FastQCFastQC Report
Mon 6 Jun 2016
ERR1211271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5997439
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180270.30057829683636633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155060.2585436883976644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104960.17500803259524608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG42700.057.3475042
GGTATCA96000.050.5481341
ACCTGGG47300.050.484073
CCTGGGG48700.039.57374
GTATCAA163650.039.220521
GTACCTG68150.036.913451
GTACTAG30600.036.8796271
CTGGGGG36250.035.7761425
GTACATG255750.033.4068831
TGGGGGG173750.032.5607266
TACATGG266650.031.7451082
CAACGCA204700.030.4151845
ATCAACG205650.030.3086513
ATGGGGG179100.030.013935
TATCACG3450.029.9714852
ACATGGG273300.029.840883
TATCAAC217650.029.498282
GTGGTAT32450.027.9665761
AACGCAG230100.027.935856
CATGGGG239100.027.8181744
Produced by FastQC (version 0.11.5)