##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211265_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8420031 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.44979870026607 31.0 31.0 34.0 29.0 34.0 2 31.469049460744266 31.0 31.0 34.0 28.0 34.0 3 31.530727974754488 31.0 31.0 34.0 28.0 34.0 4 35.13111745075523 37.0 35.0 37.0 32.0 37.0 5 35.18353186585655 37.0 35.0 37.0 32.0 37.0 6 35.24782070279789 37.0 35.0 37.0 32.0 37.0 7 35.16151923906219 37.0 35.0 37.0 32.0 37.0 8 35.16790377612624 37.0 35.0 37.0 32.0 37.0 9 36.7325386331713 39.0 37.0 39.0 32.0 39.0 10-11 36.68212213232944 39.0 37.0 39.0 32.0 39.0 12-13 36.580020073560306 39.0 37.0 39.0 32.0 39.0 14-15 37.77444697056341 40.0 37.0 41.0 32.0 41.0 16-17 37.7535374275938 40.0 37.0 41.0 32.0 41.0 18-19 37.76032309144705 40.0 37.0 41.0 32.0 41.0 20-21 37.72771454166855 40.0 37.0 41.0 32.0 41.0 22-23 37.61997028276974 40.0 37.0 41.0 32.0 41.0 24-25 37.5256999647626 40.0 37.0 41.0 31.5 41.0 26-27 37.375224924943865 40.0 37.0 41.0 31.0 41.0 28-29 37.257289670311195 40.0 36.0 41.0 31.0 41.0 30-31 37.10995612723991 39.5 36.0 41.0 30.5 41.0 32-33 36.93665023323548 39.0 36.0 41.0 30.0 41.0 34-35 36.792581583131934 39.0 35.5 41.0 30.0 41.0 36-37 36.69127091099783 39.0 35.0 41.0 30.0 41.0 38-39 36.54923651706271 39.0 35.0 41.0 30.0 41.0 40-41 36.38180708598341 39.0 35.0 40.0 29.0 41.0 42-43 36.166952235686544 39.0 35.0 40.0 28.5 41.0 44-45 35.941539526398415 38.0 35.0 40.0 28.0 41.0 46-47 35.7095354518291 38.0 34.5 40.0 27.0 41.0 48-49 35.50120504306932 38.0 34.0 40.0 26.5 41.0 50-51 34.81550744884431 37.5 33.5 39.5 25.5 40.5 52-53 34.91995955834367 38.0 33.5 39.5 26.0 40.5 54-55 35.39382948827623 38.0 34.0 40.0 27.0 41.0 56-57 35.34145444357628 38.0 34.0 40.0 26.5 41.0 58-59 35.116480806305816 37.5 34.0 40.0 26.0 41.0 60-61 34.880987314654774 37.0 34.0 40.0 26.0 41.0 62-63 34.58191038726579 36.5 33.5 40.0 26.0 41.0 64-65 34.32558906255808 36.0 33.0 39.5 26.0 41.0 66-67 34.14996512483149 36.0 33.0 39.0 26.0 41.0 68-69 33.85596781056982 35.0 33.0 39.0 25.0 41.0 70-71 33.56139407325223 35.0 33.0 38.5 25.0 40.5 72-73 33.233904542631734 35.0 32.0 37.5 24.5 40.0 74-75 32.91070982992818 35.0 32.0 37.0 24.0 39.5 76-77 32.59574359049272 35.0 32.0 36.5 24.0 39.0 78-79 32.277758894236854 35.0 31.5 36.0 23.0 39.0 80-81 31.9944910535365 35.0 31.0 36.0 23.0 37.5 82-83 31.71501161931589 35.0 31.0 35.0 22.0 37.0 84-85 31.4823679390254 34.5 31.0 35.0 21.0 37.0 86-87 31.271540508580074 34.0 31.0 35.0 20.0 36.0 88-89 31.068718452461756 34.0 31.0 35.0 20.0 36.0 90-91 30.853385100363646 34.0 30.0 35.0 20.0 36.0 92-93 30.664581698095887 34.0 30.0 35.0 19.0 35.0 94-95 30.504793034609968 34.0 30.0 35.0 19.0 35.0 96-97 30.334006430617656 34.0 29.5 35.0 18.0 35.0 98-99 30.106812136439878 34.0 29.0 35.0 18.0 35.0 100 28.963276382236597 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.41607140352699545 1101 2 -0.3850617405075418 1101 3 -0.4599190414802976 1101 4 -0.2827250280583584 1101 5 -0.22740009658164695 1101 6 -0.266287714718743 1101 7 -0.30106611276610096 1101 8 -0.2554029222020233 1101 9 -0.506058177277005 1101 10-11 -0.4633251661924689 1101 12-13 -0.4952346030023165 1101 14-15 -0.6045000535310976 1101 16-17 -0.6340354128420884 1101 18-19 -0.6188532284822941 1101 20-21 -0.6948016307746059 1101 22-23 -0.7373801012139936 1101 24-25 -0.8347730225442689 1101 26-27 -0.7569981158390462 1101 28-29 -0.7759160143214388 1101 30-31 -0.8712881340282479 1101 32-33 -0.8466145419825963 1101 34-35 -0.857822907345799 1101 36-37 -0.8793440724806842 1101 38-39 -0.8951459793112022 1101 40-41 -0.95370209775691 1101 42-43 -0.9468510872372278 1101 44-45 -0.8708562257962953 1101 46-47 -1.120029766097943 1101 48-49 -1.095209133971096 1101 50-51 -1.2096503566408785 1101 52-53 -1.0652117967331307 1101 54-55 -0.8904726546433679 1101 56-57 -0.7912408163980231 1101 58-59 -0.7724079527970815 1101 60-61 -0.7223622495503861 1101 62-63 -0.8564600940882059 1101 64-65 -0.874816256248856 1101 66-67 -0.9790903781976752 1101 68-69 -0.9199898036181509 1101 70-71 -0.9799106840814673 1101 72-73 -1.037176237561134 1101 74-75 -1.0035409846745758 1101 76-77 -1.0658532709374597 1101 78-79 -1.1172991257214306 1101 80-81 -1.1335486357521738 1101 82-83 -1.0470551063073472 1101 84-85 -1.2260546554011995 1101 86-87 -1.3424066225878413 1101 88-89 -1.3562782539887976 1101 90-91 -1.4004229898219833 1101 92-93 -1.5580196819373207 1101 94-95 -1.5566341347311479 1101 96-97 -1.6283739123816154 