##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211261_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11108995 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.145982332335194 33.0 31.0 34.0 30.0 34.0 2 32.291705325279196 34.0 31.0 34.0 30.0 34.0 3 32.369960739022744 34.0 31.0 34.0 30.0 34.0 4 35.98351074962227 37.0 35.0 37.0 35.0 37.0 5 35.874350109978444 37.0 35.0 37.0 35.0 37.0 6 35.900692816946986 37.0 35.0 37.0 35.0 37.0 7 35.84001955172363 37.0 35.0 37.0 35.0 37.0 8 35.832618072111835 37.0 35.0 37.0 35.0 37.0 9 37.428380245017664 39.0 37.0 39.0 34.0 39.0 10-11 37.41056166646938 39.0 37.0 39.0 34.0 39.0 12-13 37.30326973772155 39.0 37.0 39.0 34.0 39.0 14-15 38.50373728676627 40.0 38.0 41.0 34.0 41.0 16-17 38.4666332102949 40.0 38.0 41.0 34.0 41.0 18-19 38.50114191247723 40.0 38.0 41.0 34.0 41.0 20-21 38.46743242750582 40.0 38.0 41.0 34.0 41.0 22-23 38.329409636065186 40.0 38.0 41.0 34.0 41.0 24-25 38.257383183627326 40.0 38.0 41.0 34.0 41.0 26-27 38.10923256334169 40.0 37.0 41.0 33.0 41.0 28-29 38.095465791459986 40.0 37.0 41.0 33.0 41.0 30-31 38.10373710673198 40.0 37.0 41.0 33.5 41.0 32-33 37.97376985946974 40.0 37.0 41.0 33.0 41.0 34-35 37.83160488415019 40.0 37.0 41.0 33.0 41.0 36-37 37.69381676740335 40.0 37.0 41.0 33.0 41.0 38-39 37.59487320860258 40.0 36.0 41.0 33.0 41.0 40-41 37.48378350156787 40.0 36.0 41.0 32.0 41.0 42-43 37.325552491471996 40.0 36.0 41.0 32.0 41.0 44-45 37.14294542395599 39.0 36.0 41.0 31.0 41.0 46-47 36.86902159016185 39.0 35.0 41.0 31.0 41.0 48-49 36.586230662629696 39.0 35.0 41.0 30.0 41.0 50-51 36.35855777232774 39.0 35.0 40.0 30.0 41.0 52-53 36.13029603487984 38.5 35.0 40.0 29.5 41.0 54-55 35.874825040428945 38.0 35.0 40.0 29.0 41.0 56-57 35.588534291355785 38.0 34.5 40.0 28.5 41.0 58-59 35.28298392428838 37.0 34.0 40.0 28.0 41.0 60-61 35.01630494027587 37.0 34.0 40.0 28.0 41.0 62-63 34.703082187002515 36.0 34.0 39.5 27.0 41.0 64-65 34.32852355231054 36.0 33.0 39.0 26.0 41.0 66-67 34.03408400129805 35.0 33.0 39.0 26.0 40.0 68-69 33.725449331825246 35.0 33.0 38.0 26.0 40.0 70-71 33.45425657316436 35.0 33.0 37.5 26.0 40.0 72-73 33.15278470284666 35.0 32.0 37.0 25.5 39.5 74-75 32.841988586726345 35.0 32.0 36.5 25.0 39.0 76-77 31.87433921790405 34.0 30.5 35.5 24.0 38.0 78-79 32.3953986836793 35.0 32.0 36.0 25.0 38.0 80-81 32.34716628281856 35.0 32.0 35.0 25.0 37.5 82-83 32.118991816991546 35.0 32.0 35.0 25.0 37.0 84-85 31.88901372266348 34.0 31.5 35.0 24.5 36.5 86-87 31.674973433690447 34.0 31.0 35.0 24.0 36.0 88-89 31.47253617451444 34.0 31.0 35.0 24.0 36.0 90-91 31.26405340897174 34.0 31.0 35.0 23.0 35.5 92-93 31.079429282306815 34.0 31.0 35.0 23.0 35.0 94-95 30.90511004820868 34.0 30.5 35.0 20.5 35.0 96-97 30.749306665454434 34.0 30.0 35.0 20.0 35.0 98-99 30.587750377059308 34.0 30.0 35.0 20.0 35.0 100 28.720294770138974 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3672237549660622 1101 2 -0.3293888969565586 1101 3 -0.3916010858696666 1101 4 -0.2243565117456754 1101 5 -0.2691136133599912 1101 6 -0.29636744319112296 1101 7 -0.27489061391135294 1101 8 -0.3218605266189556 1101 9 -0.4999954076629294 1101 10-11 -0.42492902718510805 1101 12-13 -0.46500008267116044 1101 14-15 -0.6967792616547044 1101 16-17 -0.6337925502628465 1101 18-19 -0.7318999162079294 1101 20-21 -0.7186063621136114 1101 22-23 -0.7433430379087795 1101 24-25 -0.7291690795696297 1101 26-27 -0.840099718253775 1101 28-29 -0.8983332404139546 1101 30-31 -0.6572809019430252 1101 32-33 -0.6980408015777755 1101 34-35 -0.6828166647985796 1101 36-37 -0.5865720289713181 1101 38-39 -0.7034522972254891 1101 40-41 -0.7206840301842519 1101 42-43 -0.6887205434549202 1101 44-45 -0.7703623199036755 1101 46-47 -0.680641474134255 1101 48-49 -0.669647715763773 1101 50-51 -0.8380891361451361 1101 52-53 -0.7857413800163044 1101 54-55 -0.7516016617330905 1101 56-57 -0.7123261309783473 1101 58-59 -0.6749544625221517 1101 60-61 -0.7634480621445121 1101 62-63 -0.8210931396087773 1101 64-65 -0.780253881174076 1101 66-67 -0.8902293243433448 1101 68-69 -0.9059221292281805 1101 70-71 -0.9451391890079535 1101 72-73 -1.0401659201441404 1101 74-75 -1.0056444009354166 1101 76-77 -0.9778814933043201 1101 78-79 -0.834658086347531 1101 80-81 -0.7936787495431261 1101 82-83 -0.7789014129990086 1101 84-85 -0.7837926802925956 1101 86-87 -0.9215152109608518 1101 88-89 -0.8262707986302047 1101 90-91 -0.8917621113207019 1101 92-93 -0.9683009149490012 1101 94-95 -0.9763940828417432 1101 96-97 -1.0485948206373692 1101 98-99 -1.1626717860635054 1101 100 -1.0983803113433623 1102 1 -0.38972145104779443 1102 2 -0.3454441469162681 1102 3 -0.3786163781198013 1102 4 -0.29572466715983836 1102 5 -0.35298904549444643 1102 6 -0.39792726009081036 1102 7 -0.3998390512423029 1102 8 -0.36003722231969704 1102 9 -0.5028197749585317 1102 10-11 -0.515572172579418 1102 12-13 -0.5221773420058895 1102 14-15 -0.6559868555924595 1102 16-17 -0.7173174004443155 1102 18-19 -0.6371792047664613 1102 20-21 -0.7311348286355113 1102 22-23 -0.6544518004903637 1102 24-25 -0.7733142917135751 1102 26-27 -0.7880992379450618 1102 28-29 -0.8424386000165285 1102 30-31 -0.7572234041652237 1102 32-33 -0.6467007948630368 1102 34-35 -0.6519566627290061 1102 36-37 -0.7457594879323466 1102 38-39 -0.8008423000077158 1102 40-41 -0.6721515862457395 1102 42-43 -0.6270357345204332 1102 44-45 -0.6788195532563108 1102 46-47 -0.7500830272497794 1102 48-49 -0.7450064878328959 1102 50-51 -0.7653429463022761 1102 52-53 -0.8785354007659905 1102 54-55 -0.7661279754300665 1102 56-57 -0.8033365498982263 1102 58-59 -0.8419988936713949 1102 60-61 -0.8894750382793859 1102 62-63 -0.9143320234241159 1102 64-65 -0.828890948223453 1102 66-67 -0.9249692895800834 1102 68-69 -1.0065475667251533 1102 70-71 -0.9149426413456965 1102 72-73 -0.9671612147215711 1102 74-75 -1.0905222801064625 1102 76-77 -0.9041909749189792 1102 78-79 -0.7120283142077781 1102 80-81 -0.8052204980978601 1102 82-83 -0.7690091149414044 1102 84-85 -0.9345668844380199 1102 86-87 -0.8663822641321168 1102 88-89 -0.8703665007903965 1102 90-91 -0.8691266496087238 1102 92-93 -0.8959859363413223 1102 94-95 -1.0859303843750467 1102 96-97 -1.1694531400942871 1102 98-99 -1.1173608696512858 1102 100 -1.1920989882495974 1103 1 -0.3746239225679737 1103 2 -0.3553793030553507 1103 3 -0.4269590594930115 1103 4 -0.28194815275006135 1103 5 -0.2729311883147645 1103 6 -0.35384336813046957 1103 7 -0.3911154292599619 1103 8 -0.3824867016106239 1103 9 -0.5187746733913414 1103 10-11 -0.5130240550638092 1103 12-13 -0.5556821298640813 1103 14-15 -0.6861940571912513 1103 16-17 -0.7426024253262753 1103 18-19 -0.6645220135445484 1103 20-21 -0.6861207533253619 1103 22-23 -0.6900369937384028 1103 24-25 -0.7923669094959891 1103 26-27 -0.704840887283261 1103 28-29 -0.833990207032393 1103 30-31 -0.7967227641318573 1103 32-33 -0.7502275881739138 1103 34-35 -0.8355483010885365 1103 36-37 -0.8057472203621217 1103 38-39 -0.8013559430201767 1103 40-41 -0.8348925123296098 1103 42-43 -0.7049376411119823 1103 44-45 -0.823973255294689 1103 46-47 -0.8659060598439652 1103 48-49 -0.9132602387744697 1103 50-51 -0.8790969560127593 1103 52-53 -0.8766769591945902 1103 54-55 -0.8867263009228736 1103 56-57 -0.8432344806892544 1103 58-59 -0.8280630093290569 1103 60-61 -0.8930983324772228 1103 62-63 -0.7993132490195265 1103 64-65 -0.879086845113072 1103 66-67 -0.9313593146960315 1103 68-69 -0.9297562265120618 1103 70-71 -0.9517217803040481 1103 72-73 -0.8985356101719262 1103 74-75 -0.8728356214567761 1103 76-77 -0.8651176819524302 1103 78-79 -0.6627251027336385 1103 80-81 -0.6831292431011349 1103 82-83 -0.773486987903766 1103 84-85 -0.850346167627567 1103 86-87 -0.7737453915744545 1103 88-89 -0.8867839892212217 1103 90-91 -0.8018306801402879 1103 92-93 -1.0286831779503167 1103 94-95 -1.0507311273696196 1103 96-97 -1.0376455997046037 1103 98-99 -1.0735952721536464 1103 100 -1.1227854448251584 1104 1 -0.41723196336683444 1104 2 -0.3958386373395726 1104 3 -0.43610324636284403 1104 4 -0.2810964506667517 1104 5 -0.28743065968273385 1104 6 -0.37650932224138955 1104 7 -0.41926817509024517 1104 8 -0.4168468775217775 1104 9 -0.6480840078780261 1104 10-11 -0.5735417548366755 1104 12-13 -0.6457297568818348 1104 14-15 -0.7988897151583316 1104 16-17 -0.7568384773175225 1104 18-19 -0.8441816306271477 1104 20-21 -0.8274590697282704 1104 22-23 -0.7980889668577049 1104 24-25 -0.8850994386555939 1104 26-27 -0.9117879442499941 1104 28-29 -0.9899108424243792 1104 30-31 -0.8906126918163793 1104 32-33 -0.818246516729431 1104 34-35 -0.9175476186778937 1104 36-37 -0.8823424926196708 1104 38-39 -0.8368996887430598 1104 40-41 -0.9395980798092936 1104 42-43 -0.8709539335454792 1104 44-45 -0.9421991390010405 1104 46-47 -1.0359124250248684 1104 48-49 -1.0290244981969607 1104 50-51 -0.9852978589390275 1104 52-53 -0.94528438256512 1104 54-55 -0.991404091840657 1104 56-57 -1.004366350037408 1104 58-59 -0.9585541830155861 1104 60-61 -1.0159563073778557 1104 62-63 -1.0474024847678223 1104 64-65 -1.028119539119487 1104 66-67 -1.0472510850242642 1104 68-69 -1.0801553277893987 1104 70-71 -1.0613521485994681 1104 72-73 -0.9966954924864595 1104 74-75 -1.0964931390516526 1104 76-77 -1.0254629616819813 1104 78-79 -0.9368872985725751 1104 80-81 -0.8875208178075553 1104 82-83 -0.8961311093092306 1104 84-85 -0.8578867569843105 1104 86-87 -0.9108117720618729 1104 88-89 -0.9406600199476323 1104 90-91 -0.9586824666813136 1104 92-93 -1.0483151428436734 1104 94-95 -1.0668662342635855 1104 96-97 -1.1637005932478317 1104 98-99 -1.1919867739718981 1104 100 -1.1982865461499266 1105 1 -0.402904599861003 1105 2 -0.36421977527551874 1105 3 -0.40575342999659725 1105 4 -0.2625345714319067 1105 5 -0.28321716987010603 1105 6 -0.3519757490074156 1105 7 -0.39638203988359066 1105 8 -0.4396359671848842 1105 9 -0.5986513367930755 1105 10-11 -0.5468658001784945 1105 12-13 -0.5784134314258722 1105 14-15 -0.8197044082318641 1105 16-17 -0.7396214965352854 1105 18-19 -0.6793413200517406 1105 20-21 -0.8246721345726371 1105 22-23 -0.8168757529407031 1105 24-25 -0.8087574141157106 1105 26-27 -0.8237826547672569 1105 28-29 -0.8119192290579775 1105 30-31 -0.8007012379947795 1105 32-33 -0.783049985597728 1105 34-35 -0.8462690019236163 1105 36-37 -0.7402013089681247 1105 38-39 -0.8492689349843516 1105 40-41 -0.8836466515302916 1105 42-43 -0.8731516980492842 1105 44-45 -0.9033368858237907 1105 46-47 -0.7977482730627443 1105 48-49 -0.9217089996862029 1105 50-51 -0.9135222592368777 1105 52-53 -0.9594604855542812 1105 54-55 -0.8529016710790671 1105 56-57 -0.9151786030663303 1105 58-59 -1.0033170425698756 1105 60-61 -0.945076014928496 1105 62-63 -0.9961445453521307 1105 64-65 -0.9705834539003746 1105 66-67 -1.01545213060934 1105 68-69 -0.9811912898938004 1105 70-71 -0.9988709495511543 1105 72-73 -0.9676499504257308 1105 74-75 -1.043463404623438 1105 76-77 -1.003990402171592 1105 78-79 -0.9661649415339717 1105 80-81 -0.9375117510764142 1105 82-83 -0.8625011943622098 1105 84-85 -0.946117843301078 1105 86-87 -0.9297100845003143 1105 88-89 -0.9776006267400028 1105 90-91 -0.9428094387416905 1105 92-93 -0.9649436204634938 1105 94-95 -0.9788329658497794 1105 96-97 -1.0318128998242315 1105 98-99 -1.0586299363587237 1105 100 -1.1967733847420874 1106 1 -0.47217007611702044 1106 2 -0.4174236316530866 1106 3 -0.46187299756862643 1106 4 -0.3256096126029817 1106 5 -0.34529004985881073 1106 6 -0.4166862259695705 1106 7 -0.44407122337973703 1106 8 -0.44638008744033897 1106 9 -0.5630867888610851 1106 10-11 -0.6156236319587336 1106 12-13 -0.6234090472745777 1106 14-15 -0.7359176744148499 1106 16-17 -0.6782360665787763 1106 18-19 -0.7572209130505101 1106 20-21 -0.7987694938489369 1106 22-23 -0.7444784622130527 1106 24-25 -0.7669371288623736 1106 26-27 -0.8219096848963403 1106 28-29 -0.8157424393727695 1106 30-31 -0.868960663359033 1106 32-33 -0.8522409215575522 1106 34-35 -0.8891087463457694 1106 36-37 -0.8446678510344299 1106 38-39 -0.8880295149938817 1106 40-41 -0.8302093564880622 1106 42-43 -0.7459140866240688 1106 44-45 -0.772858024449441 1106 46-47 -0.8119608971444734 1106 48-49 -0.8596846288616575 1106 50-51 -0.8764337867496863 1106 52-53 -0.8417967392614756 1106 54-55 -0.8236398361894501 1106 56-57 -0.8102003959111812 1106 58-59 -0.846820924948652 1106 60-61 -0.8789175562467832 1106 62-63 -0.8748991434374318 1106 64-65 -0.8735230307059751 1106 66-67 -0.9326222840015248 1106 68-69 -0.9499968117551632 1106 70-71 -1.0196071024849616 1106 72-73 -1.0628546643346084 1106 74-75 -1.0122488608205202 1106 76-77 -0.9928862356046615 1106 78-79 -0.8236969299055374 1106 80-81 -0.7974445690631455 1106 82-83 -0.7840944335386482 1106 84-85 -0.7931554331533626 1106 86-87 -0.9056982913502694 1106 88-89 -0.9400195637003819 1106 90-91 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1.1773804203227556 2310 68-69 1.300185524553008 2310 70-71 1.3414927657002593 2310 72-73 1.3405237568520931 2310 74-75 1.3048214573422001 2310 76-77 1.2702947365803006 2310 78-79 1.1194792192251484 2310 80-81 1.1097137698400843 2310 82-83 1.1586353401123404 2310 84-85 1.195216050298395 2310 86-87 1.175604335432606 2310 88-89 1.19279404742975 2310 90-91 1.262366548272805 2310 92-93 1.2591900664370819 2310 94-95 1.363598955202292 2310 96-97 1.3787418962000402 2310 98-99 1.3627153295985153 2310 100 1.4644138764295214 2311 1 0.3526138347076184 2311 2 0.2965268620685748 2311 3 0.37709471659587734 2311 4 0.2579095656155772 2311 5 0.37615234051877167 2311 6 0.4416616362250281 2311 7 0.47539810585541886 2311 8 0.49707497596618566 2311 