##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211268_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2121406 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.179813765021876 33.0 31.0 34.0 30.0 34.0 2 32.29537250295323 34.0 31.0 34.0 30.0 34.0 3 32.37416882954041 34.0 31.0 34.0 30.0 34.0 4 35.98861038386806 37.0 35.0 37.0 35.0 37.0 5 35.895934111622196 37.0 35.0 37.0 35.0 37.0 6 35.928610553566834 37.0 35.0 37.0 35.0 37.0 7 35.884453046705815 37.0 35.0 37.0 35.0 37.0 8 35.87075976969991 37.0 35.0 37.0 35.0 37.0 9 37.482666684265055 39.0 37.0 39.0 35.0 39.0 10-11 37.46320553444272 39.0 37.0 39.0 35.0 39.0 12-13 37.37158846538569 39.0 37.0 39.0 34.0 39.0 14-15 38.58772719602 40.0 38.0 41.0 34.0 41.0 16-17 38.54205654174637 40.0 38.0 41.0 34.0 41.0 18-19 38.6064897525509 40.0 38.0 41.0 34.0 41.0 20-21 38.57436388885485 40.0 38.0 41.0 34.0 41.0 22-23 38.43289049809419 40.0 38.0 41.0 34.0 41.0 24-25 38.37180695255882 40.0 38.0 41.0 34.0 41.0 26-27 38.22786656585302 40.0 38.0 41.0 34.0 41.0 28-29 38.19365764969082 40.0 38.0 41.0 34.0 41.0 30-31 38.16511078030325 40.0 37.5 41.0 34.0 41.0 32-33 37.97550421748595 40.0 37.0 41.0 33.0 41.0 34-35 37.79559193289734 40.0 37.0 41.0 33.0 41.0 36-37 37.61744074448738 40.0 37.0 41.0 33.0 41.0 38-39 37.51681337754301 40.0 37.0 41.0 33.0 41.0 40-41 37.41809347197095 40.0 36.0 41.0 32.0 41.0 42-43 37.2640435164226 40.0 36.0 41.0 31.5 41.0 44-45 37.114127847286184 40.0 36.0 41.0 31.0 41.0 46-47 36.85443616167768 40.0 36.0 41.0 30.5 41.0 48-49 36.61059669860461 39.5 36.0 41.0 30.0 41.0 50-51 36.42313658960143 39.0 35.0 41.0 29.5 41.0 52-53 36.25531439997813 39.0 35.0 41.0 29.0 41.0 54-55 36.04805303652389 39.0 35.0 40.0 28.0 41.0 56-57 35.83188696553135 39.0 35.0 40.0 28.0 41.0 58-59 35.60486582954889 38.0 35.0 40.0 27.0 41.0 60-61 35.36453347449758 38.0 34.0 40.0 27.0 41.0 62-63 35.082029088255624 38.0 34.0 40.0 26.0 41.0 64-65 34.73041204748172 37.0 34.0 40.0 26.0 41.0 66-67 34.43873072858284 37.0 34.0 39.5 26.0 41.0 68-69 34.114177342762304 36.0 33.0 39.0 25.5 40.5 70-71 33.76593000114075 36.0 33.0 39.0 25.0 40.0 72-73 33.39905869032142 35.0 33.0 38.0 24.5 40.0 74-75 33.027348843172874 35.0 32.0 37.5 24.0 39.5 76-77 31.973983056520062 34.0 30.5 36.0 22.5 39.0 78-79 32.44055239779655 35.0 32.0 36.0 24.0 39.0 80-81 32.33078062379384 35.0 32.0 36.0 24.0 38.5 82-83 32.0335506263299 35.0 32.0 36.0 23.5 37.0 84-85 31.730010898432454 34.5 31.0 35.0 23.0 37.0 86-87 31.476657462079395 34.0 31.0 35.0 22.0 36.5 88-89 31.24176560262392 34.0 31.0 35.0 20.5 36.0 90-91 31.013032158860682 34.0 31.0 35.0 20.0 36.0 92-93 30.81456967690296 34.0 31.0 35.0 20.0 35.0 94-95 30.627707049004293 34.0 30.0 35.0 19.5 35.0 96-97 30.455975659539003 34.0 30.0 35.0 19.0 35.0 98-99 30.277101837177796 34.0 30.0 35.0 18.5 35.0 100 28.551176436759395 32.0 26.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23095847661792135 1101 2 -0.24613190066794033 1101 3 -0.37549913296657067 1101 4 -0.09411069983297438 1101 5 -0.1383492428604569 1101 6 -0.16942669646249442 1101 7 -0.18394074076217493 1101 8 -0.21335508148414561 1101 9 -0.3707953099448247 1101 10-11 -0.33692061005601204 1101 12-13 -0.3231347776218385 1101 14-15 -0.668363273883557 1101 16-17 -0.3629182060939016 1101 18-19 -0.5613310217960006 1101 20-21 -0.5292965740914397 1101 22-23 -0.6894331122097483 1101 24-25 -0.43476931679252573 1101 26-27 -0.6979920793343481 1101 28-29 -0.6681583688058694 1101 30-31 -0.4986468689650039 1101 32-33 -0.49664770417228965 1101 34-35 -0.4106342378623822 1101 36-37 -0.4015859328209004 1101 38-39 -0.4843276643742058 1101 40-41 -0.5978137505720582 1101 42-43 -0.5230281459696897 1101 44-45 -0.6891578215570746 1101 46-47 -0.3895341457360004 1101 48-49 -0.5060773746604781 1101 50-51 -0.6679533397198725 1101 52-53 -0.8147172579982325 1101 54-55 -0.6624410365327265 1101 56-57 -0.41463294118936744 1101 58-59 -0.5653288561315222 1101 60-61 -0.7764857949984858 1101 62-63 -0.721547393182469 1101 64-65 -0.6016331967228439 1101 66-67 -0.7697430940517407 1101 68-69 -0.6502450139169937 1101 70-71 -0.7995850411201886 1101 72-73 -0.7852282537164257 1101 74-75 -0.8663478270125111 1101 76-77 -0.686886815921163 1101 78-79 -0.593734988255207 1101 80-81 -0.5357791181361229 1101 82-83 -0.5081693126537736 1101 84-85 -0.35789804226014965 1101 86-87 -0.7352081352557356 1101 88-89 -0.6340905769466758 1101 90-91 -0.644400609749237 1101 92-93 -0.7802444490941696 1101 94-95 -0.7431639881492558 1101 96-97 -0.6779771796004113 1101 98-99 -0.8018688878877391 