FastQCFastQC Report
Mon 6 Jun 2016
ERR1211262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062940
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168300.4142320585585807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135990.3347083638941259No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80980.19931379739794333No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45030.11083107306531723No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77950.051.4352261
TACCTGG36850.044.5676542
GTATCAA138650.040.490931
ACCTGGG43500.037.0669823
GTACCTG45450.036.2385941
TATCACG3700.034.3394932
ATCAACG167050.032.7839933
CAACGCA168200.032.5594445
TATCAAC173250.032.078092
GTGGTAT30850.030.20281
AACGCAG184750.030.1269386
TCAACGC182400.030.0762064
GTACTAG23000.028.8488461
GTACATG181950.028.7600691
GTACACG7600.028.4827181
TACATGG181350.028.0504592
CCTGGGG41700.027.3934844
ACATGGG180700.027.0035933
ACGCAGA204900.026.705387
CGCAGAG209600.026.0600878
Produced by FastQC (version 0.11.5)