FastQCFastQC Report
Mon 6 Jun 2016
ERR1211269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6915578
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302640.4376206876706474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236540.34203937834263454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149830.21665578784593278No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC98810.14288032034343334No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152400.048.049141
GTGGTAT35000.043.5650251
GTATCAA274750.041.3485761
GTACTAG73450.040.1091581
GGGCACC75450.039.1191494
CAACGCA315250.034.5206575
ATCAACG318050.034.246563
GCATCGT79600.034.0137758
TAGGCAT91900.033.8118635
TACCTGG27950.033.675122
TATCAAC347550.032.5655752
TATTCTC35550.032.2651255
ACCTGGG28900.032.0445863
CTGTGCG106150.031.5729379
AACGCAG362200.030.902986
CTAGGCA103950.030.6611424
ACTGTGC113350.030.6041458
TCTAACG41100.030.228952
TAGTACT113650.029.86424
TAACGCC41500.029.3382914
Produced by FastQC (version 0.11.5)