##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211261_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11108995 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.531603893961606 31.0 31.0 34.0 30.0 34.0 2 31.546207825280323 31.0 31.0 34.0 28.0 34.0 3 31.610320735584093 33.0 31.0 34.0 28.0 34.0 4 35.21739410270686 37.0 35.0 37.0 32.0 37.0 5 35.281771933464725 37.0 35.0 37.0 33.0 37.0 6 35.34220728337712 37.0 35.0 37.0 32.0 37.0 7 35.260595580428294 37.0 35.0 37.0 32.0 37.0 8 35.269607736793475 37.0 35.0 37.0 32.0 37.0 9 36.837120909677246 39.0 37.0 39.0 32.0 39.0 10-11 36.79400584841383 39.0 37.0 39.0 32.0 39.0 12-13 36.681670889220854 39.0 37.0 39.0 32.0 39.0 14-15 37.86761016635619 40.0 37.5 41.0 32.0 41.0 16-17 37.85443300676614 40.0 37.5 41.0 32.0 41.0 18-19 37.88027638863822 40.0 38.0 41.0 32.0 41.0 20-21 37.85492985639115 40.0 37.5 41.0 32.0 41.0 22-23 37.73818954819946 40.0 37.0 41.0 32.0 41.0 24-25 37.638625186166706 40.0 37.0 41.0 32.0 41.0 26-27 37.486218060229575 40.0 37.0 41.0 31.5 41.0 28-29 37.3743186489867 40.0 36.5 41.0 31.0 41.0 30-31 37.22096940362292 40.0 36.0 41.0 31.0 41.0 32-33 37.0443356487243 39.0 36.0 41.0 31.0 41.0 34-35 36.903929923453916 39.0 35.5 41.0 30.0 41.0 36-37 36.798162300010034 39.0 35.0 41.0 30.0 41.0 38-39 36.64596239353785 39.0 35.0 41.0 30.0 41.0 40-41 36.47258878953497 39.0 35.0 40.5 30.0 41.0 42-43 36.252558174704376 39.0 35.0 40.0 29.0 41.0 44-45 35.989859568754866 38.0 35.0 40.0 28.0 41.0 46-47 35.72783379594644 38.0 34.5 40.0 27.0 41.0 48-49 35.484231966978115 38.0 34.0 40.0 26.5 41.0 50-51 34.7697362362662 37.5 33.5 39.5 25.5 40.5 52-53 34.85372421177613 38.0 33.5 39.5 26.0 40.5 54-55 35.30985984780801 38.0 34.0 40.0 27.0 41.0 56-57 35.240348069289794 38.0 34.0 40.0 26.0 41.0 58-59 34.99770294252541 37.0 34.0 40.0 26.0 41.0 60-61 34.7707591010708 37.0 34.0 40.0 26.0 41.0 62-63 34.46452532384792 36.0 33.5 40.0 26.0 41.0 64-65 34.21709529079813 36.0 33.0 39.0 26.0 41.0 66-67 34.05767690956743 35.5 33.0 39.0 26.0 41.0 68-69 33.77801835359544 35.0 33.0 39.0 25.0 41.0 70-71 33.52446598454675 35.0 33.0 38.5 25.0 41.0 72-73 33.22649218043576 35.0 32.5 37.5 24.5 40.0 74-75 32.91394095505489 35.0 32.0 37.0 24.0 39.5 76-77 32.61653767960108 35.0 32.0 36.5 23.5 39.0 78-79 32.30354329982145 35.0 32.0 36.0 23.0 39.0 80-81 32.03377470239207 35.0 31.0 36.0 23.0 38.5 82-83 31.75169765581855 35.0 31.0 35.0 21.5 37.0 84-85 31.520464632489258 35.0 31.0 35.0 20.5 37.0 86-87 31.30728306205917 34.5 31.0 35.0 20.0 36.5 88-89 31.114900717841714 34.0 31.0 35.0 20.0 36.0 90-91 30.888523984392826 34.0 30.5 35.0 19.5 36.0 92-93 30.687419429030257 34.0 30.0 35.0 19.0 35.5 94-95 30.535556006641464 34.0 30.0 35.0 19.0 35.0 96-97 30.35491194297954 34.0 29.5 35.0 18.0 35.0 98-99 30.12641607094071 34.0 29.0 35.0 18.0 35.0 100 28.96559292717298 33.0 27.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5130435156705389 1101 2 -0.49052489258753695 1101 3 -0.5238308566337224 1101 4 -0.4061670634779304 1101 5 -0.3388816141426432 1101 6 -0.33411295838049426 1101 7 -0.3674407039159675 1101 8 -0.3368119487187897 1101 9 -0.5843691371556474 1101 10-11 -0.5720664086120806 1101 12-13 -0.5670124066562536 1101 14-15 -0.7470808348519498 1101 16-17 -0.7055554948360836 1101 18-19 -0.7095023096453303 1101 20-21 -0.8452052716093164 1101 22-23 -0.775433856705483 1101 24-25 -0.9111469356949939 1101 26-27 -0.7907685915038343 1101 28-29 -0.8542074268127635 1101 30-31 -0.9141558113337496 1101 32-33 -1.0049271441806553 1101 34-35 -0.9922021552925386 1101 36-37 -0.9659477559730547 1101 38-39 -1.0077427499749092 1101 40-41 -1.0648816994119557 1101 42-43 -1.0926486167996003 1101 44-45 -1.1611665381405771 1101 46-47 -1.2724787000464914 1101 48-49 -1.250940915751194 1101 50-51 -1.325591900518674 1101 52-53 -1.1415497048490053 1101 54-55 -0.982047932441823 1101 56-57 -0.8995655158634506 1101 58-59 -0.966116728552656 1101 60-61 -0.9022343181445152 1101 62-63 -0.9971191888809798 1101 64-65 -0.8906647994766033 1101 66-67 -0.9591492599024392 1101 68-69 -0.9164360692995714 1101 70-71 -0.9581119219289107 1101 72-73 -0.9849827226297165 1101 74-75 -1.0405358471872823 1101 76-77 -1.0387747111945949 1101 78-79 -1.1710166375361268 1101 80-81 -1.1854537779607952 1101 82-83 -1.098513360707127 1101 84-85 -1.263575457333939 1101 86-87 -1.2697277672268257 1101 88-89 -1.3526078096164333 1101 90-91 -1.3445899260384806 1101 92-93 -1.5077206031145707 1101 94-95 -1.5483895630038624 1101 96-97 -1.6146923568692628 1101 98-99 -1.6943907113522059 1101 100 -1.8517560298733926 1102 1 -0.46342484763505 1102 2 -0.431542974211105 1102 3 -0.43296576586888946 1102 4 -0.2491492977911136 1102 5 -0.3537213797601595 1102 6 -0.30703637942942663 1102 7 -0.37237832546145455 1102 8 -0.32967836640226267 1102 9 -0.47393589562808813 1102 10-11 -0.5512519242988816 1102 12-13 -0.5942045203611102 1102 14-15 -0.7032183972958066 1102 16-17 -0.7438723476927649 1102 18-19 -0.6660437109132076 1102 20-21 -0.7043116386973338 1102 22-23 -0.71072942492453 1102 24-25 -0.7605785353493033 1102 26-27 -0.7510557947847403 1102 28-29 -0.8626644951648714 1102 30-31 -0.8671569541196433 1102 32-33 -0.9569451882076692 1102 34-35 -0.9707547809579253 1102 36-37 -0.9040812794786817 1102 38-39 -1.1088322662255337 1102 40-41 -1.098575738005266 1102 42-43 -1.0667768560843243 1102 44-45 -1.1878050995841392 1102 46-47 -1.1974856398906724 1102 48-49 -1.1549676783282194 1102 50-51 -1.128428609461885 1102 52-53 -1.0918547535276986 1102 54-55 -0.8412234180600251 1102 56-57 -0.8713193138812514 1102 58-59 -0.8448867208235669 1102 60-61 -0.870964903571938 1102 62-63 -0.985752723936649 1102 64-65 -0.9649430095208729 1102 66-67 -1.0172927840122767 1102 68-69 -1.0815524543954425 1102 70-71 -1.0575368724542784 1102 72-73 -1.0295380593325731 1102 74-75 -1.0396011338549016 1102 76-77 -1.169729094555752 1102 78-79 -1.150946840932292 1102 80-81 -1.2279915263524153 1102 82-83 -1.268454842872412 1102 84-85 -1.2277320313365614 1102 86-87 -1.203221318377114 1102 88-89 -1.26960121336624 1102 90-91 -1.3258445386875266 1102 92-93 -1.2402808512942407 1102 94-95 -1.277240058842274 1102 96-97 -1.3390835804983432 1102 98-99 -1.492744650241189 1102 100 -1.5268565093099653 1103 1 -0.5758105068967367 1103 2 -0.5027730347327832 1103 3 -0.5951582036247949 1103 4 -0.520906859279016 1103 5 -0.49771991084279676 1103 6 -0.48830578858193974 1103 7 -0.4772087324547414 1103 8 -0.5079563836591703 1103 9 -0.6986793121647068 1103 10-11 -0.6844229899445438 1103 12-13 -0.7289133774316028 1103 14-15 -0.8504346737807182 1103 16-17 -0.8378974851360326 1103 18-19 -0.8105060345803778 1103 20-21 -0.8041924321850971 1103 22-23 -0.8868443305122824 1103 24-25 -0.9212508987986254 1103 26-27 -0.9295424236855041 1103 28-29 -0.9905442642666742 1103 30-31 -1.0361625789780575 1103 32-33 -1.0498018371630238 1103 34-35 -1.1248574093151902 1103 36-37 -1.0290796314897435 1103 38-39 -1.0384126500380972 1103 40-41 -1.0839201823406128 1103 42-43 -1.1795851428496817 1103 44-45 -1.2072404120693676 1103 46-47 -1.2628484243137663 1103 48-49 -1.3207404536936096 1103 50-51 -1.396716990936028 1103 52-53 -1.2612741027904164 1103 54-55 -1.0514045709420046 1103 56-57 -0.9808563030274513 1103 58-59 -0.9265495658055514 1103 60-61 -1.0012200235419684 1103 62-63 -1.0206929299493126 1103 64-65 -1.0155025329410847 1103 66-67 -1.064587220702542 1103 68-69 -1.0141307131831852 1103 70-71 -0.9478298286665137 1103 72-73 -0.9989741745319307 1103 74-75 -1.0195998683181742 1103 76-77 -1.0635421452265525 1103 78-79 -1.1561609244932711 1103 80-81 -1.15507723104119 1103 82-83 -1.0910358584041688 1103 84-85 -1.2109152742842184 1103 86-87 -1.20796644268556 1103 88-89 -1.2498969965970765 1103 90-91 -1.3807853520311326 1103 92-93 -1.4620973108177715 1103 94-95 -1.5072067193828467 1103 96-97 -1.5453622411538888 1103 98-99 -1.586693836675078 1103 100 -1.5482152178852644 1104 1 -0.533280801594394 1104 2 -0.5542010353968756 1104 3 -0.5949574912104545 1104 4 -0.41659510000742017 1104 5 -0.3998448804968291 1104 6 -0.43701231286343756 1104 7 -0.48815786557726426 1104 8 -0.44446059969969554 1104 9 -0.55375727454323 1104 10-11 -0.6221491240352464 1104 12-13 -0.6199855315102454 1104 14-15 -0.758940448046836 1104 16-17 -0.869548082018774 1104 18-19 -0.908795703145536 1104 20-21 -0.8239669291169562 1104 22-23 -0.7823654828317714 1104 24-25 -0.8281192249758718 1104 26-27 -0.8129170667407735 1104 28-29 -0.9457347260594915 1104 30-31 -0.9128281025088754 1104 32-33 -1.000548593784238 1104 34-35 -1.0908028334327255 1104 36-37 -1.0254673961504395 1104 38-39 -0.9941936795236757 1104 40-41 -1.041635245335847 1104 42-43 -1.0110344874057375 1104 44-45 -1.1329067506750121 1104 46-47 -1.1954693256318691 1104 48-49 -1.1781754950075083 1104 50-51 -1.21863759605435 