##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211264_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5200650 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46601694019017 31.0 31.0 34.0 30.0 34.0 2 31.477836616576774 31.0 31.0 34.0 28.0 34.0 3 31.535506330939402 33.0 31.0 34.0 28.0 34.0 4 35.142466807033735 37.0 35.0 37.0 32.0 37.0 5 35.20696297578187 37.0 35.0 37.0 32.0 37.0 6 35.271936007999 37.0 35.0 37.0 32.0 37.0 7 35.19113803082307 37.0 35.0 37.0 32.0 37.0 8 35.2006287675579 37.0 35.0 37.0 32.0 37.0 9 36.75404978223876 39.0 37.0 39.0 32.0 39.0 10-11 36.703706075202135 39.0 37.0 39.0 32.0 39.0 12-13 36.586818859181065 39.0 37.0 39.0 32.0 39.0 14-15 37.74264265043793 40.0 37.0 41.0 32.0 41.0 16-17 37.727531270129695 40.0 37.0 41.0 32.0 41.0 18-19 37.764807764414066 40.0 37.0 41.0 32.0 41.0 20-21 37.74107303894706 40.0 37.0 41.0 32.0 41.0 22-23 37.62196821551152 40.0 37.0 41.0 32.0 41.0 24-25 37.51634853335641 40.0 37.0 41.0 32.0 41.0 26-27 37.35605049368829 40.0 36.5 41.0 31.0 41.0 28-29 37.232088200513395 40.0 36.0 41.0 31.0 41.0 30-31 37.06203561093325 39.0 36.0 41.0 30.5 41.0 32-33 36.857355426725505 39.0 35.5 41.0 30.0 41.0 34-35 36.70319700422063 39.0 35.0 41.0 30.0 41.0 36-37 36.59280109217117 39.0 35.0 41.0 30.0 41.0 38-39 36.437904204282155 39.0 35.0 41.0 30.0 41.0 40-41 36.25223847019122 39.0 35.0 40.0 29.0 41.0 42-43 36.029489198465576 38.5 35.0 40.0 28.0 41.0 44-45 35.76635612856085 38.0 35.0 40.0 27.0 41.0 46-47 35.48132002730428 38.0 34.0 40.0 26.0 41.0 48-49 35.22903185178776 38.0 34.0 40.0 26.0 41.0 50-51 34.51236287771721 37.5 33.5 39.5 24.5 40.5 52-53 34.59646188457212 37.5 33.5 39.5 25.0 40.5 54-55 35.05815253862498 38.0 34.0 40.0 26.0 41.0 56-57 34.98888004384067 38.0 34.0 40.0 26.0 41.0 58-59 34.753044138713435 37.0 34.0 40.0 25.5 41.0 60-61 34.51880188053416 37.0 33.0 40.0 25.0 41.0 62-63 34.20566352282887 36.0 33.0 40.0 25.0 41.0 64-65 33.95360474171498 36.0 33.0 39.0 25.0 41.0 66-67 33.78281224462327 35.0 33.0 39.0 24.5 41.0 68-69 33.49909020987761 35.0 33.0 39.0 23.5 41.0 70-71 33.230472825512194 35.0 32.5 38.0 24.0 40.5 72-73 32.92573197581072 35.0 32.0 37.0 23.0 40.0 74-75 32.60970715199062 35.0 32.0 37.0 23.0 39.5 76-77 32.30930152961649 35.0 32.0 36.0 22.0 39.0 78-79 31.998399238556722 35.0 31.0 36.0 21.0 39.0 80-81 31.73532981454241 35.0 31.0 35.5 20.0 37.5 82-83 31.46064280426485 35.0 31.0 35.0 20.0 37.0 84-85 31.22898281945526 34.5 31.0 35.0 20.0 37.0 86-87 31.02102823685501 34.0 31.0 35.0 19.0 36.0 88-89 30.829790603097692 34.0 30.0 35.0 19.0 36.0 90-91 30.61086825685251 34.0 30.0 35.0 18.5 36.0 92-93 30.41264793823849 34.0 30.0 35.0 18.0 35.5 94-95 30.255520656071837 34.0 29.0 35.0 18.0 35.0 96-97 30.08252131945045 34.0 29.0 35.0 17.0 35.0 98-99 29.85726591868324 34.0 29.0 35.0 15.5 35.0 100 28.717002682357013 33.0 26.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5160435924664206 1101 2 -0.42576418428894414 1101 3 -0.4767394567519325 1101 4 -0.37905215595187514 1101 5 -0.3437094133492522 1101 6 -0.30633160426052797 1101 7 -0.32453590256491793 1101 8 -0.3679765587311081 1101 9 -0.4980347648039327 1101 10-11 -0.5202122361132808 1101 12-13 -0.6304690655914342 1101 14-15 -0.7320917852040267 1101 16-17 -0.7649609187865707 1101 18-19 -0.6782841708211365 1101 20-21 -0.8315658858319637 1101 22-23 -0.8884874390614712 1101 24-25 -0.932903089609205 1101 26-27 -0.9805427020032909 1101 28-29 -0.967318181678607 1101 30-31 -1.0240704922558308 1101 32-33 -1.0189991063068078 1101 34-35 -0.9343045725242334 1101 36-37 -0.9607892550966213 1101 38-39 -1.0480292081929505 1101 40-41 -1.0778951570471023 1101 42-43 -1.0486695287776442 1101 44-45 -1.0489062820169792 1101 46-47 -1.299363876078388 1101 48-49 -1.321521867582959 1101 50-51 -1.2809201776593042 1101 52-53 -1.138778357986645 1101 54-55 -0.9766608126688325 1101 56-57 -0.9843382436173869 1101 58-59 -0.9814644781461226 1101 60-61 -0.9379372473991268 1101 62-63 -0.8405487293377902 1101 64-65 -0.8674263344644402 1101 66-67 -1.040280602384236 1101 68-69 -0.905824712647501 1101 70-71 -1.0746645597130637 1101 72-73 -1.10838951934263 1101 74-75 -1.0901910843301792 1101 76-77 -1.155427919154441 1101 78-79 -1.1527221228305535 1101 80-81 -1.1582244414778913 1101 82-83 -1.2314906138773019 1101 84-85 -1.2790522628236225 1101 86-87 -1.3336653193555144 1101 88-89 -1.2715988950178883 1101 90-91 -1.355668313160539 1101 92-93 -1.4770401851241388 1101 94-95 -1.5816125370423642 1101 96-97 -1.6457257232582592 1101 98-99 -1.6916744748385177 1101 100 -1.8256356112113465 1102 1 -0.3984285697748433 1102 2 -0.2897683287502204 1102 3 -0.330002893157495 1102 4 -0.27049251967084587 1102 5 -0.2870433840405937 1102 6 -0.25847745791985943 1102 7 -0.23446495971631975 1102 8 -0.26692549001247556 1102 9 -0.4540758564184273 1102 10-11 -0.49918146479569714 1102 12-13 -0.4982189140146218 1102 14-15 -0.6388095385660932 1102 16-17 -0.6784847159367615 1102 18-19 -0.6807931551176125 1102 20-21 -0.7000842409253565 1102 22-23 -0.7192290541266502 1102 24-25 -0.7172685343461822 1102 26-27 -0.7944233379231491 1102 28-29 -0.8436570420498128 1102 30-31 -0.8332800294195266 1102 32-33 -0.8195140180327343 1102 34-35 -0.8181912224888492 1102 36-37 -0.7634197051204055 1102 38-39 -1.0482671960373935 1102 40-41 -1.0112475294079886 1102 42-43 -0.8357578404489772 1102 44-45 -1.0144840470475955 1102 46-47 -1.0811800930808175 1102 48-49 -1.0382203204372615 1102 50-51 -0.9594986977140181 1102 52-53 -0.810597001852237 1102 54-55 -0.5911881218250556 1102 56-57 -0.7623053902636556 1102 58-59 -0.7064060945186981 1102 60-61 -0.6974079379193228 1102 62-63 -0.6769763132116466 1102 64-65 -0.7894137147759608 1102 66-67 -0.7405777092691821 1102 68-69 -0.8250698192905688 1102 70-71 -0.8830851620654059 1102 72-73 -1.000851902963511 1102 74-75 -0.9755015757332046 1102 76-77 -0.9498127542830019 1102 78-79 -0.9320747060098569 1102 80-81 -1.028375822175093 1102 82-83 -1.0652506910793313 1102 84-85 -1.0535133276037136 1102 86-87 -1.1510054609797358 1102 88-89 -1.2096844676840526 1102 90-91 -1.3174768818669165 1102 92-93 -1.2643387023679402 1102 94-95 -1.37020497274683 1102 96-97 -1.42839072952561 1102 98-99 -1.4703627348675106 1102 100 -1.575295278379727 1103 1 -0.49851421724966016 1103 2 -0.4367925048354877 1103 3 -0.5879568837358349 1103 4 -0.3771939432270841 1103 5 -0.3487186503950639 1103 6 -0.496435056086888 1103 7 -0.466906790624364 1103 8 -0.4740369382977363 1103 9 -0.6347045027690399 1103 10-11 -0.6144023007604744 1103 12-13 -0.7130264495947998 1103 14-15 -0.7845251994317906 1103 16-17 -0.7538969244361695 1103 18-19 -0.7471531767456767 1103 20-21 -0.7772008167034912 1103 22-23 -0.78842817380513 1103 24-25 -0.76591560123682 1103 26-27 -0.7969635214821551 1103 28-29 -0.837348107546326 1103 30-31 -0.9796362119792335 1103 32-33 -0.9005462469896983 1103 34-35 -0.9025519106036342 1103 36-37 -0.899403546093005 1103 38-39 -0.888132252129715 1103 40-41 -0.9393961113985299 1103 42-43 -1.031611100687151 1103 44-45 -1.0156750754200132 1103 46-47 -1.057447553416992 1103 48-49 -1.1155568018939874 1103 50-51 -1.186131022407487 1103 52-53 -1.1309884383276696 1103 54-55 -0.9519343467411758 1103 56-57 -0.8620511338767827 1103 58-59 -0.7885876636712865 1103 60-61 -0.8579515962937805 1103 62-63 -0.7886504221678123 1103 64-65 -0.8148872217103076 1103 66-67 -0.9380314329907762 1103 68-69 -0.8447945069895653 1103 70-71 -0.8502275631122203 1103 72-73 -0.8681990106469755 1103 74-75 -0.8948752223415006 1103 76-77 -0.9661530564644139 1103 78-79 -0.9499965383853848 1103 80-81 -0.9479264196983479 1103 82-83 -0.9395501375372106 1103 84-85 -0.9900486797891013 1103 86-87 -1.1369014051940063 1103 88-89 -1.1232804474501066 1103 90-91 -1.2249193242306653 1103 92-93 -1.2745619361126082 1103 94-95 -1.3108234514624932 1103 96-97 -1.3761130133301585 1103 98-99 -1.4439759533170502 1103 100 -1.400244505718085 1104 1 -0.49568354318899566 1104 2 -0.4642626185666643 1104 3 -0.5027535130466312 1104 4 -0.3077937437709153 1104 5 -0.34322130052099453 1104 6 -0.4256328323992804 1104 7 -0.39656376202906785 1104 8 -0.331705582090116 1104 9 -0.47361410522014324 1104 10-11 -0.5160902607594835 1104 12-13 -0.4909256384787568 1104 14-15 -0.6774232821314996 1104 16-17 -0.8299586255074516 1104 18-19 -0.771015714915464 1104 20-21 -0.8006820428701573 1104 22-23 -0.7461125742918497 1104 24-25 -0.8027405823073437 1104 26-27 -0.7875147785448036 1104 28-29 -0.7483491314098956 1104 30-31 -0.847920430159931 1104 32-33 -0.8837338969998569 1104 34-35 -1.0277427059501179 1104 36-37 -0.987025687271263 1104 38-39 -1.0169637656899084 1104 40-41 -0.9981502306486121 1104 42-43 -1.0174354995103059 1104 44-45 -1.0672748193683717 1104 46-47 -0.9802502770312813 1104 48-49 -0.9992341313765394 1104 50-51 -1.0129626791713733 1104 52-53 -0.9102487269131316 1104 54-55 -0.785638216070808 1104 56-57 -0.7910237716803366 1104 58-59 -0.7445955733174685 1104 60-61 -0.8462395060428562 