##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211270_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7306857 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.460393299061415 31.0 31.0 34.0 30.0 34.0 2 31.469591508359887 31.0 31.0 34.0 28.0 34.0 3 31.531052681063827 31.0 31.0 34.0 28.0 34.0 4 35.139769534288135 37.0 35.0 37.0 32.0 37.0 5 35.20433751474813 37.0 35.0 37.0 32.0 37.0 6 35.27082027197193 37.0 35.0 37.0 32.0 37.0 7 35.1839966212559 37.0 35.0 37.0 32.0 37.0 8 35.199184546789404 37.0 35.0 37.0 32.0 37.0 9 36.75097747225654 39.0 37.0 39.0 32.0 39.0 10-11 36.70058631228174 39.0 37.0 39.0 32.0 39.0 12-13 36.568018506452226 39.0 37.0 39.0 32.0 39.0 14-15 37.7193795499214 40.0 37.0 41.0 32.0 41.0 16-17 37.70831507993108 40.0 37.0 41.0 32.0 41.0 18-19 37.73919497808703 40.0 37.0 41.0 32.0 41.0 20-21 37.712473707915734 40.0 37.0 41.0 32.0 41.0 22-23 37.59021293286567 40.0 37.0 41.0 32.0 41.0 24-25 37.47930964024614 40.0 37.0 41.0 31.5 41.0 26-27 37.31749718107252 40.0 36.0 41.0 31.0 41.0 28-29 37.1986237721636 40.0 36.0 41.0 31.0 41.0 30-31 37.03498535690517 39.0 36.0 41.0 30.5 41.0 32-33 36.840974785738936 39.0 35.0 41.0 30.0 41.0 34-35 36.68567326553674 39.0 35.0 41.0 30.0 41.0 36-37 36.57499962021974 39.0 35.0 41.0 30.0 41.0 38-39 36.41154965808144 39.0 35.0 40.5 30.0 41.0 40-41 36.228741167371965 39.0 35.0 40.0 29.0 41.0 42-43 36.00044485611255 38.0 35.0 40.0 28.0 41.0 44-45 35.72218089118208 38.0 34.5 40.0 27.0 41.0 46-47 35.43466301584936 38.0 34.0 40.0 26.0 41.0 48-49 35.17282738665886 38.0 34.0 40.0 26.0 41.0 50-51 34.44871598007187 37.5 33.0 39.5 24.5 40.5 52-53 34.53230568218319 37.5 33.0 39.5 25.0 40.5 54-55 34.976414619856385 38.0 34.0 40.0 26.0 41.0 56-57 34.89101408444151 37.0 34.0 40.0 26.0 41.0 58-59 34.632702268567726 37.0 33.5 40.0 26.0 41.0 60-61 34.39196675123107 36.0 33.0 40.0 25.5 41.0 62-63 34.069404875447816 36.0 33.0 39.0 25.0 41.0 64-65 33.81694331776303 35.0 33.0 39.0 25.0 41.0 66-67 33.652807698303114 35.0 33.0 39.0 24.5 41.0 68-69 33.36962397101791 35.0 33.0 38.5 23.5 41.0 70-71 33.12085271410129 35.0 32.0 37.5 24.0 40.0 72-73 32.83183309869072 35.0 32.0 37.0 23.0 40.0 74-75 32.52734342549745 35.0 32.0 36.5 23.0 39.5 76-77 32.24302186288852 35.0 31.5 36.0 22.5 39.0 78-79 31.93547944074997 35.0 31.0 36.0 21.0 39.0 80-81 31.68102995583464 35.0 31.0 35.0 20.0 37.5 82-83 31.409819023418688 35.0 31.0 35.0 20.0 37.0 84-85 31.18783794454989 34.0 31.0 35.0 20.0 37.0 86-87 30.982560080209588 34.0 30.0 35.0 19.0 36.0 88-89 30.79977923476537 34.0 30.0 35.0 19.0 36.0 90-91 30.572464261993908 34.0 30.0 35.0 18.5 36.0 92-93 30.36546732747062 34.0 29.5 35.0 18.0 35.0 94-95 30.214324845826326 34.0 29.0 35.0 18.0 35.0 96-97 30.027297304436093 34.0 29.0 35.0 17.0 35.0 98-99 29.79302934216449 34.0 29.0 35.0 15.5 35.0 100 28.62676880086746 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4370954025351246 1101 2 -0.3987971189083801 1101 3 -0.4546948782819733 1101 4 -0.3159425248318328 1101 5 -0.34353299580824626 1101 6 -0.31404441275766715 1101 7 -0.2960018923881478 1101 8 -0.32599420096259024 1101 9 -0.4454875281198767 1101 10-11 -0.4568116651331806 1101 12-13 -0.4976061935377274 1101 14-15 -0.6486527494293952 1101 16-17 -0.6500413709793662 1101 18-19 -0.5776655425852653 1101 20-21 -0.68029537088875 1101 22-23 -0.6969908702293992 1101 24-25 -0.8078101151835071 1101 26-27 -0.867747816506828 1101 28-29 -0.8315948082601494 1101 30-31 -0.8446622121646357 1101 32-33 -0.8874626382582846 1101 34-35 -0.9169277298267033 1101 36-37 -0.8544224589294558 1101 38-39 -0.814779779529573 1101 40-41 -0.9165763163290492 1101 42-43 -0.9253658348190754 1101 44-45 -0.9177450038437343 1101 46-47 -1.0279857916183843 1101 48-49 -1.0948411185036164 1101 50-51 -1.0968058027968652 1101 52-53 -0.937164400300766 1101 54-55 -0.7819564631893101 1101 56-57 -0.6216217550779746 1101 58-59 -0.6939791592494728 1101 60-61 -0.7281768301880192 1101 62-63 -0.7441986038827295 1101 64-65 -0.7142596293450794 1101 66-67 -0.7724147246142081 1101 68-69 -0.660191845764146 1101 70-71 -0.8301957588749715 1101 72-73 -0.7914528756068151 1101 74-75 -0.8591482673844766 1101 76-77 -0.8460240478904169 1101 78-79 -0.9196390407253787 1101 80-81 -0.9699541431145242 1101 82-83 -0.9474249525375598 1101 84-85 -1.0077926853319674 1101 86-87 -1.0919328923770202 1101 88-89 -1.1106704566078136 1101 90-91 -1.184789027317592 1101 92-93 -1.343339056750299 1101 94-95 -1.399030521841052 1101 96-97 -1.463780794501897 1101 98-99 -1.4349614393230326 1101 100 -1.5815382167893368 