1101 98-99 -1.720325500246613 1101 100 -1.7850067020282339 1102 1 -0.49566280103466553 1102 2 -0.48092386382070273 1102 3 -0.4461308430211517 1102 4 -0.22304828373986396 1102 5 -0.3401750996609607 1102 6 -0.3788933883479757 1102 7 -0.33704918379440585 1102 8 -0.39099713811462067 1102 9 -0.5190284687991493 1102 10-11 -0.5116068398058147 1102 12-13 -0.5639941803475352 1102 14-15 -0.6413801998020787 1102 16-17 -0.6973565480977157 1102 18-19 -0.6534664659792639 1102 20-21 -0.7411931652292125 1102 22-23 -0.713906946918236 1102 24-25 -0.7193531821920729 1102 26-27 -0.7532493591556033 1102 28-29 -0.8467332060617068 1102 30-31 -0.8128031524975441 1102 32-33 -0.8595518218774956 1102 34-35 -0.8385567985325864 1102 36-37 -0.8670547078535193 1102 38-39 -1.0458895190317818 1102 40-41 -0.9556372015995009 1102 42-43 -0.9243567260337429 1102 44-45 -1.069032109892035 1102 46-47 -1.1463909245577284 1102 48-49 -1.049508062367984 1102 50-51 -1.0330784469198946 1102 52-53 -1.013867094420533 1102 54-55 -0.8434249602919621 1102 56-57 -0.8768022855710527 1102 58-59 -0.8688747153530869 1102 60-61 -0.8377457340683705 1102 62-63 -0.9355745613262343 1102 64-65 -0.9549451439722318 1102 66-67 -0.8990565520408111 1102 68-69 -0.8752462678708923 1102 70-71 -0.8963526266617663 1102 72-73 -0.958929575477022 1102 74-75 -0.9866125758681186 1102 76-77 -1.0578956807796267 1102 78-79 -1.043468902831691 1102 80-81 -1.1377122065440695 1102 82-83 -1.1021781333907903 1102 84-85 -1.0757186784888084 1102 86-87 -1.1312978292439375 1102 88-89 -1.2924462497294051 1102 90-91 -1.2900567428698473 1102 92-93 -1.2769517011628686 1102 94-95 -1.3523792108002688 1102 96-97 -1.4304243416983233 1102 98-99 -1.5266095300358806 1102 100 -1.500929299198969 1103 1 -0.48903528699149845 1103 2 -0.4663693129477231 1103 3 -0.5913114315625521 1103 4 -0.43961498209903027 1103 5 -0.41746755619463727 1103 6 -0.37647569428360583 1103 7 -0.3869627141429035 1103 8 -0.37823464794516326 1103 9 -0.52452672571183 1103 10-11 -0.5442229510712551 1103 12-13 -0.6570109408526861 1103 14-15 -0.7780320179471403 1103 16-17 -0.8299700835326647 1103 18-19 -0.7822489915451953 1103 20-21 -0.7758463671495406 1103 22-23 -0.8054817640342762 1103 24-25 -0.8271068884534927 1103 26-27 -0.9620580799394887 1103 28-29 -0.9649405128124684 1103 30-31 -0.8842691782714951 1103 32-33 -0.9573641918632632 1103 34-35 -1.029661477675397 1103 36-37 -0.9522433564619845 1103 38-39 -0.975983109713404 1103 40-41 -1.0359066660745597 1103 42-43 -1.0864870617524858 1103 44-45 -1.111822653790064 1103 46-47 -1.1399540219710715 1103 48-49 -1.192572883305381 1103 50-51 -1.265836620127871 1103 52-53 -1.1306791813910024 1103 54-55 -0.9393848190712433 1103 56-57 -0.8087714395118155 1103 58-59 -0.8486945180770746 1103 60-61 -0.8926356385083167 1103 62-63 -0.9283134581925907 1103 64-65 -0.9191290360977575 1103 66-67 -1.0051279149355423 1103 68-69 -0.9273501996968179 1103 70-71 -0.8796434098837835 1103 72-73 -0.9015360616138466 1103 74-75 -0.9719547961427111 1103 76-77 -0.9353504798452832 1103 78-79 -0.9739218055644194 1103 80-81 -1.0304059448631655 1103 82-83 -0.9538331207855251 1103 84-85 -1.0760891742090202 1103 86-87 -1.083096900119056 1103 88-89 -1.1285162562374502 1103 90-91 -1.238535698660769 1103 92-93 -1.2772417266342444 1103 94-95 -1.3836446087236922 1103 96-97 -1.4000822974931602 1103 98-99 -1.4075727732607959 1103 100 -1.4331762189621742 1104 1 -0.58039201869801 1104 2 -0.5291762900021659 1104 3 -0.5785073758964714 1104 4 -0.46123349941652947 1104 5 -0.4068703659859665 1104 6 -0.418789813645688 1104 7 -0.5448658973850442 1104 8 -0.40814591564269165 1104 9 -0.6542670435004538 1104 10-11 -0.6302015772202125 1104 12-13 -0.5946989687110431 1104 14-15 -0.7781469502342304 1104 16-17 -0.8395257014111692 1104 18-19 -0.8627111600528252 1104 20-21 -0.8822805302545476 1104 22-23 -0.8705099943838022 1104 24-25 -0.9093602088548138 1104 26-27 -0.9671905941155288 1104 28-29 -0.8974419564652223 1104 30-31 -0.9466230723361164 1104 32-33 -0.972863885604383 1104 34-35 -1.1018862074226305 1104 36-37 -1.045504029997808 1104 38-39 -1.0121587835072816 1104 40-41 -1.090756316822585 1104 42-43 -1.0514890482965669 1104 44-45 -1.0389847246259407 1104 46-47 -1.013322767400318 1104 48-49 -1.0101766339785172 1104 50-51 -1.1411820986464107 1104 52-53 -0.9768361436924309 1104 54-55 -0.7359849559869716 1104 56-57 -0.8752696881394897 1104 58-59 -0.838073883726814 1104 60-61 -0.7916807583777441 1104 62-63 -0.8651357082653774 