##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211266_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4086551 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.418328561175425 31.0 31.0 34.0 28.0 34.0 2 31.424275874692373 31.0 31.0 34.0 28.0 34.0 3 31.487329290641423 31.0 31.0 34.0 28.0 34.0 4 35.08325382455768 37.0 35.0 37.0 32.0 37.0 5 35.14563650374117 37.0 35.0 37.0 32.0 37.0 6 35.207884839807456 37.0 35.0 37.0 32.0 37.0 7 35.129110832092884 37.0 35.0 37.0 32.0 37.0 8 35.13258221908891 37.0 35.0 37.0 32.0 37.0 9 36.69908756797603 39.0 37.0 39.0 32.0 39.0 10-11 36.64502890089956 39.0 37.0 39.0 32.0 39.0 12-13 36.545888207439475 39.0 37.0 39.0 32.0 39.0 14-15 37.73379862382728 40.0 37.0 41.0 32.0 41.0 16-17 37.70960034513212 40.0 37.0 41.0 32.0 41.0 18-19 37.732195805215696 40.0 37.0 41.0 32.0 41.0 20-21 37.70126544364673 40.0 37.0 41.0 32.0 41.0 22-23 37.58677500904797 40.0 37.0 41.0 32.0 41.0 24-25 37.496670541980265 40.0 37.0 41.0 31.5 41.0 26-27 37.352405855206506 40.0 37.0 41.0 31.0 41.0 28-29 37.227061279793155 40.0 36.0 41.0 31.0 41.0 30-31 37.06365074117514 39.5 36.0 41.0 30.5 41.0 32-33 36.86613405779103 39.0 36.0 41.0 30.0 41.0 34-35 36.7010975759265 39.0 35.0 41.0 30.0 41.0 36-37 36.58534923459905 39.0 35.0 41.0 30.0 41.0 38-39 36.44246309418382 39.0 35.0 41.0 29.5 41.0 40-41 36.281920866765155 39.0 35.0 40.5 29.0 41.0 42-43 36.07077080403499 39.0 35.0 40.0 28.0 41.0 44-45 35.858326251158985 38.5 35.0 40.0 27.0 41.0 46-47 35.6186466289054 38.0 34.5 40.0 26.5 41.0 48-49 35.416530590221434 38.0 34.0 40.0 26.0 41.0 50-51 34.73953940621321 37.5 33.5 39.5 24.5 40.5 52-53 34.85105814169455 38.0 33.5 40.0 25.5 40.5 54-55 35.35046509880826 38.0 34.0 40.0 26.0 41.0 56-57 35.324024831697926 38.0 34.0 40.0 26.0 41.0 58-59 35.129311857358445 38.0 34.0 40.0 26.0 41.0 60-61 34.90941578852191 37.5 34.0 40.0 26.0 41.0 62-63 34.6203569954223 37.0 33.5 40.0 25.5 41.0 64-65 34.38135863225493 36.5 33.0 40.0 25.0 41.0 66-67 34.21332928427909 36.0 33.0 39.5 25.0 41.0 68-69 33.91899024385111 36.0 33.0 39.0 24.5 41.0 70-71 33.609554120332774 35.0 33.0 39.0 24.0 41.0 72-73 33.26174138044527 35.0 32.0 38.5 23.5 40.0 74-75 32.926477609113405 35.0 32.0 37.0 23.0 40.0 76-77 32.585921477549164 35.0 32.0 37.0 22.5 39.0 78-79 32.240407130609654 35.0 31.5 36.5 22.0 39.0 80-81 31.934705085046048 35.0 31.0 36.0 21.0 38.5 82-83 31.634246703393643 35.0 31.0 35.5 20.0 37.0 84-85 31.373108643450188 35.0 31.0 35.0 20.0 37.0 86-87 31.138495273887443 34.0 31.0 35.0 20.0 36.5 88-89 30.91521199662013 34.0 30.0 35.0 19.0 36.0 90-91 30.68580680872452 34.0 30.0 35.0 19.0 36.0 92-93 30.493619068989965 34.0 30.0 35.0 18.0 35.5 94-95 30.321340661110064 34.0 29.0 35.0 18.0 35.0 96-97 30.1380686304906 34.0 29.0 35.0 17.5 35.0 98-99 29.91389890888429 34.0 29.0 35.0 17.0 35.0 100 28.80487751162288 33.0 26.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.43062916689428477 1101 2 -0.3654240918530398 1101 3 -0.3664085397298429 1101 4 -0.37216595303510047 1101 5 -0.3006483097025665 1101 6 -0.19806903600873937 1101 7 -0.3229795699519187 1101 8 -0.31217853496050196 1101 9 -0.5037264127112593 1101 10-11 -0.495108693170927 1101 12-13 -0.4764097760959842 1101 14-15 -0.736644199880601 1101 16-17 -0.5997058235651096 1101 18-19 -0.6810136266628888 1101 20-21 -0.7210214557585459 1101 22-23 -0.799885743330286 1101 24-25 -0.6893410035672503 1101 26-27 -0.6824166523453741 1101 28-29 -0.6977106989982502 1101 30-31 -0.8322279106802455 1101 32-33 -0.8631153960665969 1101 34-35 -1.0001815028904701 1101 36-37 -0.9908990150268124 1101 38-39 -0.9979676595469726 1101 40-41 -0.9792720822357026 1101 42-43 -0.9504142167862639 1101 44-45 -0.9793603738441092 1101 46-47 -1.121849865652834 1101 48-49 -1.1302436620475902 1101 50-51 -1.292181623615484 1101 52-53 -1.1120521921383428 1101 54-55 -0.97268157837091 1101 56-57 -0.8965924317060185 1101 58-59 -0.9348901593508216 1101 60-61 -0.8937664473609388 1101 62-63 -0.9549631199318469 1101 64-65 -0.9587616024816654 1101 66-67 -0.8590522696186653 1101 68-69 -0.8674608950148084 1101 70-71 -1.0365532414242082 1101 72-73 -1.1669080450458793 1101 74-75 -1.150864590166961 1101 76-77 -1.2078721819868363 1101 78-79 -1.149736208805173 1101 80-81 -1.1080529172556588 1101 82-83 -1.0899991351593528 1101 84-85 -1.2563181531191638 1101 86-87 -1.2645234998734622 1101 