1104 52-53 -1.1125619479944646 1104 54-55 -0.9473370786208122 1104 56-57 -0.8730090241760351 1104 58-59 -0.9580716539088314 1104 60-61 -0.9374698781994866 1104 62-63 -1.0305254857767352 1104 64-65 -0.9410998447175203 1104 66-67 -1.0002986043879716 1104 68-69 -1.0102202051970224 1104 70-71 -0.9817533263652294 1104 72-73 -0.9494845989859542 1104 74-75 -0.9690311526134536 1104 76-77 -0.8964315179583195 1104 78-79 -1.0060146275302948 1104 80-81 -1.1099514343571393 1104 82-83 -1.1555529588743383 1104 84-85 -1.1229250362850713 1104 86-87 -1.0566443130475918 1104 88-89 -1.2403588761823556 1104 90-91 -1.2640201577889911 1104 92-93 -1.3080287338512413 1104 94-95 -1.3324938370550043 1104 96-97 -1.458349104700872 1104 98-99 -1.3978636541480718 1104 100 -1.407599569335808 1105 1 -0.6405519862249314 1105 2 -0.6079113123604785 1105 3 -0.6213592560524503 1105 4 -0.45617169664208745 1105 5 -0.3744205055635774 1105 6 -0.41288835563022985 1105 7 -0.39283982170689313 1105 8 -0.4288141287903642 1105 9 -0.6104028732663949 1105 10-11 -0.5966667702302573 1105 12-13 -0.6035361820899183 1105 14-15 -0.7906065720693434 1105 16-17 -0.7991927143656099 1105 18-19 -0.8437460790162419 1105 20-21 -0.8108283457074705 1105 22-23 -0.8109005623668466 1105 24-25 -0.8733527572704887 1105 26-27 -0.8815156022690118 1105 28-29 -0.870932191676367 1105 30-31 -0.9621417750652697 1105 32-33 -0.9697886051760563 1105 34-35 -1.0055182493231243 1105 36-37 -0.9801931284604919 1105 38-39 -0.9702565451595433 1105 40-41 -0.9939996596954828 1105 42-43 -1.0666254157796757 1105 44-45 -1.1108806199479488 1105 46-47 -1.1395097762317192 1105 48-49 -1.048955886541961 1105 50-51 -1.108039254839703 1105 52-53 -0.9819949009082833 1105 54-55 -0.8825391350815153 1105 56-57 -0.8712162217884938 1105 58-59 -0.7387543249937849 1105 60-61 -0.7994306023810012 1105 62-63 -0.8738382245896119 1105 64-65 -0.8249819408715879 1105 66-67 -0.8307144836643587 1105 68-69 -0.9112794552652517 1105 70-71 -0.9107516250088921 1105 72-73 -0.8602666295777297 1105 74-75 -0.8996021860947252 1105 76-77 -0.8887785108443609 1105 78-79 -0.9509924557570884 1105 80-81 -1.0101105378743647 1105 82-83 -1.0561316907334586 1105 84-85 -1.0105917603063865 1105 86-87 -1.083581460019861 1105 88-89 -1.0696911791098138 1105 90-91 -1.1027082518545654 1105 92-93 -1.1720732116371941 1105 94-95 -1.186305257205472 1105 96-97 -1.1834151676110096 1105 98-99 -1.143579688578658 1105 100 -1.2235339135942098 1106 1 -0.5538360020327104 1106 2 -0.5573659601443026 1106 3 -0.6492319036759007 1106 4 -0.4197334601405771 1106 5 -0.4672081765013445 1106 6 -0.48754983123038187 1106 7 -0.4892557312765362 1106 8 -0.5005808377850514 1106 9 -0.6675870617772688 1106 10-11 -0.5885747721703183 1106 12-13 -0.6492107579320745 1106 14-15 -0.8629010660061809 1106 16-17 -0.8454184882083311 1106 18-19 -0.8517533823944277 1106 20-21 -0.7934250940001917 1106 22-23 -0.8254136477980225 1106 24-25 -0.8010565241847445 1106 26-27 -0.8616662469733782 1106 28-29 -0.9400029954578883 1106 30-31 -0.9254568786272941 1106 32-33 -0.9508723500206884 1106 34-35 -0.9572282740317561 1106 36-37 -1.0431272611689977 1106 38-39 -0.9366891759914182 1106 40-41 -0.9859666056931431 1106 42-43 -0.9742300444740053 1106 44-45 -1.0225702102179355 1106 46-47 -0.9044060354383632 1106 48-49 -0.9027527440756771 1106 50-51 -0.960012605393473 1106 52-53 -0.8887696002636645 1106 54-55 -0.7057379303487537 1106 56-57 -0.6908386536826754 1106 58-59 -0.6837146005886652 1106 60-61 -0.6924023874763492 1106 62-63 -0.7481409380808017 1106 64-65 -0.7392517362010693 1106 66-67 -0.8128261029144639 1106 68-69 -0.8726767263947508 1106 70-71 -0.7532663056697331 1106 72-73 -0.7014879444670612 1106 74-75 -0.6988642521228385 1106 76-77 -0.7883461210248726 1106 78-79 -0.8019454682173226 1106 80-81 -0.8037116555075521 1106 82-83 -0.695081104913303 1106 84-85 -0.7538387760016612 1106 86-87 -0.785640212135263 1106 88-89 -0.7373898654651931 1106 90-91 -0.8980373915825055 1106 92-93 -0.8863478578830879 1106 94-95 -0.9984587551612805 1106 96-97 -1.0031524020336207 1106 98-99 -0.9479734407179983 1106 100 -1.147454377115917 1107 1 -0.518096416343429 1107 2 -0.537051442569183 1107 3 -0.547322612126262 1107 4 -0.3845019335633637 1107 5 -0.3852142614966425 1107 6 -0.40052691195246126 1107 7 -0.48456542056302254 1107 8 -0.42911889956732097 1107 9 -0.6261041028578234 1107 10-11 -0.6110376716905606 