FastQCFastQC Report
Mon 6 Jun 2016
ERR1211265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8420031
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220250.26157860938991795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191270.22716068385021387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116230.13803987182469993No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143550.046.6714861
GTATCAA250650.040.1032831
GTACTAG63950.034.186321
CAACGCA292050.032.8051455
ATCAACG298500.032.090843
GTGGTAT50800.031.3744371
TATCAAC323150.030.903592
AACGCAG327150.029.8025266
TCAACGC332900.029.170094
ACGCAGA358650.026.7788547
GGGCACC72100.026.60177894
CTGTGCG85250.026.1806939
CGCAGAG365350.026.172028
GCATCGT76900.026.0294638
CTAACGC28150.025.8806113
TAGTACT85750.025.6526934
TAACGCC30100.024.516274
TGGTATC64800.024.367922
TCTAACG30150.024.160092
GTACATG325150.024.075231
Produced by FastQC (version 0.11.5)