##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211267_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5218476 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1393441303553 33.0 31.0 34.0 30.0 34.0 2 32.28259189081257 34.0 31.0 34.0 30.0 34.0 3 32.35996927072195 34.0 31.0 34.0 30.0 34.0 4 35.975900243672676 37.0 35.0 37.0 35.0 37.0 5 35.8594725739852 37.0 35.0 37.0 35.0 37.0 6 35.88803282797507 37.0 35.0 37.0 35.0 37.0 7 35.82820501617714 37.0 35.0 37.0 35.0 37.0 8 35.815913879837716 37.0 35.0 37.0 35.0 37.0 9 37.421064885610285 39.0 37.0 39.0 34.0 39.0 10-11 37.40105387090024 39.0 37.0 39.0 34.0 39.0 12-13 37.303693741237865 39.0 37.0 39.0 34.0 39.0 14-15 38.5251103962153 40.0 38.0 41.0 34.0 41.0 16-17 38.478369930224844 40.0 38.0 41.0 34.0 41.0 18-19 38.5017445706371 40.0 38.0 41.0 34.0 41.0 20-21 38.464837243670374 40.0 38.0 41.0 34.0 41.0 22-23 38.3400524405976 40.0 38.0 41.0 34.0 41.0 24-25 38.27425986054166 40.0 38.0 41.0 34.0 41.0 26-27 38.132151513200405 40.0 38.0 41.0 33.0 41.0 28-29 38.11023199110238 40.0 37.5 41.0 33.5 41.0 30-31 38.1261357722063 40.0 37.5 41.0 33.5 41.0 32-33 37.997046072454864 40.0 37.0 41.0 33.0 41.0 34-35 37.856164903316596 40.0 37.0 41.0 33.0 41.0 36-37 37.71159300148166 40.0 37.0 41.0 33.0 41.0 38-39 37.62154056088406 40.0 37.0 41.0 33.0 41.0 40-41 37.52149296078012 40.0 36.5 41.0 32.0 41.0 42-43 37.38155469144631 40.0 36.0 41.0 32.0 41.0 44-45 37.240826057262694 40.0 36.0 41.0 31.5 41.0 46-47 37.00515571979252 39.0 36.0 41.0 31.0 41.0 48-49 36.77305079107387 39.0 35.0 41.0 30.5 41.0 50-51 36.57542546904499 39.0 35.0 41.0 30.0 41.0 52-53 36.37240767227827 39.0 35.0 40.0 30.0 41.0 54-55 36.13639001118334 38.5 35.0 40.0 29.0 41.0 56-57 35.880007400628074 38.0 35.0 40.0 29.0 41.0 58-59 35.60722967778332 38.0 34.0 40.0 28.0 41.0 60-61 35.34533233457431 37.0 34.0 40.0 28.0 41.0 62-63 35.040224483163286 37.0 34.0 40.0 27.5 41.0 64-65 34.66989624940308 36.0 34.0 39.0 27.0 41.0 66-67 34.36623125218934 36.0 33.0 39.0 26.0 40.5 68-69 34.03597151735488 35.0 33.0 39.0 26.0 40.0 70-71 33.716539943845675 35.0 33.0 38.0 26.0 40.0 72-73 33.37834657091457 35.0 32.5 37.5 26.0 40.0 74-75 33.036925339888505 35.0 32.0 37.0 25.5 39.0 76-77 32.04057372305631 34.0 30.5 36.0 24.5 38.5 78-79 32.54124365044507 35.0 32.0 36.0 25.0 38.5 80-81 32.466092111949926 35.0 32.0 36.0 25.0 37.5 82-83 32.21216893591156 35.0 32.0 35.0 25.0 37.0 84-85 31.956701726711017 34.0 31.5 35.0 25.0 36.5 86-87 31.71556542561468 34.0 31.0 35.0 24.0 36.0 88-89 31.500030564478976 34.0 31.0 35.0 24.0 36.0 90-91 31.28474970470306 34.0 31.0 35.0 23.5 35.5 92-93 31.101889133915726 34.0 31.0 35.0 23.0 35.0 94-95 30.912733621846684 34.0 30.5 35.0 22.0 35.0 96-97 30.740220899741608 34.0 30.0 35.0 20.0 35.0 98-99 30.563155507469997 34.0 30.0 35.0 20.0 35.0 100 28.765848880017845 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3435178818986486 1101 2 -0.31803019665487753 1101 3 -0.3651284233615648 1101 4 -0.2816991423228785 1101 5 -0.303042587943537 1101 6 -0.3712938194130402 1101 7 -0.2909279862673486 1101 8 -0.3479841727555595 1101 9 -0.531907470143473 1101 10-11 -0.46360383075005984 1101 12-13 -0.4266835755610998 1101 14-15 -0.683306162014027 1101 16-17 -0.702645642565642 1101 18-19 -0.7525735808872938 1101 20-21 -0.7755170793482051 1101 22-23 -0.7528575740925234 1101 24-25 -0.6747685663953433 1101 26-27 -0.8209246668252064 1101 28-29 -0.9349948694594516 1101 30-31 -0.8091960209338112 1101 32-33 -0.7864358567816154 1101 34-35 -0.685419248107614 1101 36-37 -0.757372693506575 1101 38-39 -0.7638160321213334 1101 40-41 -0.9050962356342254 1101 42-43 -0.8592097892218575 1101 44-45 -0.8804185532319835 1101 46-47 -1.0566088353922751 1101 48-49 -0.8756817969578989 1101 50-51 -0.9368455029381124 1101 52-53 -0.8496465243129308 1101 54-55 -0.9380207365648232 1101 56-57 -0.9667411370691141 1101 58-59 -0.8954998301278678 1101 60-61 -1.0109342177289804 1101 62-63 -1.1244285190396184 1101 64-65 -0.96292020703752 1101 66-67 -1.0605256823970848 1101 68-69 -0.9913315418718724 1101 70-71 -1.1249667973041966 1101 72-73 -1.1949677697328411 1101 