FastQCFastQC Report
Mon 6 Jun 2016
ERR1211271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5997439
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196520.32767319517547405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155610.2594607464952957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104370.17402427936324152No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG43550.058.1404652
ACCTGGG50000.049.8311463
GGTATCA94600.049.2040021
GTATCAA169900.041.385911
CCTGGGG50250.040.508224
GTACCTG66800.039.3145261
GTACTAG31650.037.0272031
GTACATG256600.035.160961
ATCAACG201350.033.737373
CAACGCA201250.033.6840555
CTGGGGG43150.033.4466485
TACATGG270200.033.198772
TATCAAC211600.032.8288082
TGGGGGG184650.032.6891376
ACATGGG278050.031.3459783
AACGCAG224950.030.7198496
ATGGGGG183400.030.4773715
GTGGTAT31800.030.3404521
TCAACGC225400.030.3250754
GCATCGT35600.029.9728038
Produced by FastQC (version 0.11.5)