##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211266_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4086551 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.141018428498754 33.0 31.0 34.0 30.0 34.0 2 32.27683100002912 34.0 31.0 34.0 30.0 34.0 3 32.354748295078174 34.0 31.0 34.0 30.0 34.0 4 35.97326987966136 37.0 35.0 37.0 35.0 37.0 5 35.85890473408995 37.0 35.0 37.0 35.0 37.0 6 35.89141307669964 37.0 35.0 37.0 35.0 37.0 7 35.83492681236574 37.0 35.0 37.0 35.0 37.0 8 35.82184536544387 37.0 35.0 37.0 35.0 37.0 9 37.42811089351387 39.0 37.0 39.0 34.0 39.0 10-11 37.40705817693208 39.0 37.0 39.0 34.0 39.0 12-13 37.310043359302256 39.0 37.0 39.0 34.0 39.0 14-15 38.52932839942534 40.0 38.0 41.0 34.0 41.0 16-17 38.48192583427932 40.0 38.0 41.0 34.0 41.0 18-19 38.51707760407248 40.0 38.0 41.0 34.0 41.0 20-21 38.481123446153006 40.0 38.0 41.0 34.0 41.0 22-23 38.35379088625102 40.0 38.0 41.0 34.0 41.0 24-25 38.28964633012043 40.0 38.0 41.0 34.0 41.0 26-27 38.146727276865015 40.0 38.0 41.0 33.5 41.0 28-29 38.122008020944804 40.0 37.5 41.0 33.5 41.0 30-31 38.124147110852164 40.0 37.5 41.0 33.5 41.0 32-33 37.9815377319407 40.0 37.0 41.0 33.0 41.0 34-35 37.8286234528824 40.0 37.0 41.0 33.0 41.0 36-37 37.67752011415005 40.0 37.0 41.0 33.0 41.0 38-39 37.582999331220876 40.0 37.0 41.0 33.0 41.0 40-41 37.48179883231605 40.0 36.0 41.0 32.0 41.0 42-43 37.33880440988011 40.0 36.0 41.0 32.0 41.0 44-45 37.19861418589906 40.0 36.0 41.0 31.0 41.0 46-47 36.95878309116905 39.0 36.0 41.0 31.0 41.0 48-49 36.7238394920313 39.0 35.0 41.0 30.0 41.0 50-51 36.52581406667872 39.0 35.0 41.0 30.0 41.0 52-53 36.32916290534487 39.0 35.0 40.0 29.5 41.0 54-55 36.10297155229434 38.5 35.0 40.0 29.0 41.0 56-57 35.85738511522308 38.0 35.0 40.0 28.5 41.0 58-59 35.59763612395881 38.0 34.0 40.0 28.0 41.0 60-61 35.341447470005875 37.5 34.0 40.0 28.0 41.0 62-63 35.05002519239329 37.0 34.0 40.0 27.5 41.0 64-65 34.686165546447356 36.5 34.0 39.5 26.5 41.0 66-67 34.387221767206626 36.0 33.0 39.0 26.0 40.5 68-69 34.056041879815034 35.5 33.0 39.0 26.0 40.0 70-71 33.72864415493652 35.0 33.0 38.0 26.0 40.0 72-73 33.38276201618431 35.0 32.5 37.5 26.0 40.0 74-75 33.0413050026783 35.0 32.0 37.0 25.0 39.0 76-77 32.0423869664174 34.0 30.5 36.0 24.5 38.5 78-79 32.532270978632106 35.0 32.0 36.0 25.0 38.5 80-81 32.452512889231045 35.0 32.0 36.0 25.0 37.5 82-83 32.19193691697473 35.0 32.0 35.0 25.0 37.0 84-85 31.93195875935477 34.0 31.5 35.0 24.5 37.0 86-87 31.687492704728264 34.0 31.0 35.0 24.0 36.0 88-89 31.463186682363684 34.0 31.0 35.0 24.0 36.0 90-91 31.2444664216842 34.0 31.0 35.0 23.0 35.5 92-93 31.065337738351975 34.0 31.0 35.0 23.0 35.0 94-95 30.878582207832473 34.0 30.5 35.0 20.5 35.0 96-97 30.704173152372256 34.0 30.0 35.0 20.0 35.0 98-99 30.526886242212566 34.0 30.0 35.0 20.0 35.0 100 28.765337811763516 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.34063548656718723 1101 2 -0.2891008311655767 1101 3 -0.37884606510989016 1101 4 -0.1975539357442031 1101 5 -0.30287090143523443 1101 6 -0.27452431332768157 1101 7 -0.26936993400285303 1101 8 -0.2745841439777905 1101 9 -0.4510572526964509 1101 10-11 -0.44853199958878776 1101 12-13 -0.49847960516427037 1101 14-15 -0.7380441699930813 1101 16-17 -0.5589267178594142 1101 18-19 -0.7171342807320684 1101 20-21 -0.6923241692828199 1101 22-23 -0.7068720490588305 1101 24-25 -0.7077106330423746 1101 26-27 -0.7738693421827989 1101 28-29 -0.7974502427409647 1101 30-31 -0.6396324430106617 1101 32-33 -0.5833989914199194 1101 34-35 -0.5727774895657873 1101 36-37 -0.5371699368004172 1101 38-39 -0.5738251794552625 1101 40-41 -0.5969246726340813 1101 42-43 -0.5045716221964227 1101 44-45 -0.6382571021093071 1101 46-47 -0.5983466011817882 1101 48-49 -0.6304000048442973 1101 50-51 -0.7369427532160202 1101 52-53 -0.6547884561590394 1101 54-55 -0.5813314064512767 1101 56-57 -0.7377269297183844 1101 58-59 -0.7034510777327085 1101 60-61 -0.8385956198337183 1101 62-63 -0.8201575482998749 1101 64-65 -0.7832833497114606 1101 66-67 -0.7927477991879641 1101 68-69 -1.0143797258097393 1101 70-71 -0.9133833487614069 1101 72-73 -0.9638371061156548 1101 74-75 -0.977694447696674 1101 76-77 -0.9487257116687644 1101 78-79 -0.6864189384069483 1101 80-81 -0.576070965857479 1101 82-83 -0.7213385705716036 