FastQCFastQC Report
Mon 6 Jun 2016
ERR1211265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8420031
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235090.27920324758899345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191350.22725569537689352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116190.13799236606136012No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132950.049.205681
GTATCAA234600.040.764721
GTGGTAT44750.033.759871
CAACGCA272050.033.4535265
ATCAACG279550.032.589643
TATCAAC295750.031.9225482
GTACTAG60900.031.916961
AACGCAG306200.030.4287226
TCAACGC314950.029.344534
GCATCGT60400.028.794628
CTAACGC29100.028.1087473
TAACGCC30150.027.4416754
TCTAACG30450.027.2029952
ACGCAGA333850.027.1904347
CGCAGAG340950.026.6218458
CTGTGCG87150.025.8350649
TAGTACT84800.025.7775924
TAGGCAT81000.025.5360055
GTACATG315700.025.462561
TACATGG316700.025.0107922
Produced by FastQC (version 0.11.5)