##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211269_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6915578 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.113295374587636 33.0 31.0 34.0 30.0 34.0 2 32.25651463984644 33.0 31.0 34.0 30.0 34.0 3 32.327684540612516 34.0 31.0 34.0 30.0 34.0 4 35.95514518092342 37.0 35.0 37.0 35.0 37.0 5 35.84658592528347 37.0 35.0 37.0 35.0 37.0 6 35.875591454539304 37.0 35.0 37.0 35.0 37.0 7 35.812785569044266 37.0 35.0 37.0 35.0 37.0 8 35.80765801499166 37.0 35.0 37.0 35.0 37.0 9 37.386443909677546 39.0 37.0 39.0 34.0 39.0 10-11 37.364162258020954 39.0 37.0 39.0 34.0 39.0 12-13 37.240931126798074 39.0 37.0 39.0 33.5 39.0 14-15 38.40583231076275 40.0 38.0 41.0 33.5 41.0 16-17 38.37007485129949 40.0 38.0 41.0 34.0 41.0 18-19 38.41145403898271 40.0 38.0 41.0 34.0 41.0 20-21 38.37755376340199 40.0 38.0 41.0 34.0 41.0 22-23 38.240125915722444 40.0 38.0 41.0 34.0 41.0 24-25 38.1619298488138 40.0 37.0 41.0 33.5 41.0 26-27 38.000050971878274 40.0 37.0 41.0 33.0 41.0 28-29 37.97853411529738 40.0 37.0 41.0 33.0 41.0 30-31 37.97286994666245 40.0 37.0 41.0 33.0 41.0 32-33 37.82232157601288 40.0 36.5 41.0 33.0 41.0 34-35 37.673531915915056 40.0 36.0 41.0 33.0 41.0 36-37 37.53830402896186 40.0 36.0 41.0 32.5 41.0 38-39 37.42742060027376 40.0 36.0 41.0 32.0 41.0 40-41 37.30447852659604 39.0 36.0 41.0 32.0 41.0 42-43 37.13718180895364 39.0 35.0 41.0 31.0 41.0 44-45 36.93476973291314 39.0 35.0 41.0 31.0 41.0 46-47 36.63484534481427 39.0 35.0 41.0 30.5 41.0 48-49 36.32951359958633 39.0 35.0 40.5 30.0 41.0 50-51 36.08617573252735 38.5 35.0 40.0 29.0 41.0 52-53 35.851703791064175 38.0 35.0 40.0 29.0 41.0 54-55 35.58396666193339 38.0 34.5 40.0 28.0 41.0 56-57 35.285507444207845 37.5 34.0 40.0 28.0 41.0 58-59 34.96809521922824 37.0 34.0 40.0 27.0 41.0 60-61 34.68844621519705 36.0 34.0 39.5 27.0 41.0 62-63 34.35550998051067 36.0 33.5 39.0 26.0 41.0 64-65 33.97605832802407 35.0 33.0 39.0 26.0 40.0 66-67 33.680074969872365 35.0 33.0 38.0 26.0 40.0 68-69 33.3787794020977 35.0 32.5 38.0 25.5 40.0 70-71 33.11881689426394 35.0 32.0 37.0 25.0 40.0 72-73 32.82375016231471 35.0 32.0 36.5 25.0 39.5 74-75 32.51716783470594 35.0 32.0 36.0 24.0 39.0 76-77 31.568481043811524 34.0 30.5 35.0 23.5 38.0 78-79 32.101649637962296 34.5 31.5 35.0 24.0 38.0 80-81 32.07231731028122 35.0 32.0 35.0 24.0 37.0 82-83 31.8540528354969 34.0 31.0 35.0 24.0 37.0 84-85 31.63080287721431 34.0 31.0 35.0 24.0 36.0 86-87 31.42475661470379 34.0 31.0 35.0 23.0 36.0 88-89 31.23628820324201 34.0 31.0 35.0 23.0 36.0 90-91 31.030940074712483 34.0 31.0 35.0 21.5 35.0 92-93 30.836832582901962 34.0 30.5 35.0 20.0 35.0 94-95 30.660296579692975 34.0 30.0 35.0 20.0 35.0 96-97 30.501110753143124 34.0 30.0 35.0 19.5 35.0 98-99 30.33735466507644 34.0 29.0 35.0 19.0 35.0 100 28.495906777423375 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4085646691210947 1101 2 -0.3823757431236281 1101 3 -0.42000701936164475 1101 4 -0.18044949130732846 1101 5 -0.26361824810885537 1101 6 -0.29008617819929583 1101 7 -0.3572507560217062 1101 8 -0.3320322005694436 1101 9 -0.4740214794694495 1101 10-11 -0.47600400165577383 1101 12-13 -0.5324506727512741 1101 14-15 -0.6824935813692719 1101 16-17 -0.5504398222040976 1101 18-19 -0.6885473328788407 1101 20-21 -0.7791274231023948 1101 22-23 -0.818625666469174 1101 24-25 -0.8088896983096774 1101 26-27 -0.8847257256415304 1101 28-29 -0.9555661694662589 1101 30-31 -0.7423178740640921 1101 32-33 -0.7094413224963247 1101 34-35 -0.8157135455823834 1101 36-37 -0.7197209488595107 1101 38-39 -0.7812253398213329 1101 40-41 -0.7942444058081932 1101 42-43 -0.7651233659575709 1101 44-45 -0.8551878395618786 1101 46-47 -0.8014204414577293 1101 48-49 -0.7472823526891688 1101 50-51 -0.7661707721140871 1101 52-53 -0.7406802407133668 1101 54-55 -0.6775094754552669 1101 56-57 -0.8229425953771994 1101 58-59 -0.7497273661255193 1101 60-61 -0.7598133555179913 1101 62-63 -0.8174120240107996 1101 64-65 -0.7601425891496874 1101 66-67 -0.9577180301943642 1101 68-69 -1.0353526612323094 1101 70-71 -0.9193855467948353 1101 72-73 -1.0496836971045198 1101 74-75 -1.1538528785940159 1101 76-77 -1.060490588789726 1101 78-79 -0.8910814675268597 1101 80-81 -0.8009832470807261 1101 82-83 -0.8729856567909771 1101 84-85 -0.793704375470643 1101 86-87 -0.8714102672664659 1101 88-89 -0.9755370387540196 1101 90-91 -0.9844063588321283 1101 92-93 -0.9793168371789953 1101 94-95 -1.055741623090686 1101 96-97 -1.1263785563974835 1101 98-99 -1.1526527595502998 1101 100 -1.1184860481969956 1102 1 -0.4096353600692808 1102 2 -0.34568866316750757 1102 3 -0.36309512518430154 1102 4 -0.21341907876966815 1102 5 -0.3305030585140827 1102 6 -0.3056310359943737 1102 7 -0.3168467496665244 1102 8 -0.37761592205290384 1102 9 -0.4872227051674187 1102 10-11 -0.4182203344916715 1102 12-13 -0.4389468411263664 1102 14-15 -0.5267413836673853 1102 16-17 -0.6683599362052774 1102 18-19 -0.6463847147113881 1102 20-21 -0.6530972304410696 1102 22-23 -0.714311535883219 1102 24-25 -0.7313915513049167 1102 26-27 -0.7778635384394192 1102 28-29 -0.7790246324599437 1102 30-31 -0.603172257650634 1102 32-33 -0.5259715067305564 1102 34-35 -0.6192494760638994 1102 36-37 -0.7628011679536044 1102 38-39 -0.7443835744858589 1102 40-41 -0.6963203008393108 1102 42-43 -0.6310664778136399 1102 44-45 -0.6318612637697072 1102 46-47 -0.7260949788136131 1102 48-49 -0.7597978318413823 1102 50-51 -0.83347853246201 1102 52-53 -0.8385395921669669 1102 54-55 -0.8804392600266411 1102 56-57 -0.9175615821165621 1102 58-59 -0.9263279957000279 1102 60-61 -0.9292061970702719 1102 62-63 -0.9250010124909025 1102 64-65 -0.874491404615803 1102 66-67 -0.9146010535415243 1102 68-69 -0.9559367654729911 1102 70-71 -0.9954772829571326 1102 72-73 -0.9855315416538843 1102 74-75 -1.0710620291032882 1102 76-77 -0.8684735277244471 1102 78-79 -0.6593697939720649 1102 80-81 -0.6241191261505428 1102 82-83 -0.6605177167285738 1102 84-85 -0.7998793880244328 1102 86-87 -0.7175605980909303 1102 88-89 -0.7478990985204987 1102 90-91 -0.7597262119923052 1102 92-93 -0.9628664594330019 1102 94-95 -0.9957348891368021 1102 96-97 -1.0295462294047084 1102 98-99 -1.1366604939479252 1102 100 -1.1158353354515924 1103 1 -0.3743106683086097 1103 2 -0.3886900947752707 1103 3 -0.37604104091500545 1103 4 -0.20028434547295149 1103 5 -0.24737791958312272 1103 6 -0.3220872326735531 1103 7 -0.41333481675525263 1103 8 -0.40101626780438693 1103 9 -0.6574848854085715 1103 10-11 -0.5373446827204376 1103 12-13 -0.5656918286474308 1103 14-15 -0.7415634276931371 1103 16-17 -0.7064184991264426 1103 18-19 -0.7617166756174498 1103 20-21 -0.7799318795793155 1103 22-23 -0.7979147640476114 1103 24-25 -0.8863230331570406 1103 26-27 -0.8239528295994916 1103 28-29 -0.9181293307312259 1103 30-31 -0.8999439761697161 1103 32-33 -0.7520861419017066 1103 34-35 -0.8944948466290583 1103 36-37 -0.9468911549743666 1103 38-39 -0.9054646851210677 1103 40-41 -0.8795644396358497 1103 42-43 -0.772913917954746 1103 44-45 -0.8830023080733866 1103 46-47 -0.9813542340115546 1103 48-49 -1.032177581485783 1103 50-51 -1.167567696046774 1103 52-53 -1.1452416434185935 1103 54-55 -1.0023094427828525 1103 56-57 -1.0855405792295514 1103 58-59 -1.0874144007614603 1103 60-61 -1.0924216741849975 1103 62-63 -0.9201450691194779 1103 64-65 -1.0271535884816032 1103 66-67 -1.0682107997045875 1103 68-69 -1.1499767229658744 1103 70-71 -1.1641082326608725 1103 72-73 -1.0853418102902737 1103 74-75 -1.0613893542046569 1103 76-77 -1.094306171064229 1103 78-79 -0.923018843868185 1103 80-81 -0.8004911378892032 1103 82-83 -0.8585688384354917 1103 84-85 -0.9827221431328645 1103 86-87 -0.9163300818021547 1103 88-89 -1.0899250397326696 1103 90-91 -1.0369473835169778 1103 92-93 -1.0976250955776052 1103 94-95 -1.165966448249364 1103 96-97 -1.2439258197581538 1103 98-99 -1.1106272020358432 1103 100 -1.2482249576736635 1104 1 -0.3990755052187396 1104 2 -0.3414030417234528 1104 3 -0.4042391301237842 1104 4 -0.31185742023296825 1104 5 -0.30776604859082823 1104 6 -0.4206186097953051 1104 7 -0.4015960222784827 1104 8 -0.3873209273633407 1104 9 -0.582281840820805 1104 10-11 -0.5685823768870932 1104 12-13 -0.6287833176902211 1104 14-15 -0.8399657633686459 1104 16-17 -0.7603320482984373 1104 18-19 -0.7333652135315134 1104 20-21 -0.7886510814151606 1104 22-23 -0.7206949085562968 1104 24-25 -0.7559028396044951 1104 26-27 -0.8680417370787268 1104 28-29 -0.9173844038979055 1104 30-31 -0.8687149328970278 1104 32-33 -0.8980798445068672 1104 34-35 -0.7748940715798938 1104 36-37 -0.783146451240377 1104 38-39 -0.7428868641234416 1104 40-41 -0.8023959702014096 1104 42-43 -0.7601851850882966 1104 44-45 -0.8191705406278587 1104 46-47 -0.8358369385439488 1104 48-49 -0.854319596537124 1104 50-51 -0.8379558937043257 1104 52-53 -0.8611651957598809 1104 54-55 -0.884269995770282 1104 56-57 -0.8534963202933668 1104 58-59 -0.8582178438628603 1104 60-61 -0.8397178518400565 1104 62-63 -0.7814978753998147 1104 64-65 -0.9719729683282736 1104 66-67 -1.0051351811890967 1104 68-69 -0.9639795007261327 1104 70-71 -1.127525881938631 1104 72-73 -1.12305834007649 1104 74-75 -1.0382525446102768 1104 76-77 -1.0016198850819862 1104 78-79 -0.7715898593101223 1104 80-81 -0.823259619523558 1104 82-83 -0.7730938135410561 1104 84-85 -0.8276587191607057 1104 86-87 -0.8882919264706537 1104 88-89 -0.9627064369110698 1104 90-91 -0.9130410344726343 1104 92-93 -0.9794753654994324 1104 94-95 -0.9712465916814388 1104 96-97 -1.058804313709203 1104 98-99 -1.1179277205904405 1104 100 -1.2722951645293321 1105 1 -0.41183601037995743 1105 2 -0.3813257650088211 1105 3 -0.44241924695488066 1105 4 -0.2609293681112561 1105 5 -0.2835608034688093 1105 6 -0.3439790909109206 1105 7 -0.35275090458543445 1105 8 -0.40175861967249915 1105 9 -0.6082581654440702 1105 10-11 -0.5530258261461043 1105 12-13 -0.5593331668326869 1105 14-15 -0.8478432173082737 1105 16-17 -0.748619676963088 1105 18-19 -0.6833667720427883 1105 20-21 -0.7711471549209179 1105 22-23 -0.790306864691523 1105 24-25 -0.8139763924313499 1105 26-27 -0.8443177984090084 1105 28-29 -0.8214893572284367 1105 30-31 -0.8003883987017062 1105 32-33 -0.7942775349839835 1105 34-35 -0.8162252437775805 1105 36-37 -0.7017320863464533 1105 38-39 -0.8026109122070082 1105 40-41 -0.9146777006960036 1105 42-43 -0.8287937525746969 1105 44-45 -0.7407481484466274 1105 46-47 -0.7064518634841406 1105 48-49 -0.7996840051011773 1105 50-51 -0.8327707075767279 1105 52-53 -0.7849596427523693 1105 54-55 -0.6895051814843995 1105 56-57 -0.8441054819224405 1105 58-59 -0.9048613130962551 1105 60-61 -0.718138195692184 1105 62-63 -0.847843369998138 1105 64-65 -0.8691109818179825 1105 66-67 -0.8208992747430344 1105 68-69 -0.7308882094963494 1105 70-71 -0.8626372073538775 1105 72-73 -0.8042187884951737 1105 74-75 -0.9130094200305194 1105 76-77 -0.9071594067017195 1105 78-79 -0.7578029377006956 1105 80-81 -0.812836843121584 1105 82-83 -0.7440220565008815 1105 84-85 -0.7303932894218939 1105 86-87 -0.6853385157800318 1105 88-89 -0.79049251756053 1105 90-91 -0.744346933342424 1105 92-93 -0.7976760546794814 1105 94-95 -0.8624252015275395 1105 96-97 -0.9233694540738142 1105 98-99 -0.9587238081546019 1105 100 -1.0286753471359624 1106 1 -0.4531334809047429 1106 2 -0.4192253715903185 1106 3 -0.522231952314975 1106 4 -0.342310759603194 1106 5 -0.38627719923160697 1106 6 -0.4015008660950059 1106 7 -0.48525674974937516 1106 8 -0.4677037904759871 1106 9 -0.5759070690471901 1106 10-11 -0.6314161771053293 1106 12-13 -0.7467663449184698 1106 14-15 -0.835234933697798 1106 16-17 -0.7909021901030187 1106 18-19 -0.7836520160433196 1106 20-21 -0.7915812245890308 1106 22-23 -0.8820017440161578 1106 24-25 -0.8033828355165937 1106 26-27 -0.8441280721808937 1106 28-29 -0.838189567342603 1106 30-31 -0.9512120212693347 1106 32-33 -0.8855321721473786 1106 34-35 -0.849742068497477 1106 36-37 -0.8595139167712276 1106 38-39 -0.9033600907666397 1106 40-41 -0.8996001704414383 1106 42-43 -0.8254809942184451 1106 44-45 -0.9834472113499402 1106 46-47 -0.9983734416472245 1106 48-49 -1.0787070677800443 1106 50-51 -1.080174145468355 1106 52-53 -0.9518392916060918 1106 54-55 -0.9710925097924488 1106 56-57 -0.9605543032256421 1106 58-59 -0.9594678010723641 1106 60-61 -0.9646282916463917 1106 62-63 -1.0641385024573466 1106 64-65 -0.9152203783476764 1106 66-67 -0.8928868798780556 1106 68-69 -0.9032234070473066 1106 70-71 -1.007189327511746 1106 72-73 -1.0697040150381412 1106 74-75 -1.1866370378846085 1106 76-77 -1.0867474707210718 1106 78-79 -0.8671474688094385 1106 80-81 -0.8596749679306406 1106 82-83 -0.9021690615947442 1106 84-85 -0.8656314291945719 1106 86-87 -0.924890168324783 1106 88-89 -0.9813217514855452 1106 90-91 -1.0122123106438643 1106 92-93 -1.0376076584677705 1106 94-95 -1.0317514645505632 1106 96-97 -1.0363287592630748 1106 98-99 -1.061406099334171 1106 100 -1.0110442128312407 1107 1 -0.44308435848082084 1107 2 -0.44975956957025787 1107 3 -0.4943095559758355 1107 4 -0.2841573375066275 1107 5 -0.32322657124226595 1107 6 -0.36549769214856553 1107 7 -0.4597820943916915 1107 8 -0.49098843236786394 1107 9 -0.5934175036115974 1107 10-11 -0.5726491078052689 1107 12-13 -0.5780538044779462 1107 14-15 -0.7651387719318805 1107 16-17 -0.749237462857451 1107 18-19 -0.7723278798758457 1107 20-21 -0.7237344800390488 1107 22-23 -0.7499049472694068 1107 24-25 -0.7735723049867573 1107 26-27 -0.8415552744534196 1107 28-29 -0.7702772655705914 1107 30-31 -0.7868927484995751 1107 32-33 -0.8210750328749086 1107 34-35 -0.8350467874681726 1107 36-37 -0.8161697945101238 1107 38-39 -0.7420233603337252 1107 40-41 -0.7915015070617315 1107 42-43 -0.6944036386119379 1107 44-45 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1.0082797126609577 2311 34-35 1.0456337377418237 2311 36-37 1.0664485245433255 2311 38-39 1.0576435217704798 2311 40-41 1.021201461781807 2311 42-43 1.0310084094935448 2311 44-45 1.0796882368533005 2311 46-47 0.8166194649094507 2311 48-49 1.0742177709720977 2311 50-51 1.1297416107512106 2311 52-53 1.0321318665996344 2311 54-55 1.1257186583587213 2311 56-57 1.2625189859382075 2311 58-59 1.339966478540937 2311 60-61 1.3214820150536255 2311 62-63 1.295228206623726 2311 64-65 1.319467290761473 2311 66-67 1.3357943246910082 2311 68-69 1.321861787400799 2311 70-71 1.3715693930358412 2311 72-73 1.3714322522018492 2311 74-75 1.3778416305956398 2311 76-77 1.3465629003318327 2311 78-79 1.047899113905963 2311 80-81 1.0391801356921313 2311 82-83 1.142299076412943 2311 84-85 1.2535882920404156 2311 86-87 1.2595225384454345 2311 88-89 1.3089219119488504 2311 90-91 1.3474238030267394 2311 92-93 1.366040418989705 2311 94-95 1.36984521835058 2311 96-97 1.4374812242099324 2311 98-99 1.4451773473479967 2311 100 1.4889788957057526 2312 1 0.26971319148575645 2312 2 0.39806119086028247 2312 3 0.4836329193953617 2312 4 0.17691785109802538 2312 5 0.3419871337097504 2312 6 0.41381837861764836 2312 7 0.35096347559568386 2312 8 0.41464018366216493 2312 9 0.6353040034545003 2312 10-11 0.6589654626728318 2312 12-13 0.7457032303249562 2312 14-15 0.9367173163737448 2312 16-17 0.913517355081467 2312 18-19 0.8865874329173096 2312 20-21 0.8976144241227857 2312 22-23 0.9399662847934067 2312 24-25 0.9347204661476027 2312 26-27 0.999783568061531 2312 28-29 1.0070396709821594 2312 30-31 0.985196461756324 2312 32-33 0.9085238567703229 2312 34-35 1.020674208108204 2312 36-37 1.1048713559364884 2312 38-39 0.9354910995731345 2312 40-41 1.0098549959052505 2312 42-43 1.0920973132783018 2312 44-45 0.8428697843783368 2312 46-47 0.8692936910358 2312 48-49 1.107502579640098 2312 50-51 0.9408851808236065 2312 52-53 1.0695694881891313 2312 54-55 0.9494653053254041 2312 56-57 1.016167004299902 2312 58-59 1.1278245263473181 2312 60-61 1.1304776550867004 2312 62-63 1.156627139502227 2312 64-65 1.1834821803936038 2312 66-67 1.2334076083766305 2312 68-69 1.3351108459778303 2312 70-71 1.2176625374645695 2312 72-73 1.1777468225865988 2312 74-75 1.2501382346879453 2312 76-77 1.3037936018922665 2312 78-79 1.1992275409604503 2312 80-81 1.1509386868775842 2312 82-83 1.1229823055726662 2312 84-85 1.194807866151077 2312 86-87 1.2217485197304505 2312 88-89 1.2744810042989627 2312 90-91 1.3163448545192686 2312 92-93 1.3393870884171761 2312 94-95 1.381325583291627 2312 96-97 0.9535414378448017 2312 98-99 0.7330429288777403 2312 100 0.9506465391828236 2313 1 0.06705557594374767 2313 2 0.21419883378842286 2313 3 0.3164365072784605 2313 4 0.2095276974690492 2313 5 0.3435151414561517 2313 6 0.2648739125469888 2313 7 0.2677651370439307 2313 8 0.42134564719783896 2313 9 0.6369923111409719 2313 10-11 0.39375180606513993 2313 12-13 0.4408252931939387 2313 14-15 0.5233554822902491 2313 16-17 0.7436853528273772 2313 18-19 0.726660980544878 2313 20-21 0.7120559766424819 2313 22-23 0.6868502737147324 2313 24-25 0.5609836973216957 2313 26-27 0.7929080565756834 2313 28-29 0.6494863711389698 2313 30-31 0.8414807556004789 2313 32-33 0.9098480808800744 2313 34-35 0.9728775185674294 2313 36-37 0.9903783152811627 2313 38-39 1.0025998821622082 2313 40-41 1.014528150918622 2313 42-43 1.0464762866802602 2313 44-45 0.9225718406654835 2313 46-47 0.9213504781794484 2313 48-49 0.9778976648451376 2313 50-51 1.000030805973843 2313 52-53 0.9802466678048987 2313 54-55 