1102 1 -0.4514818242636913 1102 2 -0.4186552860161825 1102 3 -0.4249699296486007 1102 4 -0.2688340084607219 1102 5 -0.37537128503546313 1102 6 -0.4226416835196787 1102 7 -0.37695296353669505 1102 8 -0.44878982501689535 1102 9 -0.5819893194426697 1102 10-11 -0.5757369384066138 1102 12-13 -0.5903809575711207 1102 14-15 -0.6888532959390758 1102 16-17 -0.74295898798443 1102 18-19 -0.7741674882683043 1102 20-21 -0.8446829689168069 1102 22-23 -0.7603885773659727 1102 24-25 -0.7755727442695886 1102 26-27 -0.8152105152596008 1102 28-29 -0.8864004845142617 1102 30-31 -0.8872846337905429 1102 32-33 -0.9092927343038397 1102 34-35 -0.9280643801200199 1102 36-37 -0.9384629900254211 1102 38-39 -0.9995592266297564 1102 40-41 -1.0093953659460126 1102 42-43 -0.8625677382111334 1102 44-45 -1.1015634189098904 1102 46-47 -1.0533777271694476 1102 48-49 -1.1683597204014688 1102 50-51 -1.1443554779708265 1102 52-53 -1.0533208598494497 1102 54-55 -0.8436221274442417 1102 56-57 -0.8139243580753188 1102 58-59 -0.8089884943250283 1102 60-61 -0.7765976411484132 1102 62-63 -0.8725906703610349 1102 64-65 -0.9069878853792659 1102 66-67 -0.8943554393058832 1102 68-69 -0.9378932803101918 1102 70-71 -0.9231025195191265 1102 72-73 -0.984477414980951 1102 74-75 -1.0130628479560642 1102 76-77 -1.0917800476687347 1102 78-79 -1.0101389903258244 1102 80-81 -1.109231815257818 1102 82-83 -1.1069079163733733 1102 84-85 -1.105793938625606 1102 86-87 -1.2497492304609317 1102 88-89 -1.2999505510911646 1102 90-91 -1.3675613494834273 1102 92-93 -1.3157197074512794 1102 94-95 -1.3347965959503334 1102 96-97 -1.311462633665208 1102 98-99 -1.5163563194353316 1102 100 -1.5952809283819036 1103 1 -0.46051480934704614 1103 2 -0.43306070307568234 1103 3 -0.549078475476545 1103 4 -0.369207472762362 1103 5 -0.42471306333945336 1103 6 -0.388433340137162 1103 7 -0.41061088558424075 1103 8 -0.40208193841557005 1103 9 -0.6185490810394327 1103 10-11 -0.594604376850782 1103 12-13 -0.582936863021061 1103 14-15 -0.7168029376399048 1103 16-17 -0.8153089262276154 1103 18-19 -0.6898579678520136 1103 20-21 -0.6513494848456887 1103 22-23 -0.7250087729414076 1103 24-25 -0.786226640247726 1103 26-27 -0.878033139814697 1103 28-29 -0.8723219172358725 1103 30-31 -0.8860475104523076 1103 32-33 -0.9374834651172037 1103 34-35 -0.9609568510870119 1103 36-37 -0.9176592789523212 1103 38-39 -0.8938401434078784 1103 40-41 -0.9192397314586671 1103 42-43 -0.9833932377470447 1103 44-45 -1.0507115219889656 1103 46-47 -1.1388746046132283 1103 48-49 -1.128019888898912 1103 50-51 -1.2242763132226386 1103 52-53 -1.0854813617971644 1103 54-55 -0.8490388671958939 1103 56-57 -0.7206677731176754 1103 58-59 -0.8479793139366549 1103 60-61 -0.7915863221105042 1103 62-63 -0.799354439573257 1103 64-65 -0.7215065841740937 1103 66-67 -0.9153977271949714 1103 68-69 -0.916595126437052 1103 70-71 -0.850781198617554 1103 72-73 -0.8466314717460612 1103 74-75 -0.8420291018752479 1103 76-77 -0.9554014226532033 1103 78-79 -0.8976184580112943 1103 80-81 -0.9899677993837557 1103 82-83 -0.9692324210398482 1103 84-85 -1.0761676358856498 1103 86-87 -0.9757820127334362 1103 88-89 -1.0723902239992924 1103 90-91 -1.1342948203992549 1103 92-93 -1.2407343016103276 1103 94-95 -1.285665782039338 1103 96-97 -1.3258361445585294 1103 98-99 -1.3970183735255333 1103 100 -1.384109704866077 1104 1 -0.5132962124675977 1104 2 -0.5141488327751418 1104 3 -0.5529532016941694 1104 4 -0.4595204314183974 1104 5 -0.4287898804641159 1104 6 -0.4201566449444414 1104 7 -0.480850685073932 1104 8 -0.44906886460158546 1104 9 -0.605612327590336 1104 10-11 -0.5837620303382636 1104 12-13 -0.5438096925195595 1104 14-15 -0.7692927201946063 1104 16-17 -0.7861816428261719 1104 18-19 -0.8514354059263951 1104 20-21 -0.7629453256115966 1104 22-23 -0.7346039137276463 1104 24-25 -0.7667018361291298 1104 26-27 -0.8010572778235954 1104 28-29 -0.8290285128508472 1104 30-31 -0.8820125792083786 1104 32-33 -0.9495603725618267 1104 34-35 -1.0208696923271035 1104 36-37 -0.9711916925274053 1104 38-39 -0.9318517495225436 1104 40-41 -0.9963738791769501 1104 42-43 -0.9796370564748855 1104 44-45 -1.0435352625900407 1104 46-47 -0.9991963694652171 1104 48-49 -0.9627852828068271 1104 50-51 -1.0258157040145264 1104 52-53 -0.8198757664757395 1104 54-55 -0.6717228376612709 1104 56-57 -0.6895360640382577 1104 58-59 -0.7326832902728242 1104 60-61 -0.8140313962857633 1104 62-63 -0.7564920871899332 1104 64-65 -0.7294653531130564 1104 66-67 -0.6692189388372185 1104 68-69 -0.860348936810297 1104 70-71 -0.7965176449787847 