1104 64-65 -0.7877271786790416 1104 66-67 -0.7837692794576228 1104 68-69 -0.961771725849573 1104 70-71 -0.92513225066714 1104 72-73 -0.8307234092756133 1104 74-75 -0.8013521672827082 1104 76-77 -0.7709087182091494 1104 78-79 -0.9221810963097532 1104 80-81 -0.9835369915284495 1104 82-83 -1.0706856120968276 1104 84-85 -0.9885114531061774 1104 86-87 -0.9861454419080538 1104 88-89 -1.155216552953128 1104 90-91 -1.276251138879914 1104 92-93 -1.2352834080295771 1104 94-95 -1.2804189821102305 1104 96-97 -1.384477117287318 1104 98-99 -1.2909266536922637 1104 100 -1.2857440706413392 1105 1 -0.5352645332055133 1105 2 -0.4816718319429647 1105 3 -0.5140348369925256 1105 4 -0.38312589025246524 1105 5 -0.33287901377316587 1105 6 -0.35627892458371235 1105 7 -0.32230189762604056 1105 8 -0.44661904439090705 1105 9 -0.5624289728042484 1105 10-11 -0.6749949214202431 1105 12-13 -0.577250817697859 1105 14-15 -0.7943811113908126 1105 16-17 -0.7246464373533499 1105 18-19 -0.7221942807155202 1105 20-21 -0.6954910006180341 1105 22-23 -0.7481487925150603 1105 24-25 -0.7602274464950867 1105 26-27 -0.7180702820466891 1105 28-29 -0.7946627292837931 1105 30-31 -0.8407994055242867 1105 32-33 -0.8718598998802563 1105 34-35 -0.8068079621139432 1105 36-37 -0.8096859566731567 1105 38-39 -0.8629439253429823 1105 40-41 -0.8737544931688248 1105 42-43 -0.9728521164238799 1105 44-45 -0.9249518480487495 1105 46-47 -1.014959394960023 1105 48-49 -0.9564266177298748 1105 50-51 -0.8931681276308296 1105 52-53 -0.8449178978677381 1105 54-55 -0.7384916705687274 1105 56-57 -0.6314667969536032 1105 58-59 -0.6633759639139001 1105 60-61 -0.7546764328036843 1105 62-63 -0.8209950528957108 1105 64-65 -0.7405104266273028 1105 66-67 -0.6802725912893663 1105 68-69 -0.6925645573091543 1105 70-71 -0.5962057222604855 1105 72-73 -0.6786358404833024 1105 74-75 -0.7113358721798662 1105 76-77 -0.6670842152162137 1105 78-79 -0.7586172655366354 1105 80-81 -0.8098782187613836 1105 82-83 -0.8246890182077706 1105 84-85 -0.7862356941388633 1105 86-87 -0.9350074413318517 1105 88-89 -0.915472980508472 1105 90-91 -0.9052097839956659 1105 92-93 -0.9697466781639683 1105 94-95 -1.1014497821726472 1105 96-97 -1.0985780577612374 1105 98-99 -1.1057486681592152 1105 100 -1.2504839472920253 1106 1 -0.5185275661306825 1106 2 -0.5068426210119128 1106 3 -0.5635403527237806 1106 4 -0.3599138081566764 1106 5 -0.3824773964289463 1106 6 -0.4731430655398725 1106 7 -0.4975368425735738 1106 8 -0.5062197458751143 1106 9 -0.6303799436846234 1106 10-11 -0.5615453264349597 1106 12-13 -0.627396039102905 1106 14-15 -0.7995934605623205 1106 16-17 -0.777133309720341 1106 18-19 -0.863107574006996 1106 20-21 -0.8010213535781787 1106 22-23 -0.7401199893011778 1106 24-25 -0.8033146190824851 1106 26-27 -0.8953981789343288 1106 28-29 -0.8803817940766336 1106 30-31 -0.9351766976837368 1106 32-33 -0.8769831289898491 1106 34-35 -0.8964934426637043 1106 36-37 -0.9205073998134523 1106 38-39 -1.014264783818291 1106 40-41 -1.0024735692591449 1106 42-43 -1.0312154104346618 1106 44-45 -1.0404090949239944 1106 46-47 -0.965329696335381 1106 48-49 -0.9901086612443564 1106 50-51 -0.9673600549381121 1106 52-53 -0.9019304844814258 1106 54-55 -0.8093232635615877 1106 56-57 -0.7439910712996962 1106 58-59 -0.7691098015863176 1106 60-61 -0.8298213555163088 1106 62-63 -0.7552563239826853 1106 64-65 -0.7748306071724329 1106 66-67 -0.794804436248576 1106 68-69 -0.8810342825373354 1106 70-71 -0.8256148387754791 1106 72-73 -0.8472433494723219 1106 74-75 -0.7882249309915537 1106 76-77 -0.795804868077358 1106 78-79 -0.7850735373335631 1106 80-81 -0.7426777338962296 1106 82-83 -0.8994768754794507 1106 84-85 -0.8854532500676981 1106 86-87 -0.9697684268526245 1106 88-89 -0.9172674432307275 1106 90-91 -0.9411047684583771 1106 92-93 -0.9908259338988827 1106 94-95 -1.0997241040453751 1106 96-97 -1.131125874758613 1106 98-99 -1.0499447901645418 1106 100 -1.1243401849689292 1107 1 -0.6283655808465056 1107 2 -0.6282504812342822 1107 3 -0.6442567015682492 1107 4 -0.5969677541368625 1107 5 -0.571668032534653 1107 6 -0.5692360626911253 1107 7 -0.6045323720852878 1107 8 -0.4868975488636025 1107 9 -0.7244073176347214 1107 10-11 -0.6644176159597279 1107 12-13 -0.7873672783329937 1107 14-15 -0.9858217149943727 1107 16-17 -0.9453083999563106 1107 18-19 -0.9990838181256478 1107 20-21 -1.0263971756740347 1107 22-23 -1.056575498082502 1107 24-25 -1.0563715595579026 1107 26-27 -1.0239746894220971 1107 28-29 -1.1235413189462236 