##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211263_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5744904 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13735843105472 33.0 31.0 34.0 30.0 34.0 2 32.27783858529229 34.0 31.0 34.0 30.0 34.0 3 32.35454604637432 34.0 31.0 34.0 30.0 34.0 4 35.97236803260768 37.0 35.0 37.0 35.0 37.0 5 35.85704547891488 37.0 35.0 37.0 35.0 37.0 6 35.887842512250856 37.0 35.0 37.0 35.0 37.0 7 35.82739467883188 37.0 35.0 37.0 35.0 37.0 8 35.81600580270793 37.0 35.0 37.0 35.0 37.0 9 37.41624960138586 39.0 37.0 39.0 34.0 39.0 10-11 37.39503453843615 39.0 37.0 39.0 34.0 39.0 12-13 37.297270067524195 39.0 37.0 39.0 34.0 39.0 14-15 38.508240259541324 40.0 38.0 41.0 34.0 41.0 16-17 38.463402347541404 40.0 38.0 41.0 34.0 41.0 18-19 38.48997555050528 40.0 38.0 41.0 34.0 41.0 20-21 38.4519592494496 40.0 38.0 41.0 34.0 41.0 22-23 38.32526235425344 40.0 38.0 41.0 34.0 41.0 24-25 38.254288844513326 40.0 38.0 41.0 34.0 41.0 26-27 38.10962707122695 40.0 37.5 41.0 33.0 41.0 28-29 38.08434005163533 40.0 37.5 41.0 33.0 41.0 30-31 38.09713687121665 40.0 37.5 41.0 33.5 41.0 32-33 37.959008192304 40.0 37.0 41.0 33.0 41.0 34-35 37.80883518680208 40.0 37.0 41.0 33.0 41.0 36-37 37.65737747750006 40.0 37.0 41.0 33.0 41.0 38-39 37.564409083250126 40.0 36.5 41.0 33.0 41.0 40-41 37.46314142063993 40.0 36.0 41.0 32.0 41.0 42-43 37.31345467217555 40.0 36.0 41.0 32.0 41.0 44-45 37.1633945493258 39.0 36.0 41.0 31.0 41.0 46-47 36.91796599212101 39.0 35.5 41.0 31.0 41.0 48-49 36.6768435120935 39.0 35.0 41.0 30.0 41.0 50-51 36.469791140113045 39.0 35.0 40.5 30.0 41.0 52-53 36.26469380515323 39.0 35.0 40.0 29.5 41.0 54-55 36.02208609578158 38.0 35.0 40.0 29.0 41.0 56-57 35.763994228624185 38.0 34.5 40.0 28.5 41.0 58-59 35.4842268034418 38.0 34.0 40.0 28.0 41.0 60-61 35.21227195441386 37.0 34.0 40.0 28.0 41.0 62-63 34.91135021577384 37.0 34.0 40.0 27.0 41.0 64-65 34.53593880768068 36.0 33.5 39.0 26.0 41.0 66-67 34.22368302412016 36.0 33.0 39.0 26.0 40.0 68-69 33.89129679103428 35.0 33.0 38.5 26.0 40.0 70-71 33.56514826009277 35.0 33.0 38.0 26.0 40.0 72-73 33.22499340284885 35.0 32.0 37.0 25.5 39.5 74-75 32.895668665655684 35.0 32.0 37.0 25.0 39.0 76-77 31.91188025074048 34.0 30.5 35.5 24.5 38.0 78-79 32.4176217740105 35.0 32.0 36.0 25.0 38.0 80-81 32.35165844372682 35.0 32.0 35.5 25.0 37.0 82-83 32.10687950573239 34.5 32.0 35.0 25.0 37.0 84-85 31.857178292274334 34.0 31.0 35.0 24.5 36.5 86-87 31.632601345470697 34.0 31.0 35.0 24.0 36.0 88-89 31.42122601178366 34.0 31.0 35.0 24.0 36.0 90-91 31.209061718002598 34.0 31.0 35.0 23.0 35.0 92-93 31.034780476749482 34.0 31.0 35.0 23.0 35.0 94-95 30.851524063761552 34.0 30.0 35.0 20.5 35.0 96-97 30.683656332638456 34.0 30.0 35.0 20.0 35.0 98-99 30.512336759674312 34.0 30.0 35.0 20.0 35.0 100 28.72122806577795 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4163042338643663 1101 2 -0.38577804407839267 1101 3 -0.44967486951454916 1101 4 -0.25193700989190404 1101 5 -0.27644912103873764 1101 6 -0.4332219086423592 1101 7 -0.3491906984353932 1101 8 -0.3876344497864963 1101 9 -0.49496451448427337 1101 10-11 -0.500788366946864 1101 12-13 -0.5165532332525871 1101 14-15 -0.7597110559279017 1101 16-17 -0.7114477143612703 1101 18-19 -0.7946230778716128 1101 20-21 -0.7483537982665709 1101 22-23 -0.7405810385523921 1101 24-25 -0.7819188738822049 1101 26-27 -0.8537583579822225 1101 28-29 -0.8823339775299672 1101 30-31 -0.7582080129089093 1101 32-33 -0.6778685042246906 1101 34-35 -0.744573196434672 1101 36-37 -0.665499334651507 1101 38-39 -0.7494348112046154 1101 40-41 -0.8113518768838404 1101 42-43 -0.8130741969077562 1101 44-45 -0.8646371908485477 1101 46-47 -0.8599927688951112 1101 48-49 -0.8433780870706684 1101 50-51 -0.9992525670868844 1101 52-53 -0.9991416586686626 1101 54-55 -0.9170457327698074 1101 56-57 -0.940051823878882 1101 58-59 -0.9380419884855087 1101 60-61 -0.9272485734436557 1101 62-63 -1.061713020653002 1101 64-65 -0.9313873867673479 1101 66-67 -0.9752194051443936 1101 68-69 -1.0451234197104569 1101 70-71 -1.0261037448604355 1101 72-73 -1.1190435770965905 1101 74-75 -1.1234432789329105 1101 76-77 -1.050679958233946 1101 78-79 -1.0703549095889322 1101 80-81 -1.021664937315009 1101 82-83 -1.0191131736426087 1101 84-85 -0.862055709095447 1101 86-87 -1.006189498509002 1101 88-89 -1.0222928110822558 1101 90-91 -1.031251941744575 1101 92-93 -1.0963126312557634 1101 94-95 -1.0530012343526707 1101 96-97 -1.189239044091348 1101 98-99 -1.3501720078469894 1101 100 -1.1746783262775438 