74-75 -1.1829867111148786 1101 76-77 -1.163862036477699 1101 78-79 -0.9959664432211497 1101 80-81 -0.9424460042116429 1101 82-83 -0.9648684163610497 1101 84-85 -0.8771227221607241 1101 86-87 -1.0257981589888097 1101 88-89 -1.1249204861985937 1101 90-91 -1.1545389778851423 1101 92-93 -1.1617376204806789 1101 94-95 -1.1170522330767447 1101 96-97 -1.2456396292824756 1101 98-99 -1.4006537789720177 1101 100 -1.2990113383084356 1102 1 -0.433883577009162 1102 2 -0.38966137948408175 1102 3 -0.42185603124822535 1102 4 -0.2624888752330321 1102 5 -0.33608525285099944 1102 6 -0.43045224523859105 1102 7 -0.44059055639279165 1102 8 -0.4443236373600712 1102 9 -0.5157581392396864 1102 10-11 -0.5264176592672882 1102 12-13 -0.5809529385189549 1102 14-15 -0.8028295574897157 1102 16-17 -0.7878961252022876 1102 18-19 -0.7191092839558877 1102 20-21 -0.7430095728883614 1102 22-23 -0.8305680957147743 1102 24-25 -0.8600846702201039 1102 26-27 -0.8745813741985842 1102 28-29 -0.798544495441142 1102 30-31 -0.8044128332246245 1102 32-33 -0.7263341652068362 1102 34-35 -0.8015017726179536 1102 36-37 -0.8868093281274554 1102 38-39 -0.9433499587200345 1102 40-41 -0.8898011128115826 1102 42-43 -0.8160318025378288 1102 44-45 -0.7982806444580035 1102 46-47 -0.9152418424819118 1102 48-49 -0.9711259775136156 1102 50-51 -0.963061791253466 1102 52-53 -0.9371902120334923 1102 54-55 -0.9742424519736943 1102 56-57 -0.941196592559784 1102 58-59 -0.9832087058439924 1102 60-61 -1.0106094093355793 1102 62-63 -0.9607687215300373 1102 64-65 -0.9185016018751071 1102 66-67 -1.0593057333265037 1102 68-69 -1.0811169881619023 1102 70-71 -1.1036847147989945 1102 72-73 -1.1948863824461569 1102 74-75 -1.3088455070877032 1102 76-77 -0.9649312446324458 1102 78-79 -0.8555144001660793 1102 80-81 -0.8497556100524868 1102 82-83 -0.849004055657943 1102 84-85 -0.892552279767532 1102 86-87 -0.8507599839068334 1102 88-89 -0.945786255766766 1102 90-91 -1.1349494689788067 1102 92-93 -0.986304767238348 1102 94-95 -1.016205356064166 1102 96-97 -1.3107364118131635 1102 98-99 -1.2005551297429058 1102 100 -1.1066768355087717 1103 1 -0.3878105960172391 1103 2 -0.3848822337515685 1103 3 -0.39581990512203546 1103 4 -0.2692405297430369 1103 5 -0.26065484564395547 1103 6 -0.36773940600630084 1103 7 -0.4517128152800822 1103 8 -0.38856443225801485 1103 9 -0.5554810422623433 1103 10-11 -0.5037799044009006 1103 12-13 -0.5471769790057905 1103 14-15 -0.7335236049193625 1103 16-17 -0.6895339746435312 1103 18-19 -0.6884406397706684 1103 20-21 -0.6566658512287376 1103 22-23 -0.7229214808712214 1103 24-25 -0.8545788927231968 1103 26-27 -0.8553516130330721 1103 28-29 -0.8827328819428928 1103 30-31 -0.8431866886306736 1103 32-33 -0.771856101073368 1103 34-35 -0.870345023236176 1103 36-37 -0.7982012169778159 1103 38-39 -0.8091514549088146 1103 40-41 -0.7735647739286335 1103 42-43 -0.8352123517904317 1103 44-45 -0.8509950342779362 1103 46-47 -0.9131261884381772 1103 48-49 -0.9480804144303576 1103 50-51 -1.1625776999894697 1103 52-53 -1.0369571473464987 1103 54-55 -1.002928140159682 1103 56-57 -0.9882099151502004 1103 58-59 -0.9420202592248899 1103 60-61 -0.9454872941434118 1103 62-63 -0.921643784488289 1103 64-65 -0.9474230856885129 1103 66-67 -1.1185289255722495 1103 68-69 -1.0509966398332082 1103 70-71 -1.1615902741139195 1103 72-73 -1.0203955103853986 1103 74-75 -0.9927496008590282 1103 76-77 -0.9110896545726312 1103 78-79 -0.8335639625191291 1103 80-81 -0.7896207602468799 1103 82-83 -0.7937034286047009 1103 84-85 -0.8229424413438977 1103 86-87 -0.8147419956238515 1103 88-89 -0.8908684185835973 1103 90-91 -0.9316730186294997 1103 92-93 -0.9421369379364215 1103 94-95 -1.011013545073105 1103 96-97 -1.0051469282431604 1103 98-99 -0.9919422568595699 1103 100 -1.2669907360321595 1104 1 -0.3297476565502677 1104 2 -0.34340977375782344 1104 3 -0.37302347493202603 1104 4 -0.31403116770022166 1104 5 -0.34003788895530107 1104 6 -0.3723357141873791 1104 7 -0.32515995978771883 1104 8 -0.3577421759316479 1104 9 -0.5223480023705278 1104 10-11 -0.4993099593596284 1104 12-13 -0.5754431572719696 1104 14-15 -0.7019576001830856 