9 0.6048327005181022 2311 10-11 0.661693436433076 2311 12-13 0.7184768473937595 2311 14-15 0.8228936823881057 2311 16-17 0.6690741610985995 2311 18-19 0.8082342488107059 2311 20-21 0.7231085615626753 2311 22-23 0.8972821096176204 2311 24-25 0.9446962569152149 2311 26-27 0.35329485806336436 2311 28-29 0.6868927903917452 2311 30-31 0.867755970785538 2311 32-33 0.9769106686012634 2311 34-35 1.0192209644067702 2311 36-37 1.0283370420053188 2311 38-39 1.061730116831832 2311 40-41 1.0349810760529508 2311 42-43 1.0396409556311283 2311 44-45 1.056716774825908 2311 46-47 0.6856851808905375 2311 48-49 0.9529555838783565 2311 50-51 1.082824607881527 2311 52-53 1.0103926392423972 2311 54-55 1.0293106493783597 2311 56-57 1.1476125616019104 2311 58-59 1.1097095355199045 2311 60-61 1.1585934970869332 2311 62-63 1.164584122186504 2311 64-65 1.2563739034223644 2311 66-67 1.2049027434477395 2311 68-69 1.2422563042931003 2311 70-71 1.271477268800929 2311 72-73 1.2894541924007967 2311 74-75 1.2877525813155017 2311 76-77 1.2106604788930504 2311 78-79 0.9826028739883057 2311 80-81 0.9102687776197769 2311 82-83 1.0747310525463831 2311 84-85 1.0330972810439967 2311 86-87 1.1054098222408761 2311 88-89 1.0993946376033108 2311 90-91 1.120845024084911 2311 92-93 1.194413840288771 2311 94-95 1.2610581664309564 2311 96-97 1.2644041541370896 2311 98-99 1.3895260386823232 2311 100 1.4342152547528748 2312 1 0.20695242580934803 2312 2 0.3463217167532022 2312 3 0.44327581536797567 2312 4 0.1754574883997222 2312 5 0.37002857849545734 2312 6 0.451468272009933 2312 7 0.3562001238663228 2312 8 0.4387119693165644 2312 9 0.6202719454535099 2312 10-11 0.6138955052932005 2312 12-13 0.7142756163286634 2312 14-15 0.8910771021219617 2312 16-17 0.8565101769538259 2312 18-19 0.8134287253525443 2312 20-21 0.8538317797002364 2312 22-23 0.9221382654357058 2312 24-25 0.9173703662375701 2312 26-27 0.9950712255200926 2312 28-29 0.9736721784700464 2312 30-31 0.9525876810137461 2312 32-33 0.8116517456928989 2312 34-35 0.96219060054311 2312 36-37 1.0206747867147925 2312 38-39 0.8343214826647483 2312 40-41 0.9675859086224179 2312 42-43 1.0571230081932157 2312 44-45 0.7949492586500853 2312 46-47 0.8390330791194529 2312 48-49 1.0454511794894827 2312 50-51 0.8075842694753135 2312 52-53 1.0409897751676667 2312 54-55 0.9120105175413187 2312 56-57 0.961093865142594 2312 58-59 1.076669398250182 2312 60-61 1.126336190554028 2312 62-63 1.1443936650902415 2312 64-65 1.1399712280036525 2312 66-67 1.1492375638439185 2312 68-69 1.1886972281995156 2312 70-71 1.0594289227240594 2312 72-73 1.0911480081152618 2312 74-75 1.1320430414642573 2312 76-77 1.1297359189890166 2312 78-79 1.0724900319900925 2312 80-81 0.9859963977312702 2312 82-83 0.9778750564048977 2312 84-85 1.0552483166622117 2312 86-87 1.1058635554374057 2312 88-89 1.036794030716294 2312 90-91 1.1437833098015169 2312 92-93 1.1641336184725724 2312 94-95 1.169326516634495 2312 96-97 0.841050699587349 2312 98-99 0.6174742696529378 2312 100 0.8461535587265168 2313 1 0.05128696125050425 2313 2 0.24263221406118873 2313 3 0.2847464411726719 2313 4 0.19834314057955993 2313 5 0.3416723390968741 2313 6 0.2884284104365733 2313 7 0.30680089432352275 2313 8 0.43130623264629975 2313 9 0.608606095766639 2313 10-11 0.36485093047334516 2313 12-13 0.40260278551059514 2313 14-15 0.5164548238373428 2313 16-17 0.7448182726063806 2313 18-19 0.6951295597874534 2313 20-21 0.6995537178158671 2313 22-23 0.6641324120749417 2313 24-25 0.551208503476083 2313 26-27 0.7274932789272555 2313 28-29 0.573541249114605 2313 30-31 0.8112378671818163 2313 32-33 0.9099774811598706 2313 34-35 0.9467753073579956 2313 36-37 0.9865250313469716 2313 38-39 1.0170195376310787 2313 40-41 0.9829417799000524 2313 42-43 1.0512196662749744 2313 44-45 0.8734487415343324 2313 46-47 0.9040459948979347 2313 48-49 0.9672311818723074 2313 50-51 0.9085015498826223 2313 52-53 0.9492617230075027 2313 54-55 0.9068963703315234 2313 56-57 0.6178097910732916 2313 58-59 0.6889668597243528 2313 60-61 0.5479482480419762 2313 62-63 0.9382498330316622 2313 64-65 1.0144074685140865 2313 66-67 1.0059841345175116 2313 68-69 1.0837371779170795 2313 70-71 0.9762779925877609 2313 72-73 1.0365485673189525 2313 74-75 1.105018600281639 2313 76-77 1.0886222109458679 2313 78-79 0.9595651030609318 2313 80-81 0.870446526898192 2313 82-83 0.9164739045136727 2313 84-85 0.9286846369961985 2313 86-87 0.899419962200259 2313 88-89 0.8947504469701926 2313 90-91 0.8193406550624793 2313 92-93 0.9989558826211997 