1102 1 -0.417404276283019 1102 2 -0.37383511684168624 1102 3 -0.34395199594982273 1102 4 -0.24900323546291503 1102 5 -0.3580135529276518 1102 6 -0.36775444345553154 1102 7 -0.38413867363724563 1102 8 -0.38116367419573294 1102 9 -0.5391362866946636 1102 10-11 -0.5104369881949609 1102 12-13 -0.46548832105627724 1102 14-15 -0.6147866077712152 1102 16-17 -0.7065666906578443 1102 18-19 -0.6974944269158811 1102 20-21 -0.7782599123829428 1102 22-23 -0.6844894616427126 1102 24-25 -0.7145245305768455 1102 26-27 -0.8001194645988008 1102 28-29 -0.8073894971292788 1102 30-31 -0.7636419731928186 1102 32-33 -0.7393551212134355 1102 34-35 -0.7395788376778398 1102 36-37 -0.8443255588522902 1102 38-39 -0.8906135358463487 1102 40-41 -0.8312462238334106 1102 42-43 -0.731986100222386 1102 44-45 -0.711802593701826 1102 46-47 -0.8065715261890034 1102 48-49 -0.8621239964405234 1102 50-51 -0.9074565490465716 1102 52-53 -0.9848164723400004 1102 54-55 -0.9552444743498469 1102 56-57 -0.9201002992126561 1102 58-59 -1.0200638238865736 1102 60-61 -1.0031656216595266 1102 62-63 -1.0791453548886452 1102 64-65 -0.9523735766542103 1102 66-67 -1.0303587853947889 1102 68-69 -1.1999636282178017 1102 70-71 -1.1246706577301993 1102 72-73 -0.9482367554196358 1102 74-75 -1.143759808066946 1102 76-77 -0.9045682980612675 1102 78-79 -0.8069408314074238 1102 80-81 -0.8231068434429751 1102 82-83 -0.6894062704810366 1102 84-85 -0.8514912240010091 1102 86-87 -0.8253986239122355 1102 88-89 -0.9114556294590024 1102 90-91 -0.9159820111741404 1102 92-93 -0.9398227599839757 1102 94-95 -0.9962129930648942 1102 96-97 -1.0872445658043013 1102 98-99 -1.032088853444563 1102 100 -1.2676346073718001 1103 1 -0.36612147329898903 1103 2 -0.3537607640918665 1103 3 -0.3476794055664101 1103 4 -0.19790325874099324 1103 5 -0.254984864883653 1103 6 -0.32706134304300605 1103 7 -0.39296049657333043 1103 8 -0.353194027293263 1103 9 -0.5554044694118261 1103 10-11 -0.5671044147537927 1103 12-13 -0.5484285815724022 1103 14-15 -0.6595504548733331 1103 16-17 -0.6685701135271813 1103 18-19 -0.6863228453075436 1103 20-21 -0.7006970557368177 1103 22-23 -0.7223120730580774 1103 24-25 -0.7910124169331496 1103 26-27 -0.7244721269050629 1103 28-29 -0.8684209070749063 1103 30-31 -0.7805474589433032 1103 32-33 -0.7224452963474306 1103 34-35 -0.8794323228845968 1103 36-37 -0.8215463327579684 1103 38-39 -0.8269613001298453 1103 40-41 -0.9159854305935085 1103 42-43 -0.7102764478708607 1103 44-45 -0.8043453168519576 1103 46-47 -0.8393460324924646 1103 48-49 -0.9013882516382736 1103 50-51 -0.9542421687364353 1103 52-53 -0.9921181463197115 1103 54-55 -0.9234736528670382 1103 56-57 -0.9804727105473887 1103 58-59 -0.9935236420725246 1103 60-61 -0.9866267000121383 1103 62-63 -0.9685220843361151 1103 64-65 -0.9494475542710887 1103 66-67 -1.0997952444081633 1103 68-69 -1.0230640144938832 1103 70-71 -1.0612195892430591 1103 72-73 -1.090208458260669 1103 74-75 -1.0071423549921121 1103 76-77 -0.902892086798996 1103 78-79 -0.8286644491475386 1103 80-81 -0.791705721894651 1103 82-83 -0.8156269840037353 1103 84-85 -0.7958802079858103 1103 86-87 -0.8126815149912474 1103 88-89 -0.9026416461013724 1103 90-91 -0.9166350298275248 1103 92-93 -0.9813469286386152 1103 94-95 -0.9426380067312827 1103 96-97 -1.0837648139404514 1103 98-99 -1.1064850308806946 1103 100 -1.0950356040702793 1104 1 -0.3570046074248552 1104 2 -0.3294914941352829 1104 3 -0.3793081444807349 1104 4 -0.2574359792043168 1104 5 -0.3028104528836266 1104 6 -0.38018523291724193 1104 7 -0.35100056328886353 1104 8 -0.37637866475372306 1104 9 -0.5106032588871727 1104 10-11 -0.5424860831106955 1104 12-13 -0.5689958742398531 1104 14-15 -0.7526147490595392 1104 16-17 -0.6669463346520956 1104 18-19 -0.7256649502596559 1104 20-21 -0.7493152721900955 1104 22-23 -0.7649705595283223 1104 24-25 -0.7881702263408528 1104 26-27 -0.83010205081316 1104 28-29 -0.9039089347479745 1104 30-31 -0.8096652317223558 1104 32-33 -0.7821208758263651 1104 34-35 -0.7801596520345697 1104 36-37 -0.7729429448448641 1104 38-39 -0.721428298998795 1104 40-41 -0.828884519798784 1104 42-43 -0.6868789456417517 1104 44-45 -0.8455333601487212 1104 46-47 -0.9032820234870229 1104 48-49 -0.81840709613396 1104 50-51 -0.8700893436523955 1104 52-53 -0.7659500817945073 1104 54-55 -0.8270225953234913 1104 56-57 -0.8172550226348605 1104 58-59 -0.9224391664580622 1104 60-61 -0.9081893638110898 1104 62-63 -0.9633488579745801 1104 64-65 -0.8696684500911331 1104 66-67 -0.8561864526741516 1104 68-69 -0.9467748824599198 