1104 16-17 -0.6724037788912298 1104 18-19 -0.7599851333651984 1104 20-21 -0.7506753310858656 1104 22-23 -0.6694006613869945 1104 24-25 -0.72022539873565 1104 26-27 -0.7657619022708388 1104 28-29 -0.9260188342280955 1104 30-31 -0.8286756727193634 1104 32-33 -0.6846709602661178 1104 34-35 -0.7478414418453596 1104 36-37 -0.7516632432927182 1104 38-39 -0.721129303630498 1104 40-41 -0.7902223674346303 1104 42-43 -0.712703025584311 1104 44-45 -0.7944469934879237 1104 46-47 -0.810320484474893 1104 48-49 -0.7688755515809831 1104 50-51 -0.6926402044918518 1104 52-53 -0.7800978606701818 1104 54-55 -0.7885862771434375 1104 56-57 -0.8248755605163609 1104 58-59 -0.8858178648223429 1104 60-61 -0.9058689732890954 1104 62-63 -0.9793632888657484 1104 64-65 -0.8706091146624431 1104 66-67 -0.9590344556408823 1104 68-69 -0.9045767540182439 1104 70-71 -0.9569294279482179 1104 72-73 -0.9062952487683305 1104 74-75 -0.894912364242856 1104 76-77 -0.8175464850212428 1104 78-79 -0.6306447528598156 1104 80-81 -0.7111915386511143 1104 82-83 -0.7133807204440501 1104 84-85 -0.6345244905286371 1104 86-87 -0.7244905596479931 1104 88-89 -0.8541276118838681 1104 90-91 -0.8632696851776238 1104 92-93 -0.9079830306869212 1104 94-95 -0.9375402069433072 1104 96-97 -0.9491324257760816 1104 98-99 -1.0500092997660424 1104 100 -1.2206752599556658 1105 1 -0.44947014229217075 1105 2 -0.39442965099129523 1105 3 -0.5124724833130756 1105 4 -0.2724401367888021 1105 5 -0.3679884683621921 1105 6 -0.38864365767978626 1105 7 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-0.9173750639039397 1105 70-71 -0.9608551983788445 1105 72-73 -0.958334442555099 1105 74-75 -0.9600173123575146 1105 76-77 -0.9818939542230858 1105 78-79 -0.902435475621413 1105 80-81 -0.6910246578046255 1105 82-83 -0.7139674161763807 1105 84-85 -0.6842467295712602 1105 86-87 -0.7870861275833683 1105 88-89 -0.8591516426360535 1105 90-91 -0.8521808891373688 1105 92-93 -0.9311325057781445 1105 94-95 -0.8205272642022088 1105 96-97 -0.8644304721420859 1105 98-99 -0.8809589574147303 1105 100 -1.0257202601462971 1106 1 -0.4620146969792849 1106 2 -0.40418761493850397 1106 3 -0.4823156872121359 1106 4 -0.3205486262061683 1106 5 -0.3849698162730988 1106 6 -0.3795719637393944 1106 7 -0.46842034381144515 1106 8 -0.4779269166969726 1106 9 -0.7181448846565246 1106 10-11 -0.6287490730140561 1106 12-13 -0.6495318979509577 1106 14-15 -0.7844820294108317 1106 16-17 -0.7577331344794302 1106 18-19 -0.7640462915338517 1106 20-21 -0.8423329032583737 1106 22-23 -0.7343363614022351 1106 24-25 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0.5835209863413482 2311 30-31 0.8329825943808018 2311 32-33 0.8966332999185198 2311 34-35 0.9583185548496616 2311 36-37 0.9591072553356099 2311 38-39 0.9907544002132838 2311 40-41 1.0287554710294415 2311 42-43 0.968957232339541 2311 44-45 0.9357931197931535 2311 46-47 0.6304969926066022 2311 48-49 0.8743340479642967 2311 50-51 0.9639823114748012 2311 52-53 0.9095049824682278 2311 54-55 0.9516777936885745 2311 56-57 1.05588943218455 2311 58-59 1.0556143280432337 2311 60-61 1.0774480488232925 2311 62-63 1.0966351056542507 2311 64-65 1.172915473078568 2311 66-67 1.1318189025652785 2311 68-69 1.0881971970331321 2311 70-71 1.1457070423035702 2311 72-73 1.1499962883078183 2311 74-75 1.0871479281553675 2311 76-77 1.074934184579309 2311 78-79 0.8355308498985607 2311 80-81 0.7444174201267302 2311 82-83 0.8526342630862445 2311 84-85 0.9479635262441484 2311 86-87 0.965617913532693 2311 88-89 1.0075272184634123 2311 90-91 1.0313628725179775 2311 92-93 1.076736026339315 2311 94-95 1.1500236267349493 2311 96-97 1.2058772737021393 2311 98-99 1.3007458242900505 2311 100 1.4209734127276334 2312 1 0.16743075769100102 2312 2 0.30157588429581494 2312 3 0.4053773377812391 2312 4 0.15022718054827067 2312 5 0.3202058214543442 2312 6 0.40743741823906277 2312 7 0.30402950372545234 2312 8 0.3873119998934911 2312 9 0.5816962247170423 2312 10-11 0.5890400909170879 2312 12-13 0.6520099869830247 2312 14-15 0.8491524430722208 2312 16-17 0.810710777929863 2312 18-19 0.7526437794200902 2312 20-21 0.7772073662036192 