FastQCFastQC Report
Mon 6 Jun 2016
ERR1211268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121406
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114130.537992256079223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89920.42386982972613446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54900.25879063225049803No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35680.16819034168848396No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.10101790982018528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG21950.060.459622
ACCTGGG24600.053.5059853
GGTATCA50350.050.190981
GTACACG2400.047.059881
GTACCTG29000.045.9239541
GTATCAA92000.042.4561961
CCTGGGG19000.038.844144
ATCAACG108750.035.1863823
CAACGCA109950.034.6313365
TATCAAC113150.034.062792
TCAACGC116050.032.9325144
AACGCAG118100.032.6004566
ACGCAGA127050.029.8599157
GTACATG92000.029.8216421
CGCAGAG127550.029.669158
CTGGGGG16600.029.4513175
TACATGG93100.028.7110792
TAACGCA3000.028.20534
ACATGGG91650.028.0052623
GTGGTAT22400.027.7317161
Produced by FastQC (version 0.11.5)