FastQCFastQC Report
Mon 6 Jun 2016
ERR1211264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5200650
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225850.4342726389970485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182510.3509369021180045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120790.23225942910982283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115850.052.3859331
GTATCAA213450.041.6025851
TACCTGG31750.038.925072
GTACTAG43900.037.6931651
GTGGTAT34050.033.824661
GGGCACC52150.032.81159694
CAACGCA262100.032.751765
TAGGCAT55600.032.703885
ATCAACG263350.032.587233
ACCTGGG36500.032.06173
TATCAAC279300.031.6808472
CTAGGCA63300.029.9213624
AACGCAG291900.029.907136
TCAACGC296500.029.2133034
CTGTGCG63150.029.0915289
GTACACG5950.028.4426461
GCATCGT56450.027.7168188
TAGTACT65850.027.6207374
GTACCTG45900.027.038071
ACTGTGC73050.026.8855638
Produced by FastQC (version 0.11.5)