0.8904397165952886 2313 56-57 0.6932604369781714 2313 58-59 0.7642236292166729 2313 60-61 0.6395118643880195 2313 62-63 0.9524921189893973 2313 64-65 1.0466683685477491 2313 66-67 1.0035357145443058 2313 68-69 1.1200570059066521 2313 70-71 1.073414167337738 2313 72-73 1.1144424335884153 2313 74-75 1.198599639573942 2313 76-77 1.0996134536582645 2313 78-79 0.944423433354423 2313 80-81 0.8777520388490352 2313 82-83 0.971311745056358 2313 84-85 0.9629526376302344 2313 86-87 0.9942087728339182 2313 88-89 1.041087315655112 2313 90-91 0.8586881560865791 2313 92-93 1.0592623850787746 2313 94-95 1.168360574515173 2313 96-97 1.2700929752785406 2313 98-99 1.2360031233770137 2313 100 1.4689831524960653 2314 1 -0.3046557373890799 2314 2 0.06446975115139253 2314 3 0.28661915564772755 2314 4 0.21080829661445222 2314 5 0.35212414902889577 2314 6 0.3878891756677376 2314 7 0.4500539705884279 2314 8 0.4489257434666598 2314 9 0.4917404200954252 2314 10-11 0.5871736127845679 2314 12-13 0.42617167769452635 2314 14-15 0.764717191942303 2314 16-17 0.7851694269761822 2314 18-19 0.7814756334830264 2314 20-21 0.8421558460927869 2314 22-23 0.8973331079622469 2314 24-25 0.8270109009984807 2314 26-27 0.731457208073941 2314 28-29 0.7891242828856591 2314 30-31 0.7894218278477183 2314 32-33 0.8316115713127274 2314 34-35 0.9020019657275995 2314 36-37 0.8822078933796149 2314 38-39 0.6703479034180617 2314 40-41 0.6219688491990851 2314 42-43 0.8168214198716157 2314 44-45 0.9544026094684597 2314 46-47 0.8822781867292093 2314 48-49 0.8468533955919071 2314 50-51 0.8114229320658808 2314 52-53 0.8872785052473517 2314 54-55 0.779022129390448 2314 56-57 0.8201473293178054 2314 58-59 0.8840045253252029 2314 60-61 0.9596637326889734 2314 62-63 0.910652822745682 2314 64-65 0.9102485033183925 2314 66-67 0.6495851779786221 2314 68-69 0.6031738853196487 2314 70-71 0.7829954101052792 2314 72-73 0.9541475096259546 2314 74-75 1.016097819649815 2314 76-77 0.8188119740474775 2314 78-79 0.7051092007278399 2314 80-81 0.7690252941282374 2314 82-83 0.8225134582448739 2314 84-85 0.8779411833379491 2314 86-87 0.8959774039729105 2314 88-89 0.8860293240670458 2314 90-91 0.9884367249239183 2314 92-93 0.9176963020474567 2314 94-95 0.8890116545982529 2314 96-97 0.9223015895622879 2314 98-99 1.0163864018927846 2314 100 1.0887333612258558 2315 1 0.41401554663599427 2315 2 0.45117998150949035 2315 3 0.45322583220205104 2315 4 0.3460068519126054 2315 5 0.4048521182377698 2315 6 0.42105479299854665 2315 7 0.3779200185515208 2315 8 0.2755215033733762 2315 9 0.4931853183867858 2315 10-11 0.5741116755426603 2315 12-13 0.4034437926757306 2315 14-15 0.6305676731587866 2315 16-17 0.7350628563610471 2315 18-19 0.7665262808346256 2315 20-21 0.469178618107442 2315 22-23 0.8548829239367493 2315 24-25 0.8820905798975005 2315 26-27 0.8232209061514624 2315 28-29 0.7690249526177766 2315 30-31 0.8016775608416751 2315 32-33 0.7830220218278967 2315 34-35 0.8593730601846659 2315 36-37 0.8644202263850715 2315 38-39 0.9119655176675181 2315 40-41 0.9136509670848554 2315 42-43 1.004030785176603 2315 44-45 0.9900807956772866 2315 46-47 0.957802024450011 2315 48-49 0.9194090417297573 2315 50-51 0.9654432471332441 2315 52-53 1.0440118036885693 2315 54-55 0.9209374397782355 2315 56-57 0.8349929878456308 2315 58-59 0.8457220872364886 2315 60-61 0.9565106596807311 2315 62-63 1.0313347904924584 2315 64-65 1.0611065686583174 2315 66-67 1.095067959001618 2315 68-69 1.1305880009098317 2315 70-71 1.0166330391441178 2315 72-73 1.0498843342285369 2315 74-75 0.46440255407920716 2315 76-77 0.41595539045303553 2315 78-79 0.7550985226188018 2315 80-81 0.8381233096276297 2315 82-83 0.7522774313433338 2315 84-85 0.8387350537385423 2315 86-87 0.8486496954726555 2315 88-89 0.8998096552988954 2315 90-91 0.928799107110418 2315 92-93 0.9892677801306746 2315 94-95 1.0366037620584798 2315 96-97 1.176063272690456 2315 98-99 1.1737426929266768 2315 100 1.0842859638921567 2316 1 0.2920633624726392 2316 2 0.06751157838155564 2316 3 0.21810799165887573 2316 4 3.493131305560837E-4 2316 5 0.2575078519214955 2316 6 0.37384985428102624 2316 7 0.4160715044734644 2316 8 0.43331314546150423 2316 9 0.6541939005505526 2316 10-11 0.6710905198577777 2316 12-13 0.40532544663751224 2316 14-15 0.5516660292168964 2316 16-17 0.8034673486001367 2316 18-19 