2312 22-23 0.8678895598573675 2312 24-25 0.9031743398718248 2312 26-27 0.9584618353066219 2312 28-29 0.9433032576907223 2312 30-31 0.9444492537316265 2312 32-33 0.801671538948618 2312 34-35 0.9288346944523269 2312 36-37 0.9973489632991672 2312 38-39 0.7628721627962349 2312 40-41 0.9249348279046004 2312 42-43 0.9678030880687558 2312 44-45 0.7700197473572175 2312 46-47 0.7972603810490284 2312 48-49 0.9521555471301397 2312 50-51 0.7709182874885983 2312 52-53 0.9754238556419565 2312 54-55 0.8368519767730334 2312 56-57 0.8995004447279129 2312 58-59 0.9765874500281484 2312 60-61 1.0520520793095898 2312 62-63 1.0571124512538645 2312 64-65 1.0655085187619449 2312 66-67 1.0514107813025717 2312 68-69 1.135286392581385 2312 70-71 1.0780780818638291 2312 72-73 1.0773680216525747 2312 74-75 1.0954065050014137 2312 76-77 1.0311853469211094 2312 78-79 1.0257966051599112 2312 80-81 0.9704825378277135 2312 82-83 0.9778035762898583 2312 84-85 0.9673228464479102 2312 86-87 0.9509666138876831 2312 88-89 1.0200395632643087 2312 90-91 1.1076352976197192 2312 92-93 1.1516285197986456 2312 94-95 1.179144022201406 2312 96-97 0.8788288428437632 2312 98-99 0.5741690985823844 2312 100 0.7684527179200771 2313 1 0.07288751127093462 2313 2 0.252931913256063 2313 3 0.2717883216193613 2313 4 0.17996326272373864 2313 5 0.32141158492190414 2313 6 0.2121594569044163 2313 7 0.2634942290737783 2313 8 0.37017185089370486 2313 9 0.5974356805863863 2313 10-11 0.348758930182008 2313 12-13 0.43892226152873803 2313 14-15 0.40257563896265225 2313 16-17 0.7073259478366012 2313 18-19 0.696019504929339 2313 20-21 0.6086259583032856 2313 22-23 0.6099122784192375 2313 24-25 0.4826475619928061 2313 26-27 0.7177946566764604 2313 28-29 0.5744649878742436 2313 30-31 0.7735732268732889 2313 32-33 0.8399185795458237 2313 34-35 0.9117765003437626 2313 36-37 0.9650042350413202 2313 38-39 0.953378975462094 2313 40-41 1.0500935950208898 2313 42-43 1.081515886402066 2313 44-45 0.9111699839277279 2313 46-47 0.9789423523862908 2313 48-49 1.0343538251046098 2313 50-51 0.9288954325756151 2313 52-53 0.9480383750489594 2313 54-55 0.889732822961804 2313 56-57 0.6007473600131945 2313 58-59 0.7420582614088431 2313 60-61 0.6200990511176556 2313 62-63 0.9102491288589434 2313 64-65 1.0474095591550991 2313 66-67 0.9931656328393998 2313 68-69 1.0550136940067674 2313 70-71 1.0312128636638747 2313 72-73 1.1322092429988828 2313 74-75 1.1602746601521048 2313 76-77 1.097237514913136 2313 78-79 0.9393750740380185 2313 80-81 0.8236363904300035 2313 82-83 0.911288260243559 2313 84-85 0.8951764519111656 2313 86-87 0.9829040718302444 2313 88-89 1.015002883894521 2313 90-91 0.8762185987029518 2313 92-93 0.9594568730339503 2313 94-95 1.1243235419585922 2313 96-97 1.2252806575500337 2313 98-99 1.244314650521634 2313 100 1.3124929375156569 2314 1 -0.33487526952590585 2314 2 0.06741950181039158 2314 3 0.2773184839229401 2314 4 0.27054612881613593 2314 5 0.2953267153735055 2314 6 0.39552128752370663 2314 7 0.48072666060169666 2314 8 0.4791583135432518 2314 9 0.5075507932043664 2314 10-11 0.5846341780246291 2314 12-13 0.4556035313408202 2314 14-15 0.803810440333045 2314 16-17 0.8452569414269462 2314 18-19 0.9012722394299217 2314 20-21 0.9326431905026809 2314 22-23 0.9179112234401785 2314 24-25 0.9019359613055684 2314 26-27 0.8268270532537088 2314 28-29 0.861210262911861 2314 30-31 0.8998930041175797 2314 32-33 0.9319862557513261 2314 34-35 0.9695776683296202 2314 36-37 0.9436330998558873 2314 38-39 0.6762110946645308 2314 40-41 0.6555166269406953 2314 42-43 0.8639723253698364 2314 44-45 0.9093152927856423 2314 46-47 0.8671368131961614 2314 48-49 0.9352626987017558 2314 50-51 0.9635601946348373 2314 52-53 0.9098844511510649 2314 54-55 0.8830422896604517 2314 56-57 0.9677299594188398 2314 58-59 1.0016612277641173 2314 60-61 1.0743021556304768 2314 62-63 0.9857520795050903 2314 64-65 1.0366833499397217 2314 66-67 0.7720049989294964 2314 68-69 0.7630094951840292 2314 70-71 0.8629525214148615 2314 72-73 0.9628574369515306 2314 74-75 0.9885420233155529 2314 76-77 0.8699708225540839 2314 78-79 0.8290235877745715 