##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211272_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9697805 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.979493091477917 33.0 31.0 34.0 30.0 34.0 2 32.123278308854424 33.0 31.0 34.0 30.0 34.0 3 32.17739993740852 34.0 31.0 34.0 30.0 34.0 4 35.85057629020175 37.0 35.0 37.0 35.0 37.0 5 35.76221763584646 37.0 35.0 37.0 35.0 37.0 6 35.783493584372955 37.0 35.0 37.0 33.0 37.0 7 35.69990848444571 37.0 35.0 37.0 33.0 37.0 8 35.68706619693838 37.0 35.0 37.0 33.0 37.0 9 37.26781720193384 39.0 37.0 39.0 34.0 39.0 10-11 37.21495714751947 39.0 37.0 39.0 33.0 39.0 12-13 37.13995166947572 39.0 37.0 39.0 33.0 39.0 14-15 38.29540086648474 40.0 38.0 41.0 33.0 41.0 16-17 38.2977702170749 40.0 38.0 41.0 33.0 41.0 18-19 38.354820034018005 40.0 38.0 41.0 33.5 41.0 20-21 38.34339316989772 40.0 38.0 41.0 34.0 41.0 22-23 38.22794070410778 40.0 38.0 41.0 33.5 41.0 24-25 38.133443289486635 40.0 37.0 41.0 33.0 41.0 26-27 38.01206010019793 40.0 37.0 41.0 33.0 41.0 28-29 37.915741036244796 40.0 37.0 41.0 33.0 41.0 30-31 37.884827442911046 40.0 37.0 41.0 33.0 41.0 32-33 37.775933780891656 40.0 37.0 41.0 33.0 41.0 34-35 37.62819411196658 40.0 36.0 41.0 33.0 41.0 36-37 37.471191161298876 40.0 36.0 41.0 32.0 41.0 38-39 37.340230495457476 40.0 36.0 41.0 32.0 41.0 40-41 37.21749963007093 39.0 36.0 41.0 31.0 41.0 42-43 37.08068021578079 39.0 36.0 41.0 31.0 41.0 44-45 36.86967251867819 39.0 35.0 41.0 30.5 41.0 46-47 36.562708829472236 39.0 35.0 41.0 30.0 41.0 48-49 34.96275523172512 38.5 34.5 40.0 22.5 41.0 50-51 35.66080607931382 38.0 34.5 40.0 27.0 41.0 52-53 35.74967165250281 38.0 35.0 40.0 28.0 41.0 54-55 35.58841150136551 38.0 34.5 40.0 27.0 41.0 56-57 35.36995613955942 38.0 34.0 40.0 27.0 41.0 58-59 35.13102573211154 38.0 34.0 40.0 26.0 41.0 60-61 34.897578833560786 37.5 34.0 40.0 26.0 41.0 62-63 34.59327543707055 37.0 33.5 40.0 26.0 41.0 64-65 34.28023707426577 36.5 33.0 39.5 26.0 41.0 66-67 33.9701344273266 36.0 33.0 39.0 25.0 40.5 68-69 33.64321338694684 35.5 32.5 39.0 24.5 40.0 70-71 33.32910555532928 35.0 32.0 38.5 24.0 40.0 72-73 32.978020851110124 35.0 32.0 38.0 23.5 40.0 74-75 32.615869467369166 35.0 31.0 37.0 23.0 39.0 76-77 31.695990536002732 34.0 30.5 36.0 22.0 39.0 78-79 32.14716031101884 34.5 31.0 36.0 23.0 39.0 80-81 32.018869476134036 35.0 31.0 36.0 23.0 38.0 82-83 31.730864767852104 34.0 31.0 35.5 22.5 37.0 84-85 31.42249777140291 34.0 31.0 35.0 21.5 37.0 86-87 31.138924787619466 34.0 31.0 35.0 20.0 36.0 88-89 30.880385045894407 34.0 30.0 35.0 20.0 36.0 90-91 30.636505735060666 34.0 30.0 35.0 20.0 36.0 92-93 30.41680009032972 34.0 29.5 35.0 19.0 35.0 94-95 30.208541314245853 34.0 29.0 35.0 19.0 35.0 96-97 30.00274593065132 34.0 29.0 35.0 18.0 35.0 98-99 29.77338098672844 33.5 29.0 35.0 18.0 35.0 100 27.336713823385807 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3391951266432969 1101 2 -0.33100544805870413 1101 3 -0.33884062149024174 1101 4 -0.17149843941814424 1101 5 -0.2203616546955658 1101 6 -0.24641782289074143 1101 7 -0.26604444990638143 1101 8 -0.2842429696732225 1101 9 -0.39648781456005366 1101 10-11 -0.3648971287087974 1101 12-13 -0.5146584761139579 1101 14-15 -0.6056312679157685 1101 16-17 -0.605888716692391 1101 18-19 -0.6508823498657677 1101 20-21 -0.6648135580190271 1101 22-23 -0.7141140486143769 1101 24-25 -0.6779611379772348 1101 26-27 -0.7263556611307465 1101 28-29 -0.7742134390949147 1101 30-31 -0.7353762997801923 1101 32-33 -0.6333772219058886 1101 34-35 -0.6356845373822253 1101 36-37 -0.6367636460023292 1101 38-39 -0.6509289934568443 1101 40-41 -0.5684897169579344 1101 42-43 -0.57962150088985 1101 44-45 -0.6023212468918615 1101 46-47 -0.5777141699623272 1101 48-49 0.5796147841887773 1101 50-51 -0.31027340938833703 1101 52-53 -0.6247747317477348 1101 54-55 -0.5046212433075112 1101 56-57 -0.5603518818505719 1101 58-59 -0.6988230237292825 1101 60-61 -0.72625653462147 1101 62-63 -0.7493146163319508 1101 64-65 -0.7383720111124248 1101 66-67 -0.7554830990416548 1101 68-69 -0.738833984575983 1101 70-71 -0.6650817401663147 1101 72-73 -0.7623653493617084 1101 74-75 -0.8599578015627962 1101 76-77 -0.7509588317293243 1101 78-79 -0.5935319212243684 1101 80-81 -0.5193656041522523 1101 82-83 -0.5894386976440842 1101 84-85 -0.6284715873778772 1101 86-87 -0.6633601452893778 1101 88-89 -0.6637559537430775 1101 90-91 -0.6819477527269484 1101 92-93 -0.6768212654409105 1101 94-95 -0.7784311572841602 1101 96-97 -0.8905993334558175 1101 98-99 -1.060637228258333 1101 100 -0.8564972233015382 1102 1 -0.3213424859320462 1102 2 -0.3128617055095013 1102 3 -0.34591949124907373 1102 4 -0.17074445860298937 1102 5 -0.18761703945276764 1102 6 -0.25984396205668503 1102 7 -0.21432277397285304 1102 8 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-0.6106154049373131 1102 72-73 -0.6855836381891507 1102 74-75 -0.7253350504080665 1102 76-77 -0.6469747437855524 1102 78-79 -0.4505792253226133 1102 80-81 -0.4373086477709158 1102 82-83 -0.4133852255042818 1102 84-85 -0.34343683809636616 1102 86-87 -0.4652884049404875 1102 88-89 -0.42569501380922503 1102 90-91 -0.37455243454708764 1102 92-93 -0.6025791702737102 1102 94-95 -0.639906875182497 1102 96-97 -0.6977514482389253 1102 98-99 -0.7391673607264551 1102 100 -0.680092144165851 1103 1 -0.3455730447936176 1103 2 -0.35082380307673233 1103 3 -0.37527947605089906 1103 4 -0.19678407647553797 1103 5 -0.24363367673696956 1103 6 -0.2522756292358821 1103 7 -0.34109173041515106 1103 8 -0.39420404915980356 1103 9 -0.4837372141767986 1103 10-11 -0.45893379008985136 1103 12-13 -0.5625761768112127 1103 14-15 -0.6363968472743977 1103 16-17 -0.6491470042061138 1103 18-19 -0.7104032788584576 1103 20-21 -0.7353867354684027 1103 22-23 -0.773595613584078 1103 24-25 -0.7610998765090358 1103 26-27 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-0.9801820764543443 1103 92-93 -0.8691622321671524 1103 94-95 -0.9243452983729696 1103 96-97 -1.1062366599206825 1103 98-99 -1.217695355561137 1103 100 -1.2374673922009372 1104 1 -0.4158441632499539 1104 2 -0.397829904895346 1104 3 -0.4380208908970573 1104 4 -0.27404903913559053 1104 5 -0.29637038916904146 1104 6 -0.3692772873763417 1104 7 -0.34600050592391796 1104 8 -0.3700246742279063 1104 9 -0.5446803724570586 1104 10-11 -0.500230854239966 1104 12-13 -0.5347056605404603 1104 14-15 -0.6399338553234273 1104 16-17 -0.7302407568373823 1104 18-19 -0.6399536590943313 1104 20-21 -0.6562107514270608 1104 22-23 -0.6761176588336468 1104 24-25 -0.7098715555413122 1104 26-27 -0.7572493169503858 1104 28-29 -0.7504093251102475 1104 30-31 -0.6769104220869835 1104 32-33 -0.6678971225637369 1104 34-35 -0.7061400792318793 1104 36-37 -0.7266762878422384 1104 38-39 -0.7649569195272932 1104 40-41 -0.7983128900388934 1104 42-43 -0.7835668807112341 1104 44-45 -0.8597650082647732 1104 46-47 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1105 68-69 -0.6311177657878844 1105 70-71 -0.5925579218711974 1105 72-73 -0.6618969732624223 1105 74-75 -0.7114488896140614 1105 76-77 -0.6953868909907222 1105 78-79 -0.5886695339244312 1105 80-81 -0.578200891688045 1105 82-83 -0.5981029925245132 1105 84-85 -0.5652438820249941 1105 86-87 -0.549322141460852 1105 88-89 -0.5864896810130809 1105 90-91 -0.6901902944260421 1105 92-93 -0.5830276932302958 1105 94-95 -0.5884688486721963 1105 96-97 -0.6862948635457791 1105 98-99 -0.7133422743381566 1105 100 -0.8078740082522877 1106 1 -0.3366151258093204 1106 2 -0.32446735022982054 1106 3 -0.36206781037781965 1106 4 -0.2025064766363016 1106 5 -0.2898814889360608 1106 6 -0.3879651693626016 1106 7 -0.365803349457849 1106 8 -0.3760145630461622 1106 