FastQCFastQC Report
Mon 6 Jun 2016
ERR1211267_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211267_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5218476
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164540.31530278188497946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130520.250111335186748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80970.15516024218564958No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92200.045.5757641
GTATCAA163150.040.0616231
CAACGCA191250.032.6174165
ATCAACG193800.032.212193
TATCAAC205000.031.5615312
GTACTAG31100.031.471421
AACGCAG212000.030.2010046
TCAACGC215700.029.2903844
TACCTGG32850.027.5026862
ACGCAGA231350.027.1060647
GTGGTAT40050.026.7883341
CGCAGAG235500.026.5874548
GCATCGT32750.026.5536298
CTGTGCG43400.025.1279779
GTACATG200500.024.3610691
GCAGAGT251050.024.2288789
TAGTACT45450.023.8920844
CTAGTAC47450.023.6775993
TAGGCAT46250.023.37745
TACATGG204000.023.2970222
Produced by FastQC (version 0.11.5)