88-89 -1.3326343901650937 1101 90-91 -1.4725726483240145 1101 92-93 -1.4862088760542278 1101 94-95 -1.5072781762114467 1101 96-97 -1.5810272741830396 1101 98-99 -1.568925445595017 1101 100 -1.7576708673851265 1102 1 -0.4084925155997574 1102 2 -0.47449189829775307 1102 3 -0.5558594093723386 1102 4 -0.30763090530279413 1102 5 -0.4394284507929953 1102 6 -0.3373130773902133 1102 7 -0.42310553862137823 1102 8 -0.49379745843459233 1102 9 -0.5676650379479682 1102 10-11 -0.6096764976923978 1102 12-13 -0.5613051260108577 1102 14-15 -0.7677401432714106 1102 16-17 -0.8185223364123999 1102 18-19 -0.6895946571326164 1102 20-21 -0.8178151731899987 1102 22-23 -0.7346949792134936 1102 24-25 -0.7973814726261779 1102 26-27 -0.7363415775730218 1102 28-29 -0.797784921593319 1102 30-31 -0.7099111859498421 1102 32-33 -0.8272220182359931 1102 34-35 -0.8158607158392144 1102 36-37 -0.8921044735772199 1102 38-39 -1.0500614777199786 1102 40-41 -0.8781726632214699 1102 42-43 -0.8944700697426455 1102 44-45 -1.0736322140441033 1102 46-47 -1.0510832987261765 1102 48-49 -1.0208026379485062 1102 50-51 -1.073057501039841 1102 52-53 -0.8637865383883749 1102 54-55 -0.7535867398640264 1102 56-57 -0.8512177650889967 1102 58-59 -0.8377414406249954 1102 60-61 -0.7605363086183203 1102 62-63 -0.8304037508188244 1102 64-65 -0.8441400560694703 1102 66-67 -0.7796387014205663 1102 68-69 -0.9324376713330835 1102 70-71 -1.012172240231287 1102 72-73 -1.0159624962134473 1102 74-75 -0.9889321087060949 1102 76-77 -1.016204141835658 1102 78-79 -0.958128805182966 1102 80-81 -1.0108663868822028 1102 82-83 -1.0041616510723586 1102 84-85 -0.9326215282332804 1102 86-87 -1.1540073476592987 1102 88-89 -1.2534476918527666 1102 90-91 -1.196942828563646 1102 92-93 -1.2604476118301342 1102 94-95 -1.2373880339257077 1102 96-97 -1.418864910968125 1102 98-99 -1.343363000965308 1102 100 -1.4053458538480506 1103 1 -0.529890996059315 1103 2 -0.3930862342345023 1103 3 -0.5007464337955909 1103 4 -0.42143261073824334 1103 5 -0.3293875102430235 1103 6 -0.35055010821109533 1103 7 -0.40809500214626127 1103 8 -0.3718496519656682 1103 9 -0.5419611881440574 1103 10-11 -0.5048047277286187 1103 12-13 -0.5443049410002487 1103 14-15 -0.7440497280616896 1103 16-17 -0.7263473292195215 1103 18-19 -0.5829962760681155 1103 20-21 -0.6226906447922929 1103 22-23 -0.6687623068116437 1103 24-25 -0.6771234748403216 1103 26-27 -0.7958546173970902 1103 28-29 -0.7818301106828613 1103 30-31 -0.9071623021115371 1103 32-33 -0.949202419094199 1103 34-35 -0.913144269480668 1103 36-37 -0.837175688395078 1103 38-39 -0.8290875302655607 1103 40-41 -0.9172994816424804 1103 42-43 -0.939699884436763 1103 44-45 -0.9513865647531716 1103 46-47 -1.1844415307494458 1103 48-49 -1.1578509542450348 1103 50-51 -1.0949571505467404 1103 52-53 -1.0045044337351428 1103 54-55 -0.7881735949248352 1103 56-57 -0.708027974022599 1103 58-59 -0.809117647063168 1103 60-61 -0.8090668936383949 1103 62-63 -0.7824235973070941 1103 64-65 -0.7524317477525031 1103 66-67 -0.9300199416591894 1103 68-69 -0.8924881120420309 1103 70-71 -0.8563091871626298 1103 72-73 -0.7986360891521684 1103 74-75 -0.8750251312387292 1103 76-77 -0.9024998629554943 1103 78-79 -0.9347681496206413 1103 80-81 -0.9242474035960626 1103 82-83 -0.8565498284240647 1103 84-85 -1.0795032936195774 1103 86-87 -1.084058861058871 1103 88-89 -1.0375143561515259 1103 90-91 -1.2022286827841349 1103 92-93 -1.347218904346736 1103 94-95 -1.4399344188798509 1103 96-97 -1.3066949576886522 1103 98-99 -1.4099916384670586 1103 100 -1.4382230369054163 1104 1 -0.5638857821471497 1104 2 -0.4168548778522414 1104 3 -0.5153807454781756 1104 4 -0.3052264463053618 1104 5 -0.3656026251876199 1104 6 -0.4711022777116227 1104 7 -0.5568691308356009 1104 8 -0.4132179213608822 1104 9 -0.512565282989101 1104 10-11 -0.5683186221182055 1104 12-13 -0.4947421152713787 1104 14-15 -0.7591820561173463 1104 16-17 -0.7904444695563413 1104 18-19 -0.7901543507686029 1104 20-21 -0.7756334279007504 1104 22-23 -0.7952886334676279 1104 24-25 -0.7587639134885293 1104 26-27 -0.8049030165437827 1104 28-29 -0.7969285438088676 1104 30-31 -0.858742410597813 1104 32-33 -0.8960166332493955 1104 34-35 -1.0112385165125346 1104 36-37 -0.9177888715818341 1104 38-39 -0.9153091991675097 1104 40-41 -0.827578548021414 1104 42-43 -0.8483882097290731 1104 44-45 -0.8620050023572503 