1104 72-73 -0.7261755817557685 1104 74-75 -0.7706376100153314 1104 76-77 -0.7411421592124654 1104 78-79 -0.752503751664058 1104 80-81 -0.8749937663718121 1104 82-83 -0.9207849082257056 1104 84-85 -0.9193128026388742 1104 86-87 -0.8495165172155623 1104 88-89 -0.9048461365695672 1104 90-91 -0.9986143724122627 1104 92-93 -1.099511482272824 1104 94-95 -1.0069432449517066 1104 96-97 -1.1443871866250497 1104 98-99 -1.1702540860371045 1104 100 -1.0671783254545133 1105 1 -0.6408783242799032 1105 2 -0.6136930579579527 1105 3 -0.6765310618341864 1105 4 -0.44105136734280137 1105 5 -0.39099841274677516 1105 6 -0.4044941297797706 1105 7 -0.479394765566326 1105 8 -0.5004449817785641 1105 9 -0.6238614622303373 1105 10-11 -0.725856090240093 1105 12-13 -0.745416034411889 1105 14-15 -0.9152779506419222 1105 16-17 -0.8531711917259628 1105 18-19 -0.8315996852011551 1105 20-21 -0.8627684137503238 1105 22-23 -0.8477029598838897 1105 24-25 -0.8851370092567805 1105 26-27 -0.8924251587767671 1105 28-29 -0.8815086735685114 1105 30-31 -0.940963851927556 1105 32-33 -1.0131640543764675 1105 34-35 -0.9829545699523976 1105 36-37 -0.9428575244416564 1105 38-39 -0.9714304778050646 1105 40-41 -0.9771457469076594 1105 42-43 -0.890455447740031 1105 44-45 -1.0887011000693079 1105 46-47 -1.0642450633559406 1105 48-49 -1.1193914577486836 1105 50-51 -1.0424548238515783 1105 52-53 -0.9052751120298481 1105 54-55 -0.7682340734287436 1105 56-57 -0.7072236885554943 1105 58-59 -0.7329144053907513 1105 60-61 -0.7124601139947657 1105 62-63 -0.7773196607316208 1105 64-65 -0.8346862286013916 1105 66-67 -0.7443847206663179 1105 68-69 -0.8137561179070474 1105 70-71 -0.828685330043399 1105 72-73 -0.7584797210147336 1105 74-75 -0.7856065572255915 1105 76-77 -0.7864959272848537 1105 78-79 -0.8642097073442727 1105 80-81 -0.815278973446059 1105 82-83 -0.9818609040853339 1105 84-85 -0.8889431742706471 1105 86-87 -0.9092761085207819 1105 88-89 -0.9795667727622082 1105 90-91 -1.1250635582504103 1105 92-93 -1.1299035376558635 1105 94-95 -1.0921006236065303 1105 96-97 -1.1066344514196444 1105 98-99 -1.0064301408859038 1105 100 -1.1674358743988265 1106 1 -0.5595055706064826 1106 2 -0.5034536292154748 1106 3 -0.5561042597840036 1106 4 -0.3859327360922151 1106 5 -0.4115819062506745 1106 6 -0.37150667297331097 1106 7 -0.3950412638700982 1106 8 -0.40720608343698217 1106 9 -0.5858591568244478 1106 10-11 -0.5317643164429526 1106 12-13 -0.6689088884413579 1106 14-15 -0.7982655059138963 1106 16-17 -0.7201056845891785 1106 18-19 -0.8006482326379114 1106 20-21 -0.7675052285616388 1106 22-23 -0.7551930017382915 1106 24-25 -0.7684039768340583 1106 26-27 -0.8168615749996846 1106 28-29 -0.879645279370358 1106 30-31 -0.9614908918704188 1106 32-33 -1.0086029676848014 1106 34-35 -1.0111829587415073 1106 36-37 -1.0481356698240205 1106 38-39 -1.0333788411769476 1106 40-41 -1.0426398205966123 1106 42-43 -0.9666185223847705 1106 44-45 -1.0269297776905901 1106 46-47 -0.9752548534537269 1106 48-49 -1.0109998000095501 1106 50-51 -1.0266688327371085 1106 52-53 -1.0287197507795725 1106 54-55 -0.8901356591173695 1106 56-57 -0.8225049782699188 1106 58-59 -0.8584097197451896 1106 60-61 -0.8566044027324011 1106 62-63 -0.8275240751375179 1106 64-65 -0.8302045707572319 1106 66-67 -0.924220165100266 1106 68-69 -0.8830319565706475 1106 70-71 -0.8626820255407708 1106 72-73 -0.8466718679305316 1106 74-75 -0.8338138940879247 1106 76-77 -0.8065251912354476 1106 78-79 -0.863247830565328 1106 80-81 -0.8823742933145624 1106 82-83 -0.8179001681934714 1106 84-85 -0.9240402218806913 1106 86-87 -1.02100463122299 1106 88-89 -1.0449346464847835 1106 90-91 -1.125203215170302 1106 92-93 -1.140380990366161 1106 94-95 -1.268774406047637 1106 96-97 -1.2052345343932487 1106 98-99 -1.1122878871022017 1106 100 -1.2355561546185498 1107 1 -0.5420946901306252 1107 2 -0.5827893722341493 1107 3 -0.5945088291473191 1107 4 -0.4650097844251846 1107 5 -0.4261019510297146 1107 6 -0.4227991086101355 1107 7 -0.37746433672440816 1107 8 -0.41098506795447065 1107 9 -0.557622016737298 1107 10-11 -0.562943135650265 1107 12-13 -0.6593517057846654 1107 14-15 -0.8568929109124426 1107 16-17 -0.7878774989704382 1107 18-19 -0.8195177125904465 1107 20-21 -0.8135987044674593 1107 22-23 -0.8760665662380518 1107 24-25 -0.8360727652825659 1107 26-27 -0.9012270507501725 1107 28-29 -0.9581042976462086 1107 30-31 -0.8407605677312588 1107 32-33 -0.9251410402445188 1107 34-35 -1.0304840847120076 1107 36-37 -1.0972913425942394 1107 38-39 -1.0362475401075457 1107 40-41 -0.9593520998817624 