2314 80-81 0.8963057816602529 2314 82-83 0.9305344876437758 2314 84-85 0.9487559879010341 2314 86-87 1.0355762203577363 2314 88-89 0.9999268540977404 2314 90-91 1.05347483765566 2314 92-93 0.9603059000597547 2314 94-95 0.9016250361119269 2314 96-97 0.9298236933131001 2314 98-99 1.0920010010979517 2314 100 1.0570794582380003 2315 1 0.41599543142082496 2315 2 0.4498818654851675 2315 3 0.4664643873763765 2315 4 0.32993223774852254 2315 5 0.41242650071257003 2315 6 0.4442032112495582 2315 7 0.4255467873958594 2315 8 0.31624087345926455 2315 9 0.5784258253875834 2315 10-11 0.5500468860436527 2315 12-13 0.455698152735323 2315 14-15 0.6916784399297811 2315 16-17 0.8265862717503083 2315 18-19 0.7976068509358285 2315 20-21 0.5859052176706427 2315 22-23 0.8650113700473625 2315 24-25 0.8761181791713355 2315 26-27 0.8221884651810782 2315 28-29 0.8213604738158082 2315 30-31 0.8293273886216994 2315 32-33 0.8238473627902891 2315 34-35 0.9076820646640371 2315 36-37 0.8720687720867772 2315 38-39 0.9067410321969973 2315 40-41 0.8995411975516419 2315 42-43 0.9670638150773314 2315 44-45 1.01192106099343 2315 46-47 1.0141164077179852 2315 48-49 0.9267150253928378 2315 50-51 0.9617460663842579 2315 52-53 1.0206304210459862 2315 54-55 0.893070092377009 2315 56-57 0.8840780532916241 2315 58-59 0.8566560182380627 2315 60-61 1.0239534224226006 2315 62-63 1.0808513083121127 2315 64-65 1.1426016133766268 2315 66-67 1.1324936772544802 2315 68-69 1.1604517716548557 2315 70-71 0.9705872562594848 2315 72-73 1.1180907390247228 2315 74-75 0.5385079665801342 2315 76-77 0.571292257726018 2315 78-79 0.7592171329384172 2315 80-81 0.8042119204216434 2315 82-83 0.8107920956965344 2315 84-85 0.9075425111870494 2315 86-87 0.9751179263732617 2315 88-89 0.9772383953003789 2315 90-91 1.020566322785104 2315 92-93 1.0653573190621977 2315 94-95 1.1496817271408055 2315 96-97 1.2511172939211441 2315 98-99 1.211008242284425 2315 100 1.1701590161453481 2316 1 0.225657725179083 2316 2 0.08736935308149185 2316 3 0.2269349769760538 2316 4 0.03992904124162777 2316 5 0.3002587544093913 2316 6 0.3582819757887705 2316 7 0.4010421191186211 2316 8 0.4486472197939051 2316 9 0.6221277282877082 2316 10-11 0.6363630821859587 2316 12-13 0.3888517640995204 2316 14-15 0.5181862162844766 2316 16-17 0.754414763571674 2316 18-19 0.7395035247565787 2316 20-21 0.7492628473556806 2316 22-23 0.8082932693378169 2316 24-25 0.4532078502500525 2316 26-27 0.7305122851010424 2316 28-29 0.962243579515814 2316 30-31 0.5988747168565709 2316 32-33 0.6538258497915308 2316 34-35 0.7862252403392702 2316 36-37 0.8581255872786642 2316 38-39 0.7921155272612523 2316 40-41 0.7648408682606913 2316 42-43 0.7518804798231926 2316 44-45 0.5493396895977511 2316 46-47 0.7235412104369701 2316 48-49 0.8493833330780092 2316 50-51 0.8635410753395547 2316 52-53 0.8370389752173608 2316 54-55 0.7737035733585174 2316 56-57 0.9112242249853537 2316 58-59 0.8029766648457439 2316 60-61 0.8986139657128049 2316 62-63 0.9268278774092096 2316 64-65 0.9182417301904735 2316 66-67 0.9166276232153905 2316 68-69 0.9382122742820087 2316 70-71 0.8725251657439799 2316 72-73 0.8939039818410919 2316 74-75 0.9355874392797219 2316 76-77 0.8111900274919925 2316 78-79 0.5471778267761067 2316 80-81 0.5712236068798333 2316 82-83 0.5206051181641413 2316 84-85 0.5887259218236629 2316 86-87 0.6518966084991398 2316 88-89 0.6323264587028383 2316 90-91 0.5508431504295892 2316 92-93 0.3187887076001452 2316 94-95 0.40029033190540986 2316 96-97 0.6432525097868336 2316 98-99 0.7908850354269426 2316 100 0.5436534505267154 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 19.0 13 124.0 14 436.0 15 1289.0 16 3100.0 17 6323.0 18 11028.0 19 16054.0 20 22948.0 21 32030.0 22 39290.0 23 41059.0 24 41152.0 25 44630.0 26 52096.0 27 64189.0 28 79065.0 29 97525.0 30 120781.0 31 150503.0 32 191996.0 33 252375.0 34 359141.0 35 502545.0 36 780581.0 37 1167152.0 38 988223.0 39 152807.