750807114206 2316 36-37 -0.11008056735600036 2316 38-39 -0.10512803654933833 2316 40-41 0.06128302734078517 2316 42-43 0.06702553497901675 2316 44-45 -0.09136870571556699 2316 46-47 -0.22645972223111954 2316 48-49 -0.0995496903464641 2316 50-51 0.09114978107782434 2316 52-53 -0.2537360026254305 2316 54-55 -0.1691745043217452 2316 56-57 -0.17977916166302066 2316 58-59 -0.6082883428688319 2316 60-61 -0.11852449950850286 2316 62-63 -0.1271638488070721 2316 64-65 -0.42748599053698655 2316 66-67 -0.6722682587800577 2316 68-69 -0.689131852445442 2316 70-71 -0.45035562592860146 2316 72-73 -0.7130603640583146 2316 74-75 -0.5296782420554536 2316 76-77 -0.1568314506306976 2316 78-79 -0.1478297867362528 2316 80-81 -0.2436066125939007 2316 82-83 -0.01362407701520496 2316 84-85 0.03225364070867087 2316 86-87 0.1561925862742406 2316 88-89 0.151267989968904 2316 90-91 -0.35055716939122306 2316 92-93 -0.5000725310283052 2316 94-95 -0.04922828222621334 2316 96-97 0.23185901076201176 2316 98-99 0.2516103466404509 2316 100 0.12072217778478134 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 264.0 1 731.0 2 424.0 3 410.0 4 371.0 5 348.0 6 403.0 7 892.0 8 2308.0 9 4216.0 10 6316.0 11 9011.0 12 12441.0 13 15283.0 14 17882.0 15 20940.0 16 25823.0 17 34264.0 18 44900.0 19 57754.0 20 77204.0 21 101935.0 22 112478.0 23 105764.0 24 93669.0 25 89970.0 26 94853.0 27 107754.0 28 127053.0 29 155390.0 30 191005.0 31 241251.0 32 312345.0 33 416665.0 34 598870.0 35 849462.0 36 1271122.0 37 1885530.0 38 1995885.0 39 614040.0 40 579.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.39161646408229 21.365187804835788 14.092912668544125 22.150283062537802 2 14.76096259715051 20.718660092778084 42.52749726784756 21.992880042223845 3 18.29112800683236 27.89538674341694 29.91506268068273 23.89842256906797 4 11.507443676098244 17.643507990284803 41.0420012066645 29.80704712695245 5 12.05105745381668 39.27181566709251 35.442125029354955 13.235001849735855 6 27.879226914592977 39.73632417378472 18.816737295348247 13.567711616274053 7 25.74027820518997 33.60006949094683 23.585543035745438 17.074109268117756 8 25.090429208771113 35.94463695090399 20.81895208471756 18.14598175560733 9 26.372255151878303 15.833666079705846 22.27463185323002 35.51944691518583 10-11 24.25479942883683 27.467940411083784 28.255788674784366 20.021471485295024 12-13 24.866523871953486 25.450741265273802 28.80988812385458 20.87284673891813 14-15 22.45972216241 26.394849552989946 27.154914918652658 23.9905133659474 16-17 20.283122315252328 29.35466820381935 28.329504893381696 22.03270458754663 18-19 19.820824508883316 28.4863665148471 31.385857890242015 20.30695108602757 20-21 21.45193934149338 27.503470515679012 30.974630984318303 20.069959158509302 22-23 21.43771714631735 27.562856563060933 30.515339787916325 20.484086502705388 24-25 20.756779708455834 27.725030446035387 31.360786228718407 20.157403616790372 26-27 20.609986917708678 27.9682611922616 30.764860838602782 20.656891051426935 28-29 20.07629788632756 28.013392005074284 31.16329773920802 20.747012369390138 30-31 20.805535129870815 27.797732332026293 31.083346508652248 20.31338602945064 32-33 20.95460405072944 28.151226434636783 30.41254395745422 20.48162555717956 34-35 20.965311184518896 28.014609617809256 30.9830857587227 20.036993438949143 36-37 20.7778076309299 