1107 42-43 -0.941506474359791 1107 44-45 -0.9443517307342049 1107 46-47 -0.8311349225639546 1107 48-49 -0.8247320520978576 1107 50-51 -0.9272952571898898 1107 52-53 -0.8438493810043539 1107 54-55 -0.7972403726333042 1107 56-57 -0.7540911976684512 1107 58-59 -0.7685784295223854 1107 60-61 -0.75617173566134 1107 62-63 -0.6800075106431152 1107 64-65 -0.697715857578757 1107 66-67 -0.6443988862195269 1107 68-69 -0.6063183197870288 1107 70-71 -0.7367489754910892 1107 72-73 -0.7001763565469616 1107 74-75 -0.6795206335740929 1107 76-77 -0.685502360487888 1107 78-79 -0.6852587729069626 1107 80-81 -0.6865893679418242 1107 82-83 -0.6429338043611139 1107 84-85 -0.6271551368888986 1107 86-87 -0.7412357022374003 1107 88-89 -0.7860806513885166 1107 90-91 -0.8147335625426138 1107 92-93 -0.8500238661605728 1107 94-95 -0.9325658875856568 1107 96-97 -0.9184684205013056 1107 98-99 -0.8951217329707184 1107 100 -0.9279821500281642 1108 1 -0.5507715210963227 1108 2 -0.4837832223117111 1108 3 -0.5004911483766925 1108 4 -0.29141245268699834 1108 5 -0.3212931701425745 1108 6 -0.3056063058875296 1108 7 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42-43 1.344595940239607 2310 44-45 1.4508462937351752 2310 46-47 1.4268848034918449 2310 48-49 1.4186751647763813 2310 50-51 1.3898987418814457 2310 52-53 1.329158248994247 2310 54-55 1.1451669502225812 2310 56-57 1.1859337488339392 2310 58-59 1.1669071963591122 2310 60-61 1.1208544831650613 2310 62-63 1.1215901287278527 2310 64-65 1.140614168192883 2310 66-67 1.1032744492189295 2310 68-69 1.0521857247258097 2310 70-71 1.0716131059885257 2310 72-73 1.1153497750044323 2310 74-75 1.0768722945519826 2310 76-77 1.0826578130227134 2310 78-79 1.108311946852659 2310 80-81 1.0728273534049286 2310 82-83 1.0885393208554603 2310 84-85 1.095006585395911 2310 86-87 1.1131100352118999 2310 88-89 1.1637885993620287 2310 90-91 1.292388258126909 2310 92-93 1.297508764691731 2310 94-95 1.3357193483541216 2310 96-97 1.2941103803931853 2310 98-99 1.364699051922038 2310 100 1.441419444104561 2311 1 0.7619867081485552 2311 2 0.7547538250812629 2311 3 0.8196832623786783 2311 4 0.6833077895700157 2311 5 0.6515485265109149 2311 6 0.6939315421258669 2311 7 0.6359173840570733 2311 8 0.6167555493963164 2311 9 0.885122259727666 2311 10-11 0.8793601240946671 2311 12-13 0.9326020957808012 2311 14-15 1.1072643107750153 2311 16-17 1.1401449279972198 2311 18-19 1.0767550069315206 2311 20-21 1.121700363904047 2311 22-23 1.129584620264346 2311 24-25 1.1783269450005847 2311 26-27 1.1754865940476051 2311 28-29 1.1911627162933982 2311 30-31 1.2862189646283113 2311 32-33 1.262558136730469 2311 34-35 1.3069727784871432 2311 36-37 1.3285580734939728 2311 38-39 1.3682857876853376 2311 40-41 1.3869013289408727 2311 42-43 1.4283175129692651 2311 44-45 1.435929577284135 2311 46-47 1.4627021999226386 2311 48-49 1.4652270951900306 2311 50-51 1.443833222498597 2311 52-53 1.3251016097267012 2311 54-55 1.2555784564340229 2311 56-57 1.2336154696652173 2311 58-59 1.1956044436178317 2311 60-61 1.129408933887973 2311 62-63 1.1171617792628084 2311 64-65 1.144988896149819 2311 66-67 1.101548722150639 2311 68-69 1.1102369597324468 2311 70-71 1.1311617093105824 2311 72-73 1.1280307205375735 2311 74-75 1.1647972031888045 2311 76-77 1.147446369149968 2311 78-79 1.1762712559006587 2311 80-81 1.176824546589554 2311 82-83 1.2281080386382826 2311 84-85 1.2221297019527348 2311 86-87 1.253312372283201 2311 88-89 1.310221558899336 2311 90-91 1.368777065025725 2311 92-93 1.4206616267225094 2311 94-95 1.4177569105649113 2311 96-97 1.4724715536199753 2311 98-99 1.524928874689671 2311 100 1.5547516991044468 2312 1 0.682799049122913 2312 2 0.6890168617745438 2312 3 0.6939533517252734 2312 4 0.4234331008024341 2312 5 0.5522837948429427 2312 6 0.564858245185782 2312 7 0.44249369103842184 2312 8 0.5650031014242458 2312 9 0.7775519572428777 2312 10-11 0.7511614925431971 2312 12-13 0.9003148735520838 2312 14-15 1.0841708650668096 2312 16-17 1.119085938981442 2312 18-19 1.070344008688842 2312 20-21 1.0773041060964488 2312 22-23 1.1474500431702737 2312 24-25 1.1392398544733524 2312 26-27 1.1880146000949665 2312 28-29 1.201923662517892 2312 30-31 1.203882062106885 2312 32-33 1.2439117591056927 2312 34-35 1.192022517227251 2312 36-37 1.2127671437516412 2312 38-39 1.3047619353000997 2312 40-41 1.317300018160445 2312 42-43 1.3136676713617277 2312 44-45 1.336892498251558 2312 46-47 1.4330789129186101 2312 48-49 1.4357721367071647 2312 50-51 1.406756235579131 2312 52-53 1.3118450567986741 2312 