FastQCFastQC Report
Mon 6 Jun 2016
ERR1211266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4086551
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143180.35036880733900055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124330.3042418900437068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76470.18712601408865326No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74500.051.7578471
TACCTGG34800.039.9762842
GTATCAA133550.039.893731
ACCTGGG40800.033.5259673
ATCAACG157500.032.8590663
CAACGCA159050.032.557245
GTACCTG44200.032.129451
TATCAAC167300.031.6043282
AACGCAG174800.030.3495436
TCAACGC172600.030.1750134
GTGGTAT29350.029.6402781
ACGCAGA192400.027.0359847
CGCAGAG193400.026.8475978
GTACTAG23200.026.146911
GTACATG153550.026.0920621
TGGTATC33550.025.9160732
CCTGGGG38350.025.3719274
TACATGG153750.025.1579172
GCAGAGT210450.024.4045529
ACATGGG154350.024.0889763
Produced by FastQC (version 0.11.5)