1104 46-47 -0.8859124300536578 1104 48-49 -0.9922167702661682 1104 50-51 -0.9957708820156412 1104 52-53 -0.8772621645919614 1104 54-55 -0.6787226477795443 1104 56-57 -0.5756992151452138 1104 58-59 -0.614152113002838 1104 60-61 -0.8019583111609236 1104 62-63 -0.7894489895319126 1104 64-65 -0.6998271685334458 1104 66-67 -0.7041053130013495 1104 68-69 -0.8886430065261308 1104 70-71 -0.7427444363142754 1104 72-73 -0.7792230143166705 1104 74-75 -0.8055424944438627 1104 76-77 -0.7320739766804358 1104 78-79 -0.7650689650116966 1104 80-81 -0.7103147028930721 1104 82-83 -0.8725314987211945 1104 84-85 -0.7218337289598651 1104 86-87 -0.8106356363093283 1104 88-89 -0.8726068738387731 1104 90-91 -0.9080715357639626 1104 92-93 -1.0312699194506543 1104 94-95 -1.0078129535162503 1104 96-97 -1.0926282243385153 1104 98-99 -0.9107774101350614 1104 100 -0.9934187498859615 1105 1 -0.5581140683186767 1105 2 -0.6103470943358396 1105 3 -0.6053105938087207 1105 4 -0.3987445775339893 1105 5 -0.32980504207857564 1105 6 -0.43553429015069867 1105 7 -0.47039912828959274 1105 8 -0.49594165199302154 1105 9 -0.8032798479851024 1105 10-11 -0.7782360131853423 1105 12-13 -0.6391190702433747 1105 14-15 -0.8404202353564543 1105 16-17 -0.8960584216913574 1105 18-19 -0.7886642529290739 1105 20-21 -0.8340518555576963 1105 22-23 -0.8648725654164195 1105 24-25 -0.9103202757857574 1105 26-27 -0.8605719806814207 1105 28-29 -0.9200156264450783 1105 30-31 -0.9449754296201363 1105 32-33 -1.0606434609208932 1105 34-35 -1.0353989015056229 1105 36-37 -0.9181031446959764 1105 38-39 -0.7984336990583927 1105 40-41 -1.0651502682149 1105 42-43 -1.065025248719401 1105 44-45 -1.0167347843096763 1105 46-47 -1.2141181294789831 1105 48-49 -1.1478058306066075 1105 50-51 -1.1416856725153863 1105 52-53 -1.0704577254415995 1105 54-55 -0.8606225623063537 1105 56-57 -0.8772229566888328 1105 58-59 -0.8309045180730337 1105 60-61 -0.7342009140924688 1105 62-63 -0.8505282250014616 1105 64-65 -0.9510939221887895 1105 66-67 -0.7784919973688531 1105 68-69 -0.8455387155899245 1105 70-71 -0.8118129148914122 1105 72-73 -0.6345948681756894 1105 74-75 -0.8200070139753919 1105 76-77 -0.8716004415454961 1105 78-79 -0.947933294501766 1105 80-81 -0.7587639099928047 1105 82-83 -0.8529238115894557 1105 84-85 -1.0063082961381618 1105 86-87 -1.0477646462751409 1105 88-89 -1.0204340133964926 1105 90-91 -1.0369127147484711 1105 92-93 -0.9540292032944002 1105 94-95 -0.9991684538072221 1105 96-97 -1.0618453717161245 1105 98-99 -1.2154973035663552 1105 100 -1.271325975098737 1106 1 -0.5058449083745522 1106 2 -0.4926998906763451 1106 3 -0.6039507496343397 1106 4 -0.447177682486668 1106 5 -0.3294968435618699 1106 6 -0.3197511212086681 1106 7 -0.38942839230506365 1106 8 -0.34401617454912525 1106 9 -0.5431951225877967 1106 10-11 -0.45966803392082767 1106 12-13 -0.595825690862462 1106 14-15 -0.7183367599183512 1106 16-17 -0.7035161645160528 1106 18-19 -0.7694441604962492 1106 20-21 -0.6588973482757368 1106 22-23 -0.832632756438997 1106 24-25 -0.7711935490017368 1106 26-27 -0.8061965395938344 1106 28-29 -0.8025499932881246 1106 30-31 -0.8424186622813892 1106 32-33 -0.928064528051749 1106 34-35 -0.9304398952147466 1106 36-37 -0.9175738747711932 1106 38-39 -0.9166414779017202 1106 40-41 -0.9424121611179928 1106 42-43 -0.8585232378665353 1106 44-45 -1.0047769491872245 1106 46-47 -0.8462024392550092 1106 48-49 -0.8528042744890101 1106 50-51 -0.7750571912298767 1106 52-53 -0.8267981659832699 1106 54-55 -0.7054702513169531 1106 56-57 -0.7989104944661918 1106 58-59 -0.7345982853858999 1106 60-61 -0.7509086363752786 1106 62-63 -0.7694104949971532 1106 64-65 -0.7914341111628076 1106 66-67 -0.7639165853212901 1106 68-69 -0.7628159755060224 1106 70-71 -0.7933387318109411 1106 72-73 -0.9028512158973427 1106 74-75 -0.8309070479117011 1106 76-77 -0.8391816649747206 1106 78-79 -0.6992356636274941 1106 80-81 -0.7456986425375547 1106 82-83 -0.7443494347962627 1106 84-85 -0.8358937216476825 1106 86-87 -0.8217728253192718 1106 88-89 -0.9250580041712233 1106 90-91 -0.8983168759138529 1106 92-93 -0.9024877675128131 1106 94-95 -1.0775436320713254 1106 96-97 -1.1688153012208993 1106 98-99 -0.9442547507005692 1106 100 -1.110756306616505 1107 1 -0.6183048270717251 1107 2 -0.5804565836073223 1107 3 -0.6162250731054257 1107 4 -0.4871115517335127 1107 5 -0.48129345003336965 