##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211262_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4062940 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.363090274530265 31.0 31.0 34.0 28.0 34.0 2 31.35683322913949 31.0 31.0 34.0 28.0 34.0 3 31.416733695304387 31.0 31.0 34.0 28.0 34.0 4 35.02302889040941 37.0 35.0 37.0 32.0 37.0 5 35.084254259231 37.0 35.0 37.0 32.0 37.0 6 35.14822197718893 37.0 35.0 37.0 32.0 37.0 7 35.0728201745534 37.0 35.0 37.0 32.0 37.0 8 35.07603779529109 37.0 35.0 37.0 32.0 37.0 9 36.62635259196542 39.0 37.0 39.0 32.0 39.0 10-11 36.56802463240905 39.0 37.0 39.0 32.0 39.0 12-13 36.45790302096512 39.0 37.0 39.0 32.0 39.0 14-15 37.621355717780716 40.0 37.0 41.0 32.0 41.0 16-17 37.59396040798043 40.0 37.0 41.0 32.0 41.0 18-19 37.620808700103865 40.0 37.0 41.0 32.0 41.0 20-21 37.59089083274673 40.0 37.0 41.0 32.0 41.0 22-23 37.47697197103575 40.0 37.0 41.0 31.0 41.0 24-25 37.37714782891207 40.0 37.0 41.0 31.0 41.0 26-27 37.22039717544438 40.0 36.0 41.0 31.0 41.0 28-29 37.09399769624952 40.0 36.0 41.0 30.5 41.0 30-31 36.91995636164945 39.0 36.0 41.0 30.0 41.0 32-33 36.70399144461892 39.0 35.0 41.0 30.0 41.0 34-35 36.53892439957272 39.0 35.0 41.0 30.0 41.0 36-37 36.419668146711494 39.0 35.0 41.0 29.5 41.0 38-39 36.27250057839889 39.0 35.0 40.5 29.0 41.0 40-41 36.10200076791683 39.0 35.0 40.0 28.0 41.0 42-43 35.89134801399972 38.0 35.0 40.0 27.5 41.0 44-45 35.67021024184457 38.0 34.5 40.0 27.0 41.0 46-47 35.42112792706759 38.0 34.0 40.0 26.0 41.0 48-49 35.19716486091353 38.0 34.0 40.0 25.0 41.0 50-51 34.51775204654757 37.5 33.5 39.5 24.0 40.5 52-53 34.62448067163187 38.0 33.5 39.5 24.0 40.5 54-55 35.10833362048172 38.0 34.0 40.0 26.0 41.0 56-57 35.07496701895671 38.0 34.0 40.0 25.5 41.0 58-59 34.87098135832673 38.0 34.0 40.0 25.0 41.0 60-61 34.646269696328275 37.0 33.5 40.0 25.0 41.0 62-63 34.344018493996955 37.0 33.0 40.0 24.5 41.0 64-65 34.100302982569275 36.0 33.0 40.0 24.0 41.0 66-67 33.92972441138683 36.0 33.0 39.0 24.0 41.0 68-69 33.63047792977499 35.5 33.0 39.0 23.0 41.0 70-71 33.32222306999365 35.0 32.0 39.0 23.0 40.5 72-73 32.97597835557502 35.0 32.0 37.5 23.0 40.0 74-75 32.64257335820859 35.0 32.0 37.0 22.0 39.5 76-77 32.308272950129705 35.0 31.0 36.5 21.5 39.0 78-79 31.975585167391102 35.0 31.0 36.0 20.0 39.0 80-81 31.68455490359198 35.0 31.0 36.0 20.0 37.5 82-83 31.394907505402493 35.0 31.0 35.0 20.0 37.0 84-85 31.142050091805444 34.0 30.5 35.0 19.5 37.0 86-87 30.91478178363451 34.0 30.0 35.0 19.0 36.0 88-89 30.70668112253688 34.0 30.0 35.0 18.5 36.0 90-91 30.4801274446583 34.0 30.0 35.0 18.0 36.0 92-93 30.28362072784732 34.0 29.0 35.0 18.0 35.0 94-95 30.107472052257727 34.0 29.0 35.0 17.0 35.0 96-97 29.927172933885316 34.0 29.0 35.0 16.5 35.0 98-99 29.698314274884687 34.0 29.0 35.0 15.0 35.0 100 28.582691351582845 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4800135727447419 1101 2 -0.3905882559979972 1101 3 -0.487052826515054 1101 4 -0.36766919042388935 1101 5 -0.26471897823223856 1101 6 -0.39406430612302756 1101 7 -0.3340592123667534 1101 8 -0.2573799409171471 1101 9 -0.4652552893258388 1101 10-11 -0.4936727058775787 1101 12-13 -0.5624612627435539 1101 14-15 -0.6080931566944159 1101 16-17 -0.6688597273648185 1101 18-19 -0.6740778229240902 1101 20-21 -0.666285120006421 1101 22-23 -0.6772556465959951 1101 24-25 -0.7585909907155823 1101 26-27 -0.7494134799659165 1101 28-29 -0.6936955810694485 1101 30-31 -0.9336303348849242 1101 32-33 -0.9603581089277782 1101 34-35 -0.9127866574720471 1101 36-37 -0.9698176408453563 1101 38-39 -1.0097275668023258 1101 40-41 -0.9632080471607694 1101 42-43 -1.0003760010192977 1101 44-45 -0.9544880648397651 1101 46-47 -1.0828675102746388 1101 48-49 -1.0941873183167132 1101 50-51 -1.0840561866846912 1101 52-53 -0.9663325554237119 1101 54-55 -0.9706488688511072 1101 56-57 -0.8534591674400787 1101 58-59 -0.7863338856658899 1101 60-61 -0.9176289229918453 1101 62-63 -0.8639803371609247 1101 64-65 -0.7845550249633035 1101 66-67 -0.8765271499817047 1101 68-69 -0.7363409587183014 1101 70-71 -0.87265888533382 1101 72-73 -0.9220529513720805 1101 74-75 -1.0657698895777017 1101 76-77 -1.0509255917737832 1101 78-79 -0.9584561055758165 1101 80-81 -1.0210677979768015 1101 82-83 -1.0306992054087907 1101 84-85 -1.1650477575388969 1101 86-87 -0.9933171834343568 1101 88-89 -1.139625993090199 1101 90-91 -1.1907813554415938 1101 92-93 -1.3253928324214534 1101 94-95 -1.2114901916892507 1101 96-97 -1.2566666807906195 1101 98-99 -1.3713750847336748 1101 100 -1.3250951532855488 1102 1 -0.516687503321215 1102 2 -0.5032520773340003 1102 3 -0.5002851061255704 1102 4 -0.3952511870900608 1102 5 -0.3242743904805323 1102 6 -0.4003936057622042 1102 7 -0.395513464240679 1102 8 -0.41204502052036673 1102 9 -0.5084453531592743 1102 10-11 -0.6030591058159089 1102 12-13 -0.5303276211706631 1102 14-15 -0.6466809713959236 1102 16-17 -0.6705123842731524 1102 18-19 -0.7421324240956508 1102 20-21 -0.7651699205859899 1102 22-23 -0.7861057080587841 1102 24-25 -0.7529572608650525 1102 26-27 -0.835861825941322 1102 28-29 -0.8604831287000039 1102 30-31 -0.8074644276270107 1102 32-33 -0.843704759889583 1102 34-35 -0.9423213721363268 1102 36-37 -0.7583262543393232 1102 38-39 -0.8363905227687667 1102 40-41 -1.0269404009576633 1102 42-43 -0.970617359550026 1102 44-45 -1.1179280248537253 1102 46-47 -1.1171753928463204 1102 48-49 -1.2288084000120705 1102 50-51 -1.1185511515435635 1102 52-53 -1.0667705037021378 1102 54-55 -0.7960858017294257 1102 56-57 -0.8661843272308971 1102 58-59 -0.9128175509867091 1102 60-61 -0.7867230451929643 1102 62-63 -0.8472293024889552 1102 64-65 -0.8971185910816004 1102 66-67 -0.9925942608824485 1102 68-69 -0.949013980822528 1102 70-71 -0.9415710644155837 1102 72-73 -1.06921588205714 1102 74-75 -0.9660069958614983 1102 76-77 -1.1221678446854924 1102 78-79 -1.0469229840535625 1102 80-81 -1.0978516487221057 1102 82-83 -1.0969913621268823 1102 84-85 -1.1334408392243098 1102 86-87 -1.130530395074402 1102 88-89 -1.1639026759397204 1102 90-91 -1.1744233013294725 1102 92-93 -1.1101523400891757 1102 94-95 -1.291377146574817 1102 96-97 -1.3116331062755862 1102 98-99 -1.3308210409960388 1102 100 -1.4124247007652428 1103 1 -0.6233183762353356 1103 2 -0.47161072110390023 1103 3 -0.5977524573678181 1103 4 -0.39899669029926343 1103 5 -0.40801732552811387 1103 6 -0.40679314349311824 1103 7 -0.5061659177378957 1103 8 -0.5016208926419736 1103 9 -0.6685329114194261 1103 10-11 -0.6678835177971862 1103 12-13 -0.675833839933901 1103 14-15 -0.8582986925136282 1103 16-17 -0.9561437206601866 1103 18-19 -0.8370633144652473 1103 20-21 -0.8138050831325572 1103 22-23 -0.9655694438110061 1103 24-25 -0.9322193558027649 1103 26-27 -0.9394706949269604 1103 28-29 -1.0768858565691701 1103 30-31 -1.0121296537155189 1103 32-33 -1.0769486088415547 1103 34-35 -1.0698119371519468 1103 36-37 -0.9786969925453448 1103 38-39 -0.9513468539893921 1103 40-41 -1.0087716607011217 1103 42-43 -1.2113024567890562 1103 44-45 -1.0682730212522813 1103 46-47 -0.9917445737030803 1103 48-49 -0.9836195899737703 1103 50-51 -1.1053508008289867 1103 52-53 -1.081394980288728 1103 54-55 -0.8624832127014983 1103 56-57 -0.8002208954446814 1103 58-59 -0.9032104195229138 1103 60-61 -0.9385485466365537 1103 62-63 -0.8254073523313465 1103 64-65 -0.6467990756615905 1103 66-67 -0.7957360454738804 1103 68-69 -0.803469038997406 1103 70-71 -0.8933415911495501 1103 72-73 -0.8277551197545421 1103 74-75 -0.8809906137993018 1103 76-77 -0.8993932670778548 1103 78-79 -1.011808791148507 1103 80-81 -1.0940363236189263 1103 82-83 -1.0046326461274369 1103 84-85 -1.0778872900450196 1103 86-87 -1.0939505443086688 1103 88-89 -1.1623489381610597 1103 90-91 -1.2917732394327643 1103 92-93 -1.3664384172423674 1103 94-95 -1.4005194006582968 1103 96-97 -1.5170501215501275 1103 98-99 -1.5666107807891017 1103 100 -1.8130025371292042 1104 1 -0.4921262616394877 1104 2 -0.43327613630112793 1104 3 -0.46070014436364914 1104 4 -0.3400045957111075 1104 5 -0.3698561671063203 1104 6 -0.4799944972153156 1104 7 -0.5933274229333705 1104 8 -0.44568570491957615 1104 9 -0.48207361304103813 1104 10-11 -0.5539246711735686 1104 12-13 -0.608619887690601 1104 14-15 -0.7130495512430954 1104 16-17 -0.9384562068455864 1104 18-19 -0.8461691089071408 1104 20-21 -0.8053312708719602 1104 22-23 -0.7400481662603937 1104 24-25 -0.8168672460783597 1104 26-27 -0.81531156178999 1104 28-29 -0.8498236306617386 1104 30-31 -1.0131456966442727 1104 32-33 -0.9595858839675273 1104 34-35 -0.966138666759818 1104 36-37 -0.9777568681417605 1104 38-39 -0.801759245790663 1104 40-41 -0.9303214899437577 1104 42-43 -0.8759423360786016 1104 44-45 -0.977876161115951 1104 46-47 -1.044354806808542 1104 48-49 -1.0182511696287122 1104 50-51 -1.0691093925719812 1104 52-53 -0.9076109914266084 1104 54-55 -0.7613499310085743 1104 56-57 -0.8215399640034562 1104 58-59 -0.8741727698655595 1104 60-61 -0.822491739018453 1104 62-63 -0.8602121211218758 1104 64-65 -0.6514955063442258 1104 66-67 -0.8226015158702111 1104 68-69 -0.7760399130924611 1104 70-71 -0.6562480369882024 1104 72-73 -0.7333180101467818 1104 74-75 -0.77564923152066 1104 76-77 -0.639800071750166 