0.8105855326175657 2316 20-21 0.7445149889118241 2316 22-23 0.801985413408417 2316 24-25 0.4705669525753393 2316 26-27 0.7449262219508341 2316 28-29 0.9610967396651162 2316 30-31 0.5951847062686895 2316 32-33 0.702903940773723 2316 34-35 0.848438473831223 2316 36-37 0.9130321544365572 2316 38-39 0.8253157205265822 2316 40-41 0.8208933720710476 2316 42-43 0.7598113052485829 2316 44-45 0.6043595819603382 2316 46-47 0.7088155602964221 2316 48-49 0.7957612128408158 2316 50-51 0.8692828575223075 2316 52-53 0.9301593829242876 2316 54-55 0.7897596335142083 2316 56-57 0.883345473449431 2316 58-59 0.8082817965529259 2316 60-61 0.9085526683723231 2316 62-63 0.8850347497884314 2316 64-65 0.9774544527663309 2316 66-67 0.9857212656340053 2316 68-69 1.0138812772068988 2316 70-71 0.9220523145112125 2316 72-73 0.9416540900972166 2316 74-75 0.9853448733534833 2316 76-77 0.8278285182296372 2316 78-79 0.5503064042907724 2316 80-81 0.5301570649944658 2316 82-83 0.5378203012330722 2316 84-85 0.6422999471218525 2316 86-87 0.7362855455074389 2316 88-89 0.7210713337956136 2316 90-91 0.6585755640936881 2316 92-93 0.4738401107891299 2316 94-95 0.46132657665037513 2316 96-97 0.6995889613747082 2316 98-99 0.8732284776015433 2316 100 0.5493933035282836 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 6.0 12 36.0 13 165.0 14 678.0 15 1935.0 16 4726.0 17 9856.0 18 16991.0 19 25656.0 20 37862.0 21 53734.0 22 65299.0 23 64345.0 24 61477.0 25 64039.0 26 73789.0 27 90261.0 28 111301.0 29 136039.0 30 168967.0 31 210891.0 32 268581.0 33 358281.0 34 529058.0 35 718691.0 36 1102828.0 37 1507112.0 38 1057359.0 39 175604.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.00184328757326 21.65715342989352 13.628321067654383 22.71268221487884 2 15.439099956648597 20.104668040762462 41.2992522100105 23.156979792578436 3 18.765115333109588 27.239274466902764 29.246019529534557 24.749590670453095 4 12.321750912644875 16.62455127178656 39.57065007567463 31.483047739893934 5 12.651437088844924 39.157464495375514 34.16010924900276 14.030989166776806 6 29.40543798363636 38.216125969514046 17.944183407373902 14.43425263947569 7 27.02998238167787 32.74648009522917 22.82642771455773 17.397109808535227 8 27.28632371726557 34.91363990110443 19.30201640412414 18.498019977505855 9 27.24086686805044 15.062567029905605 21.608200006247447 36.08836609579651 10-11 26.532717676688584 26.175110182960324 26.309828454540735 20.982343685810356 12-13 26.278671139274255 23.85876495066645 27.539375595214167 22.323188314845122 14-15 23.725668480979216 24.881430546475798 25.57034730485584 25.822553667689142 16-17 21.65619583319295 27.56479989542422 26.938545387292624 23.84045888409021 18-19 21.0238609122766 27.52365745856673 29.834433217295793 21.618048411860872 20-21 22.804836096828943 26.676928042302183 29.089835887880362 21.428399972988515 22-23 23.13771892963972 26.241834015898597 28.834032383121123 21.78641467134056 24-25 22.34928232259604 26.148444715974716 29.844180586107733 21.658092375321512 26-27 22.159593564756022 26.570505970464787 28.714112357353915 22.555788107425276 28-29 21.310047886188315 27.361296923866224 29.186419213241322 22.142235976704143 30-31 22.7340941170108 26.54787212274957 29.161598880645307 21.55643487959432 32-33 23.139773771430427 26.801787909577122 28.17635204461082 21.882086274381628 34-35 22.802473986554823 26.774099929843015 29.173988095225177 21.249437988376982 36-37 22.294291345772272 26.45680877834133 28.82928713548546 22.41961274040094 38-39 22.678034052258376 26.483734315179547 29.37024098540226 21.467990647159816 40-41 22.217458362338252 25.42208119911607 30.02593885556115 22.334521582984525 42-43 22.238240018323864 25.74976444464753 29.01065884354902 23.001336693479583 44-45 22.10672874631812 26.84862734095735 28.944914673620858 22.09972923910367 46-47 22.16058102793523 26.50369759183778 28.149830116508586 23.185891263718403 48-49 23.21031512682965 26.45095695328015 28.15248840678325 22.186239513106948 50-51 22.74559540926874 26.24813940817543 28.086131165712974 22.92013401684285 52-53 22.00997437460226 26.985368164707698 27.734476655536707 23.270180805153338 54-55 21.93519039337704 26.792371408537953 27.847317683980187 23.425120514104815 56-57 23.178832749552424 26.17542694718035 27.28080524603173 23.3649350572355 58-59 21.87276550702424 26.9431995094157 