FastQCFastQC Report
Mon 6 Jun 2016
ERR1211264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5200650
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249180.4791324161402902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188230.361935527289858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122300.23516291232826667No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC57210.11000548008422024No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111850.050.7467841
GTATCAA205150.042.46511
TACCTGG31850.038.273672
GTGGTAT34500.036.6969871
GTACTAG44000.036.4753041
CAACGCA245050.034.336855
ACCTGGG34450.034.112453
ATCAACG247800.034.01273
GCATCGT46500.032.854648
TATCAAC263250.032.7899482
AACGCAG274050.031.5438026
GGGCACC50500.031.17623594
TAGGCAT57750.031.1751775
TCAACGC283400.029.9723244
CTAGGCA62550.029.083444
ACGCAGA298150.028.363417
CGCAGAG298750.028.212318
CTGTGCG64250.028.1678649
TAGTACT66100.027.948164
GTACATG282650.027.5246031
Produced by FastQC (version 0.11.5)