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.17887601573101 20.45295861311907 13.876209358777682 23.49195601237224 2 15.471566794596736 20.289007748622396 40.537984652990644 23.701440803790224 3 18.84590443646766 25.618015681206547 29.699590455144374 25.836489427181426 4 12.579621253190792 16.68011100009738 38.592470304253446 32.14779744245838 5 13.208243172911018 37.75192220870615 34.67280485720352 14.367029761179317 6 30.740258266972965 37.313729142377966 17.77994188341577 14.166070707233299 7 27.571692033162453 32.188461974784765 22.1481663457529 18.091679646299884 8 26.215853057482686 35.601217673512345 19.662867089932003 18.520062179072973 9 26.144407630968498 15.993017108811674 20.936733886608703 36.92584137361112 10-11 25.471576628471432 26.30239772254301 27.407284765673808 20.818740883311758 12-13 25.65252054934434 23.806665375740483 28.047137521293337 22.49367655362184 14-15 22.927400971012204 25.307264271993795 25.85203101597556 25.913303741018446 16-17 21.964165108816076 28.086707164554152 26.887388169865773 23.061739556764 18-19 21.571796056933096 27.523744480189233 29.149372345489372 21.755087117388292 20-21 22.679269326766917 26.75363971275133 28.837284838961573 21.729806121520177 22-23 22.70276801119714 26.546332684101642 28.598684750107118 22.1522145545941 24-25 22.285409378420557 26.50722015434474 29.13549200835191 22.071878458882793 26-27 22.253982382708283 26.77920322164473 28.54361518093726 22.423199214709726 28-29 21.84964384672556 27.132342155108883 28.80139473843816 22.216619259727395 30-31 22.575042412839768 26.73595614245042 28.75704557131001 21.931955873399804 32-33 22.624085331583384 26.904799163687926 28.292985977743513 22.17812952698518 34-35 22.605019772520652 26.8948833343652 28.722751327416496 21.777345565697654 36-37 22.46711716482319 26.65700879894687 28.501389975785045 22.374484060444896 38-39 22.497164717411884 26.712118717503504 28.828474843180167 21.962241721904444 40-41 22.371757172359885 26.264453421651574 29.104432564687922 22.25935684130062 42-43 22.3188383579494 26.407954431131703 28.644489050920047 22.62871815999885 44-45 22.26744262371427 26.949459845008544 28.543903817253703 22.239193714023486 46-47 22.30792782993893 26.74443643193748 28.09976928771475 22.847866450408837 48-49 22.75430173702197 26.740715700652647 28.088405819597533 22.41657674272785 50-51 22.571271063517482 26.79969455018932 27.942841137446955 22.68619324884625 52-53 22.25807842603648 27.17707856210628 27.70037237131342 22.864470640543818 54-55 22.222343598090514 27.073941476261464 27.767288347475862 22.93642657817216 56-57 22.815977142660042 26.72662694226548 27.51489665044539 22.94249926462909 58-59 22.17418051047952 27.036480207587722 28.01362006962391 22.775719212308847 60-61 22.362097076483316 27.40605254342655 27.538309880963606 22.693540499126524 62-63 22.5436718356112 27.015331684427192 27.339999605243666 23.100996874717946 64-65 22.034720151530095 27.055304708277063 27.525376182259375 23.384598957933473 66-67 22.263136066798697 26.827629394387543 27.51863652304095 23.39059801577281 68-69 22.011902652797176 26.900881146802323 27.663831642595998 23.423384557804503 70-71 22.03101091463486 26.617807176254033 27.741777404640978 23.609404504470135 72-73 22.34447051597181 26.796164376576037 27.685728639333707 23.173636468118445 74-75 22.477120409273525 27.05383408304704 27.314997456225804 23.154048051453632 76-77 22.275509718022047 26.929613766237075 27.218119567454067 23.576756948286807 78-79 22.151340629012783 26.898805987312418 27.309561684267013 23.640291699407783 80-81 21.95093954701606 27.210463552275122 27.468779337190057 23.36981756351876 82-83 22.559037416250817 26.645172229465736 27.978353865926735 22.81743648835671 84-85 22.642287357183488 26.93465770146539 27.23159756059918 23.191457380751938 86-87 21.907681275641362 27.316205459707444 27.523478966115416 23.252634298535778 88-89 22.283868659244167 26.645489106902957 27.432840251323405 23.63780198252947 90-91 22.30949209394778 26.586779841426498 27.492789499394554 23.610938565231162 92-93 22.382027727509737 26.796000368064288 27.374858141888787 23.44711376253719 94-95 21.72420824747326 26.736328316651946 27.71549728314199 23.823966152732805 96-97 21.898176631521316 26.717617667808835 27.497146330645446 23.887059370024406 