FastQCFastQC Report
Mon 6 Jun 2016
ERR1211260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11312324
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352710.3117926961780798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294180.2600526646867611No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183560.1622655079539801No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA230300.047.7117161
GTATCAA406600.039.5590551
GGGCACC121250.037.14150294
GTACTAG121200.034.3709341
GTGGTAT64400.034.1680531
CAACGCA456100.033.3138775
ATCAACG464550.032.7498363
TAGGCAT146050.031.8155925
TATCAAC516200.030.9173642
CTGTGCG170350.030.0077849
AACGCAG526300.029.8969636
ACTGTGC185550.028.7143698
TAGTACT181600.028.5468734
CTAGGCA166450.028.4909064
TACCCCG6850.027.4356165
TCAACGC563350.027.4066494
ACGCAGA562100.027.0483137
CGCAGAG562550.027.0351478
GCATCGT146400.026.9257648
GTACTGT197800.026.6033926
Produced by FastQC (version 0.11.5)