FastQCFastQC Report
Mon 6 Jun 2016
ERR1211270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7306857
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302460.41393994709353144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231920.3174004910729743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154080.21087041938825404No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC91630.12540275524757089No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG37650.041.3293043
GGTATCA178950.038.793171
TACCTGG40850.038.7122272
GTACTAG80550.038.0957951
GTATCAA300600.037.874061
GCATCGT77900.037.3534248
GGGCACC84100.036.93819494
TAGGCAT95700.035.515545
CTAGGCA107500.032.447974
CTGTGCG108350.032.1055459
CAACGCA343600.031.4131015
TAGTACT112700.031.4096534
ATCAACG347950.031.0476173
CCTGGGG41500.030.6982274
GTACATG403550.030.6611351
TACCCCG4000.030.556635
ACTGTGC116150.030.5161238
CTGGGGG39650.030.352115
TATCAAC378950.029.8450812
TACATGG409900.029.7387542
Produced by FastQC (version 0.11.5)