2313 94-95 1.1477111491668595 2313 96-97 1.1708623105389222 2313 98-99 1.1757241060055321 2313 100 1.2678686073297278 2314 1 -0.3961620266794519 2314 2 0.03353978887565745 2314 3 0.28750663770836127 2314 4 0.24353881767588348 2314 5 0.3328808858889616 2314 6 0.3877614860698131 2314 7 0.4171082140025817 2314 8 0.44190106961304565 2314 9 0.4919817529750148 2314 10-11 0.5531411226862488 2314 12-13 0.45645044465739204 2314 14-15 0.7660331935129747 2314 16-17 0.7915085816509517 2314 18-19 0.7985570914571625 2314 20-21 0.8438797153847943 2314 22-23 0.8810868566768022 2314 24-25 0.7970208027211498 2314 26-27 0.7338211073633971 2314 28-29 0.7570388596588984 2314 30-31 0.8258734744985219 2314 32-33 0.9018322302925981 2314 34-35 0.9645978939969524 2314 36-37 0.9030540689851563 2314 38-39 0.6579870756676343 2314 40-41 0.6397241407333709 2314 42-43 0.8680062989778392 2314 44-45 0.9548539322380094 2314 46-47 0.8922666250221809 2314 48-49 0.945685611374202 2314 50-51 0.9513423675494437 2314 52-53 0.9163144768077274 2314 54-55 0.8007371648809993 2314 56-57 0.8201042820123448 2314 58-59 0.9587623444782238 2314 60-61 0.9873599173647207 2314 62-63 0.9126397094739715 2314 64-65 0.9311694227377458 2314 66-67 0.6412831090774631 2314 68-69 0.6650845395188156 2314 70-71 0.7937192299388514 2314 72-73 0.9632641656597016 2314 74-75 1.016000489987448 2314 76-77 0.8584342968559007 2314 78-79 0.8154340439771133 2314 80-81 0.8522305522462403 2314 82-83 0.836124754100247 2314 84-85 0.8261075447792727 2314 86-87 0.9237734926134529 2314 88-89 0.8747942488375315 2314 90-91 1.045430800033298 2314 92-93 0.949883436891362 2314 94-95 0.8942953361867403 2314 96-97 0.8645584171317395 2314 98-99 1.0392349511740733 2314 100 1.021365704937331 2315 1 0.42710757958765555 2315 2 0.4458247159252764 2315 3 0.4552295164241542 2315 4 0.3142128932999384 2315 5 0.3849826847953963 2315 6 0.43058701538048894 2315 7 0.4035717918687851 2315 8 0.26825383567079086 2315 9 0.49591425589459703 2315 10-11 0.5535959353416118 2315 12-13 0.382638378138914 2315 14-15 0.5954742389736865 2315 16-17 0.7261847293197192 2315 18-19 0.7400208228281784 2315 20-21 0.5070690854607207 2315 22-23 0.8411421566234409 2315 24-25 0.8861261651730175 2315 26-27 0.8392194299433129 2315 28-29 0.8031250221550934 2315 30-31 0.8533477348118907 2315 32-33 0.8056129269575223 2315 34-35 0.9022098106584195 2315 36-37 0.8635381907804742 2315 38-39 0.8966568791618172 2315 40-41 0.8736458463961227 2315 42-43 0.9693994134580208 2315 44-45 0.9566598852374355 2315 46-47 0.8996819573162966 2315 48-49 0.807320266601657 2315 50-51 0.9446395304223998 2315 52-53 0.9348931265792189 2315 54-55 0.803272275463712 2315 56-57 0.7728280018431164 2315 58-59 0.8032307278610205 2315 60-61 0.8881570248494839 2315 62-63 0.9872238430577696 2315 64-65 0.9918301856427618 2315 66-67 1.0037980423710593 2315 68-69 1.056286036714063 2315 70-71 0.975060556002795 2315 72-73 1.0022424929257028 2315 74-75 0.4879883054883649 2315 76-77 0.5082485237282306 2315 78-79 0.79216514130853 2315 80-81 0.807609598845076 2315 82-83 0.7394951185719165 2315 84-85 0.8330030971503142 2315 86-87 0.8211993994417845 2315 88-89 0.839518197919972 2315 90-91 0.8866801617042732 2315 92-93 0.9745276156172196 2315 94-95 1.0423615162733455 2315 96-97 1.1310040868279785 2315 98-99 1.0676981211536898 2315 100 1.0283014315024346 2316 1 0.2964949921468758 2316 2 0.14478222088641246 2316 3 0.2403613530861648 2316 4 0.017852782951521817 2316 5 0.25845317483572217 2316 6 0.3682658835285437 2316 7 0.41867258350171 2316 8 0.44270668911451594 2316 9 0.5984649669712212 2316 10-11 0.5923851988595104 2316 12-13 0.3618287508455893 2316 14-15 0.4913115592620798 2316 16-17 0.7925357429604603 2316 18-19 0.7483042630247994 2316 20-21 0.7383460819108976 2316 22-23 0.7581510385721231 2316 24-25 0.45819816815691894 2316 26-27 0.7061308096033514 2316 28-29 0.8825736710503804 2316 30-31 0.47979398051677435 2316 32-33 0.6590461606354054 2316 34-35 0.8338158529732382 2316 36-37 0.8922116405708351 2316 38-39 0.7816289415560291 2316 40-41 0.7637591444101375 2316 42-43 0.7184246019161264 2316 44-45 0.5296167411018828 2316 46-47 0.7194595739062066 2316 48-49 0.8497666440757428 2316 50-51 0.8594157358347019 2316 52-53 0.8746656669480259 2316 54-55 0.7978734081188747 2316 56-57 0.9209872848693692 2316 58-59 0.7775010575825334 2316 60-61 0.8720668855626812 2316 62-63 0.9074182881484418 2316 64-65 0.9514534337736578 2316 66-67 0.9538245444259914 2316 68-69 0.9733469286299155 2316 70-71 