##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211260_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11312324 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.096779052650895 33.0 31.0 34.0 30.0 34.0 2 32.24724353722542 33.0 31.0 34.0 30.0 34.0 3 32.3204616487293 34.0 31.0 34.0 30.0 34.0 4 35.94731798700249 37.0 35.0 37.0 35.0 37.0 5 35.83569096854015 37.0 35.0 37.0 35.0 37.0 6 35.863301121856125 37.0 35.0 37.0 35.0 37.0 7 35.8030440075797 37.0 35.0 37.0 35.0 37.0 8 35.794672253022455 37.0 35.0 37.0 33.0 37.0 9 37.37573605565046 39.0 37.0 39.0 34.0 39.0 10-11 37.35522833327617 39.0 37.0 39.0 34.0 39.0 12-13 37.24770931242776 39.0 37.0 39.0 33.5 39.0 14-15 38.428357117423445 40.0 38.0 41.0 33.5 41.0 16-17 38.39152940633596 40.0 38.0 41.0 34.0 41.0 18-19 38.42671810849831 40.0 38.0 41.0 34.0 41.0 20-21 38.39112595254521 40.0 38.0 41.0 34.0 41.0 22-23 38.252708550426945 40.0 38.0 41.0 34.0 41.0 24-25 38.17799914500327 40.0 37.5 41.0 33.0 41.0 26-27 38.02104412850976 40.0 37.0 41.0 33.0 41.0 28-29 38.011845090363394 40.0 37.0 41.0 33.0 41.0 30-31 38.018620400193626 40.0 37.0 41.0 33.0 41.0 32-33 37.88408650600884 40.0 37.0 41.0 33.0 41.0 34-35 37.742768550476455 40.0 37.0 41.0 33.0 41.0 36-37 37.602970264995946 40.0 36.0 41.0 33.0 41.0 38-39 37.500453443518765 40.0 36.0 41.0 32.0 41.0 40-41 37.387086729481936 39.5 36.0 41.0 32.0 41.0 42-43 37.225523773894736 39.0 36.0 41.0 31.5 41.0 44-45 37.037733095339206 39.0 35.5 41.0 31.0 41.0 46-47 36.76070562512177 39.0 35.0 41.0 31.0 41.0 48-49 36.47330199347189 39.0 35.0 40.5 30.0 41.0 50-51 36.23656080748748 39.0 35.0 40.0 29.5 41.0 52-53 36.00362034361817 38.0 35.0 40.0 29.0 41.0 54-55 35.7434665944858 38.0 35.0 40.0 29.0 41.0 56-57 35.44761903036016 37.5 34.0 40.0 28.0 41.0 58-59 35.137130045072965 37.0 34.0 40.0 28.0 41.0 60-61 34.86842416288643 36.5 34.0 40.0 27.0 41.0 62-63 34.55360366269566 36.0 33.5 39.0 26.5 41.0 64-65 34.178797654664066 35.5 33.0 39.0 26.0 40.5 66-67 33.88582231202005 35.0 33.0 38.5 26.0 40.0 68-69 33.580325227601335 35.0 33.0 38.0 26.0 40.0 70-71 33.319858147627315 35.0 32.0 37.5 25.5 40.0 72-73 33.02453045899322 35.0 32.0 37.0 25.0 39.5 74-75 32.716636784802134 35.0 32.0 36.0 25.0 39.0 76-77 31.759796749103014 34.0 30.5 35.0 23.5 38.0 78-79 32.291768473038786 34.5 31.5 36.0 25.0 38.0 80-81 32.249984574345646 35.0 32.0 35.0 25.0 37.0 82-83 32.02322175354949 34.5 31.5 35.0 24.5 37.0 84-85 31.79154062419004 34.0 31.0 35.0 24.0 36.5 86-87 31.56708206023802 34.0 31.0 35.0 24.0 36.0 88-89 31.360080298265856 34.0 31.0 35.0 23.5 36.0 90-91 31.146771830439086 34.0 31.0 35.0 23.0 35.0 92-93 30.954967785576155 34.0 30.5 35.0 21.5 35.0 94-95 30.77249555440597 34.0 30.0 35.0 20.0 35.0 96-97 30.602688802053407 34.0 30.0 35.0 20.0 35.0 98-99 30.43018728954369 34.0 29.5 35.0 20.0 35.0 100 28.57718970920564 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3552608836220301 