28.251583383098193 22.932451600461864 60-61 22.333578683726472 27.583351650258557 27.31656563436332 22.766504031651646 62-63 22.79359612001744 26.759306456009295 26.877555402758585 23.56954202121468 64-65 21.719773319742682 26.86165244159066 27.379525394268594 24.03904884439806 66-67 22.290051871197377 26.4358193406498 27.407293799330084 23.86683498882273 68-69 21.71961957477938 26.590967360705985 27.65766504847626 24.031748016038375 70-71 21.743816100920707 26.117722835827923 27.902298948273646 24.236162114977724 72-73 22.418323469599883 26.412965530291466 27.834260344406303 23.334450655702344 74-75 22.677798853684745 26.78308754659808 27.281351207206832 23.257762392510344 76-77 22.28999697830615 26.45877731186701 27.03177812290758 24.219447586919262 78-79 22.02479623308523 26.487746506697935 27.329200676026876 24.15825658418996 80-81 21.6649526411273 27.052391340372793 27.715619448155117 23.567036570344793 82-83 22.777421800247204 25.891206564201553 28.812662201529477 22.518709434021762 84-85 23.038664776985396 26.485165346146744 27.23409574526572 23.24207413160214 86-87 21.454898433588415 27.3671007629752 27.69005238541247 23.487948418023915 88-89 22.393232407225923 25.93796763988942 27.692775528729037 23.976024424155614 90-91 22.34416701966857 25.770093910080575 27.93901573809805 23.946723332152803 92-93 22.69745393157491 26.192708759022192 27.518731515308886 23.591105794094013 94-95 21.361765600614522 26.222210202663028 28.160238785576517 24.255785411145936 96-97 21.648849760431226 26.14977682954798 27.735851675882405 24.465521734138385 98-99 20.870035335888364 26.779169536115134 27.636652217639217 24.714142910357285 100 21.559731626723693 26.259070124553112 26.854887843183544 25.326310405539655 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1888.0 1 1322.5 2 1270.5 3 1679.5 4 1253.5 5 1050.0 6 1624.0 7 2960.0 8 4493.5 9 5159.5 10 4496.0 11 3763.0 12 3978.0 13 4702.5 14 5736.0 15 6989.5 16 8327.5 17 9546.5 18 10613.0 19 11409.0 20 12133.0 21 12666.5 22 13382.5 23 14444.5 24 15271.0 25 16595.5 26 18887.0 27 21358.0 28 25779.5 29 37026.5 30 54508.5 31 71702.0 32 96681.0 33 125035.5 34 145413.0 35 167199.5 36 187046.5 37 220187.0 38 233336.5 39 211470.5 40 188708.0 41 166381.5 42 157087.5 43 165800.5 44 186231.5 45 228543.5 46 267645.5 47 295019.0 48 318355.5 49 319811.5 50 312924.0 51 323350.0 52 326579.0 53 309682.5 54 294396.0 55 253726.5 56 217202.0 57 179742.0 58 139817.5 59 131896.5 60 106629.5 61 69491.0 62 50343.0 63 42238.5 64 29995.0 65 13454.0 66 7164.0 67 4916.0 68 3610.5 69 2743.5 70 2266.5 71 1823.0 72 1510.0 73 1490.5 74 1285.0 75 893.5 76 535.5 77 298.5 78 196.0 79 137.5 80 83.0 81 49.5 82 29.5 83 21.5 84 14.0 85 6.0 86 4.0 87 3.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042353654314939405 2 0.0 3 1.446010731134838E-5 4 0.03512360065926522 5 0.0 6 0.0 7 7.23005365567419E-4 8 0.0 9 0.010902920912756678 10-11 5.061037558971933E-4 12-13 0.0 14-15 1.5906118042483217E-4 16-17 2.747420389156192E-4 18-19 0.0 20-21 1.446010731134838E-5 22-23 0.0 24-25 5.856343461096093E-4 26-27 5.205638632085417E-4 28-29 2.024415023588773E-4 30-31 0.0023859177063724826 32-33 0.014785459725853716 34-35 0.02599204289214871 36-37 0.031139841094988734 38-39 0.022102274025395996 40-41 0.0012146490141532638 42-43 8.676064386809028E-5 44-45 0.011647616439291119 46-47 0.0185161674121816 48-49 0.003173993554840969 50-51 0.011213813219950668 52-53 0.010967991395657746 54-55 0.010461887639760551 56-57 3.904228974064062E-4 58-59 0.00807596993338807 60-61 0.004309111978781817 62-63 0.0012001889068419154 64-65 0.03604181747353583 66-67 0.06113733371238095 68-69 0.06006728577134117 70-71 0.052873382383945344 72-73 0.04654708543523044 74-75 0.03573092516634185 76-77 0.03234726005548632 78-79 0.015327713750029281 80-81 0.0012146490141532638 82-83 0.038875998506560115 84-85 0.052837232115666974 86-87 0.05807902101603076 88-89 0.07054363351841307 90-91 0.049656008507170335 92-93 0.039953276501255575 94-95 0.01333944899471888 96-97 0.0034125853254782174 98-99 0.02172631123530094 100 0.0321303584458161 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6915578.