FastQCFastQC Report
Mon 6 Jun 2016
ERR1211263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5744904
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202780.35297369634026954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161370.2808924222232434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98730.17185665765694255No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77600.1350762345201939No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG41300.034.4133452
GTACTAG34650.031.5112841
GTATCAA274300.028.4815521
GTACCTG51500.028.1465281
GCATCGT36750.027.8844498
GGTATCA189500.026.89681
TAGGCAT46750.025.6402035
ACCTGGG53600.025.6083153
GTACATG224000.025.56961
CTGTGCG44850.025.3634459
TAGTACT46150.024.9547674
TACATGG224700.024.9447232
ATCAACG306900.024.8590533
CAACGCA308000.024.785535
TATCAAC319500.024.2453782
CTAGGCA51500.023.8227794
ACATGGG227000.023.6691843
AACGCAG329350.023.5641786
TCAACGC327100.023.5248744
CTAGTAC49950.023.1506163
Produced by FastQC (version 0.11.5)