1107 30-31 -1.0646352402732404 1107 32-33 -1.1951871525891207 1107 34-35 -1.1338434741939594 1107 36-37 -1.1861478842767 1107 38-39 -1.099720698833309 1107 40-41 -1.1504743545590514 1107 42-43 -1.1186105160058801 1107 44-45 -1.0544217760631582 1107 46-47 -1.0455992520448518 1107 48-49 -0.9983473105924503 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0.7589393476713546 2311 4 0.6075289011767566 2311 5 0.5568108550408652 2311 6 0.6126173519871472 2311 7 0.6409628645432051 2311 8 0.5982868375845953 2311 9 0.7975063566168146 2311 10-11 0.8503456009555279 2311 12-13 0.9027805167649774 2311 14-15 1.1120830641114736 2311 16-17 1.117191014373489 2311 18-19 1.0473242248771868 2311 20-21 1.1039778208119486 2311 22-23 1.1563687119144461 2311 24-25 1.167327487598385 2311 26-27 1.17482565154517 2311 28-29 1.2714197771996751 2311 30-31 1.2119509835884728 2311 32-33 1.2218271727582604 2311 34-35 1.2540524143721612 2311 36-37 1.275291292291918 2311 38-39 1.2464139665580234 2311 40-41 1.3117019193841415 2311 42-43 1.3321710975977012 2311 44-45 1.344299514648604 2311 46-47 1.3775192986674583 2311 48-49 1.3882465834973061 2311 50-51 1.4196133169949618 2311 52-53 1.3334009406445517 2311 54-55 1.19916116534435 2311 56-57 1.1645540663370468 2311 58-59 1.1811304059491121 2311 60-61 1.1168517647058067 2311 62-63 1.1061193303467647 2311 64-65 1.1155538685541444 2311 66-67 1.118292012763007 2311 68-69 1.1488337504322175 2311 70-71 1.0639231326200473 2311 72-73 1.097587994863936 2311 74-75 1.0988384150330575 2311 76-77 1.064425179569227 2311 78-79 1.1238336877991912 2311 80-81 1.1145006995862836 2311 82-83 1.180193552342601 2311 84-85 1.2086288360873958 2311 86-87 1.1693525574344186 2311 88-89 1.256361029177146 2311 90-91 1.2846819504997562 2311 92-93 1.3139372938506355 2311 94-95 1.36692105368547 2311 96-97 1.4331236104406635 2311 98-99 1.4797010924104477 2311 100 1.5170314308588218 2312 1 0.6421770393748076 2312 2 0.6334498640582176 2312 3 0.6662957497457391 2312 4 0.37389716827632924 2312 5 0.5060920631550658 2312 6 0.5982342915441379 2312 7 0.5225080436211442 2312 8 0.5976032679960426 2312 9 0.735812154810219 2312 10-11 0.657466345241815 2312 12-13 0.830376021921758 2312 14-15 1.030617305070166 2312 16-17 1.0206033366625675 2312 18-19 1.030983409490581 2312 20-21 1.0058499473837372 2312 22-23 1.0934993403154536 2312 24-25 1.0582230593133986 2312 26-27 1.1695491551742805 2312 28-29 1.1629247598220047 2312 30-31 1.168936257336135 2312 32-33 1.2240396010577186 2312 34-35 1.1968359341763275 2312 36-37 1.2392936807782036 2312 38-39 1.2943492906432823 2312 40-41 1.368311631655736 2312 42-43 1.3914233970411303 2312 44-45 1.402580747865791 2312 46-47 1.3902312073264866 2312 48-49 1.4126172041572644 2312 50-51 1.4003490359568787 2312 52-53 1.3164274678698504 2312 54-55 1.2880018851234496 2312 56-57 1.1833956573375346 2312 58-59 1.147658057769064 2312 60-61 1.1860460409332347 2312 62-63 1.1966549245555953 2312 64-65 1.1928268559423216 2312 66-67 1.214923181991118 2312 68-69 1.1560476871580931 2312 70-71 1.1521838139151725 2312 72-73 1.1986393285934511 2312 74-75 1.1909933499971643 2312 76-77 1.1921681653221725 2312 78-79 1.220716138376531 2312 80-81 1.238737602422713 2312 82-83 1.2525856775969686 2312 84-85 1.2889309923999228 2312 86-87 1.3240104256282272 2312 88-89 1.2918356028987468 2312 90-91 1.3460724981607335 2312 92-93 1.4177119327394294 2312 94-95 1.4990651947922622 2312 96-97 1.4887486242574717 2312 98-99 1.11616113929119 2312 100 1.3950384367114559 2313 1 0.6027068000027853 2313 2 0.6055886337782574 2313 3 0.5991566397483048 2313 4 0.49000972234834705 2313 5 0.3354535493898183 2313 6 0.49399170662434244 2313 7 0.5220904267947972 2313 8 0.5468251630012162 2313 9 0.6702723485317961 2313 10-11 0.7017331435416096 2313 12-13 0.7725799682837078 2313 14-15 0.949483318770227 2313 16-17 0.9519841209442248 2313 18-19 0.9110696179391056 2313 20-21 0.9739948374144021 2313 22-23 0.9926150671612746 2313 24-25 0.9618239589858177 2313 26-27 0.9738514016589548 2313 28-29 1.045458361911237 2313 30-31 1.0410042610655452 2313 32-33 1.073157751824894 2313 34-35 1.1457558481374193 2313 36-37 1.163758135434648 2313 38-39 1.2601039035815873 2313 40-41 1.2109303329618584 2313 42-43 1.2638987951475045 2313 44-45 1.2234266310301152 2313 46-47 1.334064388093374 2313 48-49 1.2836489260020159 2313 50-51 1.357322998803106 2313 52-53 1.2601788993155054 2313 54-55 1.105161228103114 2313 56-57 1.0862243029293879 2313 58-59 