1104 70-71 -0.9813261011427485 1104 72-73 -0.9874086901876424 1104 74-75 -0.9196720738580311 1104 76-77 -0.7905337665158534 1104 78-79 -0.7058652247876402 1104 80-81 -0.7653547612953702 1104 82-83 -0.7477136131950246 1104 84-85 -0.6586325053887307 1104 86-87 -0.7283696054599567 1104 88-89 -0.7720542145571123 1104 90-91 -0.8507614429175554 1104 92-93 -0.908737745989896 1104 94-95 -0.886504782828279 1104 96-97 -0.8619714911246383 1104 98-99 -0.9158737640699108 1104 100 -1.0974056990739456 1105 1 -0.4098662398398112 1105 2 -0.4014453294648064 1105 3 -0.43418746503837014 1105 4 -0.2376976326261655 1105 5 -0.27732922506306323 1105 6 -0.3838018619019934 1105 7 -0.39151112909080155 1105 8 -0.4462095635768861 1105 9 -0.5594764703334931 1105 10-11 -0.5785088864949941 1105 12-13 -0.6015687453617389 1105 14-15 -0.8410826837122798 1105 16-17 -0.7763816900161373 1105 18-19 -0.7969469715929876 1105 20-21 -0.9218847248793551 1105 22-23 -0.9003104892814591 1105 24-25 -0.812222686232559 1105 26-27 -0.930648679154011 1105 28-29 -0.9867408943976343 1105 30-31 -0.8839315896604916 1105 32-33 -0.9303141015539396 1105 34-35 -0.7874816865709988 1105 36-37 -0.7407394056333274 1105 38-39 -0.8895898881806303 1105 40-41 -0.9749327753178463 1105 42-43 -0.8159018343798863 1105 44-45 -0.974179536755905 1105 46-47 -0.8553678663627693 1105 48-49 -0.9997343571501105 1105 50-51 -1.0161887793970834 1105 52-53 -1.0644409657246001 1105 54-55 -1.0400717024558475 1105 56-57 -1.0075248114129494 1105 58-59 -1.109337638367336 1105 60-61 -1.0361855996846572 1105 62-63 -1.0938513205082003 1105 64-65 -1.1329482479458264 1105 66-67 -1.0355323383722919 1105 68-69 -1.028719129920212 1105 70-71 -1.1219306952338215 1105 72-73 -1.0876624093375185 1105 74-75 -1.0420199808809443 1105 76-77 -1.1785114833836339 1105 78-79 -1.0126466100315206 1105 80-81 -0.8297848895379332 1105 82-83 -0.8887546029403701 1105 84-85 -0.9459085023228369 1105 86-87 -0.964551479338617 1105 88-89 -0.8433753831476629 1105 90-91 -0.865094312687468 1105 92-93 -0.9320112717266511 1105 94-95 -1.011050712080415 1105 96-97 -1.09762760450365 1105 98-99 -1.136838211301015 1105 100 -1.2158398430122048 1106 1 -0.49323996868034214 1106 2 -0.45770470622004567 1106 3 -0.5117705096493737 1106 4 -0.3603781565214277 1106 5 -0.4139431137998031 1106 6 -0.4097906244817082 1106 7 -0.4402292293595593 1106 8 -0.4143859505320009 1106 9 -0.6204879752770438 1106 10-11 -0.5897847898659094 1106 12-13 -0.6475844060449631 1106 14-15 -0.8308012423139814 1106 16-17 -0.7889402905642129 1106 18-19 -0.7376443463504927 1106 20-21 -0.7854847753674221 1106 22-23 -0.7426833412201432 1106 24-25 -0.7696709718645565 1106 26-27 -0.7637843469327024 1106 28-29 -0.761605312353403 1106 30-31 -0.760812813301925 1106 32-33 -0.8294763792009405 1106 34-35 -0.8572991234152667 1106 36-37 -0.8672005448667335 1106 38-39 -0.9203950256664442 1106 40-41 -0.935321507471933 1106 42-43 -0.8675641879827083 1106 44-45 -0.8755464997909996 1106 46-47 -0.8979664712726247 1106 48-49 -0.8631562091457354 1106 50-51 -1.0814463591205765 1106 52-53 -1.0565656640392547 1106 54-55 -1.0233108686117518 1106 56-57 -1.1189815906739256 1106 58-59 -1.0422707224068049 1106 60-61 -1.1415781094230582 1106 62-63 -1.0830133268070838 1106 64-65 -0.9531045812649452 1106 66-67 -1.0229116200344492 1106 68-69 -0.9559472641875928 1106 70-71 -0.9990173784714855 1106 72-73 -1.1082967194735431 1106 74-75 -1.1322587809333946 1106 76-77 -1.0889391640955246 1106 78-79 -0.9454777941939696 1106 80-81 -0.8161075914377101 1106 82-83 -0.8097314106871281 1106 84-85 -0.94496511658107 1106 86-87 -1.0870302782825831 1106 88-89 -0.9894299851719914 1106 90-91 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1107 48-49 -1.00404686269993 1107 50-51 -0.9784245538963177 1107 52-53 -0.9684563778820063 1107 54-55 -0.9837699149757526 1107 56-57 -1.0150742688585694 1107 58-59 -1.0635562600305946 1107 60-61 -1.0953684220509103 1107 62-63 -0.9854981855698455 1107 64-65 -0.9892596722899683 1107 66-67 -1.0082093227042677 1107 68-69 -1.0102320095125918 1107 70-71 -1.0513234611801607 1107 72-73 -1.0039622282099714 1107 74-75 -0.9751082940131184 1107 76-77 -0.9861289818527901 1107 78-79 -0.8986868179716581 1107 80-81 -0.8313197316536822 1107 82-83 -0.8730210449198736 1107 84-85 -0.9172047774882586 1107 86-87 -0.9158358163099294 1107 88-89 -0.881740090854219 1107 90-91 -0.9277914978202624 1107 92-93 -0.9101513910901069 1107 94-95 -0.9745439178246542 1107 96-97 -0.9638688201519265 1107 98-99 -1.0227473031472591 1107 