FastQCFastQC Report
Mon 6 Jun 2016
ERR1211261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11108995
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375740.3382304159827239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300160.2701954587251142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182660.1644253148012039No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA223650.047.030341
GTATCAA394550.040.0780641
GTACTAG119650.037.0516621
GGGCACC120450.035.11468594
GCATCGT117650.034.6421978
CAACGCA438650.034.091595
ATCAACG447550.033.581713
TAGGCAT145000.032.875455
TACCTGG50350.032.2469832
TATCAAC497750.031.617282
CTGTGCG147200.031.5511829
AACGCAG504350.030.591896
GTGGTAT71500.030.211771
CTAGGCA162800.029.9162464
ACTGTGC163750.029.5114178
TAGTACT158750.029.4653134
TCTAACG57700.028.5470832
TCAACGC537150.028.2951414
ACGCAGA534950.027.9287977
CGCAGAG536050.027.8167328
Produced by FastQC (version 0.11.5)