0.9210023996097476 2316 72-73 0.8745471242045397 2316 74-75 0.9125792327828535 2316 76-77 0.8232909030271465 2316 78-79 0.5501519004475597 2316 80-81 0.5433544488208071 2316 82-83 0.4679547277725007 2316 84-85 0.6196604295983228 2316 86-87 0.6905411423696997 2316 88-89 0.6383569843465366 2316 90-91 0.5006216457071382 2316 92-93 0.33427448986732244 2316 94-95 0.41245314527009214 2316 96-97 0.6549564347081436 2316 98-99 0.830636424591578 2316 100 0.47964122571303136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 6.0 12 42.0 13 216.0 14 913.0 15 2732.0 16 6632.0 17 13998.0 18 24582.0 19 36634.0 20 54372.0 21 78248.0 22 94757.0 23 92743.0 24 89323.0 25 94748.0 26 110340.0 27 135788.0 28 167926.0 29 207951.0 30 256572.0 31 322732.0 32 412099.0 33 548453.0 34 797254.0 35 1110112.0 36 1751359.0 37 2475404.0 38 1898757.0 39 324267.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.76799042025054 21.67171563122143 13.610032620822807 22.95026132770522 2 15.270751314587864 20.1528581118274 41.110676528344825 23.465714045239917 3 18.71725570134832 26.865004440095618 29.109131834157814 25.30860802439825 4 12.183448027822399 16.756645582187282 39.77684304443157 31.283063345558748 5 12.729621356387325 38.91073855015688 34.63254776872255 13.727092324733245 6 29.624786040501412 38.52062225250799 17.762227816287613 14.092363890702982 7 27.056254103686463 32.293813778736606 22.968527796686516 17.68140432089041 8 26.59205445677129 35.30526388750738 19.503429428134588 18.599252227586742 9 26.318274222860932 15.269512910837745 21.85413424001471 36.55807862628661 10-11 25.955759763512926 26.41724856302633 26.76417644640527 20.862815227055478 12-13 25.82543492694927 24.076464363283364 27.818234993778013 22.279865715989352 14-15 23.233980368492908 25.204156925672468 25.658510111505294 25.903352594329327 16-17 21.451279918793524 28.027877300737202 26.978544763817585 23.54229801665168 18-19 20.877307083133985 27.893792372757392 29.626073285657256 21.60282725845137 20-21 22.533087940587244 26.975078617601262 29.09269151226963 21.39914192954187 22-23 22.82111028045291 26.742747656291137 28.718052353070643 21.718089710185303 24-25 22.124142986922596 26.735436853875783 29.68964073234043 21.450779426861196 26-27 22.078921798701696 27.033559259357276 28.61352013053264 22.27399881140839 28-29 21.268761176770717 27.36158087629663 29.27109946567542 22.098558481257232 30-31 22.2591221414984 26.96218148858017 29.194975679705497 21.58372069021593 32-33 22.596657260412755 27.201464726907954 28.277839856422695 21.924038156256593 34-35 22.46021059781752 27.0882581803258 29.11780537142748 21.333725850429197 36-37 22.09581895683096 26.68634369293934 28.743021704729166 22.474815645500527 38-39 22.223791868822442 26.872456339664563 29.305294523025815 21.59845726848718 40-41 21.900838051940173 25.922259515784614 29.9337609680491 22.243141464226117 42-43 21.97914175252883 26.108762212072083 29.16070115123908 22.751394884160007 44-45 22.05369934315014 27.066172797026805 28.906619989512766 21.97350787031029 46-47 22.0729061768621 26.93056145147909 28.20811892304098 22.788413448617835 48-49 22.91808467093464 26.86714535160009 28.15971709920279 22.055052878262472 50-51 22.48154576094916 26.62048177911162 28.109933374421775 22.78803908551744 52-53 21.91062723148112 27.264417397015954 27.84701182852106 22.977943542981862 54-55 21.933643498394694 27.1779396713081 27.823204114495976 23.06521271580123 56-57 22.88142291138076 26.684849916400683 27.433886143252884 22.999841028965676 58-59 21.760225004358325 27.223491896846173 28.250737764404494 22.765545334391003 60-61 21.95550001849935 27.868058501873204 27.46414254527097 22.71229893435648 62-63 22.375769386154616 27.074327005409017 27.146751204624493 23.403152403811877 64-65 21.586149429657848 27.239283012328315 27.531771709694635 23.642795848319203 66-67 22.07107475012959 26.92117952614131 27.5080557958721 23.499689927857 68-69 21.678841791204484 26.88890068819211 27.78433269603585 23.647924824567554 70-71 21.649479568624987 26.49356360823414 27.939548560490664 23.917408262650213 72-73 22.215695016319188 26.596180647943214 28.03157506586732 23.15654926987028 74-75 22.353471458401405 26.958827538440627 27.561731767725593 23.12596923543238 76-77 22.040157837057993 26.88515203295375 27.264297381924194 23.810392748064057 78-79 21.945372246882343 26.84416461745857 27.34357764071225 23.866885494946832 80-81 21.465154157865893 27.421582497304858 27.781623552778356 23.331639792050893 82-83 22.457535006952853 26.34678770268048 