9 -0.492914415383261 1106 10-11 -0.5004570393795618 1106 12-13 -0.5279129475112185 1106 14-15 -0.6254235688719092 1106 16-17 -0.5937044569082985 1106 18-19 -0.6741417036396342 1106 20-21 -0.6405968311252863 1106 22-23 -0.6671205100282549 1106 24-25 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1107 46-47 -0.8679393189431011 1107 48-49 0.3746752541803602 1107 50-51 -0.5173953221273848 1107 52-53 -0.75137816800531 1107 54-55 -0.8064233114708799 1107 56-57 -0.8483344628301879 1107 58-59 -0.8356241846690935 1107 60-61 -0.9167287208212755 1107 62-63 -0.9531392624094579 1107 64-65 -1.0406352877150624 1107 66-67 -0.9784049307787868 1107 68-69 -0.9191582312508544 1107 70-71 -1.0451094461773138 1107 72-73 -0.9659078453593324 1107 74-75 -0.9008114155043572 1107 76-77 -0.9369473451906138 1107 78-79 -0.8503275234028216 1107 80-81 -0.6687321531542771 1107 82-83 -0.6881491670257383 1107 84-85 -0.7355904423058561 1107 86-87 -0.8014596383108668 1107 88-89 -0.7956398987635254 1107 90-91 -0.7653143696134741 1107 92-93 -0.7276002797328154 1107 94-95 -0.7674154886554803 1107 96-97 -0.8828633791608489 1107 98-99 -0.9188873570392211 1107 100 -0.9063464633698501 1108 1 -0.4224595871300387 1108 2 -0.39840609413244366 1108 3 -0.4188889741777224 1108 4 -0.273735330563305 1108 5 -0.29641822989042055 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0.23088256665542417 2311 9 0.5114555433427697 2311 10-11 0.4338939413681473 2311 12-13 0.48572562669664876 2311 14-15 0.5037486422642132 2311 16-17 0.5394239548186945 2311 18-19 0.696161755420178 2311 20-21 0.775206805986862 2311 22-23 0.7599786961392283 2311 24-25 0.7155034957333726 2311 26-27 0.718317609382332 2311 28-29 0.7892971788920917 2311 30-31 0.6354251853086978 2311 32-33 0.7184242305022437 2311 34-35 0.8191030066290708 2311 36-37 0.8589838852114013 2311 38-39 0.8831851296311442 2311 40-41 0.8548660417044331 2311 42-43 0.8653440425664343 2311 44-45 0.8638281596461397 2311 46-47 0.9206309291730221 2311 48-49 -5.779274132602467 2311 50-51 -0.9179603731827441 2311 52-53 0.4072370659621356 2311 54-55 0.8914019940676212 2311 56-57 0.9598188011733342 2311 58-59 0.967930193717585 2311 60-61 0.8270225124146222 2311 62-63 0.7189993708968316 2311 64-65 0.8692664838804873 2311 66-67 1.0196533256230538 2311 68-69 1.0137604443303943 2311 70-71 1.0031054767836523 2311 72-73 0.5317069933240859 2311 74-75 0.8195831405363947 2311 76-77 0.8361428638770185 2311 78-79 0.8215624360424698 2311 80-81 0.8475790483810677 2311 82-83 0.814549440903253 2311 84-85 0.9073630605867038 2311 86-87 0.9570796639949926 2311 88-89 0.977305260685263 2311 90-91 1.0105925624004293 2311 92-93 1.0513836822420934 2311 94-95 1.0286736274631743 2311 96-97 1.064994434354265 2311 98-99 1.1064878749955795 2311 100 1.294370673943682 2312 1 0.060029548852472914 2312 2 0.2440909921291805 2312 3 0.24425673150386018 2312 4 0.023635697964884628 2312 5 0.24848535775536362 2312 6 0.1413897919615863 2312 7 0.34611839215329354 2312 8 0.3584459287508608 2312 9 0.33980830446976285 2312 10-11 0.2990988302694504 2312 12-13 0.3254553522223844 2312 14-15 0.4019829151256147 2312 16-17 0.5537818011718372 2312 18-19 0.3917412948598411 2312 20-21 0.3974603923717197 2312 22-23 0.6507824664066817 2312 24-25 0.7731074725787863 2312 26-27 0.6508100875927951 2312 28-29 0.7799800953540839 2312 30-31 0.8536530098529553 2312 32-33 0.8524489928234331 2312 34-35 0.434860134780223 2312 36-37 0.7127944200186107 2312 38-39 0.8191377671539826 2312 40-41 0.5934628594244131 2312 42-43 0.6799086937521466 2312 44-45 0.8181212260605548 2312 46-47 0.8853594248026937 2312 48-49 -4.462246278235025 2312 50-51 -0.637402212600314 2312 52-53 0.6371345462848339 2312 54-55 0.8921935058138217 2312 56-57 1.0006326470358502 2312 58-59 1.0460168435485642 2312 60-61 1.0423111162407608 2312 62-63 