FastQCFastQC Report
Mon 6 Jun 2016
ERR1211272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9697805
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT474340.4891209918120647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407170.41985789567845505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273490.28201226978682287No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA220400.057.063911
GTATCAA410250.041.9240951
ATCAACG520950.032.165113
CAACGCA528600.031.6488635
TATCAAC548250.031.1324022
AACGCAG567900.029.8634766
TCAACGC574450.029.2678174
GTGGTAT82150.028.7913021
GTACTAG58200.027.173481
ACGCAGA636850.026.2692767
CGCAGAG639450.026.1698028
TGGTATC89950.026.079392
GTACATG535050.024.7953221
TAGGCAT75150.024.7212315
GCTATTG97200.024.5671868
CTGTGCG65950.024.398759
GCAGAGT684000.024.176389
TACATGG544300.024.0574742
ACATGGG530050.023.8491023
GAACCGT9650.023.3355396
Produced by FastQC (version 0.11.5)