1101 2 -0.33972331810515755 1101 3 -0.3918426986348784 1101 4 -0.24183373994038249 1101 5 -0.28302271359125797 1101 6 -0.3354955302029694 1101 7 -0.3199067567079297 1101 8 -0.3848043991270558 1101 9 -0.4752776287463405 1101 10-11 -0.4819641051063783 1101 12-13 -0.5010801446840034 1101 14-15 -0.7463446210318878 1101 16-17 -0.657826347411401 1101 18-19 -0.7272136847069319 1101 20-21 -0.7608885493842124 1101 22-23 -0.7644451793265716 1101 24-25 -0.73507361313861 1101 26-27 -0.7812900558001488 1101 28-29 -0.8294568817530532 1101 30-31 -0.7293628426488326 1101 32-33 -0.7070195504092425 1101 34-35 -0.7071158364378576 1101 36-37 -0.6546367204195676 1101 38-39 -0.7381567436204151 1101 40-41 -0.7712630942719798 1101 42-43 -0.7770414411071798 1101 44-45 -0.8118590451772008 1101 46-47 -0.8512068482221267 1101 48-49 -0.7779648544409596 1101 50-51 -0.7614213527753719 1101 52-53 -0.8180934712126557 1101 54-55 -0.7133923930130166 1101 56-57 -0.7564674848889226 1101 58-59 -0.818978540988148 1101 60-61 -0.865634075853599 1101 62-63 -0.9572747616533164 1101 64-65 -0.837958417199367 1101 66-67 -0.9186583988405914 1101 68-69 -0.9768351401756306 1101 70-71 -1.1013801555666518 1101 72-73 -1.1303039815315579 1101 74-75 -1.1601550067825528 1101 76-77 -1.078985836985705 1101 78-79 -0.9306995839489147 1101 80-81 -0.9091659068908484 1101 82-83 -0.9239228464433111 1101 84-85 -0.7376855638300093 1101 86-87 -0.8973440405227393 1101 88-89 -0.9227489909566167 1101 90-91 -1.0055380317919784 1101 92-93 -1.0224993797591857 1101 94-95 -1.0134555900589142 1101 96-97 -1.1561772820107947 1101 98-99 -1.265072169334708 1101 100 -1.2014466054428645 1102 1 -0.4079530060131873 1102 2 -0.3609142490952948 1102 3 -0.38319922457477773 1102 4 -0.2543878295467934 1102 5 -0.300180820667201 1102 6 -0.3288301082511609 1102 7 -0.3396415279915743 1102 8 -0.37252057802653127 1102 9 -0.49572904243341753 1102 10-11 -0.5165840906137262 1102 12-13 -0.528190582662603 1102 14-15 -0.678068581575296 1102 16-17 -0.692093573764538 1102 18-19 -0.6912731176653253 1102 20-21 -0.7048575272600672 1102 22-23 -0.689665173973431 1102 24-25 -0.7638835047048005 1102 26-27 -0.8391629251507808 1102 28-29 -0.7631007148655229 1102 30-31 -0.7209384558123517 1102 32-33 -0.6704379116942576 1102 34-35 -0.7571168417844731 1102 36-37 -0.8272630905307423 1102 38-39 -0.8102181089333413 1102 40-41 -0.7908142396490234 1102 42-43 -0.7417436953031924 1102 44-45 -0.8253458092014654 1102 46-47 -0.8151139646399557 1102 48-49 -0.7949853987643323 1102 50-51 -0.788525732561375 1102 52-53 -0.8806289031526475 1102 54-55 -0.8449217863450897 1102 56-57 -0.8556850118668109 1102 58-59 -0.9666013662732738 1102 60-61 -0.924273489357887 1102 62-63 -0.8345242116503826 1102 64-65 -0.8041522401930763 1102 66-67 -0.9163902063101901 1102 68-69 -0.9958236570282466 1102 70-71 -1.0946651974452095 1102 72-73 -1.052502087040729 1102 