28.794563861337725 22.40111342902894 84-85 22.54962149072227 26.78142217824302 27.540660808700824 23.128295522333886 86-87 21.183268991835387 27.510535476511755 28.01902868484081 23.287166846812053 88-89 22.187914088489613 26.34708981649715 27.857427897369565 23.607568197643676 90-91 21.92533700109307 26.364505778711926 27.945520186743057 23.764637033451947 92-93 22.220157514719332 26.593673277943143 27.749737879203895 23.436431328133633 94-95 21.03218674426431 26.5227024779713 28.490737024551038 23.954373753213353 96-97 21.383665434798598 26.513468885331488 27.985242608814087 24.11762307105583 98-99 20.738638307619336 27.010645627275913 27.919034492495047 24.331681572609703 100 21.40657821941373 26.448550683921013 27.216782214307738 24.928088882357518 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 784.0 1 557.5 2 459.5 3 660.5 4 601.0 5 561.0 6 915.5 7 1772.5 8 2858.5 9 3609.0 10 3742.5 11 3803.5 12 4511.5 13 5522.5 14 6842.5 15 8504.5 16 10138.0 17 11891.5 18 13637.5 19 14939.5 20 16204.0 21 17785.5 22 19666.5 23 22116.5 24 24815.5 25 28484.0 26 34484.0 27 41454.0 28 50972.0 29 70301.0 30 99225.0 31 127383.5 32 166997.0 33 212429.5 34 248230.5 35 283227.5 36 317308.5 37 367792.0 38 388797.0 39 369064.0 40 340382.5 41 309472.0 42 300922.5 43 311980.5 44 338645.0 45 399465.0 46 451953.0 47 483897.5 48 511725.5 49 505211.0 50 479494.5 51 481124.0 52 479975.5 53 448210.0 54 420225.5 55 363906.0 56 312316.5 57 256188.5 58 199696.5 59 192613.5 60 159156.0 61 105428.0 62 76444.0 63 64139.0 64 46248.0 65 21333.0 66 11417.0 67 7952.0 68 5962.5 69 4712.5 70 3862.5 71 2917.0 72 2242.0 73 2023.5 74 1659.0 75 1212.5 76 804.5 77 472.0 78 319.5 79 220.5 80 145.0 81 95.5 82 62.5 83 38.5 84 24.5 85 16.0 86 8.5 87 6.0 88 3.0 89 2.5 90 2.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042650122715871236 2 0.0 3 0.0 4 0.03588083350474098 5 0.0 6 0.0 7 7.291388644967433E-4 8 0.0 9 0.011108115540604709 10-11 5.220994338371742E-4 12-13 2.7005143129509014E-5 14-15 1.4402743002404808E-4 16-17 2.5654885973033564E-4 18-19 0.0 20-21 4.500857188251502E-6 22-23 0.0 24-25 5.671080057196893E-4 26-27 4.4108400444864724E-4 28-29 1.530291444005511E-4 30-31 0.0024169603100910567 32-33 0.015019360437195265 34-35 0.026077966548729205 36-37 0.03140698145961899 38-39 0.022153219080573893 40-41 0.0012332348695809116 42-43 7.201371501202404E-5 44-45 0.010851566680874372 46-47 0.01782339446547595 48-49 0.0032946274618001 50-51 0.011243141256252254 52-53 0.010640026393026553 54-55 0.010315964675472444 56-57 4.7709086195465923E-4 58-59 0.008493117514230585 60-61 0.004464850330745491 62-63 0.0012512382983339177 64-65 0.03556127264437512 66-67 0.06067155489763025 68-69 0.05981189117467421 70-71 0.05222794681247043 72-73 0.045400146457892906 74-75 0.035237210926821014 76-77 0.03142948574556024 78-79 0.014902338150300724 80-81 0.0011072108683098695 82-83 0.03781620209568912 84-85 0.05190838595210458 86-87 0.05750745229428945 88-89 0.07025838070860596 90-91 0.04857325077561021 92-93 0.03944551239783617 94-95 0.012971470416540832 96-97 0.003528672035589178 98-99 0.0221712225093269 100 0.032577204328564376 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1108995E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.89339027006877 #Duplication Level Percentage of deduplicated Percentage of total 1 74.19834779741072 45.181889498220286 2 15.363028406233406 18.710137689418467 3 5.290834664084388 9.665305801634963 4 2.218756071572285 5.404303175213432 5 1.0716350637804377 3.2627746082936113 6 0.5800838138818936 2.119396204083605 7 0.3550789835716432 1.5135374186329185 8 0.22693691879671446 1.1055166690380192 9 0.14653078955536786 0.8030480899478699 >10 0.4960136382507867 4.886572925830693 >50 0.02586679476451289 1.0927366191462997 >100 0.0229507914506248 2.8729635933268516 >500 0.002652438206260044 1.107415115336899 >1k 0.0011996429768961379 1.1355352745568452 >5k 2.1046365951729882E-5 0.09579844703098679 >10k+ 6.313909785518964E-5 1.0430688702882922 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34318 0.30892083397283016 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29100 0.26194988835623745 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17971 0.1617698090601355 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.800342875300601E-5 0.0 4 0.0 0.0 0.0 4.5008571882515024E-5 0.0 5 0.0 9.001714376503005E-6 0.0 5.401028625901803E-5 0.0 6 0.0 9.001714376503005E-6 0.0 5.401028625901803E-5 0.0 7 0.0 9.001714376503005E-6 0.0 