1107 12-13 -0.6860196255834978 1107 14-15 -0.8235948355164453 1107 16-17 -0.8377727467288665 1107 18-19 -0.8144787125427442 1107 20-21 -0.7683668767034746 1107 22-23 -0.8912088797015585 1107 24-25 -0.8771071199940579 1107 26-27 -0.8723526651701619 1107 28-29 -0.9047106084150585 1107 30-31 -0.905787665958222 1107 32-33 -1.0087570542425794 1107 34-35 -0.9717942559374606 1107 36-37 -1.0053289322524606 1107 38-39 -0.9047278763392654 1107 40-41 -1.0117621507220775 1107 42-43 -0.9573749806880301 1107 44-45 -0.9301730602748677 1107 46-47 -0.8923745108435241 1107 48-49 -0.9374625451561869 1107 50-51 -0.9192333526473604 1107 52-53 -0.7982692619080112 1107 54-55 -0.7461240304942223 1107 56-57 -0.7668628553480517 1107 58-59 -0.7792177743436852 1107 60-61 -0.8380232535827261 1107 62-63 -0.8310564141951104 1107 64-65 -0.841700868716039 1107 66-67 -0.7796572242458311 1107 68-69 -0.7676111382611381 1107 70-71 -0.8369974659578077 1107 72-73 -0.8326267980886612 1107 74-75 -0.7615212841385244 1107 76-77 -0.805394222493721 1107 78-79 -0.7493837457182195 1107 80-81 -0.777297875526731 1107 82-83 -0.8522945380983522 1107 84-85 -0.9089594877229423 1107 86-87 -0.8648396142985142 1107 88-89 -0.9059219748611476 1107 90-91 -0.9813801862590381 1107 92-93 -0.9873316085517665 1107 94-95 -1.0509917181806436 1107 96-97 -1.0734629848914103 1107 98-99 -1.0267568065953476 1107 100 -1.0313140990365284 1108 1 -0.6089475415816885 1108 2 -0.5586817742636612 1108 3 -0.5841069969738761 1108 4 -0.3905391593608627 1108 5 -0.4334424641762027 1108 6 -0.42485302712966444 1108 7 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50-51 1.3956848602684744 2311 52-53 1.326188578400071 2311 54-55 1.2036491946989187 2311 56-57 1.2018481102346712 2311 58-59 1.1791551186682128 2311 60-61 1.1500764867521056 2311 62-63 1.1244309034161617 2311 64-65 1.1122168481130146 2311 66-67 1.1193007233956394 2311 68-69 1.109831836972468 2311 70-71 1.0722176039865872 2311 72-73 1.0875155675296853 2311 74-75 1.150599261105505 2311 76-77 1.1571848728514667 2311 78-79 1.1835724740237268 2311 80-81 1.2213481809676132 2311 82-83 1.2218559673290663 2311 84-85 1.2221888006816393 2311 86-87 1.1826160920680557 2311 88-89 1.2360873362505735 2311 90-91 1.3149923713059906 2311 92-93 1.3952055307227944 2311 94-95 1.3802759816239494 2311 96-97 1.4368858030096447 2311 98-99 1.494263519755794 2311 100 1.6424369917060488 2312 1 0.6451425534813708 2312 2 0.6334300376937634 2312 3 0.6812232223782573 2312 4 0.41917715373990205 2312 5 0.46987935459939933 2312 6 0.5433617512424931 2312 7 0.45408581221460764 2312 8 0.5485149009763006 2312 9 0.7146731369895818 2312 10-11 0.6980578763535163 2312 12-13 0.8455834657240828 2312 14-15 1.0246575573359493 2312 16-17 1.0280224870443604 2312 18-19 1.0149992031467505 2312 20-21 1.038933942637037 2312 22-23 1.0866742669455434 2312 24-25 1.0478846578725651 2312 26-27 1.0912438318612985 2312 28-29 1.1144635615815872 2312 30-31 1.13607817659976 2312 32-33 1.1664047689050818 2312 34-35 1.1307662189114538 2312 36-37 1.1859705486594407 2312 38-39 1.1971535796297985 2312 40-41 1.2564186431134274 2312 42-43 1.258020342703169 2312 44-45 1.2726944169350105 2312 46-47 1.3395307162995422 2312 48-49 1.3325500201947236 2312 50-51 1.312396243790424 2312 52-53 1.1940432072925162 2312 54-55 1.0912755039166697 2312 56-57 1.0435417969907022 2312 58-59 1.0564618322145662 2312 60-61 1.0514369280468472 2312 62-63 1.0457537216956823 2312 64-65 1.0568542619547898 2312 66-67 1.0368524738781986 2312 68-69 1.0235903624763765 2312 70-71 1.0376654533948368 2312 72-73 1.0586225543326506 2312 74-75 1.100140681100953 2312 76-77 1.1002712356772903 2312 78-79 1.0912876553952984 2312 80-81 1.1344838783583278 2312 82-83 1.1603772624695843 2312 84-85 1.2082611343769578 2312 86-87 1.1773497344894501 2312 88-89 1.0579418085575476 2312 90-91 1.1383151221600656 2312 92-93 1.2627411807224966 2312 94-95 1.3512781836734895 2312 96-97 1.3829410229083834 2312 98-99 1.0157960862485886 2312 100 1.2593903485565043 2313 1 0.6194723580289008 2313 2 0.6144261367638322 2313 3 0.6470502651500496 2313 4 0.5152697958653576 2313 5 0.29554130710521775 2313 6 0.4669947593474646 2313 7 0.5672168490986635 2313 8 0.5385776013180816 2313 9 0.69530447538353 2313 10-11 0.7147647016687984 2313 12-13 0.7849848467536731 2313 14-15 0.9741567308925454 2313 16-17 0.9779334993376381 2313 18-19 0.9638594569014955 