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.0336551992013 #Duplication Level Percentage of deduplicated Percentage of total 1 77.43046427232852 48.807251868560314 2 13.899959263845377 17.52330479040347 3 4.313940972659281 8.157704034609365 4 1.7695716037228522 4.461702652774557 5 0.8429055478839783 2.656570883540627 6 0.4873579267387334 1.8431970907588155 7 0.30012506103843345 1.3242585729895098 8 0.19826622232025723 0.9997955756306628 9 0.12935474896563348 0.7338332380211083 >10 0.5693724416925853 6.28683527791469 >50 0.03538551302656279 1.5342134307958732 >100 0.020613738197027946 2.383506800641914 >500 0.0016552797692649392 0.7335873371918998 >1k 8.847122821526178E-4 1.1010315317685444 >5k 2.8539105873772303E-5 0.10226883845852813 >10k+ 1.1415642349508921E-4 1.3509380759402088 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27256 0.39412468487811136 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23058 0.3334211543850709 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14634 0.21160921039427216 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 10149 0.1467556291028747 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.446010731134838E-5 2 0.0 0.0 0.0 0.0 1.446010731134838E-5 3 0.0 0.0 0.0 0.0 1.446010731134838E-5 4 0.0 0.0 0.0 0.0 1.446010731134838E-5 5 0.0 0.0 0.0 0.0 1.446010731134838E-5 6 0.0 0.0 0.0 0.0 1.446010731134838E-5 7 0.0 0.0 0.0 0.0 1.446010731134838E-5 8 0.0 0.0 0.0 0.0 1.446010731134838E-5 9 0.0 0.0 0.0 1.446010731134838E-5 1.446010731134838E-5 10-11 0.0 0.0 0.0 2.892021462269676E-5 1.446010731134838E-5 12-13 0.0 0.0 0.0 4.338032193404514E-5 1.446010731134838E-5 14-15 0.0 0.0 0.0 4.338032193404514E-5 1.446010731134838E-5 16-17 0.0 0.0 0.0 5.784042924539352E-5 1.446010731134838E-5 18-19 0.0 0.0 0.0 9.399069752376446E-5 2.169016096702257E-5 20-21 0.0 0.0 0.0 1.3014096580213542E-4 2.892021462269676E-5 22-23 0.0 0.0 0.0 4.048830047177546E-4 2.892021462269676E-5 24-25 0.0 0.0 0.0 0.001033897672761409 2.892021462269676E-5 26-27 0.0 0.0 0.0 0.002320847223471415 2.892021462269676E-5 28-29 0.0 0.0 0.0 0.006000944534209577 2.892021462269676E-5 30-31 0.0 0.0 0.0 0.02067795345522818 2.892021462269676E-5 32-33 0.0 0.0 0.0 0.054543524778406086 2.892021462269676E-5 34-35 0.0 0.0 0.0 0.10127859160868405 2.892021462269676E-5 36-37 0.0 0.0 0.0 0.17907396894373834 2.892021462269676E-5 38-39 0.0 0.0 0.0 0.30160168824644884 2.892021462269676E-5 40-41 0.0 0.0 0.0 0.43627589769069197 2.892021462269676E-5 42-43 0.0 0.0 0.0 0.5641321665376343 2.892021462269676E-5 44-45 0.0 0.0 0.0 0.7045614986917941 2.892021462269676E-5 46-47 0.0 0.0 0.0 0.865936296286442 2.892021462269676E-5 48-49 0.0 0.0 0.0 1.0495941192478777 3.615026827837095E-5 50-51 0.0 0.0 0.0 1.2451381504192418 4.338032193404514E-5 52-53 0.0 0.0 0.0 1.4343414245345798 4.338032193404514E-5 54-55 0.0 0.0 0.0 1.6373251809176326 4.338032193404514E-5 56-57 0.0 0.0 0.0 1.867132436363237 5.0610375589719324E-5 58-59 0.0 0.0 0.0 2.138584800865524 5.784042924539352E-5 60-61 0.0 0.0 0.0 2.410116695958024 5.784042924539352E-5 62-63 0.0 0.0 0.0 2.6965208114202457 5.784042924539352E-5 64-65 0.0 0.0 0.0 2.976584169826441 5.784042924539352E-5 66-67 0.0 0.0 0.0 3.2839699009974295 5.784042924539352E-5 68-69 0.0 0.0 0.0 3.617239224255731 5.784042924539352E-5 70-71 0.0 0.0 0.0 3.9810916744775344 5.784042924539352E-5 72-73 0.0 0.0 0.0 4.341777361198153 5.784042924539352E-5 74-75 0.0 0.0 0.0 4.684792796784304 5.784042924539352E-5 76-77 0.0 0.0 0.0 5.079676637296261 5.784042924539352E-5 78-79 0.0 0.0 0.0 5.496597681350712 5.784042924539352E-5 80-81 0.0 0.0 0.0 5.929909257042578 5.784042924539352E-5 82-83 0.0 0.0 0.0 6.370248444887759 5.784042924539352E-5 84-85 0.0 0.0 0.0 6.79546236048527 5.784042924539352E-5 86-87 0.0 0.0 0.0 7.244745124702519 5.784042924539352E-5 88 0.0 0.0 0.0 7.598453809645412 5.784042924539352E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15880 0.0 47.373447 1 GTGGTAT 3645 0.0 45.9217 1 GTATCAA 27640 0.0 39.856556 1 GTACTAG 7615 0.0 38.590076 1 GGGCACC 7615 0.0 37.472717 94 TACCTGG 3095 0.0 34.92512 2 TAGGCAT 9465 0.0 34.302402 5 ACCTGGG 3115 0.0 34.100594 3 CAACGCA 32275 0.0 32.60985 5 ATCAACG 32530 0.0 32.350544 3 TATTCTC 3540 0.0 31.722084 5 CTAGGCA 10550 0.0 31.586779 4 TATCAAC 35590 0.0 31.018854 2 CTGTGCG 10345 0.0 30.975018 9 ACTGTGC 10960 0.0 30.180038 8 TAGTACT 10805 0.0 29.797329 4 AACGCAG 36900 0.0 29.248371 6 CCTGGGG 3125 0.0 29.17852 4 TACCCCG 440 0.0 28.832224 5 CTGGGGG 2745 0.0 28.585197 5 >>END_MODULE