98-99 21.58597945637263 26.961311501552437 27.388755880211306 24.063953161863626 100 21.84390776148151 26.675166234212348 27.1030583107905 24.37786769351564 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 774.0 1 542.5 2 563.0 3 847.5 4 669.5 5 514.0 6 772.5 7 1429.0 8 2223.0 9 2796.5 10 2718.0 11 2437.0 12 2647.0 13 3065.5 14 3567.5 15 4206.5 16 4928.0 17 5563.5 18 6225.0 19 6998.5 20 7720.0 21 8351.5 22 9410.5 23 11003.0 24 12868.5 25 15290.5 26 18749.5 27 23012.5 28 28603.5 29 37758.0 30 49520.0 31 61060.5 32 75440.5 33 91090.5 34 104726.0 35 118132.0 36 131836.5 37 150173.0 38 160372.0 39 158589.0 40 154771.5 41 151868.5 42 154288.0 43 161396.0 44 171411.0 45 190664.5 46 208195.5 47 218971.0 48 228398.5 49 228212.5 50 221836.5 51 220538.0 52 216696.0 53 205897.0 54 194996.0 55 174124.5 56 152362.5 57 129024.5 58 107009.5 59 95832.5 60 78342.0 61 58428.5 62 45360.5 63 35857.0 64 26130.5 65 16557.0 66 11109.5 67 8213.5 68 6224.0 69 4677.5 70 3620.5 71 2749.5 72 2160.5 73 1759.5 74 1372.5 75 987.0 76 608.5 77 353.5 78 226.0 79 151.5 80 92.5 81 63.0 82 48.5 83 32.5 84 17.5 85 9.5 86 5.5 87 5.0 88 3.5 89 2.5 90 3.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04372924202391656 2 0.0 3 0.0 4 0.036485747946335294 5 0.0 6 0.0 7 8.431580407766559E-4 8 0.0 9 0.011114355992055918 10-11 4.599043858781759E-4 12-13 9.581341372461998E-6 14-15 7.665073097969598E-5 16-17 2.7785889980139796E-4 18-19 0.0 20-21 1.9162682744923996E-5 22-23 0.0 24-25 5.365551168578719E-4 26-27 4.5032304450571387E-4 28-29 1.2455743784200597E-4 30-31 0.0024336607086053474 32-33 0.015454703633781205 34-35 0.026578640967209584 36-37 0.03178130933245645 38-39 0.02266945368724509 40-41 0.0014372012058692998 42-43 1.9162682744923996E-5 44-45 0.01102812391970376 46-47 0.01798417775611117 48-49 0.0033917948458515477 50-51 0.011449702940092088 52-53 0.010846078433626982 54-55 0.010060408441085099 56-57 4.0241633764340396E-4 58-59 0.008450743090511482 60-61 0.004599043858781759 62-63 0.0012838997439099079 64-65 0.034923989302623984 66-67 0.058350368958293576 68-69 0.057488048234771986 70-71 0.05049366903287473 72-73 0.04350887117234994 74-75 0.034837757230271826 76-77 0.03059322300227116 78-79 0.014870241810061021 80-81 0.0010922729164606678 82-83 0.037031884404565625 84-85 0.04991878855052701 86-87 0.05563884934988683 88-89 0.06749096862762231 90-91 0.04737015174545212 92-93 0.0382487147588683 94-95 0.013107274997528014 96-97 0.0037271417938877175 98-99 0.022784429783714633 100 0.033343067976167755 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5218476.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.90567838159035 #Duplication Level Percentage of deduplicated Percentage of total 1 80.11693778430765 56.00628885667693 2 13.056075674905228 18.2538765411126 3 3.5767612531792725 7.5010776543745274 4 1.3377844159617027 3.7407490850448983 5 0.6153418791031973 2.1507945747655777 6 0.35645144532078027 1.49507880571485 7 0.21847352195326422 1.0690777832390745 8 0.14826578213146996 0.8291696064541988 9 0.10326837037356125 0.6497140937692613 >10 0.44402752838152887 5.254104332224245 >50 0.018199454471993476 0.8617897535089635 >100 0.007681156465520938 0.9561316591548072 >500 4.4540326358544707E-4 0.22342449403569176 >1k 1.9088678751906363E-4 0.22613751263168164 >5k 3.181446458643512E-5 0.17418459863578648 >10k+ 6.362892917287025E-5 0.608400648656866 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14644 0.28061832611666704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12712 0.24359602305347386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7832 0.15008213125824474 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.9162682744923996E-5 0.0 7 0.0 0.0 0.0 1.9162682744923996E-5 1.9162682744923996E-5 8 0.0 0.0 0.0 1.9162682744923996E-5 1.9162682744923996E-5 9 0.0 0.0 0.0 3.832536548984799E-5 1.9162682744923996E-5 10-11 7.665073097969598E-5 0.0 0.0 5.7488048234771994E-5 2.8744024117385993E-5 12-13 9.581341372461998E-5 0.0 0.0 8.623207235215798E-5 5.7488048234771994E-5 14-15 9.581341372461998E-5 0.0 0.0 9.581341372461998E-5 5.7488048234771994E-5 