1101 84-85 -0.6335599759451362 1101 86-87 -0.7542324848114248 1101 88-89 -0.7672334649404711 1101 90-91 -0.715828748673303 1101 92-93 -0.7236501099233337 1101 94-95 -0.852990565151778 1101 96-97 -1.0079513266045304 1101 98-99 -1.0295376855089344 1101 100 -0.8738155867310056 1102 1 -0.4716329600088578 1102 2 -0.3323011828937332 1102 3 -0.3182596129869495 1102 4 -0.23715056892542918 1102 5 -0.2933218274686098 1102 6 -0.36121691399466016 1102 7 -0.32268297823021896 1102 8 -0.3049543722621664 1102 9 -0.5018554231933052 1102 10-11 -0.49897375357604545 1102 12-13 -0.45350617020542217 1102 14-15 -0.5895734697017687 1102 16-17 -0.6615765232890922 1102 18-19 -0.6143248825708412 1102 20-21 -0.6645543082431686 1102 22-23 -0.6914341557061121 1102 24-25 -0.8142195489345383 1102 26-27 -0.8737393020480226 1102 28-29 -0.7691238392448199 1102 30-31 -0.6833232575308799 1102 32-33 -0.684356743762045 1102 34-35 -0.6978744590239145 1102 36-37 -0.6865506204770995 1102 38-39 -0.8016359293284552 1102 40-41 -0.8109331187836517 1102 42-43 -0.6554409308449181 1102 44-45 -0.6717086167996413 1102 46-47 -0.8002115209938836 1102 48-49 -0.8053350119925895 1102 50-51 -0.9126108781349629 1102 52-53 -0.8947450184866028 1102 54-55 -0.8868212149784611 1102 56-57 -0.939309096787369 1102 58-59 -0.9186762098067618 1102 60-61 -0.8798650374029862 1102 62-63 -0.9870047093382226 1102 64-65 -0.9077214820541215 1102 66-67 -1.065141711207879 1102 68-69 -1.0843331608745501 1102 70-71 -1.1499214405853593 1102 72-73 -1.0396375935738362 1102 74-75 -1.1714574055342197 1102 76-77 -0.9792400016048504 1102 78-79 -0.7160765513975527 1102 80-81 -0.6937334106105624 1102 82-83 -0.7852969362462332 1102 84-85 -0.9178305555149606 1102 86-87 -0.8730928911030205 1102 88-89 -0.9733828943946428 1102 90-91 -0.9726691188558654 1102 92-93 -1.068408330563802 1102 94-95 -1.0577160340833416 1102 96-97 -1.1798533823896982 1102 98-99 -1.107962780308398 1102 100 -0.9357763216936377 1103 1 -0.3125822541227983 1103 2 -0.3662536955074742 1103 3 -0.34124143901165027 1103 4 -0.18919188349066474 1103 5 -0.1889107296282404 1103 6 -0.2906395054582944 1103 7 -0.36467831687593844 1103 8 -0.3108831276314987 1103 9 -0.5621698784554781 1103 10-11 -0.4465362994988311 1103 12-13 -0.47801725793225813 1103 14-15 -0.6809584373976705 1103 16-17 -0.6763317251610559 1103 18-19 -0.5864933751652188 1103 20-21 -0.6527938317859423 1103 22-23 -0.6623964419293529 1103 24-25 -0.726190876142411 1103 26-27 -0.6691589059541556 1103 28-29 -0.6716916851436778 1103 30-31 -0.6403199177827972 1103 32-33 -0.6164008655814754 1103 34-35 -0.8099092561377503 1103 36-37 -0.743325232343544 1103 38-39 -0.6897314027879915 1103 40-41 -0.8430680840902411 1103 42-43 -0.7477536618139595 1103 44-45 -0.8318746285116561 1103 46-47 -0.905403778678135 1103 48-49 -0.943426812342615 1103 50-51 -1.0162457017237188 1103 52-53 -1.078716524286051 1103 54-55 -1.0016955040534654 1103 56-57 -1.017585913446716 1103 58-59 -0.9427139300025544 1103 60-61 -0.9785438734024225 1103 62-63 -1.0357429141990266 1103 64-65 -1.0822912896074328 1103 66-67 -1.225271633727381 1103 68-69 -1.1212254449053347 1103 70-71 -1.1489665034340462 1103 72-73 -0.9817104098674392 1103 74-75 -1.0809045932803265 1103 76-77 -1.0845016204347928 1103 78-79 -0.9017667158373186 1103 80-81 -0.9114125788302729 1103 82-83 -0.8438776124949356 1103 84-85 -0.9538788445662831 1103 86-87 -0.831166343022872 1103 88-89 -0.9676828346691657 1103 90-91 -0.8283436593855278 1103 92-93 -0.9274142387782902 1103 94-95 -1.0415622333564514 1103 96-97 -1.020798789426582 1103 98-99 -1.0880997095517557 1103 100 -1.1213677044503356 1104 1 -0.38325838624157527 1104 2 -0.36882225608966834 1104 3 -0.38432020689016966 1104 4 -0.27990863889360185 1104 5 -0.34180892894955406 1104 6 -0.4166027684176896 1104 7 -0.42168761955738177 1104 8 -0.4318227682158522 1104 9 -0.6106431439351923 1104 10-11 -0.5371129746115741 1104 12-13 -0.6500865560456148 1104 14-15 -0.8099910198481339 1104 16-17 -0.7510960639820823 1104 18-19 -0.6974470912871595 1104 20-21 -0.6913245611260663 1104 22-23 -0.708248616718052 1104 24-25 -0.7305155738165183 1104 26-27 -0.8420714986659306 1104 28-29 -0.9434207259818663 1104 30-31 -0.8754708444504828 1104 32-33 -0.8176166387346555 1104 34-35 -0.8208538726665537 1104 