1.0925732338418257 2313 60-61 1.0582902179314075 2313 62-63 1.0516156542039639 2313 64-65 1.0833648377204028 2313 66-67 1.082863142690087 2313 68-69 1.0570830446830541 2313 70-71 1.0790559934576294 2313 72-73 1.1320337638512612 2313 74-75 1.1156247141429745 2313 76-77 0.9225796111393976 2313 78-79 1.053218005462007 2313 80-81 1.1242457758209632 2313 82-83 1.1462358956933798 2313 84-85 1.1795621972652377 2313 86-87 1.2485523710929378 2313 88-89 1.2590391395554228 2313 90-91 1.2476630567912572 2313 92-93 1.283237871604296 2313 94-95 1.399048739978877 2313 96-97 1.3866026001303133 2313 98-99 1.4342516131954675 2313 100 1.6519498418003415 2314 1 0.5404470994690556 2314 2 0.5418775041230717 2314 3 0.5479402062309298 2314 4 0.4344501572453794 2314 5 0.4534210914859429 2314 6 0.44227182235006524 2314 7 0.542408117675528 2314 8 0.545488319108486 2314 9 0.6787414536635055 2314 10-11 0.6867035546392586 2314 12-13 0.7629062239989963 2314 14-15 0.8860117414556399 2314 16-17 0.8538299525981827 2314 18-19 0.8384698927723235 2314 20-21 0.8834784545205068 2314 22-23 0.929465357427631 2314 24-25 0.9260576414787991 2314 26-27 0.9227415953867393 2314 28-29 0.9481799319346536 2314 30-31 0.929256352813006 2314 32-33 0.9522576952205171 2314 34-35 0.9280493794950999 2314 36-37 0.935532755449934 2314 38-39 0.9372713769752536 2314 40-41 1.0294783882993457 2314 42-43 1.1165393193141426 2314 44-45 1.1244598479163344 2314 46-47 1.0932674480709323 2314 48-49 1.105534895486116 2314 50-51 1.0827766252820084 2314 52-53 1.0690067522930065 2314 54-55 0.9893730102253429 2314 56-57 0.9144138228286707 2314 58-59 0.9252067789298835 2314 60-61 0.9301653051771908 2314 62-63 0.8755468741356864 2314 64-65 0.9055237587397116 2314 66-67 0.9357396904278446 2314 68-69 0.9608642822751747 2314 70-71 0.9796040605154985 2314 72-73 0.932477421191372 2314 74-75 0.9561492448438926 2314 76-77 0.9057015213731887 2314 78-79 0.8927026832432006 2314 80-81 0.8978640927063744 2314 82-83 0.9434879489699597 2314 84-85 1.0112122037270233 2314 86-87 1.0475098324146153 2314 88-89 1.0458306546375091 2314 90-91 1.1189081832442476 2314 92-93 1.1984734072804564 2314 94-95 1.2274260147502254 2314 96-97 1.237792512876549 2314 98-99 1.3330058923516752 2314 100 1.4169294146543336 2315 1 0.39100358213660513 2315 2 0.3855222479593685 2315 3 0.4929127119929966 2315 4 0.17946662507154798 2315 5 0.2682274722616427 2315 6 0.3346578023855571 2315 7 0.3069976847924849 2315 8 0.3309247199796701 2315 9 0.4509627755739345 2315 10-11 0.35703069642482177 2315 12-13 0.3988698802360915 2315 14-15 0.6280903571868137 2315 16-17 0.6128704375521323 2315 18-19 0.4681375436728743 2315 20-21 0.608899731126435 2315 22-23 0.4793359717771395 2315 24-25 0.6157426880957431 2315 26-27 0.7113691564977458 2315 28-29 0.7137512669256338 2315 30-31 0.6787015277697535 2315 32-33 0.6902242985851927 2315 34-35 0.7055025780927622 2315 36-37 0.8129094727800847 2315 38-39 0.77563009409117 2315 40-41 0.8301065072826503 2315 42-43 0.8948315151109725 2315 44-45 0.9477768697012792 2315 46-47 0.934168629138945 2315 48-49 0.967088528567146 2315 50-51 0.8861158354966037 2315 52-53 0.9239905510097088 2315 54-55 0.7769565258170914 2315 56-57 0.7409213871354723 2315 58-59 0.6986896900928627 2315 60-61 0.7727069992678608 2315 62-63 0.7282266869498173 2315 64-65 0.7331630650624632 2315 66-67 0.7176970112521062 2315 68-69 0.743566891995485 2315 70-71 0.6820279401266021 2315 72-73 0.3689003405282705 2315 74-75 0.7225051157099358 2315 76-77 0.6669528140939605 2315 78-79 0.762696167826391 2315 80-81 0.5306002503493978 2315 82-83 0.46356913831849766 2315 84-85 0.8103743125835017 2315 86-87 0.9011224626012329 2315 88-89 0.9263334043044971 2315 90-91 0.992577133551034 2315 92-93 1.0177507140215276 2315 94-95 1.0482735411268074 2315 96-97 0.7922138458164092 2315 98-99 0.3121843753065683 2315 100 0.903005667259194 2316 1 0.3643275628812752 2316 2 0.3453255979937495 2316 3 0.35257940813210453 2316 4 0.2353829822775637 2316 5 0.1837479654338594 2316 6 0.22493295711588246 2316 7 0.1812731637196876 2316 8 0.21255237495559953 2316 9 0.24105190024236833 2316 10-11 0.271103257580144 2316 12-13 0.06353193446538086 2316 14-15 0.08703961055158516 2316 16-17 0.25934365692534556 2316 18-19 0.3213076242308901 2316 20-21 0.3443681366672564 2316 22-23 0.3142776507239944 2316 24-25 0.4036087604014682 2316 26-27 0.4535909022487985 2316 28-29 0.47451266784910473 2316 30-31 0.30283680137227265 2316 32-33 0.5271333908339386 2316 