1104 78-79 -0.7086704695726631 1104 80-81 -0.9306922391152845 1104 82-83 -0.9377423756795515 1104 84-85 -0.9557088791383741 1104 86-87 -0.8563715465360104 1104 88-89 -1.0338311326967435 1104 90-91 -1.0468062620141794 1104 92-93 -1.073796108560014 1104 94-95 -0.9725356345695992 1104 96-97 -1.0529172752025033 1104 98-99 -1.2250357798104652 1104 100 -1.2044225575560326 1105 1 -0.6073226274444004 1105 2 -0.5169240274725517 1105 3 -0.5833927497572127 1105 4 -0.4765169664899247 1105 5 -0.4197645471660678 1105 6 -0.4884816428155503 1105 7 -0.3917397370944613 1105 8 -0.470903148633262 1105 9 -0.7086658421598884 1105 10-11 -0.6898410739036862 1105 12-13 -0.6556372636590595 1105 14-15 -0.831120991898203 1105 16-17 -0.7828829674498508 1105 18-19 -0.8500243681643482 1105 20-21 -0.8803541672414426 1105 22-23 -0.8714826319457813 1105 24-25 -0.9693121510072089 1105 26-27 -0.8996248433997422 1105 28-29 -0.856155925199225 1105 30-31 -0.9553646720923439 1105 32-33 -0.9137412217911205 1105 34-35 -0.9841628710884791 1105 36-37 -0.9280423193761962 1105 38-39 -0.8616380288609733 1105 40-41 -0.9769708085436619 1105 42-43 -0.9734094948464929 1105 44-45 -1.0337007991641514 1105 46-47 -1.0646965625494005 1105 48-49 -1.1477990158230043 1105 50-51 -1.0488757335801182 1105 52-53 -0.8639665939666159 1105 54-55 -0.8491618253575766 1105 56-57 -0.6759364383722755 1105 58-59 -0.615375674164433 1105 60-61 -0.6866510384749276 1105 62-63 -0.6432860311922752 1105 64-65 -0.6625718428669032 1105 66-67 -0.7563755811817785 1105 68-69 -0.7747865958088624 1105 70-71 -0.7402980649141568 1105 72-73 -0.6888441051155283 1105 74-75 -0.7394599103460386 1105 76-77 -0.7277414677658811 1105 78-79 -0.8179230458402209 1105 80-81 -0.788979200870628 1105 82-83 -0.8955713181456595 1105 84-85 -0.9603064563467534 1105 86-87 -0.9657912649606359 1105 88-89 -1.092614703859521 1105 90-91 -1.0781646196236494 1105 92-93 -1.092571859078081 1105 94-95 -1.0966188703682391 1105 96-97 -1.0405066732816657 1105 98-99 -1.0141803389752262 1105 100 -1.0607003671965494 1106 1 -0.5278434222160797 1106 2 -0.5707952391175333 1106 3 -0.6522971127827262 1106 4 -0.4193235592811959 1106 5 -0.37867485014133706 1106 6 -0.378520396549483 1106 7 -0.4291529078926075 1106 8 -0.4175241437509172 1106 9 -0.6398801471653996 1106 10-11 -0.5358782164880935 1106 12-13 -0.6395590914756042 1106 14-15 -0.7702810710703716 1106 16-17 -0.905283948400097 1106 18-19 -0.9885830456131472 1106 20-21 -0.6791609605533111 1106 22-23 -0.7416290294433736 1106 24-25 -0.7682090912782655 1106 26-27 -0.8046105304781861 1106 28-29 -0.8211027639585424 1106 30-31 -0.8610957632037781 1106 32-33 -0.8302484032805921 1106 34-35 -0.7989464849178276 1106 36-37 -0.960398347624853 1106 38-39 -0.8966980657171817 1106 40-41 -0.910490190940223 1106 42-43 -0.7587711493131266 1106 44-45 -0.9518376106712978 1106 46-47 -0.9421905192835212 1106 48-49 -1.0085763139811235 1106 50-51 -0.976128010609365 1106 52-53 -0.9894767719148234 1106 54-55 -0.7904780466298789 1106 56-57 -0.7756127460343194 1106 58-59 -0.8283936663042581 1106 60-61 -0.8441497448405997 1106 62-63 -0.8811373642322451 1106 64-65 -0.863717284035495 1106 66-67 -0.9226631795212725 1106 68-69 -0.9270186170564756 1106 70-71 -0.8351691904138576 1106 72-73 -0.8115266225133908 1106 74-75 -0.7942962910685871 1106 76-77 -0.7855449494320723 1106 78-79 -0.7597109197863965 1106 80-81 -0.7365553244844456 1106 82-83 -0.6236199878995912 1106 84-85 -0.8343313558245278 1106 86-87 -0.7777852705880193 1106 88-89 -0.814852860047889 1106 90-91 -0.8431641568838089 1106 92-93 -0.8640315282452882 1106 94-95 -0.9622941896878316 1106 96-97 -1.049704900842567 1106 98-99 -1.0281958992571312 1106 100 -1.0678251055593684 1107 1 -0.6391751762108768 1107 2 -0.7571386058956797 1107 3 -0.7040181272754822 1107 4 -0.47150211743801407 1107 5 -0.42562533323312124 1107 6 -0.48154204277764734 1107 7 -0.47018551658825913 1107 8 -0.4638243109749993 1107 9 -0.614801180238608 1107 10-11 -0.6790313194238067 1107 12-13 -0.7178849926275888 1107 14-15 -0.917755290691332 1107 16-17 -0.9047427926958989 1107 18-19 -0.9501969902433913 1107 20-21 -0.8008745428647188 1107 22-23 -0.8459501461286294 1107 24-25 -0.8431661239798629 1107 26-27 -0.8679687151678976 1107 28-29 -1.0499779497630826 1107 30-31 -0.9940261329548008 1107 32-33 -1.1190815508093905 1107 34-35 -0.9514385882251446 1107 36-37 -0.9746205233959486 1107 38-39 -0.9649487160330494 1107 40-41 -1.00661036002235 1107 42-43 -0.9180288602012752 1107 44-45 -1.0664790079213944 1107 46-47 -0.9934191517621542 1107 48-49 -1.0247970497670806 1107 