1107 6 -0.46012580176086004 1107 7 -0.45870305331349925 1107 8 -0.5227972520883171 1107 9 -0.63388116474394 1107 10-11 -0.5798487422018042 1107 12-13 -0.6477114418146428 1107 14-15 -0.7762055969683175 1107 16-17 -0.8594096130106053 1107 18-19 -0.8795997879604869 1107 20-21 -0.8793217194790728 1107 22-23 -0.8766586371206486 1107 24-25 -0.8223070096573011 1107 26-27 -0.7832515473137178 1107 28-29 -1.0226121710098894 1107 30-31 -0.8954024663253577 1107 32-33 -0.9417421374097259 1107 34-35 -0.9774127366412273 1107 36-37 -1.0008886405309454 1107 38-39 -0.9845094313565852 1107 40-41 -0.9554951049348333 1107 42-43 -0.9279223871596898 1107 44-45 -0.8923770102941688 1107 46-47 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56-57 1.0288021872870345 2311 58-59 0.9624034039319938 2311 60-61 1.0204750710687946 2311 62-63 1.0895506058084194 2311 64-65 1.0864006994024678 2311 66-67 1.0994089145463093 2311 68-69 1.046387601457127 2311 70-71 1.0082782358182882 2311 72-73 0.9480519765516391 2311 74-75 0.9949166420911766 2311 76-77 0.9875322041827417 2311 78-79 1.0388689745633073 2311 80-81 0.9400701938112768 2311 82-83 1.017824921849126 2311 84-85 0.987880755439253 2311 86-87 0.9803057166864022 2311 88-89 1.024251155298515 2311 90-91 1.1336376466533942 2311 92-93 1.1196962838568147 2311 94-95 1.2226128377635135 2311 96-97 1.2930949772536238 2311 98-99 1.307921727635442 2311 100 1.4392503454848011 2312 1 0.7126033970095982 2312 2 0.6924111257897216 2312 3 0.706808549240975 2312 4 0.48590764110183926 2312 5 0.5827685577511659 2312 6 0.6122151511999903 2312 7 0.5040502031047254 2312 8 0.6207149648977008 2312 9 0.8387177223337687 2312 10-11 0.8224727177053026 2312 12-13 0.9170023517407984 2312 14-15 1.1196691485331698 2312 16-17 1.1213989258771946 2312 18-19 1.107854386449489 2312 20-21 1.1045235443642767 2312 22-23 1.1182231941692962 2312 24-25 1.1058807758937377 2312 26-27 1.166292178861731 2312 28-29 1.2165883177964005 2312 30-31 1.176395345040234 2312 32-33 1.2045147787130617 2312 34-35 1.2321085471518387 2312 36-37 1.2778317948280176 2312 38-39 1.270221862605851 2312 40-41 1.3062630702024336 2312 42-43 1.386592617417584 2312 44-45 1.4538434620635385 2312 46-47 1.529299784542225 2312 48-49 1.4355136103893855 2312 50-51 1.4189348715913113 2312 52-53 1.335978047622639 2312 54-55 1.3457726421770317 2312 56-57 1.2894766673313214 2312 58-59 1.2547027248596052 2312 60-61 1.248687568864291 2312 62-63 1.305800695297897 2312 64-65 1.3232474480094893 2312 66-67 1.2976578891277981 2312 68-69 1.2715105076215067 2312 70-71 1.2225382538384153 2312 72-73 1.2376293984862485 2312 74-75 1.2984986877945488 2312 76-77 1.288847852357243 2312 78-79 1.2461232975600964 2312 80-81 1.3177941008609544 2312 82-83 1.2479243599424876 2312 84-85 1.2802433301520573 2312 86-87 1.3641665068479796 2312 88-89 1.2104765849789878 2312 90-91 1.2758488750078527 2312 92-93 1.2942706849757393 2312 94-95 1.428717679275458 2312 96-97 1.424903555322505 2312 98-99 1.1436108970508165 2312 100 1.2430162375072307 2313 1 0.665557661995944 2313 2 0.6674560007009127 2313 3 0.7131976147209471 2313 4 0.617414503223678 2313 5 0.3968134700581132 2313 6 0.597358750915781 2313 7 0.6447405288217851 2313 8 0.5777921123491794 2313 9 0.7781300479760418 2313 10-11 0.8258617083937523 2313 12-13 0.8738677457051338 2313 14-15 1.0900292696954281 2313 16-17 1.0723412990120735 2313 18-19 1.0839337215528957 2313 20-21 1.1119227627929007 2313 22-23 1.1225438067118318 2313 24-25 1.1121067023736728 2313 26-27 1.1503657924139574 2313 28-29 1.188371347600821 2313 30-31 1.1668715800351848 2313 32-33 1.2377610110903063 2313 34-35 1.294555258194812 2313 36-37 1.286141132549126 2313 38-39 1.3876950311485388 2313 40-41 1.3675776246319558 2313 42-43 1.4163881561035012 2313 44-45 1.4151753120317565 2313 46-47 1.4512740001661726 2313 48-49 1.4854935101687516 2313 50-51 1.5168687558888294 2313 52-53 1.435615308757228 2313 54-55 1.2317627883944553 2313 56-57 1.2106688441384676 2313 58-59 1.2195348179002607 2313 60-61 1.1479845285890633 2313 62-63 1.2196217004959138 2313 64-65 1.1665283649534999 2313 66-67 1.2255083808820402 2313 68-69 1.1400901231931044 2313 70-71 1.1980772202196235 2313 72-73 1.19743409913319 2313 74-75 1.2121121465283267 2313 76-77 1.0168717669453287 2313 78-79 1.1668648554674235 2313 80-81 1.2213228581998727 2313 82-83 