36-37 -0.8170695588105019 1104 38-39 -0.8492905183581669 1104 40-41 -0.7450492919536416 1104 42-43 -0.7322413870675746 1104 44-45 -0.8810344421204803 1104 46-47 -0.821703836285451 1104 48-49 -0.7807602630658579 1104 50-51 -0.8479540708741027 1104 52-53 -0.8309353060713391 1104 54-55 -0.934571869976935 1104 56-57 -0.7739218040423239 1104 58-59 -0.7961205392044164 1104 60-61 -0.9654428501974763 1104 62-63 -0.889174768794561 1104 64-65 -0.8623140399967255 1104 66-67 -0.935018240296408 1104 68-69 -0.8883655201323961 1104 70-71 -1.066477636722972 1104 72-73 -0.9457912075037385 1104 74-75 -0.9781393858489835 1104 76-77 -0.823633654265592 1104 78-79 -0.6973991361546936 1104 80-81 -0.7036265337525514 1104 82-83 -0.7503829496102519 1104 84-85 -0.5706079894801874 1104 86-87 -0.7040266025258326 1104 88-89 -0.7971060386035695 1104 90-91 -0.923567244968396 1104 92-93 -0.8566385315134681 1104 94-95 -1.0208506029793938 1104 96-97 -1.0084250304591897 1104 98-99 -1.1163749723851346 1104 100 -0.925156426243003 1105 1 -0.4392137765085451 1105 2 -0.34665719280313567 1105 3 -0.4537069367221349 1105 4 -0.237293659261681 1105 5 -0.26881800365676867 1105 6 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68-69 -0.9340635587953727 1105 70-71 -1.03048910286104 1105 72-73 -0.8869135887505522 1105 74-75 -0.9219339410246548 1105 76-77 -0.7971739039564341 1105 78-79 -0.7806104731736809 1105 80-81 -0.8090199832324387 1105 82-83 -0.7418487012287827 1105 84-85 -0.9147631915242691 1105 86-87 -1.0226370266183586 1105 88-89 -1.002197945019514 1105 90-91 -1.1072749402837374 1105 92-93 -1.0372711240791155 1105 94-95 -0.9313924484729554 1105 96-97 -1.0236283475465555 1105 98-99 -1.0630417451355534 1105 100 -1.1859053623745233 1106 1 -0.3658220789165014 1106 2 -0.34786522405830667 1106 3 -0.3969528235628381 1106 4 -0.2999887367524039 1106 5 -0.27488369658214395 1106 6 -0.32722361780894005 1106 7 -0.42291474398007267 1106 8 -0.4304582625328024 1106 9 -0.4902487499796351 1106 10-11 -0.5339693885522863 1106 12-13 -0.6102879758649706 1106 14-15 -0.6478656095101769 1106 16-17 -0.6559691953746878 1106 18-19 -0.6392780042655062 1106 20-21 -0.6329177656294007 1106 22-23 -0.6046591527633396 1106 24-25 -0.6592360569873819 1106 26-27 -0.7040171187457602 1106 28-29 -0.7176344162119861 1106 30-31 -0.7067952720331974 1106 32-33 -0.6590487987848022 1106 34-35 -0.7159769976693227 1106 36-37 -0.736180021400564 1106 38-39 -0.7424661355042375 1106 40-41 -0.7434853125699945 1106 42-43 -0.7184199502012731 1106 44-45 -0.7705971682647572 1106 46-47 -0.8643364779617855 1106 48-49 -0.8864701421814587 1106 50-51 -0.9637790793753496 1106 52-53 -1.0134076242335581 1106 54-55 -0.9246415940920301 1106 56-57 -0.9699578823893589 1106 58-59 -0.9242711749087675 1106 60-61 -0.9217365093785972 1106 62-63 -1.0462532236346078 1106 64-65 -0.9247695890328131 1106 66-67 -0.9729414412547754 1106 68-69 -0.8714584223691659 1106 70-71 -0.8774620672172233 1106 72-73 -1.1424722068280815 1106 74-75 -1.1959728625366743 1106 76-77 -1.0549380302962987 1106 78-79 -0.8082570619838165 1106 80-81 -0.8384200534687771 1106 82-83 -0.7067366523199823 1106 84-85 -0.7498965420790107 1106 86-87 -0.7809107955408088 1106 88-89 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1107 46-47 -0.8474915122700253 1107 48-49 -0.8816020162343321 1107 50-51 -0.8941276324833396 1107 52-53 -0.9514991071214993 1107 54-55 -0.8120277003943528 1107 56-57 -0.8156590312038858 1107 58-59 -0.8088244431136928 1107 60-61 -0.9162994810276714 1107 62-63 -0.8954357170192111 1107 64-65 -0.8850752364534102 1107 66-67 -0.9100236331100433 1107 68-69 -0.9667253717460085 1107 70-71 -0.8587810754411009 1107 72-73 -0.8402491701150083 1107 74-75 -0.8582025332576109 1107 76-77 -0.9179665998041351 1107 78-79 -0.8560784767463758 1107 80-81 -0.8228780383204537 1107 82-83 -0.8473919095397484 1107 84-85 -0.8008730354302074 1107 86-87 -0.8194269877732587 1107 88-89 -0.7670685748085866 1107 90-91 -0.7919709057403672 1107 92-93 -0.8042595419153677 1107 94-95 -0.8221424934884745 1107 96-97 -0.8654154719002811 1107 98-99 -0.9298044197491038 1107 100 -1.0208353056000696 1108 1 -0.41490522204994207 1108 2 -0.44512049951728017 1108 3 -0.3774436326654751 1108 4 -0.2918779393256443 1108 5 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2311 