1104 62-63 -0.914173951735954 1104 64-65 -0.8493686999840762 1104 66-67 -0.8046364806210065 1104 68-69 -0.7833123144123206 1104 70-71 -0.9565085673560176 1104 72-73 -0.8249955696421836 1104 74-75 -0.8853619091138505 1104 76-77 -0.9134023449478335 1104 78-79 -1.0271497389022954 1104 80-81 -0.9001932184782859 1104 82-83 -0.992678816778934 1104 84-85 -0.9862437918266984 1104 86-87 -1.1020926593068268 1104 88-89 -1.0183934068702953 1104 90-91 -0.9907305989065058 1104 92-93 -1.1452364769818288 1104 94-95 -1.14278034286178 1104 96-97 -1.2619779508050257 1104 98-99 -1.2209745024149115 1104 100 -1.131757980157083 1105 1 -0.5470500101498672 1105 2 -0.5723059351458879 1105 3 -0.545316253985419 1105 4 -0.392431571957637 1105 5 -0.2912833712544085 1105 6 -0.4003258811511259 1105 7 -0.3678471003317654 1105 8 -0.40227884882868636 1105 9 -0.6578007988367531 1105 10-11 -0.6671404863663 1105 12-13 -0.612197341088546 1105 14-15 -0.810080591516531 1105 16-17 -0.8044658282165926 1105 18-19 -0.7763011043329797 1105 20-21 -0.7562472028383098 1105 22-23 -0.8055040016368977 1105 24-25 -0.8094808286625081 1105 26-27 -0.7437921140774222 1105 28-29 -0.830277345378029 1105 30-31 -0.8600756881665887 1105 32-33 -0.9152783307637335 1105 34-35 -0.8293144996248998 1105 36-37 -0.7915714349152623 1105 38-39 -0.9011806927811605 1105 40-41 -0.9217600398759629 1105 42-43 -1.0374448282700683 1105 44-45 -0.8555237226966312 1105 46-47 -1.0056009115433184 1105 48-49 -0.9519459769775267 1105 50-51 -0.8100589478607318 1105 52-53 -0.8011294271947236 1105 54-55 -0.6042546966044497 1105 56-57 -0.6118556679389755 1105 58-59 -0.7259960345986798 1105 60-61 -0.6577615741513299 1105 62-63 -0.6611404576318094 1105 64-65 -0.6980647205156032 1105 66-67 -0.777564035892091 1105 68-69 -0.7111628044115932 1105 70-71 -0.620624964520303 1105 72-73 -0.6145932513939911 1105 74-75 -0.7692698907687259 1105 76-77 -0.6807529244659563 1105 78-79 -0.7801799606050679 1105 80-81 -0.7810239349154422 1105 82-83 -0.8605982626441033 1105 84-85 -0.7666486612302101 1105 86-87 -0.7845864013333674 1105 88-89 -0.8328461629701849 1105 90-91 -0.9812572385377187 1105 92-93 -0.9660190456332955 1105 94-95 -1.0019104480934224 1105 96-97 -0.9800239836970057 1105 98-99 -0.9016811838590684 1105 100 -0.9069788826230081 1106 1 -0.5596326101338001 1106 2 -0.5107642462038271 1106 3 -0.6623679256898143 1106 4 -0.5279343866436932 1106 5 -0.4707507102197752 1106 6 -0.5415268053518005 1106 7 -0.5318053448334013 1106 8 -0.5832490251282039 1106 9 -0.5742065241151622 1106 10-11 -0.5958560872119634 1106 12-13 -0.7299047058286661 1106 14-15 -0.9467181239569484 1106 16-17 -0.9247763793490407 1106 18-19 -0.9641207855316054 1106 20-21 -0.8625027197300668 1106 22-23 -0.815982113822578 1106 24-25 -0.8906556132666168 1106 26-27 -0.8742101682883998 1106 28-29 -0.8934617037790247 1106 30-31 -0.9567003473271711 1106 32-33 -0.9463207099658035 1106 34-35 -1.0000260827699776 1106 36-37 -0.9968033330661896 1106 38-39 -1.0144566107106456 1106 40-41 -1.096516047468974 1106 42-43 -1.083258983246715 1106 44-45 -1.192538398702716 1106 46-47 -1.1544059538364237 1106 48-49 -1.064908743209898 1106 50-51 -1.0057075536747462 1106 52-53 -1.106108644928831 1106 54-55 -0.9935308306161019 1106 56-57 -0.9916159471643553 1106 58-59 -0.9946489064050752 1106 60-61 -1.0316790428386966 1106 62-63 -0.977076481801987 1106 64-65 -0.972052274905721 1106 66-67 -1.0021582220041338 1106 68-69 -0.9959751541945039 1106 70-71 -0.918934363948928 1106 72-73 -0.8927824890482796 1106 74-75 -0.9572738190225749 1106 76-77 -0.9331900505714437 1106 78-79 -0.8648279222704005 1106 80-81 -0.9132964887223878 1106 82-83 -0.8985562403917804 1106 84-85 -0.9667522652242262 1106 86-87 -0.9647990783503282 1106 88-89 -0.916012547424323 1106 90-91 -1.0043164966471139 1106 92-93 -1.107314087186591 1106 94-95 -1.1645490227865842 1106 96-97 -1.2184882299644002 1106 98-99 -1.0733462339485804 1106 100 -1.2227568328219718 1107 1 -0.6781865403455853 1107 2 -0.6506230512627944 1107 3 -0.6748656187952982 1107 4 -0.5871567227545427 1107 5 -0.5828642674814404 1107 6 -0.5589902351169371 1107 7 -0.610896389403905 1107 8 -0.5524903016011748 1107 9 -0.7898963527248171 1107 10-11 -0.8607240548660542 1107 12-13 -0.8322253334079548 1107 14-15 -0.9275968926427964 1107 16-17 -0.8644494198316863 1107 18-19 -0.9823506674619225 1107 20-21 -1.0158902372862926 1107 22-23 -0.9965664664494938 1107 24-25 -1.0171817810317876 1107 26-27 -1.10459019287795 1107 