27.712175060488736 30.778074297692847 20.731943010888514 38-39 20.814192786505465 27.951918633438073 31.05200762772832 20.181880952328143 40-41 20.725261237890138 27.33187335947681 31.379137760460218 20.56372764217283 42-43 20.526144192088285 27.513632081847174 30.808995058918455 21.15122866714609 44-45 20.648778313999134 28.247840746900525 30.549382870817908 20.553998068282432 46-47 20.71677759777361 27.977374779851445 30.07716046357361 21.228687158801335 48-49 21.127804855500298 27.907090841442724 30.02240335027746 20.942700952779518 50-51 21.145440625534082 27.910626550852164 29.55343556716573 21.39049725644802 52-53 20.57548649992251 28.547344321985264 29.460013409586512 21.417155768505715 54-55 20.764730472906567 28.485264317076087 29.32877644238556 21.42122876763178 56-57 21.221043978058972 28.205109025486934 29.020337561183666 21.553509435270424 58-59 20.651205729142085 28.283966136918288 29.695189242166244 21.369638891773384 60-61 20.905175148615026 28.80425424664838 29.056549795692703 21.23402080904389 62-63 20.94625038006861 28.304347695997528 29.124707797269366 21.624694126664497 64-65 20.47380468870986 28.230685589718334 29.231617545783 22.06389217578881 66-67 20.933992034121705 27.971451553339044 29.02664077712373 22.06791563541552 68-69 20.61129820390914 28.129276652469628 29.127397814315902 22.132027329305334 70-71 20.801548179785797 27.71144521404732 29.352576454609274 22.13443015155761 72-73 21.162896659185908 27.97967267572495 29.180820552747793 21.67661011234135 74-75 21.17697879016165 28.187794897761897 28.910571038236142 21.724655273840305 76-77 20.871541637463153 28.130643551258462 28.997466200400908 22.000348610877474 78-79 20.801238738796833 28.031557215470865 29.015951950273806 22.1512520954585 80-81 20.63298263933135 28.358240294436442 29.054426271457075 21.954350794775134 82-83 21.323199416961238 27.784940696398703 29.598301437396067 21.293558449243996 84-85 21.35480335017445 27.981623688939255 28.809164363084687 21.8544085978016 86-87 20.606869551486298 28.411758949389053 29.218970429294476 21.76240106983017 88-89 21.341813858646585 27.65692091389416 29.03999860496858 21.961266622490673 90-91 21.228188914643596 27.649352218620137 29.262374444084777 21.86008442265149 92-93 21.375334369799738 27.84197560981147 28.992031261576916 21.790658758811873 94-95 20.607720530225453 27.681500445858553 29.384106342295325 22.326672681620668 96-97 21.00519403446822 27.63190529332103 29.10729857631127 22.25560209589948 98-99 20.716550359494505 27.903457934805893 28.95162860399453 22.428363101705077 100 21.30801563606512 27.59176369515971 28.314344750642633 22.785875918132533 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4235.0 1 2849.5 2 2357.0 3 3047.5 4 2337.5 5 2079.5 6 3174.5 7 6154.0 8 9660.5 9 11256.0 10 10085.0 11 8595.0 12 9117.5 13 10428.0 14 11947.0 15 13405.0 16 14952.5 17 16458.5 18 17931.5 19 19560.5 20 21349.5 21 23306.0 22 26088.0 23 30662.5 24 36052.5 25 43170.5 26 54078.5 27 66397.0 28 78830.5 29 98629.0 30 128101.5 31 156889.5 32 195727.5 33 241084.0 34 280653.0 35 317557.0 36 357686.0 37 404584.5 38 414831.0 39 393890.5 40 367369.0 41 335770.0 42 318855.5 43 319281.5 44 327679.5 45 356587.5 46 376048.0 47 373773.0 48 372127.0 49 361249.5 50 343341.0 51 329968.0 52 313506.5 53 288931.0 54 264946.5 55 227038.5 56 194109.0 57 157706.0 58 120352.5 59 105490.0 60 84594.5 61 58873.0 62 42751.5 63 34236.5 64 24792.0 65 13639.5 66 8503.0 67 6301.0 68 5046.5 69 4140.0 70 3288.5 71 2430.5 72 1887.0 73 1563.5 74 1263.5 75 929.0 76 650.0 77 477.0 78 352.5 79 251.5 80 167.5 81 114.5 82 76.0 83 53.0 84 39.5 85 36.0 86 32.0 87 23.5 88 17.0 89 14.0 90 12.5 91 8.5 92 7.5 93 5.5 94 6.0 95 5.0 96 2.0 97 1.