28-29 0.5865642649043394 2311 30-31 0.7824518458559453 2311 32-33 0.8942971195152083 2311 34-35 0.996892238974759 2311 36-37 1.0257547653988937 2311 38-39 0.9613064564974181 2311 40-41 0.9751557371066539 2311 42-43 1.0080438431676697 2311 44-45 1.0254938453364986 2311 46-47 0.7084309995408944 2311 48-49 0.95531260656211 2311 50-51 1.083103855665847 2311 52-53 1.0063744949292115 2311 54-55 1.0240851948259078 2311 56-57 1.1352913264926556 2311 58-59 1.2409314199482253 2311 60-61 1.24600654540928 2311 62-63 1.2320629929360933 2311 64-65 1.2523731813521124 2311 66-67 1.1974712090083202 2311 68-69 1.1810218915944972 2311 70-71 1.2364589771887253 2311 72-73 1.236239899813441 2311 74-75 1.1991620936655707 2311 76-77 1.1406558848789672 2311 78-79 0.8636494158440726 2311 80-81 0.9152365381873437 2311 82-83 0.9672815698384767 2311 84-85 1.0582727314854807 2311 86-87 1.0714225294254511 2311 88-89 1.0778854843581875 2311 90-91 1.1119027253893883 2311 92-93 1.2698676395465576 2311 94-95 1.2308396568980626 2311 96-97 1.1740870018992595 2311 98-99 1.3185225488953094 2311 100 1.4563270252354386 2312 1 0.28130860493365617 2312 2 0.43555204353212673 2312 3 0.4998604381581586 2312 4 0.22103730941848454 2312 5 0.40710110747011186 2312 6 0.45121091347052555 2312 7 0.34827841209971666 2312 8 0.43987057118742 2312 9 0.6667630929327188 2312 10-11 0.6283640704927862 2312 12-13 0.6921075100000493 2312 14-15 0.9103880160812636 2312 16-17 0.9180671591584186 2312 18-19 0.8784371361956858 2312 20-21 0.8769283892171131 2312 22-23 0.9439669859573954 2312 24-25 0.9279933647603187 2312 26-27 1.03346757368314 2312 28-29 1.0115423290678578 2312 30-31 0.9797067014242842 2312 32-33 0.8292132744184428 2312 34-35 0.9472795756455383 2312 36-37 1.0170154408393088 2312 38-39 0.7543415992353246 2312 40-41 0.9332906366077864 2312 42-43 1.0356110344900742 2312 44-45 0.7233549594063362 2312 46-47 0.7489713283034263 2312 48-49 0.9659291367100238 2312 50-51 0.8663146029597684 2312 52-53 1.0503103165648824 2312 54-55 0.902143268697948 2312 56-57 0.9053743596360633 2312 58-59 1.0291322535536693 2312 60-61 1.0994975301078895 2312 62-63 1.1097317657340824 2312 64-65 1.0920685766162421 2312 66-67 1.12988493241685 2312 68-69 1.1327152072103743 2312 70-71 1.071970062534625 2312 72-73 1.0586531299384845 2312 74-75 1.111150955935578 2312 76-77 1.059221743899414 2312 78-79 0.9417082318240162 2312 80-81 0.8604782078078941 2312 82-83 0.8445211423494072 2312 84-85 0.930559672307183 2312 86-87 0.9217376851895303 2312 88-89 0.931371558048621 2312 90-91 0.9836188542751962 2312 92-93 1.0809638987989025 2312 94-95 1.0618973309049053 2312 96-97 0.7458507784776351 2312 98-99 0.4915660032417044 2312 100 0.6173911119276099 2313 1 0.08275114634945879 2313 2 0.2916241799518531 2313 3 0.3392746335883956 2313 4 0.2367453981212222 2313 5 0.34456137447583046 2313 6 0.25854889634327094 2313 7 0.26990064013905624 2313 8 0.4364180077081201 2313 9 0.6127290253959714 2313 10-11 0.4138461018304298 2313 12-13 0.5074598715422454 2313 14-15 0.5625868867293349 2313 16-17 0.7889723235127732 2313 18-19 0.7746026685243095 2313 20-21 0.7036177426444397 2313 22-23 0.6852579810585482 2313 24-25 0.55437794274917 2313 26-27 0.7830403271329018 2313 28-29 0.6144138559367107 2313 30-31 0.8305322128112991 2313 32-33 0.9129353678330503 2313 34-35 0.9104269897287836 2313 36-37 0.9763251151222434 2313 38-39 0.9396852662634885 2313 40-41 0.9255945893395463 2313 42-43 1.0161600977262992 2313 44-45 0.8683579510670896 2313 46-47 0.8920844317774907 2313 48-49 1.0170598909824875 2313 50-51 0.9711512769809403 2313 52-53 1.0125952909487879 2313 54-55 1.0203096611290476 2313 56-57 0.7579785068257721 2313 58-59 0.7046978644134043 2313 60-61 0.6000561292627467 2313 62-63 0.9668626695895881 2313 64-65 1.1144789202832683 2313 66-67 1.045573855792945 2313 68-69 1.1543785705849672 2313 70-71 1.0994671643632756 2313 72-73 1.161758847750086 2313 74-75 1.2926947463697473 2313 76-77 1.1983045194126731 2313 78-79 1.0358600025132105 2313 80-81 0.8825552617699728 2313 82-83 0.9454948344327221 2313 84-85 0.9210471260299258 2313 86-87 0.9465446295820392 2313 88-89 0.9379812585085965 2313 90-91 0.8834328103725788 2313 92-93 1.0469786327280737 2313 94-95 1.2583554850502239 2313 96-97 