28-29 -1.0717847365936777 1107 30-31 -1.0412170778359666 1107 32-33 -1.168102472979072 1107 34-35 -0.9806093523478268 1107 36-37 -1.1925623078606051 1107 38-39 -1.1773041286654333 1107 40-41 -1.2257618164294826 1107 42-43 -1.158657372016279 1107 44-45 -1.1409719631731576 1107 46-47 -1.0552225511735074 1107 48-49 -0.9602541665477844 1107 50-51 -1.0542824127267423 1107 52-53 -1.0246945034271704 1107 54-55 -0.9142887873443897 1107 56-57 -0.8917474943042905 1107 58-59 -0.944684278787669 1107 60-61 -0.9461410403797359 1107 62-63 -0.8771360061788229 1107 64-65 -0.8978125970131785 1107 66-67 -0.9469545178030288 1107 68-69 -0.99663074545699 1107 70-71 -0.8761432251964791 1107 72-73 -0.9643924588182173 1107 74-75 -0.9287757654783633 1107 76-77 -0.93613107031846 1107 78-79 -0.9155833056741045 1107 80-81 -1.0326617526527855 1107 82-83 -1.0962341182047872 1107 84-85 -1.0737959181667804 1107 86-87 -0.9692777678436251 1107 88-89 -0.9933979846599001 1107 90-91 -1.0231924823300709 1107 92-93 -1.0314987895083902 1107 94-95 -1.0303874890504723 1107 96-97 -1.0863535575780787 1107 98-99 -1.0902742042539728 1107 100 -1.0209046456260396 1108 1 -0.6295418348500874 1108 2 -0.5909468305926318 1108 3 -0.5895932105370676 1108 4 -0.4278933639093623 1108 5 -0.45063908174759604 1108 6 -0.44708392120410423 1108 7 -0.4039190494729752 1108 8 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52-53 1.3627943203806794 2311 54-55 1.2860539722823603 2311 56-57 1.1732638272129634 2311 58-59 1.1483992153259734 2311 60-61 1.118713468435267 2311 62-63 1.1510288284688883 2311 64-65 1.1296641578911988 2311 66-67 1.099510622165802 2311 68-69 1.0924356169035363 2311 70-71 1.0572740737497028 2311 72-73 1.1087471031027363 2311 74-75 1.1514156622784526 2311 76-77 1.1648318122671597 2311 78-79 1.1276018468065274 2311 80-81 1.1840502877392893 2311 82-83 1.2547072424256704 2311 84-85 1.2553601028596795 2311 86-87 1.2806207121942386 2311 88-89 1.2934154926513912 2311 90-91 1.3532924633737018 2311 92-93 1.369867958448065 2311 94-95 1.4130318444471008 2311 96-97 1.4333961926011547 2311 98-99 1.4884851484541954 2311 100 1.5967816957523056 2312 1 0.6152583631571815 2312 2 0.6124400037585183 2312 3 0.6821706420819709 2312 4 0.3135437207475249 2312 5 0.46154241546779673 2312 6 0.503233812047128 2312 7 0.4257599593223418 2312 8 0.5713544747355002 2312 9 0.7311656352548326 2312 10-11 0.6709326818129213 2312 12-13 0.8008639101391637 2312 14-15 1.055844563917141 2312 16-17 1.016854485856669 2312 18-19 0.9690543050620946 2312 20-21 0.9908274217039477 2312 22-23 1.0177450410524145 2312 24-25 1.0147129800957586 2312 26-27 1.0600305953458715 2312 28-29 1.1361094834599754 2312 30-31 1.1486746593202213 2312 32-33 1.1235064184854835 2312 34-35 1.1064615908167497 2312 36-37 1.1308887651205097 2312 38-39 1.1632350088984822 2312 40-41 1.1796759933748007 2312 42-43 1.258005111856221 2312 44-45 1.2167379965003633 2312 46-47 1.2536575322946888 2312 48-49 1.332570956721618 2312 50-51 1.3481460168612145 2312 52-53 1.1816562827435817 2312 54-55 1.0640676560697813 2312 56-57 1.0731211373486715 2312 58-59 0.9824517614715234 2312 60-61 0.916358075568688 2312 62-63 0.9581149580382018 2312 64-65 0.9695645266846924 2312 66-67 0.9471013597494746 2312 68-69 0.9589576277713903 2312 70-71 0.9543667850138817 2312 72-73 1.0003505516812297 2312 74-75 1.048842108636812 2312 76-77 1.0134138748634811 2312 78-79 1.0189418951171803 2312 80-81 1.0095997576986946 2312 82-83 1.0158931982678219 2312 84-85 1.0488893429940696 2312 86-87 1.0478471007502606 2312 88-89 1.0408917322582028 2312 90-91 1.1034928976198444 2312 92-93 1.228400322182857 2312 94-95 1.17467244449632 2312 96-97 1.1651625822854044 2312 98-99 0.8523134610465029 2312 100 1.2413586087961086 2313 1 0.5517930628264267 2313 2 0.6081894919529454 2313 3 0.5901627227272144 2313 4 0.45000551104151043 2313 5 0.3047053027715947 2313 6 0.46354215876655047 2313 7 0.5417884039240164 2313 8 0.4979409722904222 2313 9 0.7142902979213304 2313 10-11 0.7376719369805471 2313 12-13 0.7946975793844402 2313 14-15 0.9193907245689488 2313 16-17 0.9271164684571787 2313 18-19 0.9427244954159804 2313 20-21 0.9509333118151275 2313 22-23 0.9715885774480242 2313 24-25 0.9024936900322515 2313 26-27 1.0244613346171008 2313 28-29 1.0892874937817965 2313 30-31 1.003162392513289 2313 32-33 1.104935907596797 2313 34-35 1.1432644713673028 2313 36-37 1.1278640418539752 2313 38-39 1.207583882147084 2313 40-41 1.2704486176248864 2313 