2313 20-21 0.9773048296100981 2313 22-23 1.0038239894878629 2313 24-25 0.9807544751827137 2313 26-27 0.9840265605884326 2313 28-29 1.0817672027512657 2313 30-31 1.077415712875812 2313 32-33 1.1299931750337393 2313 34-35 1.1578554081429004 2313 36-37 1.1875001227336313 2313 38-39 1.23899065996072 2313 40-41 1.2341808943129067 2313 42-43 1.3005950136047844 2313 44-45 1.2586521824171015 2313 46-47 1.291148482588092 2313 48-49 1.2856718956584743 2313 50-51 1.3324851117161103 2313 52-53 1.2471927375451415 2313 54-55 1.1386154826285662 2313 56-57 1.0525260030843029 2313 58-59 1.0451216200668156 2313 60-61 0.9825941508934264 2313 62-63 1.061107901409109 2313 64-65 1.0894878957556386 2313 66-67 1.0675966761790363 2313 68-69 1.041042687567959 2313 70-71 1.0636205402621428 2313 72-73 1.089319232822895 2313 74-75 1.040918812522328 2313 76-77 0.8608376714957302 2313 78-79 1.0113953522593064 2313 80-81 1.0720368140314243 2313 82-83 1.0941468547834141 2313 84-85 1.1543798425924656 2313 86-87 1.1918227464054603 2313 88-89 1.2329699113823374 2313 90-91 1.2211894472602438 2313 92-93 1.2958979879014585 2313 94-95 1.3974791110529914 2313 96-97 1.443076701901795 2313 98-99 1.4873420675812206 2313 100 1.5169286215682014 2314 1 0.5615399043995737 2314 2 0.5460614979061233 2314 3 0.586511726943499 2314 4 0.43953454611082776 2314 5 0.4493514093667983 2314 6 0.46802271679422347 2314 7 0.5072800436898817 2314 8 0.48712871025661286 2314 9 0.6623435864317031 2314 10-11 0.6189298591644388 2314 12-13 0.6730251731450565 2314 14-15 0.843200652327802 2314 16-17 0.8174490119440563 2314 18-19 0.7985998963127088 2314 20-21 0.8255235849419549 2314 22-23 0.8423020095681082 2314 24-25 0.8438739696778939 2314 26-27 0.9386201054474412 2314 28-29 0.9251057515640824 2314 30-31 0.8854118299319182 2314 32-33 0.9190146187564991 2314 34-35 0.9920473467842328 2314 36-37 0.9486555222907427 2314 38-39 0.9358551403733486 2314 40-41 1.0300674824945375 2314 42-43 1.0896416450041926 2314 44-45 1.1131450007646464 2314 46-47 1.1369718846468047 2314 48-49 1.0241021820533263 2314 50-51 1.034571062659694 2314 52-53 1.0017847498628285 2314 54-55 0.939203082173087 2314 56-57 0.8836204568772317 2314 58-59 0.8863220950347497 2314 60-61 0.8797171298387596 2314 62-63 0.8812827921422652 2314 64-65 0.8678291047126478 2314 66-67 0.8922119555202386 2314 68-69 0.9135735199126387 2314 70-71 0.9411321126262138 2314 72-73 0.9791251078439345 2314 74-75 0.9821804467214434 2314 76-77 0.9559389370086251 2314 78-79 0.909483807486481 2314 80-81 0.95337273958663 2314 82-83 0.9858036267430954 2314 84-85 1.0692464523302014 2314 86-87 1.1420298137975387 2314 88-89 1.1595735055331993 2314 90-91 1.1790223566399618 2314 92-93 1.202372320028534 2314 94-95 1.25926710374193 2314 96-97 1.287639848553674 2314 98-99 1.3328964005863533 2314 100 1.4081151145024222 2315 1 0.45438761600444977 2315 2 0.4522843610708769 2315 3 0.5066590053216444 2315 4 0.24517094292009034 2315 5 0.34658671230376115 2315 6 0.36455832638036156 2315 7 0.4078999841765878 2315 8 0.4188260234667922 2315 9 0.5762879781607282 2315 10-11 0.45366042235148996 2315 12-13 0.5206186926829375 2315 14-15 0.7711093354237377 2315 16-17 0.7284115832382057 2315 18-19 0.6251852092011561 2315 20-21 0.7562621685727393 2315 22-23 0.6075813101303496 2315 24-25 0.7068603724721996 2315 26-27 0.7833470431801786 2315 28-29 0.833467395452999 2315 30-31 0.878778505887233 2315 32-33 0.9059184554290809 2315 34-35 0.9071713244919337 2315 36-37 0.9430885845265848 2315 38-39 1.010419411086886 2315 40-41 1.0296912466965793 2315 42-43 1.1136902133792503 2315 44-45 1.1452595211556513 2315 46-47 1.194138278158853 2315 48-49 1.1881043811134475 2315 50-51 1.0580021690197867 2315 52-53 1.0904922226177902 2315 54-55 0.9892475239177756 2315 56-57 0.9055053790334142 2315 58-59 0.9002780969749367 2315 60-61 0.955234130324861 2315 62-63 0.9262234761967889 2315 64-65 0.9399211319002561 2315 66-67 0.9074108728765466 2315 68-69 1.0068300133428707 2315 70-71 0.9668862973981689 2315 72-73 0.6827171214470198 2315 74-75 0.9101610829326034 2315 76-77 0.899277961765037 2315 78-79 0.9352013694820798 2315 80-81 0.6799139530592022 2315 82-83 0.7327987945142702 2315 84-85 1.0016808448741834 2315 86-87 1.1053037343547167 2315 88-89 1.1713846322379275 2315 90-91 1.2083267836578813 2315 92-93 1.2368272441789152 2315 94-95 1.2924310527421419 2315 96-97 1.1800317280373953 