1.19369631529376 2313 98-99 1.1852227475668968 2313 100 1.3933091778189386 2314 1 -0.3354260814778769 2314 2 0.029865652415615784 2314 3 0.323881447283604 2314 4 0.2632850152941302 2314 5 0.35111668862843004 2314 6 0.44190582088030084 2314 7 0.4744109259631557 2314 8 0.4922282912370406 2314 9 0.4576728219257191 2314 10-11 0.5647233253468968 2314 12-13 0.4605778909956939 2314 14-15 0.7693155756910599 2314 16-17 0.8431171849370074 2314 18-19 0.8062139710272334 2314 20-21 0.8107884395491709 2314 22-23 0.8368550941151653 2314 24-25 0.796259324940479 2314 26-27 0.717407214348313 2314 28-29 0.7730930729035421 2314 30-31 0.8667313501803662 2314 32-33 0.8753693931326794 2314 34-35 0.91573172871081 2314 36-37 0.9270822780019969 2314 38-39 0.6999580053085239 2314 40-41 0.6875945551236171 2314 42-43 0.8079621142131259 2314 44-45 0.8776877618538634 2314 46-47 0.9325851420543927 2314 48-49 0.8716384350389248 2314 50-51 0.9190517064331587 2314 52-53 0.9237844239705666 2314 54-55 0.8574788200109538 2314 56-57 0.8611471260446066 2314 58-59 1.03339227189646 2314 60-61 1.0984416075831902 2314 62-63 1.0215347654090863 2314 64-65 0.9855756474367539 2314 66-67 0.711232867662396 2314 68-69 0.6558174180435046 2314 70-71 0.8966677383777863 2314 72-73 1.0102562519554397 2314 74-75 1.1449898431512722 2314 76-77 1.006549384673491 2314 78-79 0.8687490581341706 2314 80-81 0.9009142871140625 2314 82-83 0.9121777536644231 2314 84-85 0.9005116628737468 2314 86-87 1.034479306297012 2314 88-89 0.9485802557278724 2314 90-91 1.0191320098638776 2314 92-93 0.9286952127242749 2314 94-95 0.8947866216448617 2314 96-97 0.909150467773312 2314 98-99 1.1178973015127518 2314 100 0.96652855009728 2315 1 0.44454804492428224 2315 2 0.48784424766947154 2315 3 0.49429290213008414 2315 4 0.3275812883438576 2315 5 0.41519535403731567 2315 6 0.4094630381818831 2315 7 0.34801382384558366 2315 8 0.22750131789410233 2315 9 0.43919825395521883 2315 10-11 0.5257874711203314 2315 12-13 0.32241245910436334 2315 14-15 0.577195229783733 2315 16-17 0.754227732702553 2315 18-19 0.690710758909006 2315 20-21 0.43902533778663866 2315 22-23 0.8203871671252401 2315 24-25 0.8483227548538537 2315 26-27 0.7689841727584863 2315 28-29 0.7662125882257058 2315 30-31 0.8275849198523701 2315 32-33 0.7679943321772527 2315 34-35 0.7349349985493419 2315 36-37 0.704306208970543 2315 38-39 0.7966462982765066 2315 40-41 0.8092612199398772 2315 42-43 0.9250497665461808 2315 44-45 0.89629247498371 2315 46-47 0.852410666123042 2315 48-49 0.7996738981762022 2315 50-51 0.8767595874195422 2315 52-53 0.9563294723913529 2315 54-55 0.817436176808414 2315 56-57 0.7532632036841491 2315 58-59 0.720756706592141 2315 60-61 0.8763513603653621 2315 62-63 0.945946210140022 2315 64-65 1.046356829260887 2315 66-67 1.0852046129632598 2315 68-69 1.0663685883445524 2315 70-71 0.9872058398843464 2315 72-73 1.0563254795581045 2315 74-75 0.5209109902346754 2315 76-77 0.43083617378022865 2315 78-79 0.7182202678661156 2315 80-81 0.7125085677832672 2315 82-83 0.6663477957784067 2315 84-85 0.7368013884500328 2315 86-87 0.784498889751081 2315 88-89 0.7482667477582403 2315 90-91 0.7902354175329158 2315 92-93 0.8467738310095108 2315 94-95 0.8325046995970027 2315 96-97 0.9459703837575155 2315 98-99 1.055003265454772 2315 100 1.024292152949517 2316 1 0.34667669423418346 2316 2 0.09157014072435743 2316 3 0.24808740697080367 2316 4 0.04844083751463302 2316 5 0.30838300591225476 2316 6 0.3874040386195432 2316 7 0.4294541754963035 2316 8 0.4691594613449581 2316 9 0.6538293429620978 2316 10-11 0.6502889918275869 2316 12-13 0.4209034623945911 2316 14-15 0.5693981611207377 2316 16-17 0.8023248015197453 2316 18-19 0.7569405078009908 2316 20-21 0.7546464101788288 2316 22-23 0.7706529145424881 2316 24-25 0.5556346514777388 2316 26-27 0.7668599828697538 2316 28-29 0.9845968137394792 2316 30-31 0.6760562838355213 2316 32-33 0.7438974298883991 2316 34-35 0.8750386502674914 2316 36-37 0.948301856023626 2316 38-39 0.8510553754668564 2316 40-41 0.823114701944391 2316 42-43 0.8551709207823421 2316 44-45 0.5521696755903847 2316 46-47 0.8015636276778011 2316 48-49 0.8758051445815838 2316 50-51 0.8506862972585978 2316 52-53 0.8962305146299059 2316 54-55 0.7119863664778663 2316 56-57 0.8872796440564983 2316 58-59 