FastQCFastQC Report
Mon 6 Jun 2016
ERR1211270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7306857
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283150.3875127157955876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228990.3133905590324267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153640.2102682452934278No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA190850.038.8298231
GGGCACC80400.038.76845694
ACCTGGG36800.037.5508233
TACCTGG38950.035.957552
GTACTAG80350.035.5810741
GTATCAA310500.035.3460271
TAGGCAT97400.033.044335
CTGTGCG109000.032.028069
CCTGGGG37150.032.00974
TAGTACT112200.030.9579014
ACTGTGC116400.030.597198
CTGGGGG32800.030.0822835
GTACATG405950.030.0120891
TACATGG408200.029.5206782
CAACGCA356350.029.4822355
CTAGGCA111500.029.1288384
ATCAACG360750.029.028783
TACTGTG129750.028.7889047
CATGGGT35650.028.7419954
ACATGGG403050.028.722713
Produced by FastQC (version 0.11.5)