16-17 9.581341372461998E-5 0.0 0.0 1.0539475509708198E-4 7.665073097969598E-5 18-19 9.581341372461998E-5 0.0 0.0 1.3413877921446797E-4 7.665073097969598E-5 20-21 9.581341372461998E-5 1.9162682744923996E-5 0.0 2.4911487568401194E-4 7.665073097969598E-5 22-23 9.581341372461998E-5 1.9162682744923996E-5 0.0 6.419498719549539E-4 8.623207235215798E-5 24-25 1.4372012058692997E-4 1.9162682744923996E-5 0.0 0.0012551557197925218 9.581341372461998E-5 26-27 1.7246414470431596E-4 1.9162682744923996E-5 0.0 0.002194127174293798 9.581341372461998E-5 28-29 1.7246414470431596E-4 1.9162682744923996E-5 0.0 0.0054422018995584145 9.581341372461998E-5 30-31 1.7246414470431596E-4 1.9162682744923996E-5 0.0 0.018271617997285033 9.581341372461998E-5 32-33 1.7246414470431596E-4 1.9162682744923996E-5 0.0 0.046670713825262396 1.3413877921446797E-4 34-35 1.8204548607677796E-4 1.9162682744923996E-5 0.0 0.08773634294763452 1.3413877921446797E-4 36-37 1.9162682744923996E-4 1.9162682744923996E-5 0.0 0.15563930925427272 1.3413877921446797E-4 38-39 1.9162682744923996E-4 1.9162682744923996E-5 0.0 0.25437503209749357 1.3413877921446797E-4 40-41 1.9162682744923996E-4 4.790670686230999E-5 0.0 0.3576714734340064 1.4372012058692997E-4 42-43 1.9162682744923996E-4 7.665073097969598E-5 0.0 0.45817016308976033 1.5330146195939197E-4 44-45 2.0120816882170198E-4 7.665073097969598E-5 0.0 0.5767775879394674 1.5330146195939197E-4 46-47 2.3953353431154997E-4 7.665073097969598E-5 0.0 0.7151034133337013 1.5330146195939197E-4 48-49 2.4911487568401194E-4 7.665073097969598E-5 0.0 0.8671497195733007 1.5330146195939197E-4 50-51 2.4911487568401194E-4 7.665073097969598E-5 0.0 1.0249831560018672 1.5330146195939197E-4 52-53 2.6827755842893594E-4 7.665073097969598E-5 0.0 1.1749119857981527 1.5330146195939197E-4 54-55 2.8744024117385993E-4 7.665073097969598E-5 0.0 1.3400655670352801 1.5330146195939197E-4 56-57 3.1618426529124596E-4 7.665073097969598E-5 0.0 1.5341739618999877 1.5330146195939197E-4 58-59 3.257656066637079E-4 7.665073097969598E-5 0.0 1.751900363247814 1.5330146195939197E-4 60-61 3.449282894086319E-4 7.665073097969598E-5 0.0 1.9720316812801286 1.5330146195939197E-4 62-63 3.449282894086319E-4 7.665073097969598E-5 0.0 2.198735799493952 1.5330146195939197E-4 64-65 3.449282894086319E-4 7.665073097969598E-5 0.0 2.427116652447956 1.6288280333185396E-4 66-67 3.640909721535559E-4 7.665073097969598E-5 0.0 2.6854679412150215 1.7246414470431596E-4 68-69 4.2157902038832796E-4 7.665073097969598E-5 0.0 2.967782164754614 1.7246414470431596E-4 70-71 4.2157902038832796E-4 7.665073097969598E-5 0.0 3.265177419614462 1.7246414470431596E-4 72-73 4.2157902038832796E-4 7.665073097969598E-5 0.0 3.5624768610605857 1.9162682744923996E-4 74-75 4.2157902038832796E-4 7.665073097969598E-5 0.0 3.8573905485049655 1.9162682744923996E-4 76-77 4.2157902038832796E-4 7.665073097969598E-5 0.0 4.18858877572686 1.9162682744923996E-4 78-79 4.2157902038832796E-4 7.665073097969598E-5 0.0 4.5467776415949785 1.9162682744923996E-4 80-81 4.2157902038832796E-4 7.665073097969598E-5 0.0 4.926332898723689 1.9162682744923996E-4 82-83 4.3116036176078993E-4 7.665073097969598E-5 0.0 5.3063001535314145 1.9162682744923996E-4 84-85 4.4074170313325195E-4 7.665073097969598E-5 0.0 5.681687527163103 1.9162682744923996E-4 86-87 4.4074170313325195E-4 7.665073097969598E-5 0.0 6.0780484570591105 2.0120816882170198E-4 88 4.4074170313325195E-4 7.665073097969598E-5 0.0 6.393092542727033 2.1078951019416398E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9640 0.0 45.413 1 GTATCAA 16425 0.0 37.78993 1 CAACGCA 19375 0.0 31.065456 5 GTACTAG 3150 0.0 30.751352 1 ATCAACG 19615 0.0 30.623175 3 TATCAAC 20970 0.0 29.359121 2 AACGCAG 21270 0.0 28.827919 6 TCAACGC 21505 0.0 28.106655 4 GTGGTAT 4020 0.0 28.07325 1 TACCTGG 3230 0.0 26.917791 2 ACGCAGA 23380 0.0 25.643452 7 CTGTGCG 4295 0.0 25.489357 9 CGCAGAG 23835 0.0 25.09455 8 CTAACGC 1535 0.0 24.801863 3 TAGTACT 4710 0.0 24.648115 4 GTACATG 20035 0.0 24.479496 1 TCTAACG 1600 0.0 24.379696 2 TAGGCAT 4430 0.0 24.182512 5 CTAGGCA 4730 0.0 24.047054 4 TAACGCC 1605 0.0 23.720161 4 >>END_MODULE