42-43 1.2518448440300745 2313 44-45 1.241006849196694 2313 46-47 1.1865315526962874 2313 48-49 1.1790218127366643 2313 50-51 1.2714246417485882 2313 52-53 1.2057820848949632 2313 54-55 1.0549443579344953 2313 56-57 0.9911807640706485 2313 58-59 1.0597772356184691 2313 60-61 1.0323104676453028 2313 62-63 1.0310493295753673 2313 64-65 1.0348821713571397 2313 66-67 1.0126431599324235 2313 68-69 0.9770677815573023 2313 70-71 0.9983533052090579 2313 72-73 0.9971393002898452 2313 74-75 0.9942023714424266 2313 76-77 0.8298742552606555 2313 78-79 1.018362327010962 2313 80-81 1.1551494923405272 2313 82-83 1.106110266799849 2313 84-85 1.1808252235888368 2313 86-87 1.232890220271667 2313 88-89 1.2121444149378995 2313 90-91 1.2619846446843574 2313 92-93 1.397839737159181 2313 94-95 1.3515744204233506 2313 96-97 1.423695030746721 2313 98-99 1.5227146878428748 2313 100 1.6077994056680822 2314 1 0.6011722824882071 2314 2 0.5736544337086968 2314 3 0.602202389575325 2314 4 0.49106443495512764 2314 5 0.5589066236318558 2314 6 0.5433601641732722 2314 7 0.5808727703520304 2314 8 0.5031915559296749 2314 9 0.6921055667951634 2314 10-11 0.7211295559287336 2314 12-13 0.7695714561981575 2314 14-15 0.891822370896584 2314 16-17 0.9374501732013343 2314 18-19 0.8913255791643806 2314 20-21 0.8940706536375487 2314 22-23 0.8760338885767638 2314 24-25 0.9109769824785232 2314 26-27 0.983072432212623 2314 28-29 0.9727870333958251 2314 30-31 1.0346602140075163 2314 32-33 0.9608165524170005 2314 34-35 1.0323485835083233 2314 36-37 0.9584894590712523 2314 38-39 0.9939220186201609 2314 40-41 1.1226634813803216 2314 42-43 1.130685453728816 2314 44-45 1.2038124044338687 2314 46-47 1.3017450678703781 2314 48-49 1.1492234633350478 2314 50-51 1.1627503380211763 2314 52-53 1.1336074531373228 2314 54-55 1.0605559785009575 2314 56-57 0.9234703365858721 2314 58-59 0.9329656779937352 2314 60-61 0.9531642606445061 2314 62-63 0.9592802539455363 2314 64-65 0.9088892913423621 2314 66-67 0.8911039837823083 2314 68-69 1.0145599318696128 2314 70-71 0.9754695505221846 2314 72-73 1.0315626493720202 2314 74-75 1.037876984995151 2314 76-77 1.0003105558246403 2314 78-79 1.035946348557495 2314 80-81 1.100658775169478 2314 82-83 1.0202596250617013 2314 84-85 1.1186468539388947 2314 86-87 1.1314832617194632 2314 88-89 1.1668467672039249 2314 90-91 1.187574665784613 2314 92-93 1.2698616980418436 2314 94-95 1.2390867859191346 2314 96-97 1.3687919385080747 2314 98-99 1.4299909090258005 2314 100 1.538336523308054 2315 1 0.5107687582961162 2315 2 0.5265141521271488 2315 3 0.55726903444274 2315 4 0.29483659597791956 2315 5 0.40755244429660564 2315 6 0.4084266501497069 2315 7 0.480737742716137 2315 8 0.5100688377910458 2315 9 0.6527758412276086 2315 10-11 0.49469492009028215 2315 12-13 0.5763533514963228 2315 14-15 0.7994536968923214 2315 16-17 0.82089125046042 2315 18-19 0.6693282586306282 2315 20-21 0.8216669780782766 2315 22-23 0.6738857517913317 2315 24-25 0.8071104050679949 2315 26-27 0.8820285209323941 2315 28-29 0.8741744140165935 2315 30-31 0.8532370918254486 2315 32-33 0.8802565597111354 2315 34-35 0.9087920598232415 2315 36-37 0.9813550841753553 2315 38-39 0.9704559118239331 2315 40-41 1.0318731492184767 2315 42-43 1.1320642166174792 2315 44-45 1.1438211092837705 2315 46-47 1.1490448443191852 2315 48-49 1.0536421720602078 2315 50-51 0.9739712810802388 2315 52-53 1.0272569171574517 2315 54-55 0.9081767790828223 2315 56-57 0.8083765891737258 2315 58-59 0.8638496969317515 2315 60-61 0.8716895434419953 2315 62-63 0.8478375465936736 2315 64-65 0.7901083574088901 2315 66-67 0.814775267840723 2315 68-69 0.7880139485965287 2315 70-71 0.7718527858702728 2315 72-73 0.5801806418403928 2315 74-75 0.7599446061263819 2315 76-77 0.8865060280652202 2315 78-79 0.8457560327995353 2315 80-81 0.5663522320932657 2315 82-83 0.5997375875011635 2315 84-85 0.8582140646017571 2315 86-87 1.0242472921273347 2315 88-89 1.073800702827139 2315 90-91 1.1057950486663408 2315 92-93 1.1612511482052952 2315 94-95 1.1308843780129152 2315 96-97 0.9940138599594022 2315 98-99 0.44664899508442346 2315 100 0.9337065638242628 2316 1 0.46243378571619687 2316 2 0.37591294468596814 2316 3 0.4332822618292944 2316 4 0.323489159076523 2316 5 0.27642099535827214 2316 6 0.33079491584268084 2316 7 0.2872104816042338 2316 8 0.3688379048758321 2316 9 0.4111924826884419 2316 10-11 0.43625662193655756 2316 12-13 0.272750582929298 2316 14-15 0.26883773077927486 2316 16-17 