FastQCFastQC Report
Mon 6 Jun 2016
ERR1211263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5744904
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183110.3187346559664008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156370.27218905659694226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96020.16713943348748733No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76700.1335096287074597No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG44350.033.589392
GTACTAG32450.030.1336331
GTACCTG54200.028.1894721
ACCTGGG52300.028.1280523
GTATCAA273650.026.8685441
GGTATCA196750.026.5461881
GTACATG221150.025.9131161
TACATGG223400.025.368842
ACATGGG220700.024.5115053
TAGTACT47800.024.3848884
CTGTGCG47450.023.9641889
CATGGGG177050.023.3340384
CTAGTAC49550.023.1442533
CAACGCA311450.023.0674155
ATCAACG314000.022.871293
TAGGCAT47200.022.7967665
GCATCGT39400.022.7782658
CCTGGGG52350.022.3552574
TATCAAC327700.022.2418982
CTGGGGG38150.022.046435
Produced by FastQC (version 0.11.5)