100 -0.9874628011149866 1108 1 -0.4169911986779269 1108 2 -0.4131674190367214 1108 3 -0.4175948862503418 1108 4 -0.2709198236007211 1108 5 -0.30695172753980415 1108 6 -0.38606743451634173 1108 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2311 44-45 1.1101787337835347 2311 46-47 0.7634115785875508 2311 48-49 1.0845172407401904 2311 50-51 1.211190020678572 2311 52-53 1.0932344063100174 2311 54-55 1.1668545416551623 2311 56-57 1.264118739180681 2311 58-59 1.2350203682397733 2311 60-61 1.2438982868068464 2311 62-63 1.2637121002307268 2311 64-65 1.2980730381964136 2311 66-67 1.344988796008856 2311 68-69 1.3038531985347674 2311 70-71 1.3274140927625382 2311 72-73 1.3451250239645915 2311 74-75 1.3067610916520565 2311 76-77 1.307166888681767 2311 78-79 1.0773345188070422 2311 80-81 0.9979178874700381 2311 82-83 1.146886158768325 2311 84-85 1.1815499173383586 2311 86-87 1.1433484551181863 2311 88-89 1.1495809228554812 2311 90-91 1.2832202594562325 2311 92-93 1.328136926275377 2311 94-95 1.2867111417802555 2311 96-97 1.3721741383294876 2311 98-99 1.4104914533729627 2311 100 1.5789527721612657 2312 1 0.3134377393362797 2312 2 0.37597248966802965 2312 3 0.4745218925525805 2312 4 0.13545687393636996 2312 5 0.33384259166275143 2312 6 0.39067250442543866 2312 7 0.3194593501962686 2312 8 0.4245466437004666 2312 9 0.6836992780641395 2312 10-11 0.6524687953850119 2312 12-13 0.7182885400131269 2312 14-15 0.903257415475565 2312 16-17 0.9150361092804999 2312 18-19 0.8496220325647528 2312 20-21 0.8443005890131374 2312 22-23 0.9460908818788241 2312 24-25 0.9582091136598478 2312 26-27 0.970619367086222 2312 28-29 1.0209988149746607 2312 30-31 1.0025945683033797 2312 32-33 0.9047799511084378 2312 34-35 0.9771574296646861 2312 36-37 1.0808532908143178 2312 38-39 0.8641749714423383 2312 40-41 1.0218004076023632 2312 42-43 1.064836598977486 2312 44-45 0.8368702189339245 2312 46-47 0.8975475421586623 2312 48-49 1.10512424664811 2312 50-51 0.9139916411098454 2312 52-53 1.1229517948144974 2312 54-55 0.999894138238254 2312 56-57 0.9750560972013318 2312 58-59 1.1875355607706837 2312 60-61 1.195802103922837 2312 62-63 1.230070847027676 2312 64-65 1.1689764278434467 2312 66-67 1.2002927295923413 2312 68-69 1.2393162517060574 2312 70-71 1.1369628483222058 2312 72-73 1.1510420712840599 2312 74-75 1.2049458498950614 2312 76-77 1.1958003628981224 2312 78-79 1.0531617818442598 2312 80-81 0.9830769715012693 2312 82-83 1.0179459973099085 2312 84-85 1.0232267966411968 2312 86-87 1.070121088255803 2312 88-89 1.1041659026553923 2312 90-91 1.1428917246791492 2312 92-93 1.1595784527109032 2312 94-95 1.2175403841330272 2312 96-97 0.8605072385697703 2312 98-99 0.6120254821927453 2312 100 0.8039298381755877 2313 1 0.015779355871316625 2313 2 0.2228065774467396 2313 3 0.24866975385487677 2313 4 0.1787372800315552 2313 5 0.3163470040603329 2313 6 0.26928336973941924 2313 7 0.28283275739750735 2313 8 0.4133707446439132 2313 9 0.6097341098103541 2313 10-11 0.37678621471629015 2313 12-13 0.4002335276229374 2313 14-15 0.49602675303432164 2313 16-17 0.7399104577458502 2313 18-19 0.6848328339090344 2313 20-21 0.6744903353517984 2313 22-23 0.6093446828843128 2313 24-25 0.5369088427640634 2313 26-27 0.7586256429554794 2313 28-29 0.5754580916554062 2313 30-31 0.7947135156810106 2313 32-33 0.919648007253457 2313 34-35 0.9081975113274723 2313 36-37 0.9638004997927823 2313 38-39 0.9616280631653851 2313 40-41 0.9430220729428171 2313 42-43 1.0651889943248634 2313 44-45 0.9358149105535958 2313 46-47 0.9601749060907281 2313 48-49 1.030089015726496 2313 50-51 0.9876065780645575 2313 52-53 1.054341795550485 2313 54-55 0.9697753877567266 2313 56-57 0.7189912388005055 2313 58-59 0.7745425652674669 2313 60-61 0.680825479305021 2313 62-63 1.047265957062315 2313 64-65 1.0750302894336699 2313 66-67 1.0787056652351836 2313 68-69 1.2254813509773044 2313 70-71 1.0982290431014476 2313 72-73 1.1574253462440751 2313 74-75 1.2514514416020646 2313 76-77 1.1438035686180648 2313 78-79 1.0199923907807502 2313 80-81 0.8618443050436468 2313 82-83 1.0042258772721908 2313 84-85 1.0061016811876087 2313 86-87 0.9741707827287911 2313 88-89 1.0354529980499656 2313 90-91 0.9358077201140169 2313 92-93 1.1331792705962656 2313 94-95 1.3034644170370662 2313 96-97 1.3197759306392207 2313 98-99 1.30710581231153 2313 100 1.389484034148957 2314 1 -0.3998489190527508 2314 2 0.03251037940664503 2314 3 0.3082782020180517 2314 4 0.2390223653986041 2314 5 0.34127225750989254 2314 6 0.40361617464338906 2314 7 0.501172298859835 