5.401028625901803E-5 0.0 8 9.001714376503005E-6 9.001714376503005E-6 0.0 5.401028625901803E-5 0.0 9 9.001714376503005E-6 9.001714376503005E-6 0.0 8.101542938852705E-5 0.0 10-11 9.001714376503005E-6 9.001714376503005E-6 0.0 8.101542938852705E-5 0.0 12-13 9.001714376503005E-6 9.001714376503005E-6 0.0 8.551628657677856E-5 4.500857188251502E-6 14-15 9.001714376503005E-6 9.001714376503005E-6 0.0 1.1252142970628756E-4 9.001714376503005E-6 16-17 9.001714376503005E-6 9.001714376503005E-6 0.0 1.4852828721229958E-4 9.001714376503005E-6 18-19 9.001714376503005E-6 9.001714376503005E-6 0.0 1.755334303418086E-4 9.001714376503005E-6 20-21 9.001714376503005E-6 9.001714376503005E-6 0.0 2.2954371660082663E-4 9.001714376503005E-6 22-23 9.001714376503005E-6 9.001714376503005E-6 0.0 4.3658314726039576E-4 9.001714376503005E-6 24-25 9.001714376503005E-6 9.001714376503005E-6 0.0 8.011525795087674E-4 9.001714376503005E-6 26-27 9.001714376503005E-6 9.001714376503005E-6 0.0 0.001638312016523547 9.001714376503005E-6 28-29 9.001714376503005E-6 9.001714376503005E-6 0.0 0.005027457479276928 9.001714376503005E-6 30-31 9.001714376503005E-6 9.001714376503005E-6 0.0 0.018953109619727078 9.001714376503005E-6 32-33 9.001714376503005E-6 9.001714376503005E-6 0.0 0.05055362793844088 9.001714376503005E-6 34-35 9.001714376503005E-6 9.001714376503005E-6 0.0 0.09853726642239014 9.001714376503005E-6 36-37 9.001714376503005E-6 9.001714376503005E-6 0.0 0.17878755008891445 9.001714376503005E-6 38-39 9.001714376503005E-6 9.001714376503005E-6 0.0 0.2953642521218166 9.001714376503005E-6 40-41 9.001714376503005E-6 9.001714376503005E-6 0.0 0.41807562250230557 9.001714376503005E-6 42-43 9.001714376503005E-6 9.001714376503005E-6 0.0 0.537717408280407 9.001714376503005E-6 44-45 9.001714376503005E-6 9.001714376503005E-6 0.0 0.6767038782536134 9.001714376503005E-6 46-47 9.001714376503005E-6 9.001714376503005E-6 0.0 0.8435956627939791 9.001714376503005E-6 48-49 9.001714376503005E-6 9.001714376503005E-6 0.0 1.026848063213639 9.001714376503005E-6 50-51 9.001714376503005E-6 9.001714376503005E-6 0.0 1.2169642708453825 9.001714376503005E-6 52-53 9.001714376503005E-6 9.001714376503005E-6 0.0 1.3971560883770313 9.001714376503005E-6 54-55 9.001714376503005E-6 9.001714376503005E-6 0.0 1.5894822168882063 9.001714376503005E-6 56-57 9.001714376503005E-6 9.001714376503005E-6 0.0 1.8150156697343007 9.001714376503005E-6 58-59 9.001714376503005E-6 9.001714376503005E-6 0.0 2.0758583472222285 9.001714376503005E-6 60-61 9.001714376503005E-6 9.001714376503005E-6 0.0 2.337700215005948 9.001714376503005E-6 62-63 9.001714376503005E-6 9.001714376503005E-6 0.0 2.6000686830806927 9.001714376503005E-6 64-65 9.001714376503005E-6 9.001714376503005E-6 0.0 2.8666409517692646 9.001714376503005E-6 66-67 9.001714376503005E-6 9.001714376503005E-6 0.0 3.1623652724661415 9.001714376503005E-6 68-69 9.001714376503005E-6 9.001714376503005E-6 0.0 3.4891320051903887 9.001714376503005E-6 70-71 9.001714376503005E-6 9.001714376503005E-6 0.0 3.840851490166302 9.001714376503005E-6 72-73 1.3502571564754507E-5 9.001714376503005E-6 0.0 4.194812402021965 9.001714376503005E-6 74-75 1.800342875300601E-5 9.001714376503005E-6 0.0 4.5324262005698985 9.001714376503005E-6 76-77 1.800342875300601E-5 9.001714376503005E-6 0.0 4.911758444395735 9.001714376503005E-6 78-79 1.800342875300601E-5 9.001714376503005E-6 0.0 5.322173607963636 9.001714376503005E-6 80-81 1.800342875300601E-5 9.001714376503005E-6 0.0 5.756344295771129 9.001714376503005E-6 82-83 1.800342875300601E-5 2.2504285941257512E-5 0.0 6.1981349347983326 9.001714376503005E-6 84-85 1.800342875300601E-5 2.7005143129509014E-5 0.0 6.616003517869979 9.001714376503005E-6 86-87 1.800342875300601E-5 2.7005143129509014E-5 0.0 7.056259364595987 9.001714376503005E-6 88 1.800342875300601E-5 2.7005143129509014E-5 0.0 7.4144150753511004 9.001714376503005E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22055 0.0 46.927475 1 GTATCAA 38815 0.0 39.42141 1 GGGCACC 12320 0.0 36.55607 94 CAACGCA 44085 0.0 33.189575 5 ATCAACG 44830 0.0 32.68182 3 GTGGTAT 6890 0.0 32.213432 1 CTGTGCG 15710 0.0 31.015196 9 TAGGCAT 14145 0.0 30.759691 5 TATCAAC 49995 0.0 30.449728 2 TACCTGG 4770 0.0 30.249561 2 AACGCAG 50595 0.0 29.9128 6 TAGTACT 16815 0.0 29.295614 4 ACTGTGC 17685 0.0 28.826813 8 CTAGGCA 16175 0.0 28.07185 4 TCAACGC 53530 0.0 27.721424 4 TGAACCG 1410 0.0 27.658773 5 GTACTGT 18475 0.0 26.98391 6 TACTGTG 20160 0.0 26.77956 7 ACGCAGA 54675 0.0 26.692337 7 CTAACGC 5480 0.0 26.675835 3 >>END_MODULE