0.8654241681422974 2312 64-65 1.0004354315413053 2312 66-67 0.9953275498643208 2312 68-69 0.9215238881450674 2312 70-71 0.929333720240848 2312 72-73 1.0674401704237368 2312 74-75 0.7705746962690938 2312 76-77 0.8707766691327485 2312 78-79 0.5723932766007067 2312 80-81 0.7259153145720383 2312 82-83 0.8149680234879391 2312 84-85 0.8487955059547261 2312 86-87 0.9255485640870909 2312 88-89 0.9786870548738662 2312 90-91 0.7876762268239048 2312 92-93 0.9374993554228759 2312 94-95 1.029351451439048 2312 96-97 1.0819953432514282 2312 98-99 1.1562434472844423 2312 100 0.8607404025733665 2313 1 0.12564626347765184 2313 2 0.264733289483722 2313 3 0.32156809579696244 2313 4 0.09527027821349776 2313 5 0.19153618707271391 2313 6 0.32372080574636186 2313 7 0.23675421192656643 2313 8 0.2805247400824413 2313 9 0.5061120587919703 2313 10-11 0.29617931985311685 2313 12-13 0.10694737957950906 2313 14-15 0.19615265549374783 2313 16-17 0.15812374059822787 2313 18-19 0.4807058847035819 2313 20-21 0.4384575574048597 2313 22-23 0.5266262133475834 2313 24-25 0.3634841070200707 2313 26-27 0.4883790164609323 2313 28-29 0.4403406817087827 2313 30-31 0.6163657665164948 2313 32-33 0.5514025459782488 2313 34-35 0.47521357670206754 2313 36-37 -0.207408599886989 2313 38-39 0.23669041066739283 2313 40-41 0.4278888868171222 2313 42-43 0.5893558380475312 2313 44-45 0.6450053485285636 2313 46-47 0.5346291256698592 2313 48-49 -5.181062020562532 2313 50-51 -1.209520181612973 2313 52-53 0.20286228137987194 2313 54-55 0.3202113848924171 2313 56-57 0.40903503673794006 2313 58-59 0.6342355307917416 2313 60-61 0.8593529681694392 2313 62-63 0.9264384293689076 2313 64-65 0.9577838409622075 2313 66-67 1.0131093201428456 2313 68-69 1.0229759929324658 2313 70-71 0.9225097873220776 2313 72-73 0.7200649447936698 2313 74-75 0.8276299663898925 2313 76-77 0.8530416620799492 2313 78-79 0.7775099751280266 2313 80-81 -0.08147334489926905 2313 82-83 0.33502265453432756 2313 84-85 0.6889681093583917 2313 86-87 0.8319012692886858 2313 88-89 0.8212900265802077 2313 90-91 0.8449437993812943 2313 92-93 0.9234009792965985 2313 94-95 0.8958890594784208 2313 96-97 0.8678931936949184 2313 98-99 1.006778577455858 2313 100 1.0200100809641164 2314 1 0.12419528677846259 2314 2 0.2030955013814406 2314 3 0.2754860841779916 2314 4 0.10617645521178787 2314 5 0.20670900871324704 2314 6 0.3015098319431573 2314 7 0.18854012183586377 2314 8 0.3066553471632645 2314 9 0.4756654074018911 2314 10-11 0.3523764918733221 2314 12-13 0.25780993334735314 2314 14-15 0.2689314857440124 2314 16-17 0.4830956959406194 2314 18-19 0.5288503305361161 2314 20-21 0.6059532836137365 2314 22-23 0.31959196762583986 2314 24-25 0.4599794776840227 2314 26-27 0.5303546930087037 2314 28-29 0.6768557109223536 2314 30-31 0.5077547034757686 2314 32-33 0.5084582085249494 2314 34-35 0.38613681780631026 2314 36-37 0.5131367977662364 2314 38-39 0.5849294282537372 2314 40-41 0.39136804015763715 2314 42-43 0.41335808086629555 2314 44-45 0.6075719084171638 2314 46-47 0.6914003108146858 2314 48-49 -6.058476313384098 2314 50-51 -1.2966956526376308 2314 52-53 -0.6200086389562003 2314 54-55 -0.20807931742535857 2314 56-57 0.08973708131575364 2314 58-59 0.5908605839870305 2314 60-61 0.7720745931077531 2314 62-63 0.6456390750609202 2314 64-65 0.824896463820231 2314 66-67 0.8625966385932244 2314 68-69 0.887102378403533 2314 70-71 0.6804078691073059 2314 72-73 0.8653043004658159 2314 74-75 0.8798200172894113 2314 76-77 0.8239544928432458 2314 78-79 0.6663283917822298 2314 80-81 0.6813716917602157 2314 82-83 0.7195638199033034 2314 84-85 0.7110491875927778 2314 86-87 0.7512541757154203 2314 88-89 0.6692847031460438 2314 90-91 0.8226100576432067 2314 92-93 0.8424350984321656 2314 94-95 0.959167017541489 2314 96-97 0.9766619014536033 2314 98-99 1.0680300108603582 2314 