50-51 -1.0307127374766978 1107 52-53 -0.8648588324871014 1107 54-55 -0.7987080261913704 1107 56-57 -0.8997441098395953 1107 58-59 -0.9400494169545794 1107 60-61 -0.9720053282668388 1107 62-63 -0.7679440810661404 1107 64-65 -0.7655695229801225 1107 66-67 -0.7780131888114994 1107 68-69 -0.778241443143429 1107 70-71 -0.8104989934610529 1107 72-73 -0.8348067692989716 1107 74-75 -0.7537607095003978 1107 76-77 -0.8218627680868451 1107 78-79 -0.8288093032869561 1107 80-81 -0.82027768606007 1107 82-83 -0.7868899154358182 1107 84-85 -0.6906487099679701 1107 86-87 -0.7764149621524936 1107 88-89 -0.8825623546595587 1107 90-91 -0.8733028135170322 1107 92-93 -0.9308967735072748 1107 94-95 -0.8606033684317929 1107 96-97 -1.003793761860777 1107 98-99 -1.0001998011523376 1107 100 -1.0600306053735657 1108 1 -0.5732403510875841 1108 2 -0.48051383972596184 1108 3 -0.5303963087844643 1108 4 -0.4801223062028228 1108 5 -0.35000072295026285 1108 6 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1.1056945222669086 2311 60-61 1.1042953204925539 2311 62-63 1.1278192071196145 2311 64-65 0.9898136149666072 2311 66-67 1.032768310164144 2311 68-69 1.0556711735194426 2311 70-71 0.9899005057422272 2311 72-73 0.9583825375528434 2311 74-75 1.089181328969893 2311 76-77 1.07809938761541 2311 78-79 1.0621045084590044 2311 80-81 1.04273733784159 2311 82-83 1.0584044449101846 2311 84-85 1.0862070833395627 2311 86-87 1.0829671514373125 2311 88-89 1.1490811691282836 2311 90-91 1.1758704450761854 2311 92-93 1.2098971031146242 2311 94-95 1.287090263482618 2311 96-97 1.2881909296939682 2311 98-99 1.3819106586701047 2311 100 1.5186377306155094 2312 1 0.6549694656797946 2312 2 0.5821744917048974 2312 3 0.6690808263597354 2312 4 0.4151240326612111 2312 5 0.5275780313708793 2312 6 0.5892465644747418 2312 7 0.45780248629826303 2312 8 0.5091934591855605 2312 9 0.6912379682369476 2312 10-11 0.6606222977510114 2312 12-13 0.8151138347222258 2312 14-15 1.0083778953090246 2312 16-17 1.0334647438366602 2312 18-19 0.9924513951075014 2312 20-21 0.9899613086597299 2312 22-23 1.053690729750862 2312 24-25 1.0471301957026213 2312 26-27 1.123294477146736 2312 28-29 1.099133542208783 2312 30-31 1.0601948439530133 2312 32-33 1.2244418119905376 2312 34-35 1.2296969656265588 2312 36-37 1.2829408152293453 2312 38-39 1.2758989747481166 2312 40-41 1.324089396427226 2312 42-43 1.2800954632697739 2312 44-45 1.2110489552754373 2312 46-47 1.3718687113083874 2312 48-49 1.4117715953709364 2312 50-51 1.3720892199470782 2312 52-53 1.3355453935637698 2312 54-55 1.1994275727622608 2312 56-57 1.1005169175823752 2312 58-59 1.1132785417320505 2312 60-61 1.1634008146160753 2312 62-63 1.0499337603554721 2312 64-65 1.1248728142019004 2312 66-67 1.0465352281107485 2312 68-69 1.0206214244373015 2312 70-71 0.9705942773309388 2312 72-73 1.065988715997797 2312 74-75 1.1526826650260276 2312 76-77 1.1305693535079584 2312 78-79 1.150194056806857 2312 80-81 1.0779407876906113 2312 82-83 1.2058751711598852 2312 84-85 1.1618521920706364 2312 86-87 1.1973509362779637 2312 88-89 1.1658004452682782 2312 90-91 1.202120580854249 2312 92-93 1.1848370348997257 2312 94-95 1.2871770528202404 2312 96-97 1.3423872321857715 2312 98-99 0.9264994140740725 2312 100 1.2678604283626775 2313 1 0.5256414390799939 2313 2 0.5551705270481513 2313 3 0.5786815214574474 2313 4 0.4496059419906402 2313 5 0.2441668188153372 2313 6 0.4202629054283804 2313 7 0.5306832421486121 2313 8 0.5052852449230585 2313 9 0.7127257920543073 2313 10-11 0.7260826631562125 2313 12-13 0.8595429617793755 2313 14-15 0.9980080431199099 2313 16-17 0.9511202086661541 2313 18-19 0.9893035294884953 2313 20-21 0.9563753809676712 2313 22-23 1.0519905282683055 2313 24-25 1.0473528014296463 2313 26-27 0.9937883146539619 2313 28-29 1.0504799519538253 2313 30-31 1.0918025481807234 2313 32-33 1.1241126496995903 2313 34-35 1.215201681139348 2313 36-37 1.1419952155194224 2313 38-39 1.1837839894402649 2313 40-41 1.2653722827247051 2313 42-43 1.340542795592846 2313 44-45 1.3166194006237149 2313 46-47 1.338936062582448 2313 48-49 1.278712036580103 2313 50-51 1.2283944437842749 2313 52-53 1.1160590543547215 2313 54-55 1.0118175526824231 2313 56-57 0.9687909406998543 2313 58-59 1.026935398809428 2313 60-61 0.9250996306922659 2313 62-63 0.8585034394543101 2313 64-65 0.9091933493450455 2313 66-67 0.9407190014103719 2313 68-69 0.9508925535947981 2313 70-71 0.9740613850471362 2313 72-73 1.0320861972948165 2313 74-75 0.9747377357843803 2313 76-77 0.8026652167871617 2313 78-79 0.9160687613390159 2313 80-81 0.9810189553966104 2313 82-83 