0.44885940456367734 2316 18-19 0.47949854695660576 2316 20-21 0.5685451477055565 2316 22-23 0.5078119054922823 2316 24-25 0.5363488538321448 2316 26-27 0.5718180569378717 2316 28-29 0.47107207912868176 2316 30-31 0.4258104839238115 2316 32-33 0.6107793410749025 2316 34-35 0.6089461499938054 2316 36-37 0.6563946228823383 2316 38-39 0.7621500273202813 2316 40-41 0.8469685878698527 2316 42-43 0.8332392049762092 2316 44-45 0.761350628362429 2316 46-47 0.5978060953652076 2316 48-49 0.5102336633839997 2316 50-51 0.6205441404110701 2316 52-53 0.7056369102792601 2316 54-55 0.44487592632761164 2316 56-57 0.35449349803945296 2316 58-59 0.39945807849323955 2316 60-61 0.47556297853650165 2316 62-63 0.5845273302557459 2316 64-65 0.5297668280408914 2316 66-67 0.5392280286403022 2316 68-69 0.47142140244456954 2316 70-71 0.21295827949266766 2316 72-73 0.08208773447918816 2316 74-75 0.3769778156661161 2316 76-77 0.5360351573333233 2316 78-79 0.5084445151764214 2316 80-81 0.6882110822056546 2316 82-83 0.6971861871820728 2316 84-85 0.8239213680277366 2316 86-87 0.8807897200622001 2316 88-89 0.8344828950971319 2316 90-91 0.7664720213214089 2316 92-93 0.7588718018597014 2316 94-95 1.0020301285970135 2316 96-97 0.9659443025678769 2316 98-99 0.8637899874417769 2316 100 0.818903339341464 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 19.0 1 320.0 2 120.0 3 91.0 4 95.0 5 77.0 6 91.0 7 385.0 8 1331.0 9 2518.0 10 3828.0 11 5843.0 12 7998.0 13 10095.0 14 11955.0 15 14676.0 16 17333.0 17 22603.0 18 28400.0 19 34608.0 20 43368.0 21 54132.0 22 60156.0 23 59575.0 24 56465.0 25 56504.0 26 60979.0 27 69516.0 28 82050.0 29 97724.0 30 118104.0 31 148497.0 32 187762.0 33 250155.0 34 362166.0 35 498786.0 36 740651.0 37 1013003.0 38 834165.0 39 244349.0 40 157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.17823933563989 20.484090638427308 13.066968751148353 22.270701274784457 2 15.757205621211487 19.916470071105564 41.48145481164467 22.844869496038278 3 19.262167790786254 27.301153338809215 28.55097712919391 24.885701741210625 4 12.407251062062121 16.38285392294689 39.39087924704652 31.819015767944475 5 12.933962585292406 38.58512162347467 33.925197894535614 14.555717896697312 6 29.294527833521194 37.71392919825861 18.55017860018319 14.441364368037002 7 27.443699216359352 32.5231166391511 22.580205757856945 17.45297838663261 8 27.01590802888843 34.37235418473829 19.830929458970036 18.780808327403246 9 27.374441595555037 15.39787212391534 21.455965230083887 35.771721050445734 10-11 26.06176104056338 26.14370667617441 26.84947788992297 20.945054393339245 12-13 26.280190365225113 24.020149143984547 27.534496026619813 22.16516446417052 14-15 23.701490677269106 24.936988953269946 25.967080496341747 25.3944398731192 16-17 21.786250575027424 27.632664019360924 27.079339553117148 23.501745852494505 18-19 21.254403346014097 27.316053748082357 29.858114032262005 21.571428873641544 20-21 22.935478715455563 26.460173348916673 29.216612293591144 21.387735642036624 22-23 23.138596141991783 26.168610672752084 28.980894598615393 21.71189858664074 24-25 22.37970566697103 26.20240961306463 29.799567544799793 21.61831717516455 26-27 22.143514054193563 26.522590524222938 28.950798302503745 22.383097119079757 28-29 21.507826649697748 26.980735917102947 29.437001443832607 22.0744359893667 30-31 22.67560866435921 26.50932750875874 29.172704951096744 21.642358875785305 32-33 22.848206212243063 26.75587275097974 28.42302712869042 21.972893908086775 34-35 22.746890156627863 26.682377278554803 29.202918842694757 21.367813722122577 36-37 22.366189164927302 26.44874008490309 28.85307746048506 22.331993289684547 38-39 22.625104347982454 26.561935798476387 29.15216981778902 21.660790035752136 40-41 22.393632402592115 25.640803967929678 29.75355842463337 22.21200520484484 42-43 22.294314070560294 25.9063833519539 28.949016258028827 22.85028631945698 44-45 22.200125055196164 26.85754143331029 28.84833513176722 22.093998379726322 46-47 22.234894374241023 26.55594858784913 28.220721164829087 22.98843587308076 48-49 22.941465577519953 26.445509481943574 28.129916621221433 22.483108319315043 50-51 22.71824733760622 26.436438609126384 28.001317120265025 22.843996933002373 52-53 22.235440583067252 26.990068628395807 27.749978503732848 23.024512284804093 54-55 22.153737428879143 26.852046878453372 27.903190034625823 23.091025658041662 56-57 23.027963488884392 26.49976840078821 27.387174251092016 23.085093859235386 58-59 22.193364855427124 