2314 8 0.5095604277461874 2314 9 0.5001216309713996 2314 10-11 0.6106701738925366 2314 12-13 0.45676964730802183 2314 14-15 0.7957496877960111 2314 16-17 0.8090881382618278 2314 18-19 0.8475854839950756 2314 20-21 0.9041691712726418 2314 22-23 0.9258388618889555 2314 24-25 0.9019913816767371 2314 26-27 0.7933380371668974 2314 28-29 0.820094825752065 2314 30-31 0.8523833462060821 2314 32-33 0.8957227923618092 2314 34-35 0.9206125132029044 2314 36-37 0.8796728013219735 2314 38-39 0.6908815857594917 2314 40-41 0.6560643570634923 2314 42-43 0.8468712926295154 2314 44-45 0.919490434241645 2314 46-47 0.9204595500821284 2314 48-49 0.8970729260016626 2314 50-51 0.8563505258050128 2314 52-53 0.8606103937047749 2314 54-55 0.9131075007835392 2314 56-57 0.9111903904931538 2314 58-59 1.0155132269445346 2314 60-61 1.0577043863123379 2314 62-63 1.013142153939846 2314 64-65 0.9960221737845885 2314 66-67 0.7065045802338972 2314 68-69 0.6839946628670006 2314 70-71 0.8407688785033116 2314 72-73 0.9879312139586247 2314 74-75 1.0005731561016376 2314 76-77 0.8728669873872867 2314 78-79 0.7679901796552784 2314 80-81 0.8086926205542824 2314 82-83 0.8217018880890592 2314 84-85 0.8846810503568676 2314 86-87 0.9715603996746793 2314 88-89 0.9391990552329226 2314 90-91 0.9767430321815915 2314 92-93 0.9104458969672748 2314 94-95 0.9175758566445538 2314 96-97 0.9838260585881926 2314 98-99 1.117419264392126 2314 100 1.0968651758495298 2315 1 0.41437947898987204 2315 2 0.4439915645018928 2315 3 0.4767603621218086 2315 4 0.3170115483230731 2315 5 0.3598201272082875 2315 6 0.4430114624088688 2315 7 0.41832646978066634 2315 8 0.28303507982452203 2315 9 0.5304722201156906 2315 10-11 0.5540414957649276 2315 12-13 0.3832591222751418 2315 14-15 0.6100673370596894 2315 16-17 0.7803236626048502 2315 18-19 0.7503841493372079 2315 20-21 0.5589664315907115 2315 22-23 0.8712380979789884 2315 24-25 0.903307873117825 2315 26-27 0.8438000451785257 2315 28-29 0.7537363273111524 2315 30-31 0.8115108860572491 2315 32-33 0.7816765472943388 2315 34-35 0.7941232681906243 2315 36-37 0.8117832348328307 2315 38-39 0.8193641080421159 2315 40-41 0.8762242676570509 2315 42-43 0.913530313297855 2315 44-45 0.9170976104422408 2315 46-47 0.8789141845919062 2315 48-49 0.768274646983663 2315 50-51 0.8601161799805297 2315 52-53 0.9488344352530049 2315 54-55 0.7277558538469933 2315 56-57 0.689970962988717 2315 58-59 0.6592896142620646 2315 60-61 0.7773655823534327 2315 62-63 0.9062242213717937 2315 64-65 0.9920146358650399 2315 66-67 1.009630902474207 2315 68-69 1.0294310941518958 2315 70-71 0.8956372523546818 2315 72-73 1.00026441046883 2315 74-75 0.3928903362574587 2315 76-77 0.3941615238903218 2315 78-79 0.6989512701471057 2315 80-81 0.677159316195258 2315 82-83 0.6049671547906854 2315 84-85 0.6613675966191863 2315 86-87 0.6583380540433694 2315 88-89 0.7143065303748095 2315 90-91 0.7871801935730964 2315 92-93 0.8229052524498606 2315 94-95 0.9145587054630475 2315 96-97 0.9397603880858796 2315 98-99 0.9708614296008804 2315 100 0.9502476965224709 2316 1 0.2971324846523018 2316 2 0.17258446187108234 2316 3 0.2519270250381993 2316 4 0.07035608822128836 2316 5 0.27914620123530653 2316 6 0.3511102528154879 2316 7 0.4452963088249362 2316 8 0.4638961753504631 2316 9 0.6652552629132344 2316 10-11 0.6780288134353825 2316 12-13 0.45675252848926107 2316 14-15 0.5687062411033921 2316 16-17 0.8288312491689211 2316 18-19 0.8094963813507263 2316 20-21 0.7646318579609144 2316 22-23 0.7885935245004276 2316 24-25 0.511223686903918 2316 26-27 0.7355857769860208 2316 28-29 0.98729907921755 2316 30-31 0.6015766917341381 2316 32-33 0.7447084702653726 2316 34-35 0.9242798526991791 2316 36-37 0.9076337318305789 2316 38-39 0.8879796644608433 2316 40-41 0.8241070703999895 2316 42-43 0.8152466244075356 2316 44-45 0.6776534715531355 2316 46-47 0.8182270822365254 2316 48-49 0.9065175045814655 2316 50-51 0.9423674047533126 2316 52-53 0.923352841304208 2316 54-55 0.8351197928820042 2316 56-57 0.9844645237634282 2316 58-59 0.9013986014868962 2316 60-61 1.0489406482015298 2316 62-63 1.075906295036333 2316 64-65 1.0724520484789721 2316 66-67 1.0397170985905717 2316 68-69 1.0836293261607466 2316 70-71 1.019075431131732 2316 72-73 1.0177009536333443 2316 74-75 1.0432255003406752 2316 76-77 0.8937897546462104 2316 78-79 0.6788351541993691 2316 80-81 0.7229465687707517 2316 82-83 0.6313061788309398 2316 84-85 0.7499099722264972 2316 86-87 0.7053152202678064 2316 88-89 0.691389756886732 2316 90-91 0.6386473448984873 2316 92-93 0.4001476751385198 2316 94-95 0.4624574561044348 2316 96-97 0.7205876020126105 2316 98-99 0.9115445308412617 2316 100 0.5822511694017578 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 3.0 12 25.0 13 123.0 14 511.0 15 1585.0 16 3617.0 17 7506.0 18 12762.0 19 18629.0 20 26240.0 21 36977.0 22 45825.0 23 47693.0 24 47385.0 25 50035.0 26 58957.0 27 71338.0 28 88448.0 29 108805.0 30 133702.0 31 167910.0 32 213527.0 33 281967.0 34 400277.0 35 560693.0 36 870247.0 37 1287069.0 38 1050580.0 39 152454.