1.2712971769650352 2313 84-85 1.2969685835083276 2313 86-87 1.3539074477252768 2313 88-89 1.397408636884304 2313 90-91 1.3682397728800346 2313 92-93 1.3576887508670659 2313 94-95 1.4814139135224949 2313 96-97 1.4887867309703857 2313 98-99 1.5744198608143272 2313 100 1.6701431213186062 2314 1 0.617824369721724 2314 2 0.6036672237449636 2314 3 0.5638666080706081 2314 4 0.45544147401233914 2314 5 0.4105061078832932 2314 6 0.4890289259852949 2314 7 0.536988775192043 2314 8 0.4821263724767775 2314 9 0.7116488841399402 2314 10-11 0.667959209894299 2314 12-13 0.7107559994538306 2314 14-15 0.8774989485831881 2314 16-17 0.8681018186944058 2314 18-19 0.8578062961920949 2314 20-21 0.8674671402989134 2314 22-23 0.9248061569885735 2314 24-25 0.8788522858725187 2314 26-27 0.9226664882422497 2314 28-29 0.9646955907618917 2314 30-31 1.0381302611619745 2314 32-33 0.9752574190532428 2314 34-35 1.0166039376336755 2314 36-37 1.0136095850838842 2314 38-39 0.9829432139144316 2314 40-41 1.0231842260021082 2314 42-43 1.1304882175312443 2314 44-45 1.1745597920771331 2314 46-47 1.067457989365458 2314 48-49 1.0911141366809929 2314 50-51 1.0393714230795794 2314 52-53 1.0352585442050568 2314 54-55 0.9569957086551639 2314 56-57 0.9142424054582747 2314 58-59 0.9000574314516072 2314 60-61 0.9037729274759627 2314 62-63 0.8863448149718138 2314 64-65 0.9073719628530199 2314 66-67 0.9028299420734456 2314 68-69 0.9261521639512225 2314 70-71 0.9897411449540741 2314 72-73 0.9830689489688922 2314 74-75 0.8850074457089221 2314 76-77 0.9191947615638583 2314 78-79 0.9216756655320211 2314 80-81 0.9076154075887395 2314 82-83 0.9393150127788914 2314 84-85 1.0062237248112034 2314 86-87 1.0227291037303843 2314 88-89 1.0165314057454715 2314 90-91 1.1389281913902742 2314 92-93 1.2635489227411156 2314 94-95 1.2306441822183025 2314 96-97 1.2457497566813664 2314 98-99 1.2515673992960252 2314 100 1.2916690296773439 2315 1 0.3356949531285487 2315 2 0.2781691224456466 2315 3 0.40916731308729126 2315 4 0.11878999423720416 2315 5 0.18677769702230762 2315 6 0.16508010720373534 2315 7 0.2289541805115647 2315 8 0.29734602851607406 2315 9 0.422841725588718 2315 10-11 0.3495366876515078 2315 12-13 0.3647362886918799 2315 14-15 0.620992584978346 2315 16-17 0.6125337338402588 2315 18-19 0.4991976009904562 2315 20-21 0.5599570737867126 2315 22-23 0.41202676586313913 2315 24-25 0.5432200092315256 2315 26-27 0.5745978855830671 2315 28-29 0.6092623757681324 2315 30-31 0.6328394225053344 2315 32-33 0.6648467808522156 2315 34-35 0.732497609902012 2315 36-37 0.7937077503085277 2315 38-39 0.8057113508759954 2315 40-41 0.8066316244743845 2315 42-43 0.9346354194781696 2315 44-45 0.8706212541463572 2315 46-47 0.9081616712892 2315 48-49 0.85577868969159 2315 50-51 0.7596152743992803 2315 52-53 0.8457314176979196 2315 54-55 0.692671013382963 2315 56-57 0.5934435929742605 2315 58-59 0.6726700912078769 2315 60-61 0.6165159149648787 2315 62-63 0.6515268646112773 2315 64-65 0.6696153841385737 2315 66-67 0.6207003404397611 2315 68-69 0.651235194712477 2315 70-71 0.5704081987254881 2315 72-73 0.27561953873617995 2315 74-75 0.5390900623495156 2315 76-77 0.5647246207492671 2315 78-79 0.5044188738359523 2315 80-81 0.2655879951940072 2315 82-83 0.3071836902684595 2315 84-85 0.6711019132596725 2315 86-87 0.7651244013328871 2315 88-89 0.7704265393404022 2315 90-91 0.832634457836523 2315 92-93 0.9414469316188665 2315 94-95 0.9852423463943829 2315 96-97 0.7873661077491043 2315 98-99 0.22945037054825335 2315 100 0.780231243936246 2316 1 0.348021539872974 2316 2 0.3122884013714433 2316 3 0.3344102095215149 2316 4 0.2172592887892364 2316 5 0.12313169374708366 2316 6 0.19810956689474324 2316 7 0.1626607256028052 2316 8 0.2327661031503041 2316 9 0.2971849348154265 2316 10-11 0.324579551250757 2316 12-13 0.12992321472400903 2316 14-15 0.19305999616951652 2316 16-17 0.3838800781333802 2316 18-19 0.41776364022995693 2316 20-21 0.377739809001703 2316 22-23 0.3466277515453484 2316 24-25 0.40558504681767005 2316 26-27 0.4777177315329979 2316 28-29 0.34871629103848534 2316 30-31 0.282182232047802 2316 32-33 0.4698332090360182 2316 34-35 0.384074715241816 2316 36-37 0.4681258364223453 2316 38-39 0.6448171188512077 2316 40-41 0.5932226562740723 2316 42-43 0.5961711751496139 2316 44-45 0.5404363776625871 2316 46-47 0.4164979687782875 2316 48-49 0.28141130869787645 