2315 98-99 0.6239476262964736 2315 100 1.17327759431317 2316 1 0.38563557755902167 2316 2 0.3601390908126625 2316 3 0.3782181119141974 2316 4 0.2812988518346202 2316 5 0.19746356099062012 2316 6 0.2701284711737628 2316 7 0.2156751363904874 2316 8 0.23976114933694959 2316 9 0.3569354352529359 2316 10-11 0.3485180070449587 2316 12-13 0.1386194188956793 2316 14-15 0.1606244652930826 2316 16-17 0.3729031439108397 2316 18-19 0.4230608382269807 2316 20-21 0.3955967772778948 2316 22-23 0.3383144347665379 2316 24-25 0.4196730949912322 2316 26-27 0.43870946923882315 2316 28-29 0.40727719117418104 2316 30-31 0.3423091740039368 2316 32-33 0.5614619296408634 2316 34-35 0.5912249450471379 2316 36-37 0.5549136122706315 2316 38-39 0.7062560353107017 2316 40-41 0.7529448116149169 2316 42-43 0.6501941344021418 2316 44-45 0.6083468378566863 2316 46-47 0.43038144285136326 2316 48-49 0.28912261928990546 2316 50-51 0.374449945517803 2316 52-53 0.5240373976425161 2316 54-55 0.26512421039084444 2316 56-57 0.12450909916920949 2316 58-59 0.12070604867547985 2316 60-61 0.22618037950457648 2316 62-63 0.3539228423487444 2316 64-65 0.32137227130142065 2316 66-67 0.29603331913885 2316 68-69 0.24554650737142936 2316 70-71 0.10004749674290991 2316 72-73 0.018956904078130776 2316 74-75 0.24269854309155647 2316 76-77 0.3614156910308921 2316 78-79 0.37277514450476446 2316 80-81 0.5651968565944365 2316 82-83 0.613860873340986 2316 84-85 0.690133868030312 2316 86-87 0.7372374693802506 2316 88-89 0.6173541702123266 2316 90-91 0.5387291068974704 2316 92-93 0.6002384565254566 2316 94-95 0.7823690028668757 2316 96-97 0.6944246609705971 2316 98-99 0.5704497991432973 2316 100 0.39968749644440393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 33.0 1 690.0 2 263.0 3 232.0 4 195.0 5 151.0 6 173.0 7 691.0 8 2305.0 9 4591.0 10 7015.0 11 10668.0 12 14409.0 13 18592.0 14 22179.0 15 26920.0 16 33132.0 17 42111.0 18 52999.0 19 66344.0 20 83847.0 21 106245.0 22 116445.0 23 113687.0 24 109226.0 25 111274.0 26 122822.0 27 141611.0 28 168388.0 29 202899.0 30 248282.0 31 311185.0 32 396203.0 33 526306.0 34 763032.0 35 1062418.0 36 1615930.0 37 2200160.0 38 1841670.0 39 563204.0 40 468.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.472435020944914 20.91560128700307 12.844883669214308 22.767080022837703 2 15.57633353466739 19.782346497503994 41.414619772927274 23.226700194901344 3 19.15235988228476 26.879405442065522 28.46700542164623 25.501229254003484 4 12.263883056840207 16.38461258670946 39.91320771160493 31.438296644845405 5 12.885699360631794 38.67886832890036 34.53496753640214 13.900464774065712 6 29.427372589727995 38.41474875675054 18.02131480345189 14.13656385006957 7 27.196771766957355 32.233459836048446 22.971113932423485 17.59865446457071 8 26.43594280554084 35.11178248115059 19.704362425264527 18.747912288044045 9 26.474545009028272 15.365866331861294 21.856136410975637 36.303452248134796 10-11 25.931212139645478 26.264368175782042 26.850646615468854 20.953773069103626 12-13 25.91513024737855 24.08538980828677 27.74075076496586 22.25872917936882 14-15 23.322078713552976 25.12217864157581 25.729773252341126 25.825969392530084 16-17 21.52098343680966 27.99168317970903 26.981660743219027 23.505672640262272 18-19 20.870248252644803 27.963494789625926 29.602572127019616 21.563684830709658 20-21 22.541960143788877 27.00283941211884 29.087481921386104 21.36771852270618 22-23 22.944264780178365 26.77468072138747 28.66740577275288 21.613648725681283 24-25 22.24503163051514 26.7367993319458 29.615588363507168 21.402580674031896 26-27 22.149718021267205 27.06316975463646 28.549948293244913 22.237163930851423 28-29 21.300398114680274 27.36336728211924 29.203953857645587 22.1322807455549 30-31 22.242975037485838 27.05944782005933 29.120949489090158 21.57662765336467 32-33 22.571258425628876 27.259736039654825 28.17877031895276 21.990235215763537 34-35 22.52201246989132 27.13606854118406 29.02141299744148 21.32050599148313 36-37 22.07838132918487 26.7295631743944 28.634709068949686 22.557346427471035 38-39 22.308581689581036 26.99718948328062 29.090313380424824 21.603915446713515 40-41 22.05587031006662 25.968257852802033 29.73043300883658 22.245438828294763 42-43 22.019340447387133 26.17593157253636 29.01457455810677 22.790153421969734 44-45 22.129796699922412 27.14864649648906 28.806704563327116 21.91485224026141 46-47 