1.0965641286159418 2313 84-85 1.0762681150078386 2313 86-87 1.1038378022835076 2313 88-89 1.1123595063390432 2313 90-91 1.149411598289845 2313 92-93 1.2085835842116417 2313 94-95 1.2510900390380115 2313 96-97 1.2374256814884461 2313 98-99 1.2744872396018074 2313 100 1.331940433014907 2314 1 0.6333504810441148 2314 2 0.6314936861086302 2314 3 0.6387042136999099 2314 4 0.5247905022958435 2314 5 0.5582178782711367 2314 6 0.5806016794625819 2314 7 0.6013163143520046 2314 8 0.6279115999834843 2314 9 0.7765506931899822 2314 10-11 0.7816507715275023 2314 12-13 0.8951594711872488 2314 14-15 1.041548503034413 2314 16-17 1.048349245999141 2314 18-19 1.0040459290988721 2314 20-21 1.0731605724231912 2314 22-23 1.1482016039962133 2314 24-25 1.1020236061097606 2314 26-27 1.0894154110618572 2314 28-29 1.106446329030426 2314 30-31 1.1467860127932994 2314 32-33 1.151196769631433 2314 34-35 1.1456100335323853 2314 36-37 1.1427400097668468 2314 38-39 1.0669924858171669 2314 40-41 1.1696021451244363 2314 42-43 1.2827874796264993 2314 44-45 1.2425890741467853 2314 46-47 1.2740708996180672 2314 48-49 1.235721877249361 2314 50-51 1.2442613643551184 2314 52-53 1.191897879135432 2314 54-55 1.0899009136591715 2314 56-57 1.0136665694042009 2314 58-59 1.0039005136186248 2314 60-61 1.0826284727301 2314 62-63 1.0426974105369453 2314 64-65 1.017020474523143 2314 66-67 1.050309360006274 2314 68-69 0.9871902787726583 2314 70-71 1.0204419258636932 2314 72-73 1.0649731510244678 2314 74-75 1.0187282906222492 2314 76-77 0.9647189693790992 2314 78-79 1.0422228290576108 2314 80-81 1.0329021490676986 2314 82-83 1.0841844314704154 2314 84-85 1.1606538638609756 2314 86-87 1.2336175666037832 2314 88-89 1.3112628212070696 2314 90-91 1.3252260007958405 2314 92-93 1.4349517008551835 2314 94-95 1.348348664656541 2314 96-97 1.4211034667614655 2314 98-99 1.4735915264107575 2314 100 1.4910810995033934 2315 1 0.36905871556416514 2315 2 0.44161274517297144 2315 3 0.45672286850569677 2315 4 0.13752934067014166 2315 5 0.20017550181447064 2315 6 0.2931317395172499 2315 7 0.3331516229814895 2315 8 0.36644006265213847 2315 9 0.4711817004140926 2315 10-11 0.4276324998293646 2315 12-13 0.44798134745818174 2315 14-15 0.6573551918580876 2315 16-17 0.6626874093542341 2315 18-19 0.5591994743150295 2315 20-21 0.6161322517031138 2315 22-23 0.4645399213376393 2315 24-25 0.6321934346129368 2315 26-27 0.7494401312809771 2315 28-29 0.7505764242127242 2315 30-31 0.7522092885786194 2315 32-33 0.8433151578130449 2315 34-35 0.8353528634327461 2315 36-37 0.855002310535923 2315 38-39 0.8616658110057571 2315 40-41 0.8998810890205746 2315 42-43 0.965946484974971 2315 44-45 1.0406541987310973 2315 46-47 1.0094201625922992 2315 48-49 0.9609042856117469 2315 50-51 0.8814693255615467 2315 52-53 0.9526312959951682 2315 54-55 0.7974648539383296 2315 56-57 0.7708846382863399 2315 58-59 0.8105181408362938 2315 60-61 0.7893538903796511 2315 62-63 0.784636133187206 2315 64-65 0.7909959079671935 2315 66-67 0.803134718339571 2315 68-69 0.73263956257464 2315 70-71 0.721810723773352 2315 72-73 0.5067517755565163 2315 74-75 0.7061276965872594 2315 76-77 0.7389891460723561 2315 78-79 0.7132205901965385 2315 80-81 0.506140500195638 2315 82-83 0.5071271385371929 2315 84-85 0.7476915675676814 2315 86-87 0.7270852862698085 2315 88-89 0.915289010355604 2315 90-91 0.9867475028945307 2315 92-93 1.0221256978152056 2315 94-95 1.010990304208633 2315 96-97 0.9022187115536795 2315 98-99 0.4234258133659843 2315 100 0.9001398907737119 2316 1 0.3120740892130591 2316 2 0.34349863883913656 2316 3 0.4055007902186638 2316 4 0.2470263214582218 2316 5 0.16454468871773997 2316 6 0.23276463624718247 2316 7 0.22297266356435586 2316 8 0.3001202112480854 2316 9 0.3489515960268079 2316 10-11 0.35725933071922356 2316 12-13 0.21386219653871308 2316 14-15 0.2100937301619865 2316 16-17 0.36126656342657526 2316 18-19 0.4223659456410829 2316 20-21 0.44167423622665325 2316 22-23 0.44433673283052144 2316 24-25 0.4126228755275392 2316 26-27 0.4467187281185119 2316 28-29 0.43063334715257184 2316 30-31 0.3119252564742183 2316 32-33 0.5742934373077659 2316 34-35 0.5693606359329024 2316 36-37 0.5545744460543318 2316 38-39 0.6751378019035741 2316 40-41 0.715413580675758 2316 42-43 0.6479602410665208 2316 44-45 0.5822386167188824 2316 46-47 0.4648576570233729 2316 48-49 0.4632732189149209 2316 50-51 0.4418741261427712 2316 52-53 0.5081380163976377 2316 54-55 0.28451325728584465 2316 56-57 0.21866957855323932 2316 58-59 0.29182969883189713 2316 60-61 0.36870244657263385 2316 62-63 0.49057054671985156 2316 64-65 