100 -0.3471678515254091 2315 1 0.16251108699932715 2315 2 0.22958370641263315 2315 3 0.3200174121827004 2315 4 0.13637339211585697 2315 5 0.233481286951843 2315 6 0.3347335898923589 2315 7 0.3765095464299719 2315 8 0.2844345763369205 2315 9 0.4671998981984089 2315 10-11 0.25103702739335887 2315 12-13 0.4908211506355755 2315 14-15 0.3463203882710104 2315 16-17 0.45830649117293376 2315 18-19 0.5074662731689443 2315 20-21 0.4046166606165684 2315 22-23 0.4508840502863407 2315 24-25 0.5321552502332736 2315 26-27 0.5873550668682626 2315 28-29 0.6364592771521771 2315 30-31 0.5922212329104539 2315 32-33 0.6595997263797457 2315 34-35 0.7365507433992491 2315 36-37 0.6106100476643164 2315 38-39 0.6679571539188913 2315 40-41 0.6716403082614804 2315 42-43 0.7085275788810108 2315 44-45 0.7142704770155177 2315 46-47 0.5965029720491302 2315 48-49 -4.523994307815244 2315 50-51 -0.7682591363180578 2315 52-53 0.27716593243783905 2315 54-55 0.18172477040612023 2315 56-57 0.609130895816854 2315 58-59 0.7112168954195539 2315 60-61 0.5983307011696795 2315 62-63 0.3490419102376521 2315 64-65 0.5383538577188105 2315 66-67 -0.11533084316234721 2315 68-69 0.5701507601847098 2315 70-71 0.5704316970237997 2315 72-73 0.6843123189275673 2315 74-75 0.8425602877445186 2315 76-77 0.7584288935981682 2315 78-79 0.7309460453105103 2315 80-81 0.5614872428915199 2315 82-83 0.5838352629636638 2315 84-85 0.6684464118732265 2315 86-87 0.6576698910296237 2315 88-89 0.6084074919882134 2315 90-91 0.7120794210534704 2315 92-93 0.7476786063759278 2315 94-95 0.42470010148497295 2315 96-97 0.5252873332125958 2315 98-99 0.4364509121621829 2315 100 0.5200178793512613 2316 1 0.26283286807301565 2316 2 0.2820903683907119 2316 3 0.17013882856863205 2316 4 -0.030262193583290298 2316 5 0.195269446918644 2316 6 0.18463987787669822 2316 7 0.3076047119334504 2316 8 0.3556054406759017 2316 9 0.5025878299397846 2316 10-11 0.4470461106433916 2316 12-13 0.4001543464132524 2316 14-15 0.5152010233967559 2316 16-17 0.4570364875992823 2316 18-19 0.21184524179901132 2316 20-21 0.5194435712322232 2316 22-23 0.6408762851296927 2316 24-25 0.6190289356200935 2316 26-27 0.6653810389526029 2316 28-29 0.7056794281150545 2316 30-31 0.6373555766040653 2316 32-33 0.602015525346097 2316 34-35 0.5611027575534919 2316 36-37 0.48210329949147734 2316 38-39 0.559343261077295 2316 40-41 0.22913258982545415 2316 42-43 0.1762015673987989 2316 44-45 0.46024606253921974 2316 46-47 0.4360770402398657 2316 48-49 -4.835929349762722 2316 50-51 -0.8985044443289496 2316 52-53 0.2862995110575639 2316 54-55 0.5624186497006463 2316 56-57 0.4789828277046766 2316 58-59 0.3500085706834497 2316 60-61 0.5724290410051296 2316 62-63 0.42942070190147774 2316 64-65 0.42254654268189995 2316 66-67 0.46042389306831666 2316 68-69 0.14547780638548602 2316 70-71 -0.20382916189557676 2316 72-73 -0.09189395963636571 2316 74-75 0.14926637008187527 2316 76-77 0.18751305134373908 2316 78-79 0.30978081032871074 2316 80-81 0.24738553154405096 2316 82-83 0.1146784346411387 2316 84-85 0.24851632498051401 2316 86-87 0.25225482319320136 2316 88-89 0.17154733078874784 2316 90-91 0.18448692472723494 2316 92-93 0.3072252181016957 2316 94-95 0.47558575650225166 2316 96-97 0.5408244467330121 2316 98-99 0.5397798721918399 2316 100 -0.14997241420293506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 9.0 11 21.0 12 52.0 13 242.0 14 1080.0 15 3141.0 16 7904.0 17 16697.0 18 29827.0 19 44206.0 20 63633.0 21 90108.0 22 105136.0 23 97786.0 24 90008.0 25 92747.0 26 107841.0 27 132683.0 28 163472.0 29 199954.0 30 246593.0 31 305082.0 32 384530.0 33 501568.0 34 693899.0 35 951884.0 36 1430171.0 37 2026427.0 38 1682159.0 39 228926.