1101 100 -0.6953473356622197 1102 1 -0.3425239766687369 1102 2 -0.3013915483461602 1102 3 -0.3186891270694687 1102 4 -0.18570069554364466 1102 5 -0.29550704578466025 1102 6 -0.2600230273962225 1102 7 -0.26405148227554776 1102 8 -0.31110545428787617 1102 9 -0.41958192495584967 1102 10-11 -0.507944788179536 1102 12-13 -0.41716472135532 1102 14-15 -0.43425301011020423 1102 16-17 -0.5696093799045698 1102 18-19 -0.4669127707493672 1102 20-21 -0.5294475904660985 1102 22-23 -0.5424156568988252 1102 24-25 -0.570177029422716 1102 26-27 -0.6417179518845657 1102 28-29 -0.751689372812109 1102 30-31 -0.5222723184971159 1102 32-33 -0.589228493626166 1102 34-35 -0.6211084292787987 1102 36-37 -0.6627709060599969 1102 38-39 -0.6978951075406599 1102 40-41 -0.5219082121757097 1102 42-43 -0.5004638305073996 1102 44-45 -0.3962647210530932 1102 46-47 -0.48982251236471086 1102 48-49 -0.5488711373971 1102 50-51 -0.5407791776454189 1102 52-53 -0.5363525930737083 1102 54-55 -0.39379970157094135 1102 56-57 -0.5380268131401138 1102 58-59 -0.7161412438382158 1102 60-61 -0.6251350884657967 1102 62-63 -0.832899862529878 1102 64-65 -0.6784059308599524 1102 66-67 -0.6275377783901916 1102 68-69 -0.6839457821482 1102 70-71 -0.5757220709668829 1102 72-73 -0.6613721453337504 1102 74-75 -0.7579004131535712 1102 76-77 -0.4591045976112049 1102 78-79 -0.2437891359515163 1102 80-81 -0.37824535334383924 1102 82-83 -0.3836357838604627 1102 84-85 -0.5114033160620259 1102 86-87 -0.5363234457321937 1102 88-89 -0.4917890816548862 1102 90-91 -0.5311216767631848 1102 92-93 -0.5519685565328452 1102 94-95 -0.7644557179068876 1102 96-97 -0.9089673474582725 1102 98-99 -0.6509179728031533 1102 100 -0.8327219935067767 1103 1 -0.3473134013223458 1103 2 -0.3682411590631425 1103 3 -0.3729754555847151 1103 4 -0.22406330414032993 1103 5 -0.27985226205066027 1103 6 -0.3343421509509028 1103 7 -0.4223596291872056 1103 8 -0.41775930644041637 1103 9 -0.6185710958483526 1103 10-11 -0.5724991676662015 1103 12-13 -0.6105694618824842 1103 14-15 -0.726274288948126 1103 16-17 -0.7633804300624263 1103 18-19 -0.7003401052882694 1103 20-21 -0.6657552399722775 1103 22-23 -0.6457541750520122 1103 24-25 -0.8345240161150116 1103 26-27 -0.7856548741249156 1103 28-29 -0.8395029384928137 1103 30-31 -0.8039526216615442 1103 32-33 -0.8253721679351784 1103 34-35 -0.8398327949814188 1103 36-37 -0.7159144665558799 1103 38-39 -0.7762255112400069 1103 40-41 -0.8275971819983781 1103 42-43 -0.7825003265808448 1103 44-45 -0.6589942968673839 1103 46-47 -0.5957108659104122 1103 48-49 -0.842299062078105 1103 50-51 -0.916718241258522 1103 52-53 -0.7565628522725447 1103 54-55 -0.7845062281210176 1103 56-57 -0.8736625437149499 1103 58-59 -0.842396244238671 1103 60-61 -0.8642113474707358 1103 62-63 -0.9582270752465973 1103 64-65 -0.8633143112992769 1103 66-67 -0.9699323221049738 1103 68-69 -0.8516262286328455 1103 70-71 -1.0878494910763905 1103 72-73 -0.7207943962200218 1103 74-75 -0.715150519589919 1103 76-77 -0.775447318419193 1103 78-79 -0.6240151603749666 1103 80-81 -0.6380895558300246 1103 82-83 -0.8276262404196828 1103 84-85 -0.6455358704077874 1103 86-87 -0.6405800563775159 1103 88-89 -0.877054076533291 1103 90-91 -0.7930650989972108 1103 92-93 -0.938118764557931 1103 94-95 -0.8539145154502101 1103 96-97 -0.9091688388316612 1103 98-99 -1.0462996893579053 1103 100 -0.9633491037916855 1104 1 -0.3271035344298525 1104 2 -0.33230240371604225 1104 3 -0.3474217457286777 1104 4 -0.3239680334739248 1104 5 -0.3322937425188215 1104 6 -0.3649856486600598 1104 7 -0.4048800404524826 1104 8 -0.3636832961913541 1104 9 -0.4803523587411078 1104 10-11 -0.5151326551697295 1104 12-13 -0.515058854538772 1104 14-15 -0.6135054410989156 1104 16-17 -0.6350147427022179 1104 18-19 -0.831158673025449 1104 20-21 -0.7831136368193867 1104 22-23 -0.6995185877938539 1104 24-25 -0.7354745513678722 1104 26-27 -0.8479622970941065 1104 28-29 -0.8546209818341879 1104 30-31 -0.7848436602475175 1104 32-33 -0.8333306614100877 1104 34-35 -0.6588855711306394 1104 36-37 -0.7752185564088805 1104 38-39 -0.8260245199907956 1104 40-41 -0.7905505997996087 1104 42-43 -0.6308151757763341 1104 44-45 -0.6949714590500946 1104 46-47 -0.8966311371196696 1104 48-49 -0.9580256434083125 1104 50-51 -0.8214696961040318 1104 52-53 -0.8541235432155858 1104 54-55 -0.809813211379435 1104 56-57 -0.7770115383515659 1104 58-59 -0.8235561306738646 1104 60-61 -0.8096059200284387 1104 62-63 -0.8474281044955063 1104 64-65 -0.7945586113448684 1104 66-67 -0.9025272399170561 