0.42119128284981144 2316 66-67 0.4513057133429683 2316 68-69 0.34148540798824456 2316 70-71 0.08712508507809247 2316 72-73 -0.05660712340783647 2316 74-75 0.14357329251320294 2316 76-77 0.32586431313998077 2316 78-79 0.3153828736940234 2316 80-81 0.5274112080140618 2316 82-83 0.5864774082443169 2316 84-85 0.6063489264255217 2316 86-87 0.7140497274243067 2316 88-89 0.6821594138539133 2316 90-91 0.5262517147122949 2316 92-93 0.6324434518779469 2316 94-95 0.8193440283602698 2316 96-97 0.6899374416340791 2316 98-99 0.5984501452668205 2316 100 0.54549729555373 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 10.0 1 240.0 2 77.0 3 90.0 4 80.0 5 49.0 6 77.0 7 406.0 8 1279.0 9 2139.0 10 2986.0 11 4652.0 12 6816.0 13 8700.0 14 10574.0 15 12704.0 16 15746.0 17 19610.0 18 24148.0 19 29571.0 20 34621.0 21 41407.0 22 45830.0 23 46989.0 24 45889.0 25 46293.0 26 50393.0 27 57104.0 28 66185.0 29 78556.0 30 94695.0 31 116499.0 32 147464.0 33 191025.0 34 270777.0 35 370235.0 36 546810.0 37 783360.0 38 705663.0 39 182979.0 40 212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.58305392369931 18.878084639262973 12.578026397284175 22.960835039753537 2 16.094285686823905 19.98507485860425 40.94287165536725 22.977767799204603 3 19.350719779985788 26.687037806080966 29.098046591042525 24.864195822890725 4 12.61683979959102 16.36531387568548 38.369881246872154 32.64796507785135 5 13.106278433148866 37.49859680564035 34.0870128207653 15.308111940445476 6 29.786465414430165 36.3896296358316 19.151525509124834 14.672379440613408 7 27.337887304242297 32.3935535158074 22.480170574432748 17.788388605517564 8 26.51265733325519 34.51235494214923 20.390542545788367 18.58444517880721 9 27.10761985687915 16.04506708774456 21.15768679319522 35.689626262181065 10-11 25.61780744053991 25.84572378921979 27.738058583107104 20.798410187133197 12-13 25.84842661627919 23.881470746072743 27.982987824298295 22.287114813349778 14-15 23.23237072596012 24.99613818919168 26.51647925447542 25.255011830372776 16-17 22.051865281721604 27.5559879918912 27.34060269480867 23.05154403157852 18-19 21.52473349836446 27.07901308235998 29.653254933701994 21.742998485573562 20-21 22.847987517818634 26.440836604518758 29.241228174368967 21.469947703293645 22-23 22.79844280115581 26.232505454947415 29.019064681163464 21.94998706273331 24-25 22.222778878575973 26.053634564568284 29.64674738238504 22.076839174470695 26-27 22.059574723687575 26.336130614286642 29.179273878742805 22.425020783282978 28-29 21.73817946658005 26.89370828252472 29.335020259235 22.03309199166023 30-31 22.703693419451906 26.425865013615418 29.31163974353483 21.558801823397843 32-33 22.621391191210783 26.533752582611246 28.84121149008899 22.003644736088983 34-35 22.58782419626554 26.645645859300586 29.126119181970882 21.640410762462988 36-37 22.420867916000123 26.56800605415157 28.801200687542067 22.20992534230624 38-39 22.439490148720367 26.823801019500692 28.899716931187953 21.83699190059099 40-41 22.43829429371468 26.064121545802475 29.335044665827333 22.162539494655512 42-43 22.347871806238278 26.31692525830337 28.714203112356966 22.620999823101386 44-45 22.200771606591196 26.939453493140626 28.75135133338332 22.108423566884863 46-47 22.138223942407183 26.55641553859557 28.281321486842604 23.024039032154644 48-49 22.533010680965408 26.5332697089653 28.3504052975658 22.583314312503493 50-51 22.530774723765763 26.761764536079983 28.19783419507853 22.50962654507572 52-53 22.2397533236149 27.14170288304133 27.905229131125868 22.713314662217904 54-55 22.13572693588294 26.880632319198877 28.13844564055632 22.845195104361867 56-57 22.666706882733642 26.708544724164973 27.72877299548554 22.895975397615846 58-59 22.12942573617711 27.045526008583142 28.244193797081024 22.580854458158726 60-61 22.438527642220183 27.34233310495814 27.833602369653192 22.385536883168484 62-63 22.567499083870445 26.94771235956937 27.72549619632364 22.759292360236543 64-65 22.063513790642407 26.82326908868981 27.900530315582206 23.212686805085575 66-67 22.324848620932634 26.66079960449649 27.75723608437736 23.257115690193512 68-69 21.93698185587622 26.807382730457952 27.993657312680202 23.26197810098563 70-71 22.09112044253745 26.55610042680852 28.0390101115257 23.31376901912833 72-73 22.385524211286274 26.855818180721315 27.85932617420149 22.899331433790916 74-75 22.63113755103714 26.891846995122076 27.555691398827214 22.92132405501357 76-77 22.398606345372638 26.688158699183468 27.54056958604553 23.37266536939836 78-79 22.152077366921134 26.751181855813037 27.68840733212798 23.408333445137853 