34-35 0.5178007360650625 2316 36-37 0.5381028267441295 2316 38-39 0.69658864601179 2316 40-41 0.7662986774368861 2316 42-43 0.7251425820134187 2316 44-45 0.6622094072786453 2316 46-47 0.49178147421213225 2316 48-49 0.37576528463355174 2316 50-51 0.4686993849611909 2316 52-53 0.5864578415097625 2316 54-55 0.3499305753307951 2316 56-57 0.23242003631515473 2316 58-59 0.21663868802979636 2316 60-61 0.2331141070387588 2316 62-63 0.3828445570745842 2316 64-65 0.32742999698112385 2316 66-67 0.24898429880491335 2316 68-69 0.22598758656272366 2316 70-71 0.029612442533519356 2316 72-73 0.009938620319999814 2316 74-75 0.1943043641626332 2316 76-77 0.37171287893333727 2316 78-79 0.3869986778961447 2316 80-81 0.6093809508111327 2316 82-83 0.6434682787055692 2316 84-85 0.7104510293533188 2316 86-87 0.7430468657842546 2316 88-89 0.6737492653910486 2316 90-91 0.5515112479041129 2316 92-93 0.5831697237474351 2316 94-95 0.754563152090558 2316 96-97 0.7105768628121858 2316 98-99 0.6034781173244461 2316 100 0.36625660546530625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 24.0 1 489.0 2 218.0 3 193.0 4 109.0 5 113.0 6 151.0 7 476.0 8 1656.0 9 3494.0 10 5515.0 11 8406.0 12 12575.0 13 15957.0 14 19032.0 15 22656.0 16 27081.0 17 32950.0 18 40144.0 19 49226.0 20 60319.0 21 74479.0 22 82403.0 23 84431.0 24 84285.0 25 87766.0 26 96422.0 27 112287.0 28 131962.0 29 158432.0 30 192104.0 31 240042.0 32 305482.0 33 402619.0 34 569453.0 35 799773.0 36 1204959.0 37 1679577.0 38 1427520.0 39 384948.0 40 303.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.84379274244436 19.773137513769676 13.080866476614425 23.302203267171546 2 15.834990992332335 19.408506201787215 40.74342483071899 24.01307797516146 3 19.483949855309824 25.064885484395532 28.805698471897845 26.6454661883968 4 12.851053184795806 15.899313706636283 38.298642914472694 32.95099019409522 5 13.728787345687005 37.48775604797182 34.42559799069709 14.357858615644082 6 31.42796542197665 36.886813415322464 17.417271187951165 14.267949974749724 7 28.176384463582338 31.8140567554403 21.797134005577224 18.212424775400137 8 26.579334413975698 35.25683633829065 19.313753285118718 18.85007596261494 9 26.132918255613784 15.811768860070119 20.8575841207822 37.1977287635339 10-11 25.920516792863157 25.889477807838563 26.99631910310224 21.193686296196034 12-13 26.03145383279541 23.418520821906462 27.700524733511994 22.84950061178613 14-15 23.295404871707397 24.810050830497538 25.38624739979531 26.508296897999756 16-17 22.395266604221973 27.764560099574165 26.3733215167112 23.46685177949266 18-19 21.962600225904357 27.35799402442708 28.633300022200824 22.046105727467737 20-21 23.157510798796764 26.44127394382167 28.353370437424473 22.047844819957096 22-23 23.329831879740066 26.17838168695223 28.10867751671803 22.383108916589673 24-25 22.8719831926514 26.22928326845933 28.61604223671355 22.282691302175717 26-27 22.790775935650284 26.492704435081265 27.97233785632284 22.744181772945606 28-29 22.267027963438895 26.75414120507963 28.35155734398408 22.627273487497394 30-31 22.92680675750541 26.4969125977226 28.232994343088407 22.34328630168358 32-33 23.03909870058256 26.684308691320442 27.68829329270053 22.58829931539647 34-35 23.06990979850257 26.57871330190699 28.174447967607886 22.17692893198255 36-37 22.85708261278751 26.324190234194838 27.95144033435108 22.867286818666567 38-39 22.93718774043667 26.483103371390605 28.240309745093917 22.339399143078804 40-41 22.83653420667209 25.92070076810744 28.52561837109524 22.71714665412523 42-43 22.749759588525567 26.030172597885592 28.136515106703254 23.08355270688559 44-45 22.85258771864425 26.65743446442366 27.94335259160978 22.54662522532231 46-47 22.86458145821533 26.55694649457346 27.458161624507778 23.120310422703433 48-49 23.31543977879508 26.463870936391466 27.442757120244238 22.77793216456922 50-51 23.042662778237222 26.481378227990714 27.418666492749377 23.057292501022687 52-53 22.76102645550924 26.850977943287084 27.21889476915824 23.169100832045437 54-55 22.668026670845446 26.75535761532047 27.27691300040358 23.299702713430502 56-57 23.236480482682527 26.538634312312958 27.002299285929922 23.222585919074593 58-59 22.68437584933234 26.749313479563302 27.50603202326372 23.060278647840633 60-61 22.72803217456076 27.070431357474313 27.10416777166292 23.09736869630201 62-63 22.909384889906605 26.69040075961135 26.87693580972843 23.523278540753616 64-65 22.47978653430311 26.736312439957725 27.149770367807086 