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.726488436170726 20.074771407007155 13.91717119719583 23.2815689596263 2 15.5953171715315 20.265456132948437 40.315416932989656 23.82380976253041 3 19.250904801890513 25.495117063749017 29.62535144190399 25.62862669245648 4 12.769763784792495 16.496094945081275 38.13211732455549 32.60202394557075 5 13.337629314606476 37.63754799035806 34.23987589696886 14.784946798066601 6 30.88768759234271 36.87553351631289 17.692427932651267 14.54435095869313 7 27.69579923911167 32.141248252352014 21.936306934332997 18.226645574203324 8 26.449058156585387 35.0458597741581 19.589117590128573 18.915964479127936 9 26.19803362204438 16.170905704766163 20.921764256431675 36.70929641675779 10-11 25.763645725338545 26.026305876749994 27.129993754434384 21.080054643477077 12-13 26.036444827702056 23.59446277857085 27.793628543423623 22.57546385030348 14-15 23.13615424891939 25.04926111804287 25.890007408315135 25.924577224722604 16-17 22.16521305474348 27.81301105244056 26.800675731542057 23.221100161273895 18-19 21.762957222609813 27.270107559673757 29.078971554616057 21.887963663100376 20-21 22.94381446093916 26.37201825931454 28.746409752574607 21.93775752717169 22-23 22.91825938257628 26.10114981903962 28.61208820895876 22.36850258942534 24-25 22.598127265394236 26.14017989295128 29.01084116743643 22.250851674218055 26-27 22.470837930907834 26.30749970800071 28.662232455107393 22.559429905984064 28-29 21.96386510761171 26.88899814539546 28.838608847287865 22.308527899704963 30-31 22.785190329970266 26.29766082482123 28.75364680160703 22.163502043601476 32-33 22.810008301689507 26.546273453665613 28.306924524785508 22.336793719859372 34-35 22.90036755168097 26.51901918894873 28.645299569419564 21.935313689950746 36-37 22.69983558609834 26.26798725955501 28.555443005574926 22.476734148771726 38-39 22.64358047156378 26.483622869804822 28.714988741014075 22.15780791761732 40-41 22.496353021750274 26.046087221637492 28.93923043253976 22.518329324072475 42-43 22.444943360217458 26.240752235634307 28.408317496103503 22.90598690804473 44-45 22.527060603304243 26.607343911097686 28.36862567257094 22.496969813027125 46-47 22.602199242440797 26.49712740158307 27.919581346386384 22.981092009589748 48-49 22.984031879667054 26.466747117247948 27.951369320825055 22.59785168225995 50-51 22.649136242242474 26.641747302220427 27.891742732441998 22.817373723095102 52-53 22.5357646649619 27.00174973847929 27.51294460043518 22.949540996123627 54-55 22.372209591823964 26.763137396150288 27.675006791516843 23.189646220508912 56-57 23.03723758270732 26.527718630923893 27.343131446662568 23.091912339706226 58-59 22.570237519831327 26.76815536427887 27.759906698429866 22.901700417459942 60-61 22.59254269082075 27.093389483271213 27.565457343646287 22.748610482261746 62-63 22.689128948190902 26.810016413887233 27.308072883960634 23.192781753961235 64-65 22.180382588575277 26.891842641263615 27.427536995281244 23.500237774879864 66-67 22.49165865254614 26.57230687449996 27.348402927947284 23.587631545006612 68-69 22.17149088503623 26.643955275878607 27.556109150181257 23.628444688903905 70-71 22.2610239916905 26.38527773999484 27.62662571364033 23.72707255467433 72-73 22.48783142496408 26.470085767773956 27.68801428011668 23.354068527145284 74-75 22.56829505875355 26.86956645902122 27.285086617995884 23.277051864229342 76-77 22.437030633806895 26.592679273845842 27.144110956363694 23.82617913598357 78-79 22.290407897719337 26.63750657638407 27.296955556352515 23.775129969544075 80-81 22.101113624669356 26.87507267386199 27.400239240612972 23.623574460855682 82-83 22.57251477181782 26.334800821635568 27.991079793239827 23.10160461330679 84-85 22.65237256493132 26.652673512943682 27.308663549613883 23.386290372511116 86-87 21.98290486345418 27.03036967936691 27.47196036726189 23.51476508991702 88-89 22.23833183704653 26.357806244968103 27.419560213611927 23.984301704373443 90-91 22.29746797911939 26.236881154516745 27.47099109755387 