1104 68-69 -1.0879144454699698 1104 70-71 -1.1613768671430975 1104 72-73 -1.1572461474631481 1104 74-75 -0.9499693696105425 1104 76-77 -0.9960580821045077 1104 78-79 -0.7506821256106768 1104 80-81 -0.8431897935995494 1104 82-83 -0.8049385219214322 1104 84-85 -0.7173760599719863 1104 86-87 -0.8306291574132771 1104 88-89 -1.110402881171428 1104 90-91 -1.0897615682825972 1104 92-93 -1.1016777346576028 1104 94-95 -0.9927671689146962 1104 96-97 -1.0409173134152923 1104 98-99 -1.2175820976700642 1104 100 -1.1505486347668672 1105 1 -0.3822605028776067 1105 2 -0.33866625726357924 1105 3 -0.4279837767624066 1105 4 -0.311828208140426 1105 5 -0.2651780002504722 1105 6 -0.3352637394986431 1105 7 -0.37398584952777014 1105 8 -0.3789374218089705 1105 9 -0.3978209907630159 1105 10-11 -0.5016148564133047 1105 12-13 -0.5469935560073225 1105 14-15 -0.6937531763480322 1105 16-17 -0.6858050486257028 1105 18-19 -0.681339885234955 1105 20-21 -0.7000989737894585 1105 22-23 -0.7062145476668462 1105 24-25 -0.6924116097200255 1105 26-27 -0.7367172572627751 1105 28-29 -0.6361931785354074 1105 30-31 -0.5713930326736971 1105 32-33 -0.7613581319597529 1105 34-35 -0.7381419422155346 1105 36-37 -0.6317525073171382 1105 38-39 -0.6688041802995883 1105 40-41 -0.6776482422732215 1105 42-43 -0.7369840775542826 1105 44-45 -0.765095307926714 1105 46-47 -0.6531164505209048 1105 48-49 -0.790837025227745 1105 50-51 -0.800552755896021 1105 52-53 -0.8183623876569825 1105 54-55 -0.8703693180648386 1105 56-57 -0.7722266629021561 1105 58-59 -1.0340966765709538 1105 60-61 -1.0184066573044745 1105 62-63 -0.937475520708297 1105 64-65 -0.9324393819601013 1105 66-67 -0.9245114734370858 1105 68-69 -0.8823263012372635 1105 70-71 -0.8043305618795387 1105 72-73 -0.8969864724559713 1105 74-75 -0.9720557793911837 1105 76-77 -0.8276241991173521 1105 78-79 -0.9200701447142876 1105 80-81 -0.8809991613548114 1105 82-83 -0.8072388938805126 1105 84-85 -0.8072805901165303 1105 86-87 -0.7335594332972164 1105 88-89 -0.7682929239810043 1105 90-91 -0.8625957529508561 1105 92-93 -0.6569451820241063 1105 94-95 -0.7211027216467549 1105 96-97 -0.7825945682029811 1105 98-99 -0.9565962079036545 1105 100 -1.1836757050748474 1106 1 -0.45970249644130945 1106 2 -0.40863963925670177 1106 3 -0.4737714651291185 1106 4 -0.33736572223615724 1106 5 -0.3253521419104004 1106 6 -0.3568596483842654 1106 7 -0.39876947837584 1106 8 -0.4683682955018753 1106 9 -0.5667080223392205 1106 10-11 -0.5383348748940122 1106 12-13 -0.563862911218628 1106 14-15 -0.7059281821993366 1106 16-17 -0.73240608611178 1106 18-19 -0.6324879690402199 1106 20-21 -0.7333154418079104 1106 22-23 -0.7070472954479783 1106 24-25 -0.7418058009238848 1106 26-27 -0.7037884726662682 1106 28-29 -0.8168109485717565 1106 30-31 -0.783454765554886 1106 32-33 -0.7536436772181005 1106 34-35 -0.8676682015373558 1106 36-37 -0.7418839085268516 1106 38-39 -0.7651394561193996 1106 40-41 -0.670618446182317 1106 42-43 -0.5944851850530455 1106 44-45 -0.8046522222056751 1106 46-47 -0.8621588387522863 1106 48-49 -0.731816406605688 1106 50-51 -0.8904810214198378 1106 52-53 -0.8775198112205587 1106 54-55 -0.8500245594691407 1106 56-57 -0.7020946683216138 1106 58-59 -0.8142484730011432 1106 60-61 -0.6122443237240773 1106 62-63 -0.7231980295714564 1106 64-65 -0.6090229910798541 1106 66-67 -0.7173342728744458 1106 68-69 -0.5966519720569394 1106 70-71 -0.6969317094181591 1106 72-73 -0.6829250291600388 1106 74-75 -0.7409725809398324 1106 76-77 -0.7690829683394611 1106 78-79 -0.5373351819102723 1106 80-81 -0.6722913683418312 1106 82-83 -0.6783425205173863 1106 84-85 -0.7037309139473891 1106 86-87 -0.6012822408744114 1106 88-89 -0.5630631969138378 1106 90-91 -0.7148715202693623 1106 92-93 -0.656552264031756 1106 94-95 -0.6762620176249428 1106 96-97 -0.8235986480078026 1106 98-99 -0.8769608976734098 1106 100 -0.942551860721597 1107 1 -0.39412034115254 1107 2 -0.4424693652374927 1107 3 -0.494476883573288 1107 4 -0.2521801077773347 1107 5 -0.2869358625247145 1107 6 -0.3730241272093764 1107 7 -0.4678260083133168 1107 8 -0.37319052013356213 1107 9 -0.559780709028594 1107 10-11 -0.6493210673767109 1107 12-13 -0.6034489474180305 1107 14-15 -0.7256875507848903 1107 16-17 -0.7736075482454865 1107 18-19 -0.8019293497171773 1107 20-21 -0.8383961445593187 1107 22-23 -0.7790059090381405 1107 24-25 -0.8404244260545894 1107 26-27 -0.7874092600898237 1107 28-29 -0.7802890122042285 1107 30-31 -0.8509169316613523 1107 32-33 -0.8346667221501747 1107 34-35 -0.9926003728407977 1107 36-37 -1.0246961105884438 1107 38-39 -0.8977949386845836 1107 40-41 -0.913330896128663 1107 42-43 -0.8886610772948416 1107 44-45 -0.8939440744120191 1107 46-47 -0.9579692249863925 1107 48-49 -0.8622909021433927 1107 50-51 -0.8760390238351263 1107 52-53 -0.8210574220302718 1107 54-55 -0.811200871706717 1107 56-57 -0.8555028032785899 1107 58-59 -0.9137568162112331 1107 60-61 -0.8096252698736421 1107 62-63 -0.9055255540016205 1107 64-65 -0.8391434189521263 1107 66-67 -0.6315437149281067 1107 68-69 -0.8505498943863756 1107 70-71 -0.9421421995918706 1107 72-73 -0.9279615388828759 1107 74-75 -0.7531777582291497 1107 76-77 -0.8532212105137695 1107 78-79 -0.7496528981302077 1107 80-81 -0.6128870145932126 1107 82-83 -0.7369615943082799 1107 84-85 -0.6725627399071179 1107 86-87 -0.7266790247286252 1107 88-89 -0.6639282386828533 1107 90-91 -0.6481446583607351 1107 92-93 -0.7389114439307853 1107 94-95 -0.6941760219295858 1107 96-97 -0.8975681316373176 1107 98-99 -0.8372479903429237 1107 100 -1.0837197979689925 1108 1 -0.42952805135058014 1108 2 -0.3669572005138946 1108 3 -0.44204610262486455 1108 4 -0.36693913163198744 1108 5 -0.3054411786022797 1108 6 -0.33552361717751467 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0.6883346825083478 2311 22-23 0.8652923365758838 2311 24-25 0.8605540200607038 2311 26-27 0.19487845920841806 2311 28-29 0.5235601106027872 2311 30-31 0.7414840708449191 2311 32-33 0.8215172377495747 2311 34-35 0.9151119078609469 2311 36-37 0.9621625360280959 2311 38-39 1.034791026227694 2311 40-41 0.9618901565029105 2311 42-43 1.0605213788455146 2311 44-45 1.076125599773441 2311 46-47 0.7119986948131043 2311 48-49 0.8407722557513537 2311 50-51 0.9757324047363056 2311 52-53 0.8139260366163441 2311 54-55 0.9654968726541853 2311 56-57 1.065882159127419 2311 58-59 1.1375980710068063 2311 60-61 1.1238423992992992 2311 62-63 0.9929651728154241 2311 64-65 1.146967859243361 2311 66-67 1.2107555331921915 2311 68-69 1.2570952183955768 2311 70-71 1.2756512841174015 2311 72-73 1.1865647769066001 2311 74-75 1.2277104623222286 2311 76-77 1.1949041027694385 2311 78-79 0.8791300592632751 2311 80-81 0.7310282736273379 2311 82-83 0.843973242784454 2311 84-85 0.9738873854061687 2311 86-87 1.0542292459480649 2311 88-89 1.0900466986605082 2311 90-91 1.0348728854989133 2311 92-93 1.1548579796281153 2311 94-95 1.155990394110514 2311 96-97 1.2164213420468926 2311 98-99 1.156935969556823 2311 100 1.2827253148129643 2312 1 0.17461084662240722 2312 2 0.2794192298320297 2312 3 0.3851841815693078 2312 4 0.15815184040755526 2312 5 0.2983133220249883 2312 6 0.4170564226255067 2312 7 0.29143701959682033 2312 8 0.4190150748912771 2312 9 0.6169494063733652 2312 10-11 0.6424865513459395 2312 12-13 0.7246344295820109 2312 14-15 0.93665722931771 2312 16-17 0.8651929578820656 2312 18-19 0.7728439896279511 2312 20-21 0.8746072919086672 2312 22-23 0.9302031955710248 2312 24-25 0.8762700654415738 2312 26-27 0.9868599827092268 2312 28-29 0.9960537896951678 2312 30-31 0.9522312345402142 2312 32-33 0.8260242069554096 2312 34-35 0.9615339500003373 2312 36-37 0.9990785814089733 2312 38-39 0.8191518041855517 2312 40-41 1.093704419063691 2312 42-43 1.1039567110983484 2312 44-45 0.755384270762093 2312 46-47 0.8145471551048402 2312 48-49 1.0207680418129357 2312 50-51 0.8553281907978914 2312 52-53 1.0411996184639989 2312 54-55 0.9397688337562897 2312 56-57 0.8928581720933906 2312 58-59 1.047817195804214 2312 60-61 1.1009513133997828 2312 62-63 1.195124978325957 2312 64-65 1.114354893279014 2312 66-67 1.0229529399993211 2312 68-69 1.1524628034847169 2312 70-71 1.0445867784447884 2312 72-73 0.9305388449779102 2312 74-75 0.9247522775572392 2312 76-77 0.982033762412879 2312 78-79 0.8358477253896908 2312 80-81 0.7472072039352824 2312 82-83 0.7659035507261045 2312 84-85 0.8223658294904475 2312 86-87 0.9171316770663793 2312 88-89 0.9443611232958347 2312 90-91 0.9563607299073418 2312 92-93 1.0016375582342718 2312 94-95 0.9528523065902519 2312 96-97 0.6482051345914641 2312 98-99 0.4648935060235999 2312 100 0.7073399015228503 2313 1 0.023373533142290626 2313 2 0.2340406173376124 2313 3 0.2532036460315368 2313 4 0.19661739455448668 2313 5 0.3488182615169393 2313 6 0.2405444971836772 2313 7 0.22181332736133186 2313 8 0.41983692282564533 2313 9 0.5715370148783023 2313 10-11 0.3974214370694824 2313 12-13 0.4320279578487316 2313 14-15 0.5709039479059896 2313 16-17 0.7724221650485177 2313 18-19 0.7131850217719844 2313 20-21 0.6772214794768345 2313 22-23 0.6662197398467313 2313 24-25 