54-55 1.2595435282658798 2312 56-57 1.171701843237635 2312 58-59 1.1228576884228332 2312 60-61 1.111889324784208 2312 62-63 1.1129053471260946 2312 64-65 1.1310879606017181 2312 66-67 1.1512952552455005 2312 68-69 1.134788862974716 2312 70-71 1.102685160356991 2312 72-73 1.116547461822094 2312 74-75 1.13794042660205 2312 76-77 1.1514973586644999 2312 78-79 1.1602583234385833 2312 80-81 1.1143499225037665 2312 82-83 1.1551712319705985 2312 84-85 1.2433718261651556 2312 86-87 1.2090344704670422 2312 88-89 1.1924784858907387 2312 90-91 1.2417051045829268 2312 92-93 1.3211885300621944 2312 94-95 1.3402233130493144 2312 96-97 1.3274262374000436 2312 98-99 1.0135241303191336 2312 100 1.274461527230116 2313 1 0.6804684586519443 2313 2 0.6197201510350929 2313 3 0.6710562400473954 2313 4 0.5621906265514198 2313 5 0.4749136323585148 2313 6 0.5659649569906406 2313 7 0.6118688663402665 2313 8 0.5678047782339632 2313 9 0.7479426873900081 2313 10-11 0.805258337436122 2313 12-13 0.8969983528887582 2313 14-15 1.0719257729749074 2313 16-17 1.0957273893609667 2313 18-19 1.0894608379921351 2313 20-21 1.0875761691246666 2313 22-23 1.1264743620206872 2313 24-25 1.1053428781448034 2313 26-27 1.0920061687556668 2313 28-29 1.184095301777667 2313 30-31 1.1848417540974694 2313 32-33 1.2057038793828738 2313 34-35 1.2568729480621101 2313 36-37 1.236656531820465 2313 38-39 1.2853848574976112 2313 40-41 1.2903700943257022 2313 42-43 1.270416371138431 2313 44-45 1.3293966592922644 2313 46-47 1.2774342206779963 2313 48-49 1.2602286873973938 2313 50-51 1.2707585723934969 2313 52-53 1.2518209983190616 2313 54-55 1.1109687458575976 2313 56-57 1.0295270458267822 2313 58-59 1.105296493130595 2313 60-61 1.0892943437983433 2313 62-63 1.1533496571271584 2313 64-65 1.1426140976991732 2313 66-67 1.0946094331151812 2313 68-69 1.080427842277686 2313 70-71 1.075905357412573 2313 72-73 1.1033060844985982 2313 74-75 1.042940184712684 2313 76-77 0.8711077102248765 2313 78-79 1.0607212833817101 2313 80-81 1.1874192376687596 2313 82-83 1.2070942388565626 2313 84-85 1.220527807844622 2313 86-87 1.2035138076792968 2313 88-89 1.2623797162107309 2313 90-91 1.2662226304188877 2313 92-93 1.3549517334166836 2313 94-95 1.3866528629067894 2313 96-97 1.4322839466588064 2313 98-99 1.509184906843796 2313 100 1.694170455502583 2314 1 0.648955854486843 2314 2 0.6308167292605553 2314 3 0.6493766043977622 2314 4 0.5235416381070479 2314 5 0.5296453910477723 2314 6 0.5766805426443611 2314 7 0.6048782631135055 2314 8 0.6168907299911837 2314 9 0.7569082742959665 2314 10-11 0.7638991988737942 2314 12-13 0.8539670628694083 2314 14-15 1.0164851447869196 2314 16-17 1.0205658301444274 2314 18-19 0.973719395767759 2314 20-21 1.0242254839850276 2314 22-23 1.080207557023762 2314 24-25 1.0782994493744482 2314 26-27 1.0950472742122415 2314 28-29 1.0931720663023512 2314 30-31 1.1156113027417973 2314 32-33 1.1362454690370711 2314 34-35 1.1301317149610242 2314 36-37 1.1905555436398956 2314 38-39 1.2109226716625585 2314 40-41 1.2819671784542024 2314 42-43 1.317342686695035 2314 44-45 1.3409692434382947 2314 46-47 1.304061333407546 2314 48-49 1.3040707475581215 2314 50-51 1.3087333886905839 2314 52-53 1.288806287894289 2314 54-55 1.1270522465730437 2314 56-57 1.1057973549953104 2314 58-59 1.0818247001467896 2314 60-61 1.03388997825585 2314 62-63 1.0732852838875928 2314 64-65 1.1190686315420848 2314 66-67 1.0951856700314124 2314 68-69 1.1327925633047187 2314 70-71 1.1357576625271975 2314 72-73 1.0974120219302392 2314 74-75 1.13431333787441 2314 76-77 1.115066823074649 2314 78-79 1.1254443139913377 2314 80-81 1.0758776359096167 2314 82-83 1.137376720169037 2314 84-85 1.240051042234679 2314 86-87 1.3105709985149439 2314 88-89 1.3069634126922836 2314 90-91 1.433809376928071 2314 92-93 1.4740033551448022 2314 94-95 1.4976466117484755 2314 96-97 1.4493244681563482 2314 98-99 1.5524138413251762 2314 100 1.6095020306976373 2315 1 0.44481654266825643 2315 2 0.4915003946163097 2315 3 0.45626831001179013 2315 4 0.2075058795860727 2315 5 0.3094339780413762 2315 6 0.3886502088657551 2315 7 0.4047264899640055 2315 8 0.40685027022638565 2315 9 0.6340367060754417 2315 10-11 0.4940287238493539 2315 12-13 0.5393737724849146 2315 14-15 0.7240145593022547 2315 16-17 0.7179618263247178 2315 18-19 0.6189025434024487 2315 20-21 0.7351041373094844 2315 22-23 0.5942467152860047 2315 24-25 0.7135185438110838 2315 26-27 0.8482793986936272 2315 28-29 0.8700275758279332 2315 30-31 0.8947986538215034 2315 32-33 0.8567295939780166 2315 34-35 0.859265235900935 