80-81 22.06732617400196 27.03842210611276 27.717107320915474 23.17714439896981 82-83 22.722989265497084 26.675972118110302 28.111473400071635 22.489565216320976 84-85 22.823094002619115 26.85507970579258 27.434608264983606 22.8872180266047 86-87 22.121389170709143 27.21822832696248 27.74554229964426 22.91484020268412 88-89 22.527350253347254 26.480369926623702 27.759414762059382 23.23286505796966 90-91 22.7747458278446 26.408055519928197 27.747199792029214 23.06999886019799 92-93 22.798290663937085 26.610841714338324 27.729679417019803 22.861188204704785 94-95 22.190665680950445 26.596231284870264 27.974264534821458 23.23883849935783 96-97 22.304433825827463 26.53600425510596 27.765428051867293 23.394133867199287 98-99 22.10954225761279 26.813103296824497 27.44887302528307 23.628481420279638 100 22.542920689724788 26.580742401017876 27.09957718190534 23.776759727352005 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1335.0 1 968.5 2 1844.0 3 2723.5 4 1700.0 5 1153.0 6 1625.5 7 2723.0 8 4166.0 9 4924.0 10 4342.0 11 3629.5 12 3749.0 13 4139.5 14 4591.5 15 5010.0 16 5489.5 17 6085.0 18 6546.0 19 6901.0 20 7419.0 21 8121.0 22 8902.5 23 10079.5 24 11553.0 25 13318.0 26 15982.0 27 19350.0 28 23198.5 29 28869.5 30 37057.5 31 45405.0 32 55718.5 33 67571.0 34 78611.0 35 89264.0 36 99372.0 37 113412.5 38 121354.0 39 120886.0 40 118146.5 41 114841.5 42 115730.5 43 120819.5 44 128828.5 45 141427.5 46 153624.0 47 161629.0 48 168253.0 49 170672.0 50 170234.5 51 172361.0 52 170436.0 53 162859.5 54 154170.5 55 138410.0 56 123388.0 57 106665.5 58 88530.5 59 76746.5 60 62327.5 61 47410.0 62 37069.5 63 29759.5 64 22270.5 65 14736.5 66 10152.0 67 7520.0 68 5870.5 69 4460.0 70 3452.0 71 2760.0 72 2201.5 73 1777.0 74 1422.0 75 1074.0 76 753.5 77 465.0 78 312.0 79 223.0 80 157.0 81 119.0 82 92.5 83 81.5 84 67.5 85 48.5 86 36.0 87 29.5 88 22.5 89 18.5 90 13.5 91 7.5 92 8.0 93 6.5 94 5.0 95 5.0 96 5.5 97 3.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056486189803442825 2 0.1318503349791038 3 0.016170556296671867 4 0.015924429107985843 5 0.01919792071750998 6 0.051662096905196735 7 0.05680615514873466 8 0.048585507046621415 9 0.039798766410530304 10-11 0.038925014890694914 12-13 0.039712621894490194 14-15 0.017881140258039746 16-17 0.016859712424992738 18-19 0.016638197955175318 20-21 0.020416250301505807 22-23 0.025375713153529218 24-25 0.02432967260161361 26-27 0.019222533436378583 28-29 0.017684238507090923 30-31 0.015604463762694011 32-33 0.022582169561942828 34-35 0.02296366670440617 36-37 0.02298827942327477 38-39 0.02100695555435227 40-41 0.024563493430865335 42-43 0.03178732641880018 44-45 0.04118938502660635 46-47 0.04622268603523557 48-49 0.03945418834636987 50-51 0.027677002367743557 52-53 0.039048078485037926 54-55 0.03561460420286787 56-57 0.03802665065199092 58-59 0.04240771461060217 60-61 0.022422186889296914 62-63 0.024969603292197277 64-65 0.025080360527105987 66-67 0.031553505589548454 68-69 0.03864196862370598 70-71 0.04021718263129655 72-73 0.04397062225875844 74-75 0.026323302829970415 76-77 0.020047059518476767 78-79 0.019456354265630305 80-81 0.02383741822424156 82-83 0.019813238689225045 84-85 0.012306359434301268 86-87 0.013770816206983122 88-89 0.01794267205521125 90-91 0.020453169379808712 92-93 0.019148695279772775 94-95 0.022619088640245734 96-97 0.02452657435256243 98-99 0.026877089004513972 100 0.028206175823418507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4062940.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.78332655750948 #Duplication Level Percentage of deduplicated Percentage of total 1 83.44928868386543 60.73716829269659 2 11.37446961487946 16.55743472796482 3 2.7164879798260695 5.9314509517569025 4 0.9639742815970966 2.8064501972208835 5 0.4595784643445753 1.6724824724594978 6 0.2606795913223583 1.1383876693255992 7 0.1601702217206984 0.8160405086600405 8 0.11704159344447487 0.6814941213184381 9 0.081914748201811 0.5365825081423714 >10 0.38457939136620967 4.890608052472207 >50 0.020005485414350578 0.9918967535643952 >100 0.010801641745812402 1.4177082987845426 >500 5.975124697663899E-4 0.2824606968643956 >1k 2.987562196090983E-4 0.4926481822609223 >5k 3.7344527451136824E-5 0.22010841268683723 >10k+ 7.468905490227365E-5 0.8270781538216562 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16830 0.4142320585585807 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13599 0.3347083638941259 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8098 0.19931379739794333 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4503 0.11083107306531723 