0.7879719713328441 2316 60-61 0.9347982628611575 2316 62-63 0.9393314967444581 2316 64-65 0.9430035758270705 2316 66-67 0.9694320744440503 2316 68-69 0.9532689027114287 2316 70-71 0.9984841786132179 2316 72-73 0.9648220930355293 2316 74-75 0.9647452500644889 2316 76-77 0.7634723928662766 2316 78-79 0.5767771667124038 2316 80-81 0.5002843488981554 2316 82-83 0.5007270033680697 2316 84-85 0.5982899002263551 2316 86-87 0.6849264463932379 2316 88-89 0.6800064608799055 2316 90-91 0.5651491567458038 2316 92-93 0.44773662775071443 2316 94-95 0.5065772718682311 2316 96-97 0.6917601464359677 2316 98-99 0.8897120400942882 2316 100 0.5435493456631413 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 4.0 12 9.0 13 88.0 14 397.0 15 1139.0 16 2847.0 17 5782.0 18 9883.0 19 14064.0 20 19728.0 21 26837.0 22 33082.0 23 33995.0 24 33438.0 25 35114.0 26 40606.0 27 49221.0 28 60311.0 29 75386.0 30 93389.0 31 116438.0 32 147262.0 33 194653.0 34 275713.0 35 384575.0 36 597075.0 37 907601.0 38 804481.0 39 123418.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.5968573313634 20.149434052798888 13.787837429264249 23.46587118657346 2 15.474002404472623 20.448735376115458 40.80220704452239 23.275055174889534 3 18.837670201595426 26.100029095440142 29.960521721128647 25.101778981835782 4 12.418634242514816 16.918790052539677 38.77329408830084 31.88928161664467 5 12.900414065553079 37.667534309494734 34.707042687097264 14.72500893785493 6 30.05064662107484 37.41994165740254 18.312141461100083 14.217270260422543 7 26.99106035556939 32.41891136606341 22.52456615855658 18.065462119810615 8 25.833031326416823 35.6519960230522 20.12878341662688 18.38618923390409 9 26.02531232929816 16.255489379531245 21.251926055145383 36.46727223602521 10-11 25.177769688886986 26.428241512527418 27.747194293457483 20.64679450512812 12-13 25.4667230956769 24.133495964383652 28.218591694878114 22.18118924506133 14-15 22.7263656488431 25.504102302518767 26.376158940073235 25.393373108564898 16-17 21.674823325413428 28.181638322996346 27.33105291923715 22.81248543235308 18-19 21.25428019863205 27.452710121567065 29.72195868839028 21.571050991410605 20-21 22.404063964942562 26.7340233854906 29.470707694581566 21.39120495498527 22-23 22.330946071638405 26.637523916867796 29.148369860060473 21.883160151433323 24-25 21.887865838073402 26.61830364653387 29.72808556512998 21.765744950262746 26-27 21.868659815055942 26.85969989719892 29.2041077363134 22.06753255143174 28-29 21.44116589427427 27.143659601979227 29.49217132171904 21.92300318202746 30-31 22.15887609186936 26.774698363251275 29.482558843790034 21.58386670108933 32-33 22.178236625730694 26.951894836271812 29.00870755728159 21.861160980715912 34-35 22.220593141493982 26.977772675681877 29.309146298330152 21.49248788449399 36-37 22.158102596514382 26.829718455143215 29.03672141163592 21.97545753670648 38-39 22.054197205575143 26.98221941020184 29.2601150127214 21.703468371501614 40-41 22.086746796903178 26.437215191236348 29.572956314354975 21.903081697505502 42-43 21.94662143858862 26.615220625139024 29.129262431094027 22.308895505178334 44-45 21.931420267268646 27.143768251006588 29.01066940507225 21.914142076652514 46-47 21.962171810634374 26.88161331874945 28.575692479469694 22.580522391146484 48-49 22.281460472406454 26.882654550104096 28.627642672340748 22.208242305148705 50-51 22.163319022560117 27.050102390247872 28.38927016280264 22.397308424389376 52-53 21.907902280056156 27.40838434054138 28.13725685741594 22.54645652198652 54-55 21.93360093898552 27.251370006500064 28.219111468310214 22.595917586204195 56-57 22.39799057098265 27.049756590793077 27.992707267332168 22.559545570892105 58-59 21.893580687538055 27.283742088065484 28.413768479126983 22.408908745269482 60-61 22.09705735236106 27.63440476038986 27.99018350680356 22.278354380445517 62-63 22.18053208395061 27.271468137265696 27.900344010935523 22.647655767848168 64-65 21.786899351662704 27.208110519661254 28.02388701281375 22.981103115862293 66-67 21.918297593310175 27.105962250494514 27.95309855759725 23.02264159859806 68-69 21.713740323439996 27.123272812392262 28.06483980787915 23.09814705628859 70-71 21.69526121937673 26.874501070972084 28.174749444437442 23.255488265213746 72-73 21.86571791078667 