2315 36-37 0.9397062599282364 2315 38-39 0.9963299141730957 2315 40-41 1.0263176330897892 2315 42-43 1.074815320148204 2315 44-45 1.0746055402838977 2315 46-47 0.9855455420633277 2315 48-49 0.99533856057446 2315 50-51 0.9637546753309252 2315 52-53 0.9930528303216661 2315 54-55 0.8801928627844617 2315 56-57 0.801800082282405 2315 58-59 0.7981063542405664 2315 60-61 0.8525139227753087 2315 62-63 0.8289429968683137 2315 64-65 0.8071978412192706 2315 66-67 0.8030452214045027 2315 68-69 0.8894131969589765 2315 70-71 0.7984509085872062 2315 72-73 0.5747069066334376 2315 74-75 0.7800734056679488 2315 76-77 0.8298339972362996 2315 78-79 0.8940884014267994 2315 80-81 0.6061186352996693 2315 82-83 0.5767372980037635 2315 84-85 0.9008937361122094 2315 86-87 1.0382956572530944 2315 88-89 1.0740133375502126 2315 90-91 1.1988022108447431 2315 92-93 1.2336981866771985 2315 94-95 1.2031146773235157 2315 96-97 1.0523142260429097 2315 98-99 0.4993710378028773 2315 100 1.033617275273489 2316 1 0.34562158428388656 2316 2 0.3285653415288259 2316 3 0.30737808398329847 2316 4 0.19290246145276058 2316 5 0.15100157530298475 2316 6 0.22205285265278008 2316 7 0.1868516732394525 2316 8 0.2392618719649917 2316 9 0.2923917890569854 2316 10-11 0.3001654853391855 2316 12-13 0.04609892094173773 2316 14-15 0.054164530848453296 2316 16-17 0.2604179795337629 2316 18-19 0.3186983177360716 2316 20-21 0.3582682010447016 2316 22-23 0.3361624833805905 2316 24-25 0.39922676766378373 2316 26-27 0.4514567068181421 2316 28-29 0.4443931975790676 2316 30-31 0.3240079417058368 2316 32-33 0.4744128658617939 2316 34-35 0.4827835121392212 2316 36-37 0.4383686313469539 2316 38-39 0.5571408846432746 2316 40-41 0.6206923358488083 2316 42-43 0.5801161468123652 2316 44-45 0.5414861123095278 2316 46-47 0.2979738060649879 2316 48-49 0.23755429848768728 2316 50-51 0.3607634614379762 2316 52-53 0.5370310295295582 2316 54-55 0.21877409927687808 2316 56-57 0.037200785513782364 2316 58-59 0.11832125420936279 2316 60-61 0.20925271638640197 2316 62-63 0.3626766077621184 2316 64-65 0.2635850589727653 2316 66-67 0.18174660361192707 2316 68-69 0.15194950496120896 2316 70-71 -0.07395435826373387 2316 72-73 -0.17013195944300463 2316 74-75 0.06774100531564642 2316 76-77 0.23969184928022713 2316 78-79 0.31762369764845744 2316 80-81 0.4747137310131784 2316 82-83 0.49918016596321735 2316 84-85 0.496303540653134 2316 86-87 0.6049717569086823 2316 88-89 0.6021388150968789 2316 90-91 0.4575844663697879 2316 92-93 0.48145368118872867 2316 94-95 0.6957367278285638 2316 96-97 0.6852357796854811 2316 98-99 0.5407688772415256 2316 100 0.4347456850089131 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 29.0 1 438.0 2 163.0 3 148.0 4 141.0 5 104.0 6 120.0 7 461.0 8 1605.0 9 3083.0 10 4927.0 11 7548.0 12 10787.0 13 13671.0 14 16671.0 15 20114.0 16 24889.0 17 31847.0 18 39642.0 19 48568.0 20 60732.0 21 75587.0 22 82762.0 23 81675.0 24 78441.0 25 79971.0 26 87008.0 27 99404.0 28 117387.0 29 141319.0 30 173620.0 31 215689.0 32 273075.0 33 361957.0 34 528495.0 35 720618.0 36 1077740.0 37 1418935.0 38 1082099.0 39 325137.0 40 250.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98142664396645 20.667932040107253 12.562142949769806 22.788498366156496 2 15.805181055182494 19.70129135822974 41.339839320908425 23.15368826567934 3 19.32279658191164 27.242661934591585 28.298317291766708 25.136224191730072 4 12.381738467455921 16.03678259096067 39.48688887885097 32.09459006273244 5 12.818762796197275 38.907913646808595 34.02887428506584 14.244449271928286 6 29.319744732429402 37.989673400901154 18.268656986187477 14.421924880481967 7 27.375319282025735 32.29203995830043 22.93690029992963 17.395740459744207 8 27.13494071619259 34.5432908787707 19.610094616663602 18.71167378837311 9 27.351821992110793 14.960608036719009 21.696049823238294 35.99152014793191 10-11 26.555314910204324 25.893887037612828 26.52497524976482 21.025822802418027 12-13 26.337461095998894 23.758487958996017 27.521469422536747 22.382581522468346 14-15 23.70952664951707 24.747564066992112 25.649841072165053 25.89306821132577 16-17 21.765819697173775 27.422670026292245 26.9372531337707 23.87425714276328 18-19 21.085728427521218 27.482341269216615 29.67924027522475 21.75269002803742 20-21 22.866583704488924 26.638695791857376 28.87874987834237 21.61597062531133 22-23 23.21694973723513 26.35049096442031 28.58948858386231 21.843070714482252 24-25 22.477690939039068 26.18221852766871 29.63865738039349 21.701433152898733 26-27 22.3255720681237 26.49956802771303 