23.63413065793208 66-67 22.79880015662734 26.47383430005731 27.098050806944446 23.6293147363709 68-69 22.5062184592003 26.548222879842832 27.251856864708472 23.693701796248394 70-71 22.597003856475297 26.291409525739912 27.267254961622374 23.844331656162417 72-73 23.003199747066652 26.35792908563014 27.348958969798005 23.289912197505206 74-75 23.089573834680074 26.601357114516038 27.068892806485255 23.24017624431863 76-77 22.941576853586366 26.558714769178504 26.81131591357513 23.68839246366 78-79 22.850827297276204 26.567140669936713 26.87340892377053 23.708623109016553 80-81 22.623733813283796 26.859818930443968 27.181732770529564 23.33471448574267 82-83 23.221039182383976 26.23391237269295 27.78928626325159 22.75576218167149 84-85 23.309221033467082 26.482502003188475 27.05849737940425 23.149779583940187 86-87 22.598732955504136 26.893090990034484 27.291403901716897 23.216772152744483 88-89 23.16977178982539 26.164919341355592 27.199648252275765 23.46566061654325 90-91 23.052057545195577 26.22068653063775 27.22414243064294 23.503113493523735 92-93 23.21026510726041 26.355703634886684 27.17563977404182 23.258391483811085 94-95 22.57407659288359 26.286907626323853 27.592177373993916 23.54683840679865 96-97 22.795072352826438 26.212079418830054 27.31027265921554 23.682575569127973 98-99 22.528526607113008 26.522126867892403 27.14482201675655 23.804524508238035 100 23.119286021630046 26.121471645457078 26.559214154308037 24.200028178604835 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 775.0 1 583.5 2 611.5 3 934.0 4 826.0 5 706.0 6 1076.5 7 1992.0 8 3261.0 9 4005.0 10 3820.5 11 3595.0 12 4014.0 13 4655.5 14 5436.5 15 6480.5 16 7474.0 17 8299.0 18 9037.0 19 9876.5 20 10641.0 21 11434.5 22 12884.0 23 14963.5 24 17598.5 25 20825.0 26 25587.0 27 31782.0 28 39917.0 29 53116.0 30 70964.5 31 89252.5 32 113161.5 33 137230.5 34 156410.5 35 176215.5 36 195447.5 37 224966.0 38 241987.5 39 237303.0 40 230777.0 41 224950.5 42 228597.0 43 242341.0 44 261707.0 45 294028.5 46 326277.0 47 350311.0 48 367838.0 49 370019.5 50 361924.0 51 367692.5 52 368681.0 53 354304.5 54 337761.5 55 303349.5 56 269848.5 57 230678.5 58 193335.5 59 176846.5 60 148166.5 61 112087.5 62 87897.5 63 71278.5 64 53410.5 65 34329.5 66 23241.0 67 16975.5 68 12888.5 69 9652.5 70 7380.0 71 5646.5 72 4333.5 73 3360.0 74 2556.5 75 1938.5 76 1405.5 77 945.5 78 641.5 79 444.5 80 328.0 81 244.0 82 179.0 83 139.0 84 112.0 85 83.5 86 64.0 87 61.0 88 49.5 89 36.5 90 26.5 91 18.0 92 17.5 93 19.0 94 15.5 95 8.0 96 6.0 97 5.5 98 4.0 99 4.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05701879244862638 2 0.13163847021465835 3 0.016068824449696206 4 0.015771913428822296 5 0.018871664486745952 6 0.05084304321444897 7 0.05619931803101438 8 0.048087708940739056 9 0.03912099611034686 10-11 0.0379630431289386 12-13 0.03898441704074486 14-15 0.017375232941541426 16-17 0.01640730301349247 18-19 0.01691205174897812 20-21 0.02056999552614474 22-23 0.02492271109215631 24-25 0.02430513616873857 26-27 0.01893698491133821 28-29 0.017422738704881254 30-31 0.015475002407948378 32-33 0.022256450124708567 34-35 0.02296309835438848 36-37 0.02296309835438848 38-39 0.02083127722451378 40-41 0.024560479646690135 42-43 0.031965440507285545 44-45 0.041288446562726434 46-47 0.04624686061132079 48-49 0.038242139488560076 50-51 0.026561659927380316 52-53 0.03780271117766669 54-55 0.03444167842137399 56-57 0.0364606733633166 58-59 0.041490346056920695 60-61 0.021704195625883087 62-63 0.024251692184981268 64-65 0.02380038743325292 66-67 0.030866869730052062 68-69 0.0374701708342879 70-71 0.03893097305698756 72-73 0.04257110217290174 74-75 0.02578375305269066 76-77 0.01925171059346456 78-79 0.018931046690920734 80-81 0.0230402952198157 82-83 0.019061687540105256 84-85 0.011318248115713588 86-87 0.013628215858112636 88-89 0.01778497015034743 90-91 0.019881161957717258 92-93 0.018651950331299256 94-95 0.022398967414728046 96-97 0.023479723530709092 98-99 0.025985652546884924 100 0.027280184597895184 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8420031.