2316 50-51 0.4167985700811414 2316 52-53 0.5674506734785538 2316 54-55 0.24050404713570828 2316 56-57 0.1390350676322143 2316 58-59 0.20153806693986098 2316 60-61 0.3570720863452621 2316 62-63 0.4865274290384676 2316 64-65 0.39990336292682827 2316 66-67 0.31906590374256893 2316 68-69 0.31881015904319554 2316 70-71 0.07620802289688555 2316 72-73 0.025259863492408385 2316 74-75 0.28138690933991484 2316 76-77 0.37881946740319705 2316 78-79 0.43232742072442676 2316 80-81 0.6042210807555755 2316 82-83 0.6303480622606301 2316 84-85 0.671871741635659 2316 86-87 0.754333819403179 2316 88-89 0.7239117940756081 2316 90-91 0.5914612713662528 2316 92-93 0.5810422133808437 2316 94-95 0.8241329341103878 2316 96-97 0.6960534943716716 2316 98-99 0.6425086188665183 2316 100 0.724589272724387 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 17.0 1 258.0 2 92.0 3 94.0 4 82.0 5 51.0 6 57.0 7 493.0 8 1670.0 9 2626.0 10 3609.0 11 4924.0 12 6813.0 13 8474.0 14 10000.0 15 11729.0 16 14417.0 17 17995.0 18 21838.0 19 26785.0 20 32483.0 21 38840.0 22 43534.0 23 44298.0 24 43388.0 25 43859.0 26 46990.0 27 53817.0 28 63370.0 29 75193.0 30 91140.0 31 112587.0 32 142314.0 33 187375.0 34 263020.0 35 366336.0 36 546339.0 37 793348.0 38 756762.0 39 209282.0 40 252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.50057354682089 19.367543738301052 12.922836222695317 23.209046492182747 2 15.736451317758112 20.031275896829726 41.23939194795126 22.9928808374609 3 19.344920217999338 26.14371822597019 29.283968903669315 25.227392652361154 4 12.521464164390537 16.60060990533251 38.88481795843246 31.9931079718445 5 13.086501579146262 37.49336719409303 34.598844961710846 14.82128626504986 6 29.870192191161394 37.121375528440616 18.67566116437989 14.332771116018101 7 27.052083950547157 32.33853563990525 22.653563207020984 17.955817202526607 8 25.753760570618134 35.222091621775995 20.453743653222276 18.570404154383603 9 26.27798391682912 16.281021941589138 21.33753385341249 36.10346028816925 10-11 25.15538130077937 26.231452959900864 27.9252309268826 20.687934812437163 12-13 25.516159593427467 24.10064677542547 28.225431346820468 22.15776228432659 14-15 22.84161828546366 25.40865504049287 26.45272715302166 25.296999521021807 16-17 21.747615777305793 28.049352663285543 27.364092086333752 22.838939473074916 18-19 21.32323157415287 27.38920653195207 29.735514729835334 21.552047164059726 20-21 22.47993208488606 26.726560284759838 29.45470512497465 21.338802505379448 22-23 22.453719823175657 26.616772611881927 29.132839402343418 21.79666816259899 24-25 21.970905774567115 26.57521515447236 29.703151568465763 21.750727502494765 26-27 21.899207540723125 26.83812713515863 29.192204538590357 22.07046078552789 28-29 21.533171724499404 27.133065894331615 29.422169486622114 21.91159289454687 30-31 22.236629563276455 26.870294771261026 29.34704396604893 21.54603169941359 32-33 22.196598457466767 27.008662339127724 28.948577411930682 21.846161791474824 34-35 22.25213336680031 27.023123421062582 29.221235604539288 21.503507607597818 36-37 22.193294182983866 26.836081306691256 28.948712030520728 22.02191247980415 38-39 22.137433166205504 27.056767575651143 29.12429831399554 21.681500944147814 40-41 22.18106915807513 26.450385448948804 29.46120856611963 21.907336826856437 42-43 22.0070205980543 26.67666928298033 28.991182823046096 22.325127295919273 44-45 22.05462243657826 27.202182478797198 28.893167795071427 21.850027289553115 46-47 22.046139900860357 26.971466903162273 28.444533395517478 22.537859800459895 48-49 22.32630931123979 26.9219771971836 28.525099793533997 22.226613698042613 50-51 22.262124358571427 27.083070201977183 28.30708383879031 22.34772160066108 52-53 22.00082718665593 27.509231797211726 28.079718859353907 22.41022215677843 54-55 21.977888955187343 27.338884044001777 28.18377931794189 22.49944768286899 56-57 22.407146175044815 27.17436366006464 27.938601940155188 22.479888224735355 58-59 22.00081889405237 27.333067712990488 28.339511760432483 22.32660163252466 60-61 22.194557266336304 27.630205057085945 27.97557711314438 22.199660563433373 62-63 22.246931584316812 27.29088463187824 27.921554690054766 22.540629093750184 64-65 21.90662586719579 27.22538590954545 28.06540333209277 22.80258489116599 66-67 22.039708403270218 27.105554129007636 27.94786879860649 22.906868669115653 