23.994659768809996 92-93 22.39186838444471 26.461489783282705 27.4427049127016 23.70393691957099 94-95 21.728955224712074 26.3589176411889 27.76938129723347 24.142745836865558 96-97 21.7267726916825 26.376768983905464 27.665578355773217 24.23087996863882 98-99 21.51467425396931 26.705824242539254 27.375757600026883 24.40374390346455 100 21.66978086394118 26.235732109733274 27.22785250284126 24.866634523484286 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 953.0 1 747.5 2 1108.0 3 1641.5 4 1206.5 5 897.5 6 1324.5 7 2463.5 8 3836.0 9 4474.0 10 4006.5 11 3393.5 12 3527.5 13 4057.5 14 4608.0 15 5334.5 16 6101.5 17 6825.5 18 7667.0 19 8432.0 20 9122.5 21 9983.5 22 11137.5 23 12724.0 24 14788.0 25 17369.0 26 20730.0 27 24970.5 28 30645.0 29 39237.0 30 50749.5 31 62559.0 32 77257.0 33 92755.5 34 105743.5 35 119421.0 36 133476.5 37 153474.5 38 165189.5 39 163012.5 40 160156.0 41 158012.0 42 161786.0 43 171331.5 44 183346.0 45 203106.0 46 221960.5 47 240056.0 48 260392.5 49 261176.5 50 247867.0 51 245180.5 52 242816.0 53 231572.5 54 220384.0 55 199878.5 56 176261.5 57 151100.0 58 127638.0 59 114399.0 60 94430.0 61 72244.0 62 56742.0 63 44748.5 64 32700.5 65 21246.5 66 14734.0 67 10706.5 68 7989.5 69 6067.0 70 4680.0 71 3540.0 72 2848.5 73 2275.5 74 1666.0 75 1200.0 76 825.5 77 504.0 78 303.0 79 185.5 80 129.0 81 88.0 82 53.0 83 33.0 84 23.0 85 14.5 86 9.5 87 6.0 88 3.5 89 1.5 90 3.0 91 2.5 92 1.5 93 1.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0452923147192712 2 0.0 3 0.0 4 0.03416941344885833 5 0.0 6 0.0 7 6.962692501040923E-4 8 0.0 9 0.01108808780790767 10-11 4.8738847507286457E-4 12-13 1.7406731252602306E-5 14-15 1.3925385002081845E-4 16-17 3.307278937994438E-4 18-19 0.0 20-21 8.703365626301153E-6 22-23 0.0 24-25 7.484894438618991E-4 26-27 4.6998174382026223E-4 28-29 2.3499087191013112E-4 30-31 0.002506569300374732 32-33 0.015535507642947557 34-35 0.026005656491387846 36-37 0.030923058070247998 38-39 0.022097845325178625 40-41 0.0013838351345818833 42-43 3.481346250520461E-5 44-45 0.010818283473492334 46-47 0.01849465195588995 48-49 0.003072288066084307 50-51 0.011192528195423282 52-53 0.010739953182855623 54-55 0.010539775773450697 56-57 3.916514531835519E-4 58-59 0.009060203616979502 60-61 0.004960918406991657 62-63 0.0012793947470662694 64-65 0.03615378081165499 66-67 0.06049709446841932 68-69 0.05985304541207303 70-71 0.052933869739163614 72-73 0.046197464744406516 74-75 0.03658894909297005 76-77 0.03226337637669838 78-79 0.015074229264753597 80-81 0.0010444038751561385 82-83 0.0385036895307563 84-85 0.05306442022355813 86-87 0.05897400548381662 88-89 0.07007950002297689 90-91 0.04926975281049083 92-93 0.04056638718418967 94-95 0.013307446042614461 96-97 0.0038642943380777124 98-99 0.022158768884562734 100 0.03221985954856687 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5744904.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.19746119005188 #Duplication Level Percentage of deduplicated Percentage of total 1 77.90697802860001 50.79337176453873 2 13.711445204280004 17.879028331311368 3 4.047882162131931 7.917349205024996 4 1.6285426696537362 4.247073900043716 5 0.8472274022770959 2.761853783955471 6 0.4638636103956573 1.8145637841748923 7 0.3303632157144167 1.5077190054612992 8 0.21712136143590627 1.132460922859898 9 0.14487633629874994 0.8501012381875186 >10 0.6675555018903129 7.284303755317934 >50 0.0244308146034035 1.0766400827711429 >100 0.008681220787821384 0.9859044791303891 >500 5.485012664577391E-4 0.2515882508102848 >1k 3.549118206257011E-4 0.4205892335677387 >5k 6.452942193183907E-5 0.36332986106703646 >10k+ 6.452942193183907E-5 0.7141224017776605 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18311 0.3187346559664008 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15637 0.27218905659694226 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9602 0.16713943348748733 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7670 0.1335096287074597 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.481346250520461E-5 5 0.0 0.0 0.0 0.0 3.481346250520461E-5 6 6.962692501040923E-5 0.0 0.0 0.0 3.481346250520461E-5 7 6.962692501040923E-5 0.0 0.0 0.0 3.481346250520461E-5 8 6.962692501040923E-5 0.0 0.0 0.0 3.481346250520461E-5 9 6.962692501040923E-5 0.0 0.0 1.7406731252602306E-5 3.481346250520461E-5 10-11 8.703365626301152E-5 0.0 0.0 7.833029063671038E-5 3.481346250520461E-5 12-13 1.2184711876821614E-4 8.703365626301153E-6 0.0 1.0444038751561383E-4 5.2220193757806916E-5 14-15 