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.10956622497806 22.399416367436018 15.168402398686103 22.322615008899817 2 14.482485606783682 21.330771331204428 42.04574233853571 22.141000723476186 3 17.861517563841588 27.908793268736503 30.695551039293168 23.53413812812874 4 11.498865612319232 18.231766176176485 40.75372189209904 29.515646319405242 5 11.859128438806035 39.53566644702855 35.50963778270674 13.095567331458676 6 27.934996776587173 40.083550942915274 18.460060409602345 13.521391870895203 7 25.483380101658078 33.746045367182646 23.51267601538204 17.257898515777235 8 25.147642088559884 36.433457991688115 20.474345904557474 17.944554015194527 9 26.019708794293532 15.802284220300578 22.252471643187413 35.925535342218474 10-11 24.23453437479586 27.716052714927446 28.10150975356704 19.94790315670965 12-13 24.74221079580183 25.535566194282772 28.82095135519173 20.901271654723665 14-15 22.254756546644845 26.566088445699805 27.093033135790083 24.086121871865267 16-17 20.188483605993195 29.46585690632993 28.33544713620399 22.010212351472887 18-19 19.766721426483695 28.499244339272874 31.453583128016245 20.280451106227186 20-21 21.303647622238326 27.54787504832283 31.05301131793128 20.09546601150756 22-23 21.263048667367894 27.560530209924817 30.65020592596358 20.526215196743706 24-25 20.623905293178804 27.78123847032554 31.428616505059598 20.16623973143605 26-27 20.49718294311848 27.994054526911444 30.85458519271152 20.654177337258552 28-29 19.990213156442145 28.053114172382703 31.286046514265784 20.670626156909368 30-31 20.73978830546824 27.757859246626833 31.141938001298886 20.360414446606043 32-33 20.93171433618714 28.117483719226016 30.54144189045802 20.409360054128822 34-35 20.880301836042054 28.000459983854153 31.094707426805456 20.024530753298333 36-37 20.737894354253484 27.722081404132375 30.847871700968387 20.692152540645754 38-39 20.752694704096434 27.869700386054667 31.200660865034337 20.17694404481456 40-41 20.59787709254095 27.356463268718162 31.54631966366932 20.499339975071567 42-43 20.475812364459838 27.449174049298207 30.95376449757716 21.121249088664797 44-45 20.57193880476405 28.203698600922927 30.623739553946987 20.600623040366038 46-47 20.655926260156242 27.929077201594012 30.154497653295824 21.260498884953925 48-49 21.261876433578635 27.913754364506328 29.966740479868797 20.857628722046247 50-51 21.08139929252893 27.90216216637116 29.542762385913367 21.473676155186546 52-53 20.50978371112088 28.48321485509406 29.48877755333274 21.518223880452318 54-55 20.78460207344829 28.427779422062127 29.29599077642419 21.491627728065392 56-57 21.15632866422538 28.110681886384235 29.092375325742726 21.64061412364766 58-59 20.525358900029804 28.284751247552077 29.714849659533527 21.475040192884595 60-61 20.87718769069424 28.815579804579578 29.03791494485964 21.26931755986655 62-63 20.94672407700804 28.28740968361493 29.077919910315376 21.68794632906166 64-65 20.33485457920792 28.24582301980198 29.21442347359736 22.204898927392737 66-67 20.77217212243724 28.048284068539992 29.022467724606205 22.157076084416563 68-69 20.498249620424463 28.160837912994687 29.09627500247549 22.244637464105356 70-71 20.604840306720675 27.714660946252923 29.366212785198986 22.31428596182742 72-73 20.929174900407386 28.057308537197223 29.147047634430113 21.866468927965283 74-75 20.92588353943053 28.280062904500152 28.905235543053536 21.888818013015783 76-77 20.625314128475924 28.244509174755905 28.91048345890394 22.21969323786423 78-79 20.533196810552003 28.10801978356588 29.008970109791765 22.34981329609035 80-81 20.286851996399708 28.51656932329622 29.009585880219475 22.1869928000846 82-83 20.969010353808272 27.93991077484248 29.521005069829204 21.570073801520046 84-85 21.057067153653794 28.132995466417604 28.694243591649705 22.1156937882789 86-87 20.23612890190345 28.59074801329443 29.103535389347567 22.069587695454548 88-89 20.844940614490074 27.8519769296745 28.96499687200384 