22.176164482552664 27.007971854000463 28.101780505008367 22.714083158438505 48-49 22.88360563810504 26.932980256114263 28.018318087021722 22.165096018758984 50-51 22.52611700613237 26.676213773802264 28.06926033195444 22.72840888811093 52-53 22.014976060860594 27.35269161386068 27.765515977549192 22.866816347729536 54-55 21.98165564065457 27.20100840127282 27.809417886533783 23.007918071538825 56-57 22.87113185359857 26.81581350419801 27.35501031733836 22.958044324865067 58-59 21.901909523663722 27.23496398705371 28.18886579481536 22.674260694467208 60-61 21.980134735248352 27.899391006473493 27.46674881659623 22.653725441681924 62-63 22.37432097040225 27.111103767864403 27.180303863648753 23.334271398084592 64-65 21.718102598144252 27.235618907029508 27.571347504128852 23.47493099069739 66-67 22.22091156653395 26.899854824872516 27.461744924442076 23.417488684151465 68-69 21.81623319353195 26.935410464770104 27.751102631095986 23.497253710601953 70-71 21.872157151196017 26.583314257185613 27.8664380890905 23.67809050252787 72-73 22.555692099650333 26.643836405965704 27.98329885409826 22.817172640285698 74-75 22.73558263068115 26.88260137302491 27.53126087518295 22.85055512111099 76-77 22.430890488317957 26.863067484585244 27.261044023394476 23.444998003702324 78-79 22.339944087791018 26.863857719134625 27.255633030582448 23.5405651624919 80-81 21.931406589226246 27.350941068821882 27.76731858131188 22.950333760639992 82-83 22.905699897423652 26.368954867397587 28.77887778973136 21.9464674454474 84-85 22.984379733490822 26.738492314105056 27.58354688668251 22.693581065721617 86-87 21.776376269547892 27.422981743970844 28.01913391244881 22.781508074032452 88-89 22.89990993047973 26.230028767506113 27.85000615829187 23.02005514372229 90-91 22.642701300946342 26.374729358728466 27.845230669983877 23.137338670341318 92-93 23.005973431886346 26.528854090737536 27.767261242793634 22.697911234582484 94-95 21.879671427714136 26.485194659356537 28.468516999793223 23.1666169131361 96-97 22.309961363304243 26.428747840973948 27.92651293443773 23.334777861284078 98-99 21.75412155888461 26.88375338285855 27.805737659454465 23.556387398802375 100 22.710237043902964 26.335395102735653 26.868061135080996 24.086306718280383 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 931.0 1 668.0 2 679.0 3 989.5 4 878.5 5 812.5 6 1195.0 7 2083.0 8 3369.5 9 4352.0 10 4488.0 11 4533.5 12 5236.0 13 6373.5 14 7697.5 15 9215.0 16 10896.5 17 12260.0 18 13400.5 19 14634.5 20 15775.5 21 16998.0 22 18717.0 23 21074.0 24 23806.0 25 27199.5 26 32878.5 27 39950.5 28 49316.5 29 68577.5 30 97888.5 31 126573.5 32 166565.5 33 212300.0 34 247580.0 35 282046.0 36 316577.0 37 366496.5 38 387517.0 39 368733.0 40 340390.5 41 309369.0 42 299114.5 43 310179.5 44 336922.5 45 393634.5 46 444981.5 47 477825.0 48 504368.0 49 500285.5 50 477673.0 51 484665.5 52 485961.0 53 456056.5 54 426514.0 55 365172.0 56 313998.0 57 257544.0 58 200330.0 59 190385.5 60 157670.0 61 107730.5 62 79684.0 63 67265.5 64 49921.5 65 24718.0 66 13777.0 67 9464.0 68 6947.0 69 5450.0 70 4400.0 71 3389.0 72 2626.0 73 2286.0 74 1929.0 75 1472.5 76 1082.5 77 763.5 78 532.0 79 391.0 80 307.5 81 239.0 82 180.0 83 137.0 84 93.0 85 77.0 86 65.5 87 44.0 88 30.5 89 29.5 90 32.5 91 24.5 92 17.0 93 12.0 94 9.0 95 11.0 96 8.5 97 4.5 98 4.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056728804000721944 2 0.13200113961704005 3 0.016671175025283567 4 0.01608606359081087 5 0.01884058819002079 6 0.050697655368464926 7 0.05583763427744814 8 0.047997141055514024 9 0.039913601545414325 10-11 0.03860385210363314 12-13 0.039738068115072515 14-15 0.018007929610194262 16-17 0.016792698169366356 18-19 0.01731029674601528 20-21 0.020654433636886144 22-23 0.025078776252937373 24-25 0.024399146817511395 26-27 0.019164649907574896 28-29 0.018178962183347816 30-31 0.01620758673489366 32-33 0.022990378517588672 34-35 0.0230713939469772 36-37 0.02294536994570616 38-39 0.020870474781922218 40-41 0.024489163961276424 42-43 0.03161852174746681 44-45 0.04049421212269877 46-47 0.04661987875590906 48-49 0.03908544382277605 50-51 0.027122165416403554 52-53 0.03845982467360909 54-55 0.0350346723533497 56-57 0.0373076052334167 58-59 0.041902980422621486 60-61 0.02226123965309193 62-63 0.025173294253890654 64-65 0.02432713310249937 66-67 0.030988401741111598 68-69 0.0377441883806771 70-71 0.039400503825953656 