FastQCFastQC Report
Mon 6 Jun 2016
ERR1211260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11312324
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT386760.34189261198671467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302920.26777875174013754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179950.1590742980841072No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA223400.047.3364141
GTATCAA404750.040.4755331
GTACTAG126100.035.75411
GTGGTAT61150.035.556661
GGGCACC117700.035.01877294
CAACGCA446400.034.803515
ATCAACG454350.034.2150733
GCATCGT121500.033.464988
TATCAAC506150.032.1622732
TAGGCAT154850.031.2681185
AACGCAG516000.030.993046
CTGTGCG158450.030.4967529
TAGTACT168500.028.8186914
ACTGTGC174500.028.796068
CTAGGCA171900.028.7682674
TCAACGC552400.028.5674514
ACGCAGA547200.028.3925887
TATCCGC4500.028.237531
CGCAGAG550150.028.2299258
TACCTGG45250.027.2494742
Produced by FastQC (version 0.11.5)