FastQCFastQC Report
Mon 6 Jun 2016
ERR1211266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4086551
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158360.3875150463067756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126140.30867105292458114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74350.18193826530000481No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75750.050.7639431
GTATCAA132700.039.937731
TACCTGG30600.035.530922
ATCAACG154350.033.0118063
CAACGCA155450.032.8088465
TATCAAC163750.031.8474252
GTGGTAT29150.031.6470621
AACGCAG170000.030.4715986
TCAACGC168500.030.379454
GTACCTG38200.029.2012121
GTACTAG23900.028.949121
ACCTGGG37050.027.9113663
ACGCAGA185150.027.4961957
CGCAGAG187650.027.1034958
GTACATG149350.026.8188551
GCATCGT22200.026.6786698
TGGTATC34500.026.4666232
TACATGG150250.026.1256732
GCAGAGT202250.024.5897679
ACATGGG152900.024.4095423
Produced by FastQC (version 0.11.5)