28.51780223396369 22.657057670199578 28-29 21.47288465885794 27.159184572423552 29.062582150823452 22.30534861789506 30-31 22.733314411991703 26.661050947525993 28.941371467844178 21.664263172638123 32-33 23.03438173471327 26.829786967546344 27.977120138301455 22.15871115943893 34-35 22.922612922246742 26.72356683276998 28.935733041942424 21.41808720304085 36-37 22.34957402484087 26.40140464720956 28.534418627221957 22.714602700727614 38-39 22.74624514499116 26.618014598084528 28.96218274243697 21.673557514487342 40-41 22.33758468323087 25.458147923266942 29.65071005613003 22.55355733737216 42-43 22.311472236437062 25.82662589221493 28.6984908360159 23.163411035332107 44-45 22.221676848945478 26.809781285691713 28.71603887585013 22.252502989512678 46-47 22.27477804670696 26.573519833295244 27.973440227092645 23.178261892905148 48-49 23.146937362240617 26.51773718573336 27.847641050067573 22.487684401958447 50-51 22.68356154228883 26.271912163183796 27.989114308589595 23.055411985937777 52-53 22.19822682150169 26.988856434590286 27.58831322137376 23.224603522534256 54-55 22.05229612102316 26.774368371538635 27.771287180290727 23.40204832714747 56-57 23.1490748925578 26.30819490601336 27.16352935192678 23.37920084950206 58-59 22.164261183535217 26.85863295133769 28.049782157194862 22.927323707932228 60-61 22.33225302633003 27.618983607035368 27.24803432196334 22.800729044671268 62-63 22.692765177332916 26.76468780445802 26.908630526343025 23.63391649186604 64-65 21.859363707466667 26.890937540832365 27.391942295028464 23.857756456672504 66-67 22.43933959865138 26.41652625659739 27.24689822948122 23.89723591527001 68-69 21.942480073953917 26.56848130505881 27.595417474898714 23.89362114608856 70-71 22.049178672561254 26.144879894275334 27.7691335869201 24.036807846243313 72-73 22.76665121765377 26.33964506520419 27.793552353327367 23.100151363814668 74-75 22.98783000737793 26.665323731276846 27.236447731921075 23.110398529424145 76-77 22.636364470121762 26.416674206785228 27.029367586871405 23.9175937362216 78-79 22.48670509470353 26.517501365422707 27.10934195523056 23.886451584643204 80-81 22.124753613475104 26.979528101444927 27.622340432085203 23.27337785299477 82-83 23.17686693645449 25.95221556524751 28.743223906302905 22.127693591995097 84-85 23.311235816182126 26.47880514906414 27.278420640494478 22.931538394259256 86-87 21.89485491684016 27.321036271132154 27.759817186595328 23.02429162543236 88-89 23.01096941874571 25.963996488964707 27.61553821330737 23.409495878982213 90-91 22.941728201461064 25.912173704976844 27.701047122712225 23.445050970849866 92-93 23.34375891460432 26.196199592960195 27.52325394213727 22.936787550298217 94-95 22.04477938533801 26.234203053057154 28.201522223406318 23.519495338198517 96-97 22.386706214555854 26.162968377773378 27.722269506749935 23.728055900920832 98-99 21.81273811644871 26.726770981301414 27.440912417774726 24.019578484475154 100 22.683216083126194 26.10938445660487 26.517940589060622 24.689458871208306 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1787.0 1 1254.5 2 1338.5 3 1804.0 4 1310.0 5 1082.0 6 1618.5 7 2844.0 8 4413.0 9 5191.5 10 4699.5 11 4319.5 12 4701.0 13 5397.0 14 6466.5 15 7717.0 16 8840.5 17 9861.0 18 10753.0 19 11564.0 20 12100.5 21 12523.0 22 13414.0 23 14666.0 24 15905.0 25 17464.0 26 20257.5 27 23424.0 28 28292.5 29 40324.5 30 58785.0 31 76192.0 32 101298.0 33 129484.5 34 150590.0 35 172552.5 36 193099.0 37 225855.0 38 237100.5 39 219590.5 40 200719.5 41 178720.0 42 170466.5 43 179835.0 44 200851.0 45 242096.5 46 279577.5 47 305996.0 48 332106.5 49 336769.5 50 325758.5 51 336289.0 52 340011.5 53 322308.0 54 303614.0 55 261331.5 56 228313.0 57 189896.5 58 150195.5 59 142726.0 60 118231.5 61 81642.0 62 61142.0 63 51420.5 64 38357.0 65 20170.0 66 11775.5 67 7959.5 68 5878.5 69 4552.0 70 3753.5 71 3091.0 72 2525.5 73 2344.5 74 1960.5 75 1521.5 76 1130.5 77 774.5 78 519.0 79 375.0 80 279.5 81 208.0 82 163.5 83 120.0 84 86.5 85 59.5 86 45.0 87 38.0 88 33.0 89 25.5 90 19.0 91 18.0 92 17.5 93 14.5 94 12.0 95 9.5 96 5.0 97 3.5 98 4.0 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05663173646343428 2 0.13109603759865562 3 0.017148275927666302 4 0.016792445780723503 5 0.020035974427855915 6 0.05166380018111755 7 0.056713851112728766 8 0.04924141802693005 9 0.04045515055241946 10-11 0.03949714631065039 12-13 0.040797294924479845 14-15 0.018338938342436426 16-17 0.017531477624373928 18-19 0.017298819451372866 20-21 0.020802377821271173 22-23 0.025277626207820952 24-25 