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024964412049943263 2 0.1071170228727016 3 0.0164573323551051 4 0.01706571744843292 5 0.018829003057908467 6 0.04523704075303638 7 0.04594854196387739 8 0.038751036961456736 9 0.03334775240376559 10-11 0.03663200074656069 12-13 0.0357658253594499 14-15 0.03209489157598034 16-17 0.03806015897411837 18-19 0.03734865776327736 20-21 0.048681119077976925 22-23 0.04891828614825726 24-25 0.06679346511916871 26-27 0.05872978472963727 28-29 0.05163024003885415 30-31 0.04957307349446601 32-33 0.044762706612475715 34-35 0.046036190663763604 36-37 0.041849676292728094 38-39 0.04626304612229262 40-41 0.055620833786614594 42-43 0.05769346774862972 44-45 0.060962248673797834 46-47 0.062426497542485124 48-49 0.06136440153209927 50-51 0.05518259028718354 52-53 0.06425165282246859 54-55 0.050929050439764464 56-57 0.05536819929870729 58-59 0.05346055112471327 60-61 0.04957822930034167 62-63 0.05646638595022276 64-65 0.05452780294097479 66-67 0.052470636396586647 68-69 0.05820904833619566 70-71 0.05825029478320094 72-73 0.060075450063184405 74-75 0.051960211614896355 76-77 0.05206848353828521 78-79 0.061993409848929724 80-81 0.06259148333050624 82-83 0.05299137279002826 84-85 0.058291541230206224 86-87 0.05594564955678115 88-89 0.06462287084551607 90-91 0.07016020635597436 92-93 0.07410955365672954 94-95 0.07746082747590821 96-97 0.07391878883933015 98-99 0.08268365882795127 100 0.1262966207301549 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9697805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.904054512136035 #Duplication Level Percentage of deduplicated Percentage of total 1 69.52017510376129 36.08378958278869 2 16.857771003933443 17.49973330282535 3 6.3535456292298615 9.893243360546714 4 3.0040367802981525 6.236867552042278 5 1.5400548396261389 3.9967545173817016 6 0.8823405436555122 2.747823100569807 7 0.5424237418581832 1.9707794026258767 8 0.3308477230614138 1.373787060239657 9 0.22585784980721874 1.0550644333548942 >10 0.6563846648389238 5.357129268083438 >50 0.03558482854917841 1.3056609225559204 >100 0.04081500174008946 4.6455423215475005 >500 0.0066623229145657825 2.411232504733559 >1k 0.0033157579512516936 2.9964107919267424 >5k 9.21043874903121E-5 0.36020160778116056 >10k+ 9.21043874903121E-5 2.0659802709968105 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56278 0.5803168861407298 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44303 0.456835335418685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29067 0.2997276187755889 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9712 0.10014637332880996 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.0311611751318985E-5 0.0 0.0 2.062322350263797E-5 0.0 8 2.062322350263797E-5 0.0 0.0 2.062322350263797E-5 0.0 9 2.062322350263797E-5 0.0 0.0 2.062322350263797E-5 0.0 10-11 2.062322350263797E-5 0.0 0.0 2.062322350263797E-5 0.0 12-13 2.062322350263797E-5 0.0 0.0 2.062322350263797E-5 