0.5711454839617716 2313 26-27 0.7182408844178312 2313 28-29 0.6001419215321846 2313 30-31 0.882360018192351 2313 32-33 0.9539232209964865 2313 34-35 1.0140350829706506 2313 36-37 1.0415307849311688 2313 38-39 1.0280708826297627 2313 40-41 1.0521783096740336 2313 42-43 1.0075523321761892 2313 44-45 0.9307903783933043 2313 46-47 0.9098369078166968 2313 48-49 0.9828605684757221 2313 50-51 0.9040282164634732 2313 52-53 0.96116528671606 2313 54-55 0.7911597366350378 2313 56-57 0.6642898974600868 2313 58-59 0.7246799218245172 2313 60-61 0.6751607878114569 2313 62-63 1.0468640958309763 2313 64-65 1.1675218281304751 2313 66-67 1.173285170208537 2313 68-69 1.1443998853281627 2313 70-71 1.094270031624383 2313 72-73 1.1053808917849395 2313 74-75 1.0900322812292913 2313 76-77 1.049038127500193 2313 78-79 0.9869125881822995 2313 80-81 0.7946562752228701 2313 82-83 0.85408532894321 2313 84-85 0.856861138896388 2313 86-87 0.8505183564386982 2313 88-89 0.763023164555996 2313 90-91 0.8915414136201747 2313 92-93 1.0305253110999892 2313 94-95 1.211209700053871 2313 96-97 1.2079700456767313 2313 98-99 1.2087811231268297 2313 100 1.0983460567355792 2314 1 -0.3674794145116138 2314 2 0.08754485951357083 2314 3 0.3569218836149517 2314 4 0.22136185523831386 2314 5 0.37091145848049933 2314 6 0.4193148282874759 2314 7 0.4519193009056792 2314 8 0.46453893864852347 2314 9 0.5625927922922784 2314 10-11 0.6484944172808724 2314 12-13 0.5551773480503712 2314 14-15 0.897417509162274 2314 16-17 0.9268124205955388 2314 18-19 0.896688817322044 2314 20-21 0.8248227133963795 2314 22-23 0.9074738037573624 2314 24-25 0.8607609698676484 2314 26-27 0.7108058723358326 2314 28-29 0.7573523885950664 2314 30-31 0.8428817542426046 2314 32-33 0.9809635697959038 2314 34-35 0.9364045719536165 2314 36-37 1.0330868603524195 2314 38-39 0.6272473968840586 2314 40-41 0.6674453688581323 2314 42-43 0.862043386567521 2314 44-45 0.9359719318197719 2314 46-47 0.9342952199096288 2314 48-49 0.9400765028555966 2314 50-51 1.0751192773231821 2314 52-53 1.0680403223306314 2314 54-55 0.9522211969785062 2314 56-57 0.9102968309420945 2314 58-59 1.0241826269913616 2314 60-61 1.0981373799942773 2314 62-63 1.0312284932787392 2314 64-65 1.0342867781622829 2314 66-67 0.7300088150454727 2314 68-69 0.7319739828743437 2314 70-71 0.8260620949282114 2314 72-73 1.0169277112695312 2314 74-75 1.1242726244843908 2314 76-77 0.9202631761285112 2314 78-79 0.7945725303057429 2314 80-81 0.8729966133956779 2314 82-83 0.9618767176879253 2314 84-85 0.9554024433212227 2314 86-87 1.121339670658486 2314 88-89 0.9660080886218907 2314 90-91 1.1754752021012322 2314 92-93 1.1036070143771468 2314 94-95 0.9783363400564582 2314 96-97 1.0892638130893673 2314 98-99 1.1081120313328867 2314 100 1.0339392144268622 2315 1 0.3830539378284925 2315 2 0.3707278067970705 2315 3 0.38135448589515875 2315 4 0.25118651774168654 2315 5 0.4056313764682855 2315 6 0.39347794887635956 2315 7 0.3558151842202548 2315 8 0.25291953111395316 2315 9 0.4556384358485275 2315 10-11 0.46406688010392116 2315 12-13 0.32159874644349173 2315 14-15 0.6071651032382235 2315 16-17 0.7435697232915643 2315 18-19 0.7513867764190607 2315 20-21 0.5002190501438477 2315 22-23 0.7259168456096674 2315 24-25 0.8014881913097156 2315 26-27 0.8565158511958444 2315 28-29 0.7968777853867621 2315 30-31 0.8303403159352527 2315 32-33 0.6491921788336299 2315 34-35 0.6701909337839709 2315 36-37 0.7089589225167074 2315 38-39 0.8040380018757673 2315 40-41 0.7642810441698416 2315 42-43 0.8795374039829866 2315 44-45 0.852197398673681 2315 46-47 0.7875569037683263 2315 48-49 0.7296965291213482 2315 50-51 0.7909848792060643 2315 52-53 0.8250597365377885 2315 54-55 0.6345347359691829 2315 56-57 0.6489299203214571 2315 58-59 0.6392507025857554 2315 60-61 0.8515743710196375 2315 62-63 0.8813464768216974 2315 64-65 0.8651714727547528 2315 66-67 0.981619790851262 2315 68-69 0.9855627753476597 2315 70-71 0.9768773642430872 2315 72-73 0.9602552718005484 2315 74-75 0.3635637223379362 2315 76-77 0.37145241384564187 2315 78-79 0.6698772705751423 2315 80-81 0.6994497975864888 2315 82-83 0.6982065499329231 2315 84-85 0.7551857357596639 2315 86-87 0.7531492773699 2315 88-89 0.8826060073800619 2315 90-91 0.8395091777220074 2315 92-93 0.869800634773437 2315 94-95 0.8198609361372213 2315 96-97 1.0096196138457927 2315 98-99 0.9823139742701024 2315 100 0.7330684412858623 2316 1 0.322712438470937 2316 2 0.09274075308155716 2316 3 0.2041563273610194 2316 4 0.019347548572845596 2316 5 0.268726549318842 