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.98396560308264 #Duplication Level Percentage of deduplicated Percentage of total 1 75.78760993672739 47.734042173946825 2 14.339492030815007 18.0631614566906 3 4.6944265706934845 8.87020804964267 4 1.9799457393084827 4.9881933736230195 5 1.0442716299910002 3.28861842118141 6 0.6072052114693121 2.2946515291917406 7 0.37642107949437187 1.6595944626204129 8 0.24654031457800116 1.2422469354523198 9 0.17045243286436235 0.9662193151641676 >10 0.7189502680845793 7.548993563344171 >50 0.02472350179166836 1.0492574908557528 >100 0.00917207792247189 0.9854690910297953 >500 4.3049437122104117E-4 0.18081893499031326 >1k 2.869695113780155E-4 0.3112123832865445 >5k 0.0 0.0 >10k+ 7.174237726535546E-5 0.8173128189802037 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23509 0.27920324758899345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19135 0.22725569537689352 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11619 0.13799236606136012 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 1.1876440834956546E-5 8 0.0 0.0 0.0 0.0 1.1876440834956546E-5 9 0.0 0.0 0.0 0.0 1.1876440834956546E-5 10-11 0.0 0.0 0.0 5.938220417478273E-6 1.1876440834956546E-5 12-13 0.0 0.0 0.0 1.781466125243482E-5 1.1876440834956546E-5 14-15 0.0 0.0 0.0 2.3752881669913092E-5 1.1876440834956546E-5 16-17 0.0 0.0 0.0 2.3752881669913092E-5 2.3752881669913092E-5 18-19 0.0 0.0 0.0 2.9691102087391367E-5 5.938220417478273E-5 20-21 0.0 0.0 0.0 1.0094974709713065E-4 5.938220417478273E-5 22-23 0.0 0.0 0.0 2.9691102087391367E-4 6.5320424592261E-5 24-25 0.0 0.0 0.0 6.056984825827838E-4 7.125864500973928E-5 26-27 0.0 0.0 0.0 0.0012826556101753071 7.125864500973928E-5 28-29 0.0 0.0 0.0 0.004453665313108705 7.125864500973928E-5 30-31 0.0 0.0 0.0 0.014809921721190812 7.125864500973928E-5 32-33 0.0 0.0 0.0 0.03748798549554034 7.125864500973928E-5 34-35 0.0 0.0 0.0 0.07124083034848684 7.125864500973928E-5 36-37 0.0 0.0 0.0 0.12903159145138537 7.125864500973928E-5 38-39 0.0 1.1876440834956546E-5 0.0 0.2124457736557027 7.125864500973928E-5 40-41 0.0 2.3752881669913092E-5 0.0 0.29949414675551667 7.125864500973928E-5 42-43 0.0 2.3752881669913092E-5 0.0 0.38647719943073844 7.719686542721754E-5 44-45 0.0 2.3752881669913092E-5 0.0 0.49075828818207434 8.313508584469582E-5 46-47 0.0 2.3752881669913092E-5 0.0 0.6185962973295467 8.313508584469582E-5 48-49 0.0 2.3752881669913092E-5 0.0 0.7574912728943635 8.313508584469582E-5 50-51 0.0 2.3752881669913092E-5 0.0 0.9005251880901626 8.313508584469582E-5 52-53 0.0 2.3752881669913092E-5 0.0 1.0364391770054053 1.0094974709713063E-4 54-55 0.0 2.3752881669913092E-5 0.0 1.1846452821848281 1.1876440834956546E-4 56-57 0.0 2.3752881669913092E-5 0.0 1.3617408296952824 1.1876440834956546E-4 58-59 0.0 2.3752881669913092E-5 0.0 1.5635987563466216 1.1876440834956546E-4 60-61 5.938220417478273E-6 3.562932250486964E-5 0.0 1.7662167752113977 1.30640849184522E-4 62-63 1.1876440834956546E-5 4.7505763339826183E-5 0.0 1.9708300361364466 1.30640849184522E-4 64-65 1.1876440834956546E-5 5.3443983757304456E-5 0.0 2.17974256864375 1.30640849184522E-4 66-67 1.1876440834956546E-5 7.125864500973928E-5 0.0 2.4187143729043274 1.30640849184522E-4 68-69 1.1876440834956546E-5 7.125864500973928E-5 0.0 2.6809164954380806 1.30640849184522E-4 70-71 1.1876440834956546E-5 7.125864500973928E-5 0.0 2.9633620113750174 1.30640849184522E-4 72-73 1.1876440834956546E-5 7.125864500973928E-5 0.0 3.247428661485926 1.543937308544351E-4 74-75 1.1876440834956546E-5 7.125864500973928E-5 0.0 3.520770885522868 1.543937308544351E-4 76-77 1.1876440834956546E-5 7.125864500973928E-5 0.0 3.8275512287306306 1.543937308544351E-4 78-79 1.1876440834956546E-5 7.125864500973928E-5 0.0 4.162627192227677 1.543937308544351E-4 80-81 1.1876440834956546E-5 7.125864500973928E-5 0.0 4.524561726672978 1.6033195127191337E-4 82-83 1.1876440834956546E-5 7.125864500973928E-5 0.0 4.885872747974443 1.9002305335930473E-4 84-85 1.1876440834956546E-5 7.125864500973928E-5 0.0 5.23743321135041 1.9002305335930473E-4 86-87 1.1876440834956546E-5 7.125864500973928E-5 0.0 5.610104048310511 1.9002305335930473E-4 88 1.1876440834956546E-5 8.313508584469582E-5 0.0 5.910405793042805 1.9002305335930473E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13295 0.0 49.20568 1 GTATCAA 23460 0.0 40.76472 1 GTGGTAT 4475 0.0 33.75987 1 CAACGCA 27205 0.0 33.453526 5 ATCAACG 27955 0.0 32.58964 3 TATCAAC 29575 0.0 31.922548 2 GTACTAG 6090 0.0 31.91696 1 AACGCAG 30620 0.0 30.428722 6 TCAACGC 31495 0.0 29.34453 4 GCATCGT 6040 0.0 28.79462 8 CTAACGC 2910 0.0 28.108747 3 TAACGCC 3015 0.0 27.441675 4 TCTAACG 3045 0.0 27.202995 2 ACGCAGA 33385 0.0 27.190434 7 CGCAGAG 34095 0.0 26.621845 8 CTGTGCG 8715 0.0 25.835064 9 TAGTACT 8480 0.0 25.777592 4 TAGGCAT 8100 0.0 25.536005 5 GTACATG 31570 0.0 25.46256 1 TACATGG 31670 0.0 25.010792 2 >>END_MODULE