26.833478106431357 28.209670560808114 22.7634864773334 60-61 22.403560916466517 27.42825776890162 27.5177095722469 22.650471742384962 62-63 22.66794953351826 26.767449609730555 27.244687618555798 23.31991323819539 64-65 21.93926276868478 26.786962900903706 27.677423530313465 23.59635080009805 66-67 22.470449146430493 26.405485018498403 27.52622807458759 23.59783776048351 68-69 21.983737450904147 26.604107253995597 27.74610010345992 23.66605519164034 70-71 22.106986222303483 26.235514961449052 27.889556056489646 23.76794275975782 72-73 22.713748657615966 26.536810298351917 27.792411280017582 22.95702976401454 74-75 22.902610517658648 26.752396553571227 27.38085415225349 22.96413877651663 76-77 22.55532987523817 26.649491584620087 27.175677606702227 23.61950093343952 78-79 22.386102318235572 26.606060734275573 27.402852641962333 23.604984305526518 80-81 22.154083756140157 26.96708069039001 27.757924983363402 23.12091057010644 82-83 23.037327480304928 26.21470453184988 28.57424747636231 22.173720511482884 84-85 23.159270852584168 26.606796050223934 27.371922374481034 22.862010722710867 86-87 22.0536270186095 27.27064331556536 27.754364388913434 22.921365276911708 88-89 22.853093178799 26.133960419889 27.70001004864216 23.312936352669837 90-91 22.881773503229695 26.061666348050082 27.85598094608738 23.200579202632838 92-93 23.08786766264961 26.427015202359478 27.61203437827818 22.873082756712726 94-95 22.015374404188467 26.400545368416985 28.183859318181774 23.400220909212777 96-97 22.313400986388547 26.322414769017254 27.755395796914666 23.608788447679537 98-99 21.87452126190961 26.78933966095462 27.56562985319612 23.77050922393965 100 22.60570135173941 26.410051791128286 26.799288268685302 24.184958588447003 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1850.0 1 1343.0 2 1827.0 3 2515.5 4 1615.5 5 1156.0 6 1753.5 7 3145.5 8 4955.5 9 5706.5 10 4846.0 11 3984.0 12 4138.0 13 4759.5 14 5494.0 15 6427.5 16 7182.5 17 7832.5 18 8541.0 19 8936.5 20 9284.5 21 9680.5 22 10344.0 23 11371.5 24 12419.5 25 13693.5 26 15982.0 27 18771.5 28 22675.0 29 30815.5 30 43036.0 31 55182.5 32 72652.0 33 91841.5 34 106593.0 35 121293.0 36 136231.5 37 159796.5 38 168533.0 39 157358.0 40 145577.0 41 133289.5 42 128826.5 43 134925.5 44 148492.5 45 174486.0 46 197604.5 47 214307.5 48 228011.5 49 228875.5 50 224153.5 51 229467.5 52 230595.5 53 220719.5 54 208806.5 55 183136.5 56 161309.5 57 135319.5 58 108547.5 59 99081.5 60 81767.5 61 59213.0 62 45618.5 63 37445.0 64 27435.5 65 16173.0 66 10414.0 67 7067.0 68 5203.0 69 4111.0 70 3321.5 71 2576.0 72 2105.5 73 1965.5 74 1681.5 75 1261.5 76 883.5 77 615.5 78 428.5 79 286.5 80 215.5 81 162.0 82 124.0 83 96.5 84 67.0 85 48.5 86 42.5 87 34.0 88 22.0 89 17.0 90 18.0 91 16.5 92 11.5 93 9.5 94 5.5 95 5.0 96 5.0 97 1.5 98 0.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05743512830127004 2 0.1311951390691548 3 0.01686327670579639 4 0.016536394489150397 5 0.019939815215405766 6 0.052435753223154795 7 0.05743512830127004 8 0.04966686856450636 9 0.04101410400622999 10-11 0.04002384317344947 12-13 0.04069683597242652 14-15 0.018584215434609135 16-17 0.01726707238518262 18-19 0.017517041139088384 20-21 0.02074740657417823 22-23 0.025669868189553233 24-25 0.024371953505811773 26-27 0.019074538759578128 28-29 0.018218876486593025 30-31 0.01619989808966187 32-33 0.022910597713747323 34-35 0.022920211896589847 36-37 0.022929826079432378 38-39 0.021160816436406986 40-41 0.02429504004307154 42-43 0.03171718919750416 44-45 0.04125445857729322 46-47 0.0473498504994568 48-49 0.04009114245334718 50-51 0.027467720381106207 52-53 0.03986040206512648 54-55 0.037091517406478036 56-57 0.03872592848970802 58-59 0.04394642977320143 60-61 0.023112495553440436 62-63 0.026102506417467048 64-65 0.025352600155749763 66-67 0.03246709545922145 68-69 0.03881245613529078 70-71 0.04113908838318287 72-73 0.045042446617249765 74-75 0.0271696807129878 76-77 0.02043975272321729 78-79 0.020170555603626468 80-81 0.02442002442002442 82-83 0.020430138540374763 84-85 0.012671492986453616 86-87 0.014469345178006595 88-89 0.01812273465816773 90-91 0.020920461865343756 92-93 0.019795602472767827 94-95 0.02363166142693702 96-97 0.024593079711189948 98-99 0.027583090575216563 100 0.02878486343053272 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5200650.