FastQCFastQC Report
Mon 6 Jun 2016
ERR1211269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6915578
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272560.39412468487811136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230580.3334211543850709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146340.21160921039427216No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC101490.1467556291028747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158800.047.3734471
GTGGTAT36450.045.92171
GTATCAA276400.039.8565561
GTACTAG76150.038.5900761
GGGCACC76150.037.47271794
TACCTGG30950.034.925122
TAGGCAT94650.034.3024025
ACCTGGG31150.034.1005943
CAACGCA322750.032.609855
ATCAACG325300.032.3505443
TATTCTC35400.031.7220845
CTAGGCA105500.031.5867794
TATCAAC355900.031.0188542
CTGTGCG103450.030.9750189
ACTGTGC109600.030.1800388
TAGTACT108050.029.7973294
AACGCAG369000.029.2483716
CCTGGGG31250.029.178524
TACCCCG4400.028.8322245
CTGGGGG27450.028.5851975
Produced by FastQC (version 0.11.5)