FastQCFastQC Report
Mon 6 Jun 2016
ERR1211267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5218476
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146440.28061832611666704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127120.24359602305347386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78320.15008213125824474No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96400.045.4131
GTATCAA164250.037.789931
CAACGCA193750.031.0654565
GTACTAG31500.030.7513521
ATCAACG196150.030.6231753
TATCAAC209700.029.3591212
AACGCAG212700.028.8279196
TCAACGC215050.028.1066554
GTGGTAT40200.028.073251
TACCTGG32300.026.9177912
ACGCAGA233800.025.6434527
CTGTGCG42950.025.4893579
CGCAGAG238350.025.094558
CTAACGC15350.024.8018633
TAGTACT47100.024.6481154
GTACATG200350.024.4794961
TCTAACG16000.024.3796962
TAGGCAT44300.024.1825125
CTAGGCA47300.024.0470544
TAACGCC16050.023.7201614
Produced by FastQC (version 0.11.5)