27.04935982847969 28.114335447550857 22.970586813182777 74-75 21.983970616836643 27.17334296517763 27.844434617308604 22.998251800677124 76-77 21.76084973501469 27.0729297384123 27.834760202402485 23.331460324170518 78-79 21.613721378510963 27.066476648075977 27.937077022630913 23.382724950782148 80-81 21.427644159680355 27.337931969436447 27.944991577759115 23.289432293124083 82-83 21.886929448899547 26.89517433978449 28.415730018012443 22.80216619330352 84-85 21.991038311384095 27.04699959222762 27.817396811002094 23.14456528538619 86-87 21.291696012561616 27.368043859875712 28.104040741508378 23.23621938605429 88-89 21.637442652898056 26.780420135245496 28.05637555296258 23.525761658893867 90-91 21.654245252011513 26.766209361604616 28.081875939440508 23.497669446943362 92-93 21.644429081635025 26.876024657635522 28.0806070942723 23.398939166457154 94-95 20.985397440428233 26.795266074660866 28.394741453491392 23.824595031419513 96-97 21.112742123695845 26.822057016431383 28.164087941555664 23.901112918317104 98-99 20.808347398884678 27.006348099877815 28.025676321313327 24.159628179924184 100 21.010858408884122 26.736668591491135 27.902603066027513 24.349869933597226 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1022.0 1 739.0 2 1105.0 3 1677.0 4 1172.0 5 784.5 6 1138.0 7 2103.0 8 3222.0 9 3754.5 10 3350.0 11 2810.5 12 2956.0 13 3306.0 14 3719.5 15 4240.5 16 4787.0 17 5332.5 18 5841.5 19 6495.5 20 7199.0 21 8064.0 22 9218.5 23 10686.5 24 12598.0 25 15087.0 26 18571.5 27 22753.0 28 27296.5 29 34074.5 30 43395.5 31 52899.0 32 63971.5 33 76056.0 34 86911.5 35 97264.0 36 108003.0 37 122307.0 38 130063.5 39 128856.5 40 126542.0 41 124149.0 42 125389.0 43 130355.0 44 137011.0 45 148736.0 46 159195.5 47 165345.5 48 170841.0 49 170022.5 50 164915.5 51 162946.5 52 159101.0 53 150194.5 54 141196.0 55 127187.5 56 112567.0 57 96327.5 58 80234.0 59 70992.0 60 58055.0 61 43416.5 62 33265.0 63 26134.0 64 19316.0 65 12664.0 66 8841.0 67 6620.5 68 5102.0 69 3945.0 70 3019.0 71 2240.5 72 1678.0 73 1364.5 74 1061.5 75 773.5 76 566.0 77 343.5 78 200.0 79 140.0 80 93.0 81 50.0 82 29.0 83 15.5 84 11.0 85 8.5 86 7.0 87 9.0 88 6.0 89 1.5 90 2.5 91 2.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0441937467561276 2 0.0 3 0.0 4 0.035433303046994886 5 0.0 6 0.0 7 8.564679603900696E-4 8 0.0 9 0.011721375800766955 10-11 3.303519275790269E-4 12-13 1.2235256577000996E-5 14-15 1.1011730919300897E-4 16-17 3.058814144250249E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.484685985810529E-4 26-27 4.4046923677203587E-4 28-29 1.3458782234701097E-4 30-31 0.0023491692627841915 32-33 0.015110541872596231 34-35 0.026526036258938162 36-37 0.031934019665972604 38-39 0.022414990049065827 40-41 9.054089866980737E-4 42-43 9.788205261600797E-5 44-45 0.011623493748150946 46-47 0.01864653102334952 48-49 0.003266813506059266 50-51 0.011464435412649934 52-53 0.010901613610107889 54-55 0.010742555274606875 56-57 4.159987236180339E-4 58-59 0.0078060936961266356 60-61 0.003988693644102325 62-63 0.0012969371971621058 64-65 0.036363182546846964 66-67 0.06111510660211998 68-69 0.0600751097930749 70-71 0.053284542392839335 72-73 0.0465796217886428 74-75 0.036473299856039976 76-77 0.03241119467247564 78-79 0.015538775852791264 80-81 0.0012112904011230988 82-83 0.039079409506941186 84-85 0.05296642572183731 86-87 0.05781158732632971 88-89 0.07013249069936972 90-91 0.049075614130351 92-93 0.04037634670410329 94-95 0.013581134800471108 96-97 0.003242342992905264 98-99 0.022488401588527832 100 0.03254578249482265 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4086551.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.68450439818258 #Duplication Level Percentage of deduplicated Percentage of total 1 82.8884230975814 61.075923762921555 2 11.89452530170169 17.52884403815064 3 2.8516768142241604 6.303731782796864 4 0.9685606649535063 2.854716503066932 5 0.4711507465275106 1.735825462735668 6 0.2347486293358592 1.0378401846459269 7 0.15434706199303297 0.7961090737790537 8 0.10998823435408797 0.6483542830409705 9 0.07080923992517626 0.46957893756287544 >10 0.33073338913031686 4.153891557468855 >50 0.016264953119673593 0.8171238907208129 >100 0.008071005019781833 1.1341040829876472 >500 4.057622991557385E-4 0.20697842402146574 >1k 1.8443739658359276E-4 0.3020281194223525 >5k 3.6887479316718546E-5 0.20784818477526368 >10k+ 7.377495863343709E-5 0.7271017119031096 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14318 0.35036880733900055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12433 0.3042418900437068 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7647 0.18712601408865326 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.4470513154001992E-5 5 0.0 0.0 0.0 0.0 2.4470513154001992E-5 6 0.0 0.0 0.0 0.0 2.4470513154001992E-5 7 0.0 0.0 0.0 0.0 2.4470513154001992E-5 8 0.0 0.0 0.0 0.0 2.4470513154001992E-5 9 0.0 0.0 0.0 0.0 2.4470513154001992E-5 10-11 0.0 0.0 0.0 2.4470513154001992E-5 2.4470513154001992E-5 12-13 0.0 0.0 0.0 2.4470513154001992E-5 2.4470513154001992E-5 14-15 0.0 0.0 0.0 6.117628288500498E-5 2.4470513154001992E-5 16-17 0.0 0.0 0.0 7.341153946200598E-5 4.8941026308003984E-5 18-19 0.0 0.0 0.0 8.564679603900697E-5 9.788205261600797E-5 20-21 0.0 0.0 0.0 2.2023461838601794E-4 9.788205261600797E-5 22-23 0.0 0.0 0.0 4.0376346704103287E-4 1.2235256577000997E-4 24-25 0.0 1.2235256577000996E-5 0.0 8.442327038130687E-4 1.4682307892401197E-4 26-27 0.0 2.4470513154001992E-5 0.0 0.0016762301510491366 1.4682307892401197E-4 28-29 0.0 2.4470513154001992E-5 0.0 0.004527044933490368 1.4682307892401197E-4 30-31 0.0 2.4470513154001992E-5 0.0 0.0147434841752862 1.4682307892401197E-4 32-33 0.0 2.4470513154001992E-5 0.0 0.03888364540170917 1.7129359207801396E-4 34-35 0.0 2.4470513154001992E-5 0.0 0.07792634913891935 1.7129359207801396E-4 36-37 0.0 3.6705769731002985E-5 0.0 0.13833181085957327 1.7129359207801396E-4 38-39 0.0 7.341153946200597E-5 0.0 0.2307202332725078 1.7129359207801396E-4 40-41 0.0 9.788205261600797E-5 0.0 0.3323829801708091 1.7129359207801396E-4 42-43 0.0 1.2235256577000997E-4 0.0 0.42860103789234494 1.7129359207801396E-4 44-45 0.0 1.2235256577000997E-4 0.0 0.5419117490519512 1.7129359207801396E-4 46-47 0.0 1.2235256577000997E-4 0.0 0.6800845015760234 1.7129359207801396E-4 48-49 0.0 1.2235256577000997E-4 0.0 0.8270299330658054 1.7129359207801396E-4 50-51 0.0 1.3458782234701097E-4 0.0 0.9808393434952849 1.7129359207801396E-4 52-53 0.0 1.4682307892401197E-4 0.0 1.129032771155921 1.8352884865501494E-4 54-55 0.0 1.4682307892401197E-4 0.0 1.2932910907021593 1.9576410523201594E-4 56-57 0.0 1.4682307892401197E-4 0.0 1.4811512201854327 1.9576410523201594E-4 58-59 0.0 1.4682307892401197E-4 0.0 1.6972503218484243 1.9576410523201594E-4 60-61 0.0 1.4682307892401197E-4 0.0 1.9123461324721018 1.9576410523201594E-4 62-63 0.0 1.4682307892401197E-4 0.0 2.1315652245622285 1.9576410523201594E-4 64-65 0.0 1.4682307892401197E-4 0.0 2.3578807654670157 1.9576410523201594E-4 66-67 0.0 1.4682307892401197E-4 0.0 2.6090338772231156 1.9576410523201594E-4 68-69 0.0 1.4682307892401197E-4 0.0 2.885293735475221 1.9576410523201594E-4 70-71 0.0 1.4682307892401197E-4 0.0 3.1778264849747377 1.9576410523201594E-4 72-73 0.0 1.4682307892401197E-4 0.0 3.4682792408561642 2.2023461838601794E-4 74-75 0.0 1.4682307892401197E-4 0.0 3.754853420402682 2.2023461838601794E-4 76-77 0.0 1.4682307892401197E-4 0.0 4.073997852957175 2.2023461838601794E-4 78-79 0.0 1.4682307892401197E-4 0.0 4.426458889170844 2.2023461838601794E-4 80-81 0.0 1.4682307892401197E-4 0.0 4.79340646917168 2.2023461838601794E-4 82-83 0.0 1.4682307892401197E-4 0.0 5.169811902506539 2.2023461838601794E-4 84-85 0.0 1.4682307892401197E-4 0.0 5.544541105690349 2.2023461838601794E-4 86-87 0.0 1.4682307892401197E-4 0.0 5.942847648298039 2.2023461838601794E-4 88 0.0 1.4682307892401197E-4 0.0 6.258969972478014 2.2023461838601794E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7450 0.0 51.757847 1 TACCTGG 3480 0.0 39.976284 2 GTATCAA 13355 0.0 39.89373 1 ACCTGGG 4080 0.0 33.525967 3 ATCAACG 15750 0.0 32.859066 3 CAACGCA 15905 0.0 32.55724 5 GTACCTG 4420 0.0 32.12945 1 TATCAAC 16730 0.0 31.604328 2 AACGCAG 17480 0.0 30.349543 6 TCAACGC 17260 0.0 30.175013 4 GTGGTAT 2935 0.0 29.640278 1 ACGCAGA 19240 0.0 27.035984 7 CGCAGAG 19340 0.0 26.847597 8 GTACTAG 2320 0.0 26.14691 1 GTACATG 15355 0.0 26.092062 1 TGGTATC 3355 0.0 25.916073 2 CCTGGGG 3835 0.0 25.371927 4 TACATGG 15375 0.0 25.157917 2 GCAGAGT 21045 0.0 24.404552 9 ACATGGG 15435 0.0 24.088976 3 >>END_MODULE