FastQCFastQC Report
Mon 6 Jun 2016
ERR1211262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062940
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156390.3849183103860751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134660.3314348722846018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80500.19813238689225043No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80250.051.4465681
TACCTGG35350.047.9954532
GTATCAA139100.039.7883831
GTACCTG45400.039.4617231
ACCTGGG43750.038.0311623
GTGGTAT29850.034.8140751
ATCAACG169200.031.7234293
CAACGCA170200.031.4445175
TATCAAC176100.031.2254582
GTACTAG22900.030.800851
CCTGGGG39500.029.3909914
TCAACGC185450.029.0197094
AACGCAG188950.028.9458876
TGGTATC35150.028.346072
GTACATG179300.028.1925961
TACATGG179550.027.641452
TATCACG3500.026.8561822
ACATGGG178250.026.5266763
ACGCAGA207050.026.0069967
CGCAGAG210400.025.5705768
Produced by FastQC (version 0.11.5)