2316 6 0.36746663457881823 2316 7 0.4589065206835983 2316 8 0.41299004756474744 2316 9 0.6028759325754152 2316 10-11 0.6034052250986619 2316 12-13 0.3306450084017527 2316 14-15 0.49846731311905756 2316 16-17 0.750637657792872 2316 18-19 0.6132903856678027 2316 20-21 0.5949036747564094 2316 22-23 0.6393233443278277 2316 24-25 0.27878347742503706 2316 26-27 0.5482598545572515 2316 28-29 0.7557166997500744 2316 30-31 0.40618949720150255 2316 32-33 0.6619017338736057 2316 34-35 0.7608777993338194 2316 36-37 0.8035299778885658 2316 38-39 0.7124049797160694 2316 40-41 0.6043149627044642 2316 42-43 0.6403678904734207 2316 44-45 0.5081891656649162 2316 46-47 0.6795475320224043 2316 48-49 0.8237862092397208 2316 50-51 0.8393541101626596 2316 52-53 0.9268996800271978 2316 54-55 0.7782844695534052 2316 56-57 0.8687147172669611 2316 58-59 0.6798259779835547 2316 60-61 0.9567834845706145 2316 62-63 0.9661915294045613 2316 64-65 0.9723446034294057 2316 66-67 0.8097834401689354 2316 68-69 0.9634254509537143 2316 70-71 0.9061427569856235 2316 72-73 0.9290764048135713 2316 74-75 0.9088158886788236 2316 76-77 0.8507800717616867 2316 78-79 0.5934912995733583 2316 80-81 0.544080986805632 2316 82-83 0.5179809947224321 2316 84-85 0.6430300210650834 2316 86-87 0.5427532769558177 2316 88-89 0.6334110397457771 2316 90-91 0.4346480792973608 2316 92-93 0.24424557652733725 2316 94-95 0.3081655879089631 2316 96-97 0.5949655280934039 2316 98-99 0.866684806766127 2316 100 0.3831625241152885 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 10.0 13 67.0 14 269.0 15 833.0 16 2293.0 17 5010.0 18 8249.0 19 11161.0 20 13997.0 21 18324.0 22 21689.0 23 20811.0 24 18703.0 25 18624.0 26 20775.0 27 24424.0 28 29926.0 29 36440.0 30 44855.0 31 55705.0 32 70362.0 33 91753.0 34 130547.0 35 180533.0 36 277060.0 37 459225.0 38 479581.0 39 80160.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.139922415183456 19.751871948269244 13.18611773161086 22.922087904936436 2 15.416096683048883 21.31850291740478 41.81571090116649 21.449689498379847 3 18.444418465866505 28.324846823286066 30.582500473742414 22.648234237105015 4 11.519713826155803 17.789291963998153 40.516931213687755 30.174062996158284 5 11.618756617073771 37.997535596675036 34.75190510444488 15.631802681806311 6 26.858932236450734 37.58578037395953 21.316900206749676 14.238387182840059 7 25.537136281936036 33.39510103993186 23.728462813750802 17.339299864381307 8 25.468910712989405 34.80767000753274 21.97716985810354 17.746249421374316 9 26.597833066340304 16.881376835439575 22.175146170433642 34.345643927786476 10-11 24.095237174910796 26.73656081118187 29.141224527122084 20.02697748678525 12-13 24.921160777333522 25.084283725038958 28.91643560921389 21.078119888413628 14-15 22.357250754040898 26.2618591889756 28.126803788152515 23.25408626883099 16-17 20.753906730592913 28.284064838222626 29.115967121758572 21.846061309425885 18-19 19.93425586615669 27.481703172330047 31.64422557492531 20.939815386587952 20-21 21.61508019093945 27.07883994832671 31.069048326687188 20.23703153404665 22-23 21.127356102509374 27.299441973860734 30.606470425745947 20.96673149788395 24-25 20.414463515246823 27.149472068588892 31.60362082838965 20.832443587774637 26-27 20.355789217150118 27.321766581589063 31.18019191603267 21.14225228522815 28-29 20.026011112467657 27.833351638846217 31.27871628230589 20.861920966380236 30-31 21.22682523209828 27.20135988650642 31.304400704550183 20.267414176845115 32-33 20.911827965100436 27.396068377196663 31.019000894836445 20.673102762866456 34-35 21.050905574240772 27.68177143722042 31.10940211966283 20.15792086887598 36-37 20.982723230768578 27.72601049931886 30.452716520748485 20.838549749164077 38-39 20.6205002919749 28.043593726535626 30.714486269164315 20.62141971232516 40-41 21.296960391972096 27.04792244465914 31.145264004416003 20.509853158952765 42-43 20.945217687047574 27.249316549806245 30.451108946403693 21.354356816742484 44-45 20.665750656069605 27.986386658774737 30.585042249397677 20.76282043575798 46-47 20.65988989733951 27.364964176442424 29.96885225767502 22.00629366854304 48-49 20.566610444182764 27.43193669643724 30.35693577720434 21.64451708217565 50-51 20.873075813377234 27.84715032746295 29.835712224843437 21.444061634316384 52-53 20.582627255980494 28.44457120901579 29.39899842893124 21.573803106072482 54-55 20.646257910098196 28.133148467763945 29.5985113953029 21.62208222683496 56-57 21.10315068399519 28.069811560450862 29.2449101334899 21.58212762206405 58-59 