68-69 21.85695138893139 27.15814662268435 28.05574085520719 22.92916113317707 70-71 21.947436998267403 26.937953919397195 28.07712292138806 23.03748616094734 72-73 22.194744730868358 27.086075489181425 28.05580257824363 22.663377201706588 74-75 22.31816994119976 27.22597368583697 27.799571282670115 22.656285090293157 76-77 22.162749245692755 27.1369978602211 27.733064264958106 22.96718862912804 78-79 22.094317890338484 27.14302882026641 27.78582610330183 22.976827186093278 80-81 21.953957000409126 27.364794536256497 27.852537864848326 22.828710598486055 82-83 22.450215855810743 26.96926742415982 28.364764196021085 22.21575252400835 84-85 22.548686453573325 27.156937737800256 27.685985758507947 22.60839005011847 86-87 21.971899547926267 27.473596399954626 27.977139212596537 22.577364839522573 88-89 22.40374673579558 26.83675019056872 27.934492087405722 22.82501098622998 90-91 22.483693660860943 26.898551306379524 27.888019157638595 22.72973587512094 92-93 22.534663846778344 26.985716911571245 27.91084897451894 22.56877026713147 94-95 21.968834924817635 26.94861879494292 28.165637401027364 22.91690887921208 96-97 22.18416530271222 26.904047365016236 27.9397348751587 22.97205245711284 98-99 21.970695247141286 27.109154409438478 27.726798037864402 23.19335230555583 100 22.47623187788542 26.800964113996912 27.371237494214167 23.351566513903503 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1104.0 1 804.5 2 1314.0 3 1951.0 4 1308.0 5 911.0 6 1248.5 7 2242.5 8 3549.5 9 4215.0 10 3813.0 11 3259.5 12 3321.0 13 3756.0 14 4243.0 15 4637.5 16 5126.0 17 5645.0 18 6104.5 19 6616.5 20 7260.5 21 8055.0 22 9049.0 23 10541.5 24 12474.0 25 14838.0 26 18249.0 27 22291.5 28 26776.0 29 33576.0 30 42969.0 31 52361.5 32 63264.0 33 75319.5 34 86164.0 35 97021.0 36 107803.0 37 121442.5 38 128735.5 39 128008.5 40 126429.0 41 124013.0 42 124845.0 43 129260.0 44 136092.5 45 147230.5 46 156859.5 47 163066.0 48 168696.0 49 168781.5 50 163726.0 51 161942.5 52 158691.0 53 151149.0 54 142508.0 55 127680.5 56 113130.0 57 96604.0 58 80442.5 59 71160.0 60 58546.0 61 44694.5 62 34931.0 63 27683.0 64 20781.5 65 14109.0 66 10036.0 67 7473.0 68 5832.5 69 4463.5 70 3505.0 71 2757.0 72 2077.5 73 1643.0 74 1296.0 75 958.5 76 724.5 77 522.5 78 350.0 79 243.0 80 180.0 81 127.5 82 91.0 83 73.0 84 65.0 85 57.0 86 39.0 87 30.5 88 31.0 89 25.5 90 22.0 91 16.5 92 11.5 93 7.0 94 5.0 95 6.5 96 5.0 97 3.5 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05667370846466861 2 0.13343770822877288 3 0.01632183227371933 4 0.016419714326335336 5 0.01955194001004759 6 0.05207325199171624 7 0.057187589240902656 8 0.04889208528169599 9 0.04062105183564331 10-11 0.039152821046403184 12-13 0.040890227480337334 14-15 0.018842295128581536 16-17 0.01793688614188346 18-19 0.017814533576113453 20-21 0.02110581759532672 22-23 0.025143452265737047 24-25 0.02442157212769399 26-27 0.019307234878507572 28-29 0.018585354740464514 30-31 0.016419714326335336 32-33 0.022855459285837862 34-35 0.022794283002952855 36-37 0.022855459285837862 38-39 0.020946759259825705 40-41 0.024238043279038977 42-43 0.031481315172623564 44-45 0.040486464013296294 46-47 0.046701974354412805 48-49 0.03956881977002122 50-51 0.02787191448240827 52-53 0.03965446656606023 54-55 0.03642435882973197 56-57 0.038381999882052126 58-59 0.043398455078622535 60-61 0.02341828108837991 62-63 0.025816391377472103 64-65 0.025461568936739072 66-67 0.03230107736328263 68-69 0.03856552873070714 70-71 0.04021728836860228 72-73 0.043887865341702574 74-75 0.027125563831211206 76-77 0.020763230411170692 78-79 0.019943468220511625 80-81 0.024482748410578993 82-83 0.02049405476647667 84-85 0.013030548254506063 86-87 0.014743484175286202 88-89 0.018463002174694505 90-91 0.021289346443981734 92-93 0.019821115654741615 94-95 0.023295928522609897 96-97 0.024568395206618004 98-99 0.027027681778595204 100 0.027945326021870276 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4086551.