72-73 0.04255110385772971 74-75 0.02574490311679859 76-77 0.019664245055470816 78-79 0.01907013190662162 80-81 0.023471970236731584 82-83 0.01931767905197545 84-85 0.011792245833218936 86-87 0.014366736144898794 88-89 0.018174461326159567 90-91 0.02045639592060308 92-93 0.019326680766351953 94-95 0.023228923948566002 96-97 0.024430652817829152 98-99 0.027032148272638525 100 0.028463420858502505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1108995E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.09580344908346 #Duplication Level Percentage of deduplicated Percentage of total 1 75.39086466239891 46.81456313932775 2 14.777498909383324 18.35241335492224 3 5.004230942961252 9.322252231438306 4 2.0864343456222296 5.182352681407002 5 1.0194905941211445 3.1653043775367955 6 0.5258524582360532 1.9591938533906 7 0.3326290372768621 1.445840712414132 8 0.2122820991261279 1.0545462002475912 9 0.14186017416510188 0.7928029342988023 >10 0.4581325770068755 4.64092496443469 >50 0.024979770034120784 1.081621934524188 >100 0.022016825440128573 2.8015011019274763 >500 0.0025193938772486474 1.0807257169763 >1k 0.0011262977920208504 1.1175641937179803 >5k 2.0478139656173534E-5 0.09664209666704013 >10k+ 6.14344189685206E-5 1.0917505067691313 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37574 0.3382304159827239 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30016 0.2701954587251142 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18266 0.1644253148012039 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.800342875300601E-5 0.0 4 0.0 0.0 0.0 4.5008571882515024E-5 0.0 5 0.0 0.0 0.0 6.301200063552104E-5 0.0 6 0.0 0.0 0.0 6.301200063552104E-5 0.0 7 0.0 0.0 0.0 6.301200063552104E-5 0.0 8 0.0 0.0 0.0 6.301200063552104E-5 0.0 9 0.0 0.0 0.0 9.901885814153306E-5 0.0 10-11 0.0 0.0 0.0 9.901885814153306E-5 0.0 12-13 0.0 0.0 0.0 9.901885814153306E-5 0.0 14-15 0.0 0.0 0.0 1.1252142970628756E-4 0.0 16-17 0.0 0.0 0.0 1.2602400127104208E-4 0.0 18-19 0.0 0.0 0.0 1.530291444005511E-4 0.0 20-21 0.0 0.0 0.0 2.1154028784782064E-4 0.0 22-23 0.0 0.0 0.0 4.005762897543837E-4 0.0 24-25 0.0 0.0 0.0 7.561440076262524E-4 0.0 26-27 0.0 0.0 0.0 0.0015978043018292833 0.0 28-29 0.0 0.0 0.0 0.004968946335829659 0.0 30-31 0.0 0.0 0.0 0.01886309247596205 0.0 32-33 0.0 0.0 0.0 0.05045910993748759 0.0 34-35 0.0 0.0 0.0 0.09837973642080133 0.0 36-37 0.0 0.0 0.0 0.1782879549410185 0.0 38-39 0.0 0.0 0.0 0.29430204982538927 0.0 40-41 0.0 0.0 0.0 0.4163067856273227 0.0 42-43 0.0 0.0 0.0 0.5351834256834214 0.0 44-45 0.0 0.0 0.0 0.6735532782218374 0.0 46-47 0.0 0.0 0.0 0.8397294264692711 0.0 48-49 0.0 0.0 0.0 1.022536242027294 0.0 50-51 0.0 0.0 0.0 1.2124589127999428 0.0 52-53 0.0 0.0 0.0 1.3925922191881446 0.0 54-55 0.0 0.0 0.0 1.5851748965590495 0.0 56-57 0.0 0.0 0.0 1.8112259479817931 0.0 58-59 0.0 0.0 0.0 2.0727617574767114 4.500857188251502E-6 60-61 0.0 0.0 0.0 2.335427282125881 9.001714376503005E-6 62-63 0.0 0.0 0.0 2.598205328204757 9.001714376503005E-6 64-65 0.0 0.0 0.0 2.865457226328754 9.001714376503005E-6 66-67 0.0 0.0 0.0 3.161195049597196 9.001714376503005E-6 68-69 0.0 0.0 0.0 3.487673727461395 9.001714376503005E-6 70-71 0.0 0.0 0.0 3.8391051575772606 9.001714376503005E-6 72-73 0.0 0.0 0.0 4.1930525668613585 9.001714376503005E-6 74-75 0.0 0.0 0.0 4.530418818263938 9.001714376503005E-6 76-77 0.0 0.0 0.0 4.909399995229092 9.001714376503005E-6 78-79 0.0 0.0 0.0 5.3193290662206625 9.001714376503005E-6 80-81 0.0 0.0 0.0 5.753621277172238 9.001714376503005E-6 82-83 0.0 0.0 0.0 6.195137363910957 9.001714376503005E-6 84-85 0.0 0.0 0.0 6.6139646295637 9.001714376503005E-6 86-87 0.0 0.0 0.0 7.054616551722276 9.001714376503005E-6 88 0.0 0.0 0.0 7.412245662186363 9.001714376503005E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22365 0.0 47.03034 1 GTATCAA 39455 0.0 40.078064 1 GTACTAG 11965 0.0 37.051662 1 GGGCACC 12045 0.0 35.114685 94 GCATCGT 11765 0.0 34.642197 8 CAACGCA 43865 0.0 34.09159 5 ATCAACG 44755 0.0 33.58171 3 TAGGCAT 14500 0.0 32.87545 5 TACCTGG 5035 0.0 32.246983 2 TATCAAC 49775 0.0 31.61728 2 CTGTGCG 14720 0.0 31.551182 9 AACGCAG 50435 0.0 30.59189 6 GTGGTAT 7150 0.0 30.21177 1 CTAGGCA 16280 0.0 29.916246 4 ACTGTGC 16375 0.0 29.511417 8 TAGTACT 15875 0.0 29.465313 4 TCTAACG 5770 0.0 28.547083 2 TCAACGC 53715 0.0 28.295141 4 ACGCAGA 53495 0.0 27.928797 7 CGCAGAG 53605 0.0 27.816732 8 >>END_MODULE