0.024764409649730385 26-27 0.019317471246529116 28-29 0.01800363685781725 30-31 0.01606025682451429 32-33 0.022656800317838437 34-35 0.022779972291780172 36-37 0.022957887365251572 38-39 0.020733948946859093 40-41 0.024162235554904113 42-43 0.031244624056554 44-45 0.04093415267330399 46-47 0.04661374924950632 48-49 0.03979823335806353 50-51 0.02789845209780347 52-53 0.03983929068271077 54-55 0.03697212084484478 56-57 0.03850492763167529 58-59 0.043739736524199116 60-61 0.022998944689898815 62-63 0.025565027480351676 64-65 0.025133925571555592 66-67 0.03229158583505877 68-69 0.03899077264000103 70-71 0.04077676626215622 72-73 0.044642997666438526 74-75 0.02687201898162233 76-77 0.020391804574798715 78-79 0.019529600757206552 80-81 0.024223821541874982 82-83 0.02034390436271026 84-85 0.01242668359323304 86-87 0.01432900630188876 88-89 0.018058379957346916 90-91 0.020597091198034944 92-93 0.019556972306971385 94-95 0.023094745114075724 96-97 0.02449069415208208 98-99 0.027392078427154107 100 0.028849613452131332 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7306857.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.809345723909885 #Duplication Level Percentage of deduplicated Percentage of total 1 78.53845833308739 50.11487640398864 2 13.27875647489711 16.946175253806334 3 4.081185573131283 7.812533435981021 4 1.6234228820927101 4.143582077582398 5 0.838616889526497 2.6755797516853095 6 0.46053825488798067 1.7631986835139977 7 0.2726341992645063 1.2177626913921153 8 0.17750885855076864 0.9061379299458092 9 0.12550552318362268 0.7207582787175569 >10 0.5427572913828537 6.014360801218496 >50 0.03425195202579895 1.5346385247480328 >100 0.02318493247831233 2.8131203204868886 >500 0.002247119073294297 0.9491328279179099 >1k 7.94699297718298E-4 0.9285911407630487 >5k 5.4806848087502356E-5 0.2557144682828054 >10k+ 8.221027213125354E-5 1.2038374099697773 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30246 0.41393994709353144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23192 0.3174004910729743 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15408 0.21087041938825404 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 9163 0.12540275524757089 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.7371549764830488E-5 0.0 12-13 0.0 0.0 0.0 4.105732464724573E-5 0.0 14-15 0.0 0.0 0.0 4.105732464724573E-5 0.0 16-17 0.0 0.0 0.0 4.790021208845335E-5 0.0 18-19 0.0 0.0 0.0 5.4743099529660976E-5 0.0 20-21 0.0 0.0 0.0 1.231719739417372E-4 0.0 22-23 0.0 0.0 0.0 4.379447962372878E-4 0.0 24-25 0.0 0.0 0.0 9.853757915338976E-4 0.0 26-27 0.0 0.0 0.0 0.0019502229207441722 0.0 28-29 0.0 0.0 0.0 0.0053785095287891905 0.0 30-31 0.0 0.0 0.0 0.02065867718500581 0.0 32-33 0.0 0.0 0.0 0.055461602710987774 0.0 34-35 0.0 0.0 0.0 0.10446351967747555 0.0 36-37 0.0 0.0 0.0 0.18497693331072446 0.0 38-39 0.0 0.0 0.0 0.3091411259314367 0.0 40-41 0.0 0.0 0.0 0.4467584352615632 0.0 42-43 0.0 0.0 0.0 0.5753910333813841 0.0 44-45 0.0 0.0 0.0 0.7212129647535185 0.0 46-47 0.0 0.0 0.0 0.8917856200005009 0.0 48-49 0.0 0.0 0.0 1.0835780692026682 0.0 50-51 0.0 0.0 0.0 1.2872634567776542 0.0 52-53 0.0 0.0 0.0 1.481348273272626 0.0 54-55 0.0 0.0 0.0 1.688653548304011 0.0 56-57 0.0 0.0 0.0 1.9298927021563443 0.0 58-59 0.0 0.0 0.0 2.207549702970785 0.0 60-61 0.0 0.0 0.0 2.4855830625944915 0.0 62-63 0.0 0.0 0.0 2.780354124899392 0.0 64-65 0.0 0.0 0.0 3.0651687859773364 0.0 66-67 0.0 0.0 0.0 3.379688421437562 0.0 68-69 0.0 0.0 0.0 3.7243783476260726 0.0 70-71 0.0 0.0 0.0 4.096378237592442 0.0 72-73 0.0 0.0 0.0 4.46241797259752 0.0 74-75 0.0 0.0 0.0 4.809475537840688 0.0 76-77 0.0 0.0 0.0 5.197515155969249 0.0 78-79 0.0 0.0 0.0 5.618482748464901 0.0 80-81 0.0 0.0 0.0 6.059582115812585 0.0 82-83 0.0 0.0 0.0 6.513333982039063 0.0 84-85 0.0 0.0 0.0 6.941123933313598 0.0 86-87 0.0 0.0 0.0 7.393411421627658 0.0 88 0.0 0.0 0.0 7.756974031379018 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 3765 0.0 41.329304 3 GGTATCA 17895 0.0 38.79317 1 TACCTGG 4085 0.0 38.712227 2 GTACTAG 8055 0.0 38.095795 1 GTATCAA 30060 0.0 37.87406 1 GCATCGT 7790 0.0 37.353424 8 GGGCACC 8410 0.0 36.938194 94 TAGGCAT 9570 0.0 35.51554 5 CTAGGCA 10750 0.0 32.44797 4 CTGTGCG 10835 0.0 32.105545 9 CAACGCA 34360 0.0 31.413101 5 TAGTACT 11270 0.0 31.409653 4 ATCAACG 34795 0.0 31.047617 3 CCTGGGG 4150 0.0 30.698227 4 GTACATG 40355 0.0 30.661135 1 TACCCCG 400 0.0 30.55663 5 ACTGTGC 11615 0.0 30.516123 8 CTGGGGG 3965 0.0 30.35211 5 TATCAAC 37895 0.0 29.845081 2 TACATGG 40990 0.0 29.738754 2 >>END_MODULE