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.71199916970183 #Duplication Level Percentage of deduplicated Percentage of total 1 83.82144058257764 62.62467399208751 2 11.28608788941523 16.864123780463448 3 2.6559891615865845 5.953027801055819 4 0.9212151114335237 2.753032905621528 5 0.4291521477466684 1.6031407453062423 6 0.24087131213205007 1.0797586363208826 7 0.13407962992322392 0.7012150039648483 8 0.10539005630481695 0.629912143931225 9 0.07449380866893858 0.5009023234279499 >10 0.30614277856991085 3.8883352527213337 >50 0.016987309596331157 0.864217319807065 >100 0.00742459180494813 1.0294944634050316 >500 4.353721654919078E-4 0.2167146284972749 >1k 1.8140504663451923E-4 0.31874259820462486 >5k 3.6281009326903847E-5 0.2015350980229089 >10k+ 7.256201865380769E-5 0.7711733071623077 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15836 0.3875150463067756 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12614 0.30867105292458114 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7435 0.18193826530000481 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 1.2235256577000996E-5 12-13 0.0 0.0 0.0 0.0 2.4470513154001992E-5 14-15 0.0 0.0 0.0 2.4470513154001992E-5 2.4470513154001992E-5 16-17 0.0 1.2235256577000996E-5 0.0 3.6705769731002985E-5 2.4470513154001992E-5 18-19 0.0 2.4470513154001992E-5 0.0 6.117628288500498E-5 2.4470513154001992E-5 20-21 0.0 2.4470513154001992E-5 0.0 2.0799936180901694E-4 2.4470513154001992E-5 22-23 0.0 2.4470513154001992E-5 0.0 3.792929538870309E-4 2.4470513154001992E-5 24-25 0.0 2.4470513154001992E-5 0.0 8.075269340820659E-4 2.4470513154001992E-5 26-27 0.0 3.6705769731002985E-5 0.0 0.0016150538681641317 2.4470513154001992E-5 28-29 0.0 4.8941026308003984E-5 0.0 0.004380221854566357 2.4470513154001992E-5 30-31 0.0 4.8941026308003984E-5 0.0 0.014572190583208189 3.6705769731002985E-5 32-33 0.0 6.117628288500498E-5 0.0 0.03879799860567016 4.8941026308003984E-5 34-35 0.0 7.341153946200598E-5 0.0 0.07787740811261135 4.8941026308003984E-5 36-37 0.0 7.341153946200598E-5 0.0 0.13842969291218926 4.8941026308003984E-5 38-39 0.0 9.788205261600797E-5 0.0 0.2307447037856618 8.564679603900697E-5 40-41 0.0 1.2235256577000997E-4 0.0 0.33240745068396305 1.2235256577000997E-4 42-43 0.0 1.2235256577000997E-4 0.0 0.4279280987806099 1.2235256577000997E-4 44-45 0.0 1.2235256577000997E-4 0.0 0.540675988137674 1.2235256577000997E-4 46-47 0.0 1.2235256577000997E-4 0.0 0.6776252150040462 1.2235256577000997E-4 48-49 0.0 1.2235256577000997E-4 0.0 0.8241424125136332 1.2235256577000997E-4 50-51 0.0 1.2235256577000997E-4 0.0 0.9778417056339197 1.2235256577000997E-4 52-53 0.0 1.2235256577000997E-4 0.0 1.1266224256102517 1.2235256577000997E-4 54-55 0.0 1.2235256577000997E-4 0.0 1.2916270958076872 1.2235256577000997E-4 56-57 0.0 1.2235256577000997E-4 0.0 1.4798420477316934 1.2235256577000997E-4 58-59 0.0 1.2235256577000997E-4 0.0 1.6962103250393792 1.2235256577000997E-4 60-61 0.0 1.2235256577000997E-4 0.0 1.9111226068144016 1.2235256577000997E-4 62-63 0.0 1.2235256577000997E-4 0.0 2.1302682873650665 1.2235256577000997E-4 64-65 0.0 1.2235256577000997E-4 0.0 2.356987591736895 1.2235256577000997E-4 66-67 0.0 1.2235256577000997E-4 0.0 2.6082385855456103 1.3458782234701097E-4 68-69 0.0 1.2235256577000997E-4 0.0 2.883972327764905 1.4682307892401197E-4 70-71 0.0 1.3458782234701097E-4 0.0 3.1755751977645694 1.4682307892401197E-4 72-73 0.0 1.4682307892401197E-4 0.0 3.4656241901789553 1.7129359207801396E-4 74-75 0.0 1.4682307892401197E-4 0.0 3.7515988421531996 1.7129359207801396E-4 76-77 0.0 1.4682307892401197E-4 0.0 4.07014374713542 1.7129359207801396E-4 78-79 0.0 1.4682307892401197E-4 0.0 4.421038670507232 1.7129359207801396E-4 80-81 0.0 1.4682307892401197E-4 0.0 4.7870686062647945 1.9576410523201594E-4 82-83 0.0 1.4682307892401197E-4 0.0 5.162960158823418 2.5694038811702093E-4 84-85 0.0 1.5905833550101297E-4 0.0 5.538031949191384 2.6917564469402193E-4 86-87 0.0 1.7129359207801396E-4 0.0 5.9364363738516905 2.6917564469402193E-4 88 0.0 1.7129359207801396E-4 0.0 6.252362933926434 2.6917564469402193E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7575 0.0 50.763943 1 GTATCAA 13270 0.0 39.93773 1 TACCTGG 3060 0.0 35.53092 2 ATCAACG 15435 0.0 33.011806 3 CAACGCA 15545 0.0 32.808846 5 TATCAAC 16375 0.0 31.847425 2 GTGGTAT 2915 0.0 31.647062 1 AACGCAG 17000 0.0 30.471598 6 TCAACGC 16850 0.0 30.37945 4 GTACCTG 3820 0.0 29.201212 1 GTACTAG 2390 0.0 28.94912 1 ACCTGGG 3705 0.0 27.911366 3 ACGCAGA 18515 0.0 27.496195 7 CGCAGAG 18765 0.0 27.103495 8 GTACATG 14935 0.0 26.818855 1 GCATCGT 2220 0.0 26.678669 8 TGGTATC 3450 0.0 26.466623 2 TACATGG 15025 0.0 26.125673 2 GCAGAGT 20225 0.0 24.589767 9 ACATGGG 15290 0.0 24.409542 3 >>END_MODULE