22.338085583831585 90-91 20.684111224773368 27.838531821687763 29.252787227811567 22.224569725727306 92-93 20.813271202605794 28.089528599055498 28.911424524036605 22.185775674302104 94-95 19.999045083997547 27.910689160610268 29.2790858545797 22.811179900812483 96-97 20.341329726064238 27.91288251269199 29.041807459605902 22.70398030163787 98-99 20.02301598629746 28.14881319727599 28.95493478752316 22.873236028903385 100 20.237074025340103 27.920142400382243 28.646647595776347 23.196135978501307 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4060.0 1 2769.5 2 2070.5 3 2507.5 4 1904.0 5 1705.5 6 2763.5 7 5589.0 8 8557.0 9 9755.5 10 8802.5 11 7467.5 12 7778.5 13 8926.0 14 10434.5 15 12255.5 16 14066.0 17 15756.0 18 17650.5 19 19479.0 20 21800.5 21 24509.5 22 27661.5 23 32421.5 24 37971.5 25 45338.0 26 57048.5 27 69904.0 28 81712.0 29 101544.0 30 130979.0 31 159681.5 32 197757.0 33 243115.0 34 282598.0 35 319756.5 36 361313.0 37 408438.0 38 418585.5 39 395462.0 40 367843.0 41 337903.5 42 321493.0 43 321122.0 44 329778.0 45 359192.5 46 379937.5 47 379382.0 48 377640.0 49 364339.5 50 343962.5 51 326827.5 52 307179.5 53 280948.5 54 258324.0 55 224212.0 56 190519.0 57 153440.5 58 117381.0 59 102971.0 60 81964.0 61 55912.5 62 39841.5 63 31192.5 64 21855.5 65 11583.0 66 7157.0 67 5381.5 68 4300.5 69 3541.0 70 2866.5 71 2113.5 72 1608.5 73 1258.5 74 985.0 75 686.0 76 440.0 77 296.5 78 204.0 79 128.5 80 83.5 81 52.5 82 30.5 83 17.5 84 11.0 85 9.5 86 6.5 87 4.0 88 3.0 89 3.0 90 2.5 91 0.5 92 1.0 93 1.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027470133705513773 2 0.0018664017269887359 3 1.2373934101582782E-4 4 0.02336611222848882 5 3.609064112961644E-4 6 9.280450576187086E-5 7 2.0623223502637966E-5 8 0.003784361512734067 9 0.010486909151091408 10-11 9.950705340022819E-4 12-13 7.733708813489238E-5 14-15 1.3405095276714679E-4 16-17 3.969970524257809E-4 18-19 5.1558058756594915E-6 20-21 8.249289401055186E-5 22-23 2.4232287615599612E-4 24-25 3.0934835253956954E-5 26-27 6.702547638357339E-5 28-29 9.486682811213464E-4 30-31 0.002546968102575789 32-33 0.009672291822737206 34-35 0.01868979629926566 36-37 0.021458464054494805 38-39 0.0209119486316749 40-41 0.0045783556175856294 42-43 0.005104247816902897 44-45 0.008672065482859266 46-47 0.01155416096735292 48-49 1.2120320010559091 50-51 0.017730816406392996 52-53 0.0184680966466123 54-55 0.00943512475245687 56-57 0.0075790346372194535 58-59 0.008403963577324971 60-61 0.005233142963794384 62-63 0.007357334984566095 64-65 0.018612459211130764 66-67 0.02850645068652133 68-69 0.028511606492396993 70-71 0.025459369414006568 72-73 0.022494781035502363 74-75 0.009110308982290323 76-77 0.013760845882135185 78-79 0.007903850407386002 80-81 0.002686174861218595 82-83 0.013657729764621993 84-85 0.023422826093121074 86-87 0.022360730082735217 88-89 0.03196599642908885 90-91 0.019855008427164703 92-93 0.013002942418413238 94-95 0.006362264450563813 96-97 0.0035987525012103256 98-99 0.015477729238729795 100 0.03556474893029917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9697805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.55022114676482 #Duplication Level Percentage of deduplicated Percentage of total 1 69.75170788418826 36.65467674678632 2 16.862679935953235 17.72275119522913 3 6.321259038203585 9.965506811507527 4 2.9319785681710577 6.163044886198557 5 1.5026988518601332 3.9483578491119795 6 0.8543297614891889 2.693713073911183 7 0.5214566315229425 1.9181862913484375 8 0.3208486233743991 1.3488532890367781 9 0.2106508532537034 0.9962774030913134 >10 0.6374410515596179 5.271377373640478 >50 0.034939170763208724 1.2885863012272423 >100 0.04007908425951996 4.573457420554421 >500 0.006427470971715842 2.3488527672010515 >1k 0.003320305329031657 2.9135742770416377 >5k 9.138455027189301E-5 0.3439071585325732 >10k+ 9.138455027189301E-5 1.8488771555814656 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47434 0.4891209918120647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40717 0.41985789567845505 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27349 0.28201226978682287 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 1.0311611751318985E-5 0.0 0.0 0.0 0.0 4 1.0311611751318985E-5 0.0 0.0 0.0 0.0 5 1.0311611751318985E-5 0.0 0.0 0.0 0.0 6 1.0311611751318985E-5 0.0 0.0 0.0 0.0 7 1.0311611751318985E-5 0.0 0.0 1.0311611751318985E-5 0.0 8 2.062322350263797E-5 0.0 0.0 1.0311611751318985E-5 0.0 9 2.062322350263797E-5 0.0 0.0 1.0311611751318985E-5 0.0 10-11 3.0934835253956954E-5 0.0 0.0 1.5467417626978477E-5 0.0 12-13 4.124644700527594E-5 0.0 0.0 4.124644700527594E-5 0.0 14-15 4.124644700527594E-5 0.0 0.0 7.218128225923289E-5 0.0 16-17 7.733708813489239E-5 0.0 0.0 9.280450576187086E-5 0.0 18-19 1.0827192338884933E-4 0.0 0.0 1.0311611751318985E-4 0.0 20-21 1.1342772926450883E-4 0.0 0.0 1.8045320564808224E-4 0.0 22-23 1.1858353514016833E-4 0.0 0.0 4.6917833468501375E-4 0.0 24-25 1.2373934101582782E-4 0.0 0.0 9.899147281266225E-4 0.0 26-27 1.392067586428063E-4 0.0 0.0 0.002196373303030944 0.0 28-29 1.5467417626978476E-4 0.0 0.0 0.005516712286955657 0.0 30-31 1.5467417626978476E-4 0.0 0.0 0.01764316770650678 0.0 32-33 1.7529739977242272E-4 0.0 0.0 0.045773244564104976 0.0 34-35 2.0107642915072018E-4 0.0 0.0 0.08892218393749926 0.0 36-37 2.1654384677769866E-4 0.0 0.0 0.159788735698439 0.0 38-39 2.1654384677769866E-4 0.0 0.0 0.25673850938434006 0.0 40-41 2.1654384677769866E-4 0.0 0.0 0.36027740297933397 0.0 42-43 2.3716707028033663E-4 0.0 0.0 0.46518774093725335 0.0 44-45 2.3716707028033663E-4 0.0 0.0 0.5924278741426539 0.0 46-47 2.4232287615599612E-4 0.0 0.0 0.7437043743403791 0.0 48-49 2.526344879073151E-4 0.0 0.0 0.9128044954502591 0.0 50-51 2.577902937829746E-4 0.0 0.0 1.0938145281329126 0.0 52-53 2.629460996586341E-4 0.0 0.0 1.2693542507814912 0.0 54-55 2.887251290369315E-4 0.0 0.0 1.4596137992050777 0.0 56-57 3.093483525395695E-4 0.0 0.0 1.681055661564653 0.0 58-59 3.402831877935265E-4 0.0 0.0 1.9354328118579411 0.0 60-61 3.402831877935265E-4 0.0 0.0 2.2009722818720316 0.0 62-63 3.5059479954484545E-4 0.0 0.0 2.4653980978169803 0.0 64-65 3.609064112961644E-4 0.0 0.0 2.735706688266056 0.0 66-67 3.6606221717182393E-4 0.0 0.0 3.033949434949455 0.0 68-69 3.815296347988024E-4 0.0 0.0 3.3637818042330196 0.0 70-71 3.815296347988024E-4 0.0 0.0 3.7258482718512074 0.0 72-73 3.969970524257809E-4 0.0 0.0 4.085274966861058 0.0 74-75 4.124644700527594E-4 0.0 0.0 4.429074414261784 5.155805875659492E-6 76-77 4.124644700527594E-4 0.0 0.0 4.81360472808022 1.0311611751318985E-5 78-79 4.1762027592841884E-4 0.0 0.0 5.22415639415311 1.0311611751318985E-5 80-81 4.3824349943105684E-4 0.0 0.0 5.671505046760581 1.0311611751318985E-5 82-83 4.7433414056067326E-4 0.0 0.0 6.125282989295 1.0311611751318985E-5 84-85 4.8464575231199224E-4 0.0 0.0 6.5570147059050985 1.0311611751318985E-5 86-87 5.104247816902897E-4 0.0 0.0 7.007874462313895 1.0311611751318985E-5 88 5.362038110685871E-4 0.0 0.0 7.358634247646761 1.0311611751318985E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22040 0.0 57.06391 1 GTATCAA 41025 0.0 41.924095 1 ATCAACG 52095 0.0 32.16511 3 CAACGCA 52860 0.0 31.648863 5 TATCAAC 54825 0.0 31.132402 2 AACGCAG 56790 0.0 29.863476 6 TCAACGC 57445 0.0 29.267817 4 GTGGTAT 8215 0.0 28.791302 1 GTACTAG 5820 0.0 27.17348 1 ACGCAGA 63685 0.0 26.269276 7 CGCAGAG 63945 0.0 26.169802 8 TGGTATC 8995 0.0 26.07939 2 GTACATG 53505 0.0 24.795322 1 TAGGCAT 7515 0.0 24.721231 5 GCTATTG 9720 0.0 24.567186 8 CTGTGCG 6595 0.0 24.39875 9 GCAGAGT 68400 0.0 24.17638 9 TACATGG 54430 0.0 24.057474 2 ACATGGG 53005 0.0 23.849102 3 GAACCGT 965 0.0 23.335539 6 >>END_MODULE