FastQCFastQC Report
Mon 6 Jun 2016
ERR1211261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11108995
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343180.30892083397283016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291000.26194988835623745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179710.1617698090601355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA220550.046.9274751
GTATCAA388150.039.421411
GGGCACC123200.036.5560794
CAACGCA440850.033.1895755
ATCAACG448300.032.681823
GTGGTAT68900.032.2134321
CTGTGCG157100.031.0151969
TAGGCAT141450.030.7596915
TATCAAC499950.030.4497282
TACCTGG47700.030.2495612
AACGCAG505950.029.91286
TAGTACT168150.029.2956144
ACTGTGC176850.028.8268138
CTAGGCA161750.028.071854
TCAACGC535300.027.7214244
TGAACCG14100.027.6587735
GTACTGT184750.026.983916
TACTGTG201600.026.779567
ACGCAGA546750.026.6923377
CTAACGC54800.026.6758353
Produced by FastQC (version 0.11.5)