20.496354466709345 28.299483411951392 29.781998712065572 21.422163409273693 60-61 21.101739798808143 28.750556555069668 29.13039286836403 21.017310777758162 62-63 20.953201398619527 28.21673653086261 29.359625997730248 21.47043607278761 64-65 20.58813751423824 27.881606679589048 29.265454106472692 22.264801699700016 66-67 20.540511984543862 27.901516180643604 29.058721812171704 22.499250022640826 68-69 20.309137455087455 28.059412502048847 29.365770597273468 22.265679445590226 70-71 20.623356765357236 27.657538875786376 29.47449742282324 22.244606936033147 72-73 20.63572612153915 27.82217891758476 29.274065927380416 22.26802903349567 74-75 20.849158033225976 27.912625021809557 28.78705856278559 22.451158382178882 76-77 20.387996318224598 27.802119661472823 29.142539595117878 22.6673444251847 78-79 20.14407788503471 27.75055101776222 29.228056527939845 22.877314569263227 80-81 19.970594599079185 28.08086155279184 28.96670043401014 22.981843414118835 82-83 20.771358657182994 27.881353814115815 29.223925600679447 22.123361928021744 84-85 20.913082921806865 28.006517136016328 28.60726992229478 22.473130019882028 86-87 20.034747762703013 28.116255722214113 29.232245187986717 22.616751327096157 88-89 20.50404339497056 27.444304551632005 29.085295986512428 22.966356066885012 90-91 20.862729321723187 27.438477707940606 29.148636838905663 22.550156131430548 92-93 20.62530195678608 27.765263952378245 29.105722188674743 22.50371190216093 94-95 19.797122671174947 27.69077766892615 29.3955968835538 23.116502776345104 96-97 19.912550269099665 27.83306534146218 29.06943417181555 23.1849502176226 98-99 19.751038889977266 27.780744294798392 28.954377011386946 23.513839803837392 100 19.900004856880006 27.511773218597796 28.95865191872695 23.629570005795248 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 807.0 1 558.5 2 995.5 3 1484.0 4 936.5 5 631.0 6 854.5 7 1423.0 8 2097.0 9 2459.0 10 2331.0 11 2086.5 12 2195.0 13 2517.0 14 2904.5 15 3288.5 16 3618.0 17 3991.0 18 4432.0 19 4796.5 20 5285.0 21 5867.5 22 6571.5 23 7522.0 24 8571.5 25 10189.5 26 12604.5 27 15218.0 28 17728.5 29 21387.5 30 27137.5 31 33183.0 32 40374.5 33 48847.5 34 57300.0 35 64881.5 36 71934.5 37 80120.5 38 82760.0 39 80247.5 40 77017.5 41 73344.0 42 71046.5 43 70799.0 44 72854.0 45 77501.5 46 80302.0 47 80391.0 48 80547.0 49 79271.5 50 78116.5 51 76851.5 52 73923.0 53 69149.0 54 63448.0 55 56064.5 56 48170.0 57 39569.0 58 30867.0 59 24562.5 60 18079.5 61 12166.5 62 8223.0 63 5889.0 64 4059.0 65 2259.0 66 1397.0 67 972.0 68 744.0 69 595.0 70 434.5 71 312.5 72 215.0 73 153.0 74 134.0 75 96.5 76 53.5 77 31.0 78 21.0 79 14.0 80 9.5 81 8.0 82 4.0 83 2.0 84 1.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04185903122740296 2 0.0 3 0.0 4 0.03474111037679727 5 0.0 6 0.0 7 8.013553275516332E-4 8 0.0 9 0.011878914267235974 10-11 3.535391150963088E-4 12-13 0.0 14-15 1.1784637169876958E-4 16-17 1.414156460385235E-4 18-19 0.0 20-21 2.3569274339753918E-5 22-23 0.0 24-25 6.128011328336019E-4 26-27 4.4781621245532446E-4 28-29 2.3569274339753916E-4 30-31 0.0024747738056741615 32-33 0.016404214940468724 34-35 0.028141713561666178 36-37 0.03285556842961696 38-39 0.023781397808811704 40-41 0.0014377257347249891 42-43 2.3569274339753918E-5 44-45 0.01185534499289622 46-47 0.01817191051595027 48-49 0.003393975504924564 50-51 0.011784637169876959 52-53 0.011242543860062619 54-55 0.010606173452889263 56-57 4.4781621245532446E-4 58-59 0.008484938762311411 60-61 0.004596008496252014 62-63 0.0013434486373659733 64-65 0.03754585402322799 66-67 0.06325993232789952 68-69 0.06191648369053355 70-71 0.05465714719388934 72-73 0.0484348587681943 74-75 0.036579513775298084 76-77 0.03252559858886041 78-79 0.015602859612917094 80-81 0.0012727408143467114 82-83 0.04002062782890215 84-85 0.05418576170709426 86-87 0.0598423875486352 88-89 0.07207484093096748 90-91 0.050768216927829936 92-93 0.040656998236075506 94-95 0.012821685240826131 96-97 0.0034411140536040716 98-99 0.024158506198247765 100 0.03313839972169401 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2121406.