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.64492873524169 #Duplication Level Percentage of deduplicated Percentage of total 1 79.65098498081382 53.08334217738143 2 13.077509625587947 17.430993940634917 3 3.8141278042410027 7.625768271022365 4 1.4403062097860397 3.839564188324667 5 0.664189606860484 2.213243450795257 6 0.3483126182909731 1.3927961774152406 7 0.22265063905028862 1.0386975180653755 8 0.15002952288474514 0.7998965488668921 9 0.106518893447623 0.6389049656496274 >10 0.4725144705889662 5.426162996106882 >50 0.0316962022569155 1.4708772608892213 >100 0.01933589779142202 2.395971715430885 >500 0.0011698108731910256 0.5437616954148491 >1k 5.160927841041397E-4 0.6854228987369524 >5k 3.4406185606936656E-5 0.13118240344151352 >10k+ 1.0321855682080997E-4 1.2834137918239739 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24918 0.4791324161402902 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18823 0.361935527289858 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12230 0.23516291232826667 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 5721 0.11000548008422024 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.92283656850586E-5 0.0 7 0.0 0.0 0.0 1.92283656850586E-5 1.92283656850586E-5 8 0.0 0.0 0.0 1.92283656850586E-5 3.84567313701172E-5 9 0.0 0.0 0.0 1.92283656850586E-5 9.614182842529299E-5 10-11 0.0 0.0 0.0 1.92283656850586E-5 1.057560112678223E-4 12-13 0.0 0.0 0.0 1.92283656850586E-5 1.1537019411035159E-4 14-15 0.0 0.0 0.0 2.88425485275879E-5 1.1537019411035159E-4 16-17 0.0 0.0 0.0 5.7685097055175793E-5 1.1537019411035159E-4 18-19 0.0 0.0 0.0 5.7685097055175793E-5 1.3459855979541018E-4 20-21 0.0 0.0 0.0 1.1537019411035159E-4 1.442127426379395E-4 22-23 0.0 0.0 0.0 5.383942391816407E-4 2.0189783969311526E-4 24-25 0.0 0.0 0.0 0.0013267572322690432 2.4996875390576176E-4 26-27 0.0 0.0 0.0 0.0025285300875852058 2.4996875390576176E-4 28-29 0.0 0.0 0.0 0.006653014527030276 2.4996875390576176E-4 30-31 0.0 0.0 0.0 0.01894955438262525 2.4996875390576176E-4 32-33 0.0 0.0 0.0 0.046234605289723396 2.4996875390576176E-4 34-35 0.0 0.0 0.0 0.08556622729851077 2.6919711959082036E-4 36-37 0.0 0.0 0.0 0.14878909367098342 2.6919711959082036E-4 38-39 0.0 0.0 0.0 0.24954572986069049 2.6919711959082036E-4 40-41 0.0 0.0 0.0 0.3644448290117581 2.6919711959082036E-4 42-43 0.0 0.0 0.0 0.4731620085950795 2.6919711959082036E-4 44-45 0.0 0.0 0.0 0.5942237989482084 2.6919711959082036E-4 46-47 0.0 0.0 0.0 0.7347158528260891 2.7881130243334964E-4 48-49 0.0 0.0 0.0 0.8905905992520166 2.88425485275879E-4 50-51 0.0 0.0 0.0 1.0632805514695278 2.88425485275879E-4 52-53 0.0 0.0 0.0 1.23137492428831 2.88425485275879E-4 54-55 0.0 0.0 0.0 1.4081124474825262 2.88425485275879E-4 56-57 0.0 0.0 0.0 1.6150192764365991 2.88425485275879E-4 58-59 0.0 0.0 0.0 1.8568063607433687 2.980396681184083E-4 60-61 0.0 0.0 0.0 2.0977473969599956 3.076538509609376E-4 62-63 1.92283656850586E-5 0.0 0.0 2.346937402055512 3.1726803380346686E-4 64-65 2.88425485275879E-5 0.0 0.0 2.592050993625797 3.268822166459962E-4 66-67 3.84567313701172E-5 0.0 0.0 2.862949823579745 3.268822166459962E-4 68-69 3.84567313701172E-5 0.0 0.0 3.164200628767558 3.268822166459962E-4 70-71 3.84567313701172E-5 0.0 0.0 3.4981204272542854 3.268822166459962E-4 72-73 5.7685097055175793E-5 0.0 0.0 3.821224270043168 3.6533894801611336E-4 74-75 5.7685097055175793E-5 0.0 0.0 4.129108861392326 3.6533894801611336E-4 76-77 5.7685097055175793E-5 0.0 0.0 4.472767827098536 3.6533894801611336E-4 78-79 5.7685097055175793E-5 0.0 0.0 4.8521915529789545 3.6533894801611336E-4 80-81 5.7685097055175793E-5 0.0 0.0 5.25289146548989 3.7495313085864263E-4 82-83 5.7685097055175793E-5 0.0 0.0 5.662542182227222 3.8456731370117197E-4 84-85 5.7685097055175793E-5 0.0 0.0 6.05006105006105 3.8456731370117197E-4 86-87 5.7685097055175793E-5 0.0 0.0 6.4600386490150274 3.8456731370117197E-4 88 5.7685097055175793E-5 0.0 0.0 6.788862930595214 3.8456731370117197E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11185 0.0 50.746784 1 GTATCAA 20515 0.0 42.4651 1 TACCTGG 3185 0.0 38.27367 2 GTGGTAT 3450 0.0 36.696987 1 GTACTAG 4400 0.0 36.475304 1 CAACGCA 24505 0.0 34.33685 5 ACCTGGG 3445 0.0 34.11245 3 ATCAACG 24780 0.0 34.0127 3 GCATCGT 4650 0.0 32.85464 8 TATCAAC 26325 0.0 32.789948 2 AACGCAG 27405 0.0 31.543802 6 GGGCACC 5050 0.0 31.176235 94 TAGGCAT 5775 0.0 31.175177 5 TCAACGC 28340 0.0 29.972324 4 CTAGGCA 6255 0.0 29.08344 4 ACGCAGA 29815 0.0 28.36341 7 CGCAGAG 29875 0.0 28.21231 8 CTGTGCG 6425 0.0 28.167864 9 TAGTACT 6610 0.0 27.94816 4 GTACATG 28265 0.0 27.524603 1 >>END_MODULE