1.3925385002081845E-4 1.7406731252602306E-5 0.0 1.0444038751561383E-4 1.2184711876821614E-4 16-17 1.7406731252602307E-4 1.7406731252602306E-5 0.0 1.0444038751561383E-4 1.3925385002081845E-4 18-19 1.7406731252602307E-4 1.7406731252602306E-5 0.0 1.653639468997219E-4 1.5666058127342076E-4 20-21 1.7406731252602307E-4 1.7406731252602306E-5 0.0 2.785077000416369E-4 1.5666058127342076E-4 22-23 1.7406731252602307E-4 1.7406731252602306E-5 0.0 4.699817438202623E-4 2.0017740940492652E-4 24-25 1.7406731252602307E-4 1.7406731252602306E-5 0.0 0.0010618106064087408 2.523976031627334E-4 26-27 1.9147404377862538E-4 1.7406731252602306E-5 0.0 0.0019234438034125547 2.959144312942392E-4 28-29 1.9147404377862538E-4 1.7406731252602306E-5 0.0 0.004386496275655781 3.133211625468415E-4 30-31 1.9147404377862538E-4 1.7406731252602306E-5 0.0 0.014308333089639096 3.307278937994438E-4 32-33 2.0017740940492652E-4 1.7406731252602306E-5 0.0 0.03951327994340723 3.4813462505204614E-4 34-35 2.0888077503122766E-4 1.7406731252602306E-5 0.0 0.07496208813933183 3.4813462505204614E-4 36-37 2.0888077503122766E-4 1.7406731252602306E-5 0.0 0.1342842282482005 3.4813462505204614E-4 38-39 2.0888077503122766E-4 1.7406731252602306E-5 0.0 0.2204562513142082 3.4813462505204614E-4 40-41 2.0888077503122766E-4 1.7406731252602306E-5 0.0 0.3163934506129258 3.655413563046484E-4 42-43 2.0888077503122766E-4 1.7406731252602306E-5 0.0 0.4112862460364873 3.742447219309496E-4 44-45 2.175841406575288E-4 1.7406731252602306E-5 0.0 0.522428225084353 3.8294808755725076E-4 46-47 2.611009687890346E-4 1.7406731252602306E-5 0.0 0.6518733820443301 3.916514531835519E-4 48-49 2.611009687890346E-4 1.7406731252602306E-5 0.0 0.7907790974400964 4.1776155006245533E-4 50-51 2.8721106566793805E-4 1.7406731252602306E-5 0.0 0.9350199759647855 4.1776155006245533E-4 52-53 2.959144312942392E-4 1.7406731252602306E-5 0.0 1.072280755257181 4.351682813150576E-4 54-55 2.959144312942392E-4 1.7406731252602306E-5 0.0 1.2257559047113755 4.6127837819396114E-4 56-57 3.133211625468415E-4 1.7406731252602306E-5 1.7406731252602306E-5 1.4064377751133876 4.786851094465634E-4 58-59 3.307278937994438E-4 1.7406731252602306E-5 1.7406731252602306E-5 1.6116631365815688 4.960918406991657E-4 60-61 3.4813462505204614E-4 1.7406731252602306E-5 1.7406731252602306E-5 1.8169668283403866 5.047952063254669E-4 62-63 3.8294808755725076E-4 1.7406731252602306E-5 1.7406731252602306E-5 2.0270660745592965 5.222019375780692E-4 64-65 3.8294808755725076E-4 1.7406731252602306E-5 1.7406731252602306E-5 2.2346239380153263 5.222019375780692E-4 66-67 4.0035481880985305E-4 1.7406731252602306E-5 1.7406731252602306E-5 2.4738533489854664 5.309053032043704E-4 68-69 4.2646491568875647E-4 1.7406731252602306E-5 1.7406731252602306E-5 2.7420301540286838 5.396086688306715E-4 70-71 4.3516828131505767E-4 1.7406731252602306E-5 1.7406731252602306E-5 3.029223812965369 5.396086688306715E-4 72-73 4.699817438202623E-4 1.7406731252602306E-5 1.7406731252602306E-5 3.3079229870507847 6.26642325093683E-4 74-75 4.699817438202623E-4 1.7406731252602306E-5 1.7406731252602306E-5 3.5834280259513474 6.26642325093683E-4 76-77 4.699817438202623E-4 1.7406731252602306E-5 1.7406731252602306E-5 3.8913618051755083 6.26642325093683E-4 78-79 4.699817438202623E-4 1.7406731252602306E-5 1.7406731252602306E-5 4.232324856951482 6.26642325093683E-4 80-81 4.699817438202623E-4 1.7406731252602306E-5 1.7406731252602306E-5 4.592679007342856 6.527524219725865E-4 82-83 4.699817438202623E-4 1.7406731252602306E-5 1.7406731252602306E-5 4.962606859923159 6.962692501040923E-4 84-85 4.699817438202623E-4 1.7406731252602306E-5 1.7406731252602306E-5 5.318565810673251 6.962692501040923E-4 86-87 4.960918406991657E-4 1.7406731252602306E-5 1.7406731252602306E-5 5.690495437347604 6.962692501040923E-4 88 5.047952063254669E-4 1.7406731252602306E-5 1.7406731252602306E-5 5.996758170371515 6.962692501040923E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4435 0.0 33.58939 2 GTACTAG 3245 0.0 30.133633 1 GTACCTG 5420 0.0 28.189472 1 ACCTGGG 5230 0.0 28.128052 3 GTATCAA 27365 0.0 26.868544 1 GGTATCA 19675 0.0 26.546188 1 GTACATG 22115 0.0 25.913116 1 TACATGG 22340 0.0 25.36884 2 ACATGGG 22070 0.0 24.511505 3 TAGTACT 4780 0.0 24.384888 4 CTGTGCG 4745 0.0 23.964188 9 CATGGGG 17705 0.0 23.334038 4 CTAGTAC 4955 0.0 23.144253 3 CAACGCA 31145 0.0 23.067415 5 ATCAACG 31400 0.0 22.87129 3 TAGGCAT 4720 0.0 22.796766 5 GCATCGT 3940 0.0 22.778265 8 CCTGGGG 5235 0.0 22.355257 4 TATCAAC 32770 0.0 22.241898 2 CTGGGGG 3815 0.0 22.04643 5 >>END_MODULE