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.64787834457061 #Duplication Level Percentage of deduplicated Percentage of total 1 89.05937027593326 70.04330518905668 2 8.477854700505356 13.335305702165826 3 1.3982351841304197 3.299046919757626 4 0.3920030122505904 1.2332082087275864 5 0.17139180439852378 0.6739800890795771 6 0.09841180592713894 0.46439278441362714 7 0.06272933787417113 0.34534705336342947 8 0.03964986783595275 0.2494702385552252 9 0.0286341461850913 0.20268133610991404 >10 0.21766182201807105 3.4224432454113725 >50 0.03212155380344918 1.7648079311801452 >100 0.02049620175222412 2.8723344403553965 >500 9.39317860195707E-4 0.4348303500986244 >1k 3.131059533985198E-4 0.4680742606565588 >5k 1.252423813594079E-4 0.683100799169452 >10k+ 6.262119067970396E-5 0.5076714518989698 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10308 0.48590415978836676 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8476 0.39954633860750843 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5394 0.2542653315772653 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3023 0.1424998326581522 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2125 0.10016941594395415 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.7138548679507836E-5 0.0 0.0 0.0 6 0.0 4.7138548679507836E-5 0.0 0.0 0.0 7 0.0 4.7138548679507836E-5 0.0 0.0 0.0 8 0.0 4.7138548679507836E-5 0.0 0.0 0.0 9 0.0 4.7138548679507836E-5 0.0 0.0 0.0 10-11 0.0 4.7138548679507836E-5 0.0 0.0 0.0 12-13 0.0 4.7138548679507836E-5 0.0 2.3569274339753918E-5 0.0 14-15 0.0 4.7138548679507836E-5 0.0 4.7138548679507836E-5 0.0 16-17 0.0 4.7138548679507836E-5 0.0 9.427709735901567E-5 0.0 18-19 0.0 4.7138548679507836E-5 0.0 2.1212346905778528E-4 0.0 20-21 0.0 4.7138548679507836E-5 0.0 5.892318584938479E-4 0.0 22-23 0.0 4.7138548679507836E-5 0.0 0.0013670179117057273 0.0 24-25 0.0 4.7138548679507836E-5 0.0 0.0031347134871872713 0.0 26-27 0.0 4.7138548679507836E-5 0.0 0.005609487292861433 0.0 28-29 0.0 4.7138548679507836E-5 0.0 0.010841866196286801 0.0 30-31 0.0 7.070782301926175E-5 0.0 0.029084484535256333 0.0 32-33 0.0 9.427709735901567E-5 0.0 0.07009502188642815 4.7138548679507836E-5 34-35 0.0 9.427709735901567E-5 0.0 0.13007882508110186 4.7138548679507836E-5 36-37 0.0 9.427709735901567E-5 0.0 0.22322459727180935 4.7138548679507836E-5 38-39 0.0 9.427709735901567E-5 0.0 0.3667143394522312 4.7138548679507836E-5 40-41 0.0 9.427709735901567E-5 0.0 0.5390764426988516 4.7138548679507836E-5 42-43 0.0 9.427709735901567E-5 0.0 0.7034485619442954 4.7138548679507836E-5 44-45 0.0 9.427709735901567E-5 0.0 0.8872181939713567 4.7138548679507836E-5 46-47 0.0 9.427709735901567E-5 0.0 1.084681574389815 4.7138548679507836E-5 48-49 0.0 1.1784637169876959E-4 0.0 1.3010003742800764 1.414156460385235E-4 50-51 0.0 1.414156460385235E-4 0.0 1.5265347604371817 1.414156460385235E-4 52-53 0.0 1.414156460385235E-4 0.0 1.7495708035142732 1.649849203782774E-4 54-55 0.0 1.414156460385235E-4 0.0 2.014418739270088 1.8855419471803134E-4 56-57 0.0 1.414156460385235E-4 0.0 2.312004397083821 1.8855419471803134E-4 58-59 0.0 1.414156460385235E-4 0.0 2.6191591802794942 1.8855419471803134E-4 60-61 0.0 1.414156460385235E-4 0.0 2.9206573376336262 1.8855419471803134E-4 62-63 0.0 1.8855419471803134E-4 0.0 3.2376876467776556 1.8855419471803134E-4 64-65 0.0 1.8855419471803134E-4 0.0 3.552313889939031 2.3569274339753918E-4 66-67 0.0 1.8855419471803134E-4 0.0 3.8963074489277396 2.3569274339753918E-4 68-69 0.0 1.8855419471803134E-4 0.0 4.270281124876615 2.3569274339753918E-4 70-71 0.0 1.8855419471803134E-4 0.0 4.667423397501468 2.3569274339753918E-4 72-73 0.0 1.8855419471803134E-4 0.0 5.053676665381356 2.3569274339753918E-4 74-75 0.0 1.8855419471803134E-4 0.0 5.445869390394861 2.3569274339753918E-4 76-77 0.0 1.8855419471803134E-4 0.0 5.862197052332274 2.3569274339753918E-4 78-79 0.0 1.8855419471803134E-4 0.0 6.310838189389489 2.3569274339753918E-4 80-81 0.0 1.8855419471803134E-4 0.0 6.769048451828645 2.3569274339753918E-4 82-83 0.0 1.8855419471803134E-4 0.0 7.236804270375402 2.592620177372931E-4 84-85 0.0 1.8855419471803134E-4 0.0 7.724051878801134 2.82831292077047E-4 86-87 0.0 1.8855419471803134E-4 0.0 8.23776778231041 2.82831292077047E-4 88 0.0 1.8855419471803134E-4 0.0 8.64393708700739 2.82831292077047E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2505 0.0 57.784847 2 ACCTGGG 2750 0.0 51.103474 3 GGTATCA 4865 0.0 49.773727 1 GTACCTG 3095 0.0 46.943302 1 TAACGCA 245 0.0 42.20545 4 CCTGGGG 1975 0.0 40.694984 4 GTATCAA 8530 0.0 39.026535 1 TATCACG 150 0.0 37.59767 2 GTACCCG 130 2.2919266E-10 36.168663 1 CTGGGGG 1460 0.0 34.755955 5 ATAACGC 315 0.0 32.82646 3 ATCAACG 10275 0.0 31.974758 3 CAACGCA 10475 0.0 31.577442 5 TATCAAC 10640 0.0 31.139988 2 TCAACGC 10980 0.0 30.007347 4 AACGCAG 11350 0.0 29.681211 6 GTACATG 9095 0.0 28.899137 1 GTGGTAT 2260 0.0 28.086725 1 TACATGG 9160 0.0 27.859627 2 ACGCAGA 12340 0.0 27.185755 7 >>END_MODULE