0.0 14-15 2.062322350263797E-5 0.0 0.0 4.124644700527594E-5 0.0 16-17 5.155805875659492E-5 0.0 0.0 6.186967050791391E-5 0.0 18-19 9.796031163753036E-5 0.0 0.0 7.218128225923289E-5 0.0 20-21 1.1342772926450883E-4 0.0 0.0 1.5467417626978478E-4 0.0 22-23 1.1342772926450883E-4 0.0 0.0 4.382434994310568E-4 0.0 24-25 1.288951468914873E-4 0.0 0.0 9.486682811213466E-4 0.0 26-27 1.392067586428063E-4 0.0 0.0 0.0021551268560256677 0.0 28-29 1.5467417626978478E-4 0.0 0.0 0.005449686810572083 0.0 30-31 1.6498578802110375E-4 0.0 0.0 0.01756067481249623 0.0 32-33 1.6498578802110375E-4 0.0 0.0 0.04566497264071612 0.0 34-35 1.7529739977242272E-4 0.0 0.0 0.08895827457862887 0.0 36-37 1.8560901152374172E-4 0.0 0.0 0.16093848040871106 0.0 38-39 1.8560901152374172E-4 0.0 0.0 0.2625284793827057 0.0 40-41 1.8560901152374172E-4 5.155805875659492E-6 0.0 0.3713520740002506 0.0 42-43 1.959206232750607E-4 1.0311611751318985E-5 0.0 0.4812893226869379 0.0 44-45 1.959206232750607E-4 1.0311611751318985E-5 0.0 0.6149484342075346 0.0 46-47 1.959206232750607E-4 1.0311611751318985E-5 0.0 0.7739328641893707 0.0 48-49 2.4747868203165563E-4 2.062322350263797E-5 0.0 0.9499727000078884 0.0 50-51 2.577902937829746E-4 2.062322350263797E-5 0.0 1.1323335538299646 0.0 52-53 2.629460996586341E-4 2.062322350263797E-5 0.0 1.3089508399065561 0.0 54-55 2.9388093491259103E-4 2.062322350263797E-5 0.0 1.5004993397990578 0.0 56-57 3.299715760422075E-4 2.062322350263797E-5 0.0 1.7226836382047277 0.0 58-59 4.0215285830144036E-4 2.062322350263797E-5 0.0 1.9781228845084016 0.0 60-61 4.0215285830144036E-4 2.062322350263797E-5 0.0 2.2448842805150235 0.0 62-63 4.1762027592841884E-4 3.0934835253956954E-5 0.0 2.5106557617935192 0.0 64-65 4.330876935553973E-4 3.0934835253956954E-5 0.0 2.7823512640231476 0.0 66-67 4.382434994310568E-4 3.0934835253956954E-5 0.0 3.0818210925049536 0.0 68-69 4.433993053067163E-4 3.0934835253956954E-5 0.0 3.4129372574515573 0.0 70-71 4.433993053067163E-4 3.0934835253956954E-5 0.0 3.776622648114702 0.0 72-73 4.640225288093543E-4 3.0934835253956954E-5 0.0 4.137255801699457 0.0 74-75 4.8464575231199224E-4 3.0934835253956954E-5 0.0 4.481905957069667 0.0 76-77 4.8464575231199224E-4 3.0934835253956954E-5 0.0 4.8666425031231295 0.0 78-79 4.898015581876517E-4 3.0934835253956954E-5 0.0 5.278591392588323 0.0 80-81 5.104247816902897E-4 3.0934835253956954E-5 0.0 5.72809517205182 0.0 82-83 5.258921993172682E-4 3.0934835253956954E-5 0.0 6.18317753347278 0.0 84-85 5.258921993172682E-4 3.0934835253956954E-5 0.0 6.615502167758581 0.0 86-87 5.619828404468847E-4 3.0934835253956954E-5 0.0 7.067594161771659 0.0 88 5.774502580738631E-4 3.0934835253956954E-5 0.0 7.419421198920786 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21700 0.0 55.536465 1 GTATCAA 42105 0.0 43.72663 1 ATCAACG 51305 0.0 34.9964 3 CAACGCA 51760 0.0 34.678608 5 TATCAAC 53745 0.0 33.905075 2 AACGCAG 55605 0.0 32.636063 6 GTACTAG 6040 0.0 32.3199 1 TCAACGC 56690 0.0 31.804213 4 GCATCGT 5470 0.0 30.66747 8 ACGCAGA 61320 0.0 29.128153 7 CGCAGAG 61500 0.0 29.026274 8 GTGGTAT 8720 0.0 28.48238 1 GCAGAGT 65680 0.0 26.723043 9 CTGTGCG 6510 0.0 26.708479 9 TAGGCAT 8020 0.0 26.658127 5 GTACATG 55135 0.0 26.123817 1 TGGTATC 9525 0.0 25.580435 2 TAACGCC 3330 0.0 25.54075 4 TACATGG 56145 0.0 25.25919 2 TCTAACG 3460 0.0 24.742208 2 >>END_MODULE