FastQCFastQC Report
Mon 6 Jun 2016
ERR1211268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121406
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103080.48590415978836676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84760.39954633860750843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53940.2542653315772653No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30230.1424998326581522No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21250.10016941594395415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG25050.057.7848472
ACCTGGG27500.051.1034743
GGTATCA48650.049.7737271
GTACCTG30950.046.9433021
TAACGCA2450.042.205454
CCTGGGG19750.040.6949844
GTATCAA85300.039.0265351
TATCACG1500.037.597672
GTACCCG1302.2919266E-1036.1686631
CTGGGGG14600.034.7559555
ATAACGC3150.032.826463
ATCAACG102750.031.9747583
CAACGCA104750.031.5774425
TATCAAC106400.031.1399882
TCAACGC109800.030.0073474
AACGCAG113500.029.6812116
GTACATG90950.028.8991371
GTGGTAT22600.028.0867251
TACATGG91600.027.8596272
ACGCAGA123400.027.1857557
Produced by FastQC (version 0.11.5)