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 2.4612718868602538E-5 3 0.0 0.0 0.0 0.0 2.4612718868602538E-5 4 0.0 0.0 0.0 0.0 2.4612718868602538E-5 5 0.0 0.0 0.0 0.0 2.4612718868602538E-5 6 0.0 0.0 0.0 0.0 7.383815660580762E-5 7 0.0 0.0 0.0 0.0 7.383815660580762E-5 8 2.4612718868602538E-5 0.0 0.0 0.0 7.383815660580762E-5 9 2.4612718868602538E-5 0.0 0.0 0.0 7.383815660580762E-5 10-11 2.4612718868602538E-5 0.0 0.0 0.0 7.383815660580762E-5 12-13 2.4612718868602538E-5 0.0 0.0 0.0 7.383815660580762E-5 14-15 2.4612718868602538E-5 0.0 0.0 0.0 8.614451604010889E-5 16-17 2.4612718868602538E-5 0.0 0.0 1.2306359434301269E-5 9.845087547441015E-5 18-19 2.4612718868602538E-5 0.0 0.0 9.845087547441015E-5 9.845087547441015E-5 20-21 2.4612718868602538E-5 0.0 0.0 1.8459539151451904E-4 9.845087547441015E-5 22-23 2.4612718868602538E-5 0.0 0.0 5.291734556749546E-4 9.845087547441015E-5 24-25 2.4612718868602538E-5 0.0 0.0 0.0013660058972074407 1.3536995377731395E-4 26-27 2.4612718868602538E-5 0.0 0.0 0.002928913545363702 1.4767631321161524E-4 28-29 2.4612718868602538E-5 0.0 0.0 0.0063746941869680575 1.4767631321161524E-4 30-31 2.4612718868602538E-5 1.2306359434301269E-5 0.0 0.018951793528823955 1.599826726459165E-4 32-33 2.4612718868602538E-5 2.4612718868602538E-5 0.0 0.047157969352242465 1.969017509488203E-4 34-35 2.4612718868602538E-5 4.9225437737205076E-5 0.0 0.0881996780656372 1.969017509488203E-4 36-37 2.4612718868602538E-5 4.9225437737205076E-5 0.0 0.15445711725991523 1.969017509488203E-4 38-39 2.4612718868602538E-5 4.9225437737205076E-5 0.0 0.25450781946078455 1.969017509488203E-4 40-41 2.4612718868602538E-5 4.9225437737205076E-5 0.0 0.3697937946413189 1.969017509488203E-4 42-43 2.4612718868602538E-5 4.9225437737205076E-5 0.0 0.48367684484634277 1.969017509488203E-4 44-45 2.4612718868602538E-5 4.9225437737205076E-5 0.0 0.61150300029043 1.969017509488203E-4 46-47 2.4612718868602538E-5 4.9225437737205076E-5 0.0 0.7586132209680674 1.969017509488203E-4 48-49 2.4612718868602538E-5 4.9225437737205076E-5 0.0 0.9236538073414817 2.4612718868602536E-4 50-51 2.4612718868602538E-5 4.9225437737205076E-5 0.0 1.0913156482744022 2.4612718868602536E-4 52-53 2.4612718868602538E-5 4.9225437737205076E-5 0.0 1.2604296396205703 2.5843354812032666E-4 54-55 2.4612718868602538E-5 4.9225437737205076E-5 0.0 1.460740252132692 2.8304626698892924E-4 56-57 2.4612718868602538E-5 4.9225437737205076E-5 0.0 1.678538201400956 2.953526264232305E-4 58-59 2.4612718868602538E-5 4.9225437737205076E-5 0.0 1.9185368230886994 2.953526264232305E-4 60-61 2.4612718868602538E-5 4.9225437737205076E-5 0.0 2.161587421916149 2.953526264232305E-4 62-63 4.9225437737205076E-5 4.9225437737205076E-5 0.0 2.423048334457314 2.953526264232305E-4 64-65 4.9225437737205076E-5 4.9225437737205076E-5 0.0 2.6819987496738813 2.953526264232305E-4 66-67 4.9225437737205076E-5 4.9225437737205076E-5 0.0 2.9611562070815713 2.953526264232305E-4 68-69 4.9225437737205076E-5 4.9225437737205076E-5 0.0 3.2696520253806356 2.953526264232305E-4 70-71 4.9225437737205076E-5 4.9225437737205076E-5 0.0 3.5948106543537435 3.19965345291833E-4 72-73 7.383815660580762E-5 4.9225437737205076E-5 0.0 3.91515749678804 3.19965345291833E-4 74-75 7.383815660580762E-5 4.9225437737205076E-5 0.0 4.237781015717682 3.19965345291833E-4 76-77 7.383815660580762E-5 4.9225437737205076E-5 0.0 4.60090476354561 3.19965345291833E-4 78-79 7.383815660580762E-5 4.9225437737205076E-5 0.0 4.995212826180056 3.4457806416043554E-4 80-81 7.383815660580762E-5 4.9225437737205076E-5 0.0 5.392339045124959 3.691907830290381E-4 82-83 1.1075723490871143E-4 4.9225437737205076E-5 0.0 5.793600200839785 3.938035018976406E-4 84-85 1.4767631321161524E-4 7.383815660580762E-5 0.0 6.209752051470118 3.938035018976406E-4 86-87 1.4767631321161524E-4 7.383815660580762E-5 0.0 6.641631429457487 3.938035018976406E-4 88 1.4767631321161524E-4 7.383815660580762E-5 0.0 6.986098736383013 3.938035018976406E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7795 0.0 51.435226 1 TACCTGG 3685 0.0 44.567654 2 GTATCAA 13865 0.0 40.49093 1 ACCTGGG 4350 0.0 37.066982 3 GTACCTG 4545 0.0 36.238594 1 TATCACG 370 0.0 34.339493 2 ATCAACG 16705 0.0 32.783993 3 CAACGCA 16820 0.0 32.559444 5 TATCAAC 17325 0.0 32.07809 2 GTGGTAT 3085 0.0 30.2028 1 AACGCAG 18475 0.0 30.126938 6 TCAACGC 18240 0.0 30.076206 4 GTACTAG 2300 0.0 28.848846 1 GTACATG 18195 0.0 28.760069 1 GTACACG 760 0.0 28.482718 1 TACATGG 18135 0.0 28.050459 2 CCTGGGG 4170 0.0 27.393484 4 ACATGGG 18070 0.0 27.003593 3 ACGCAGA 20490 0.0 26.70538 7 CGCAGAG 20960 0.0 26.060087 8 >>END_MODULE