74-75 -1.0948467505366715 1102 76-77 -0.9355598768470443 1102 78-79 -0.8069598512144367 1102 80-81 -0.7630820192024288 1102 82-83 -0.6305561495292018 1102 84-85 -0.8076676112118655 1102 86-87 -0.7634819035736378 1102 88-89 -0.8174018264910501 1102 90-91 -0.8826569956321428 1102 92-93 -0.9711064002121113 1102 94-95 -1.0155808928053354 1102 96-97 -1.1341505554585467 1102 98-99 -1.1014712005461504 1102 100 -1.1823343134484254 1103 1 -0.3726257892656335 1103 2 -0.3892458120180464 1103 3 -0.4334443521560196 1103 4 -0.23656951733732257 1103 5 -0.2943429228002046 1103 6 -0.3690029310435534 1103 7 -0.426790570671983 1103 8 -0.40990494881912554 1103 9 -0.5642932008854018 1103 10-11 -0.5562904878560246 1103 12-13 -0.5934927051229621 1103 14-15 -0.7709316654863159 1103 16-17 -0.7424129720650683 1103 18-19 -0.7299317514503159 1103 20-21 -0.7593003640594631 1103 22-23 -0.7737439293080541 1103 24-25 -0.8701585906916165 1103 26-27 -0.8028358758192056 1103 28-29 -0.8855130362792494 1103 30-31 -0.7782130725514875 1103 32-33 -0.7525076601698615 1103 34-35 -0.8393930481705496 1103 36-37 -0.8259458567659195 1103 38-39 -0.8414052311029394 1103 40-41 -0.8847662971048962 1103 42-43 -0.8602738675062298 1103 44-45 -0.9207202837376371 1103 46-47 -0.94665436571794 1103 48-49 -1.069076970878875 1103 50-51 -1.163806028565709 1103 52-53 -1.0469588732089292 1103 54-55 -1.0562959968791361 1103 56-57 -1.0665232196143464 1103 58-59 -1.056611230133086 1103 60-61 -1.1336579649866891 1103 62-63 -1.0015006045885642 1103 64-65 -1.0374263172571503 1103 66-67 -1.0888760136808031 1103 68-69 -1.0989780749287164 1103 70-71 -1.2201823256896702 1103 72-73 -1.1074090403777461 1103 74-75 -1.1150227053359671 1103 76-77 -1.0053181167014245 1103 78-79 -0.9093197877251455 1103 80-81 -0.8532940449015882 1103 82-83 -0.8973281319819151 1103 84-85 -0.9702497069178655 1103 86-87 -0.9578883887696996 1103 88-89 -0.9922755149568729 1103 90-91 -1.0497408054403436 1103 92-93 -1.2136781259871512 1103 94-95 -1.1797968386945605 1103 96-97 -1.168630958230807 1103 98-99 -1.2274198927798388 1103 100 -1.2863832649152975 1104 1 -0.4415963076384237 1104 2 -0.4051284194394569 1104 3 -0.4323959375028146 1104 4 -0.3158769214645929 1104 5 -0.3282117094800583 1104 6 -0.3646566176286896 1104 7 -0.31627680000104874 1104 8 -0.42905338400870363 1104 9 -0.508637910164289 1104 10-11 -0.5498067433742548 1104 12-13 -0.5985973871127825 1104 14-15 -0.7506686118102195 1104 16-17 -0.7501438404177989 1104 18-19 -0.8282405254252865 1104 20-21 -0.8011997880949124 1104 22-23 -0.7676101319374808 1104 24-25 -0.790759751986009 1104 26-27 -0.8684974239996137 1104 28-29 -0.9839552928170221 1104 30-31 -0.9112703673325058 1104 32-33 -0.8515402496932722 1104 34-35 -0.8388525219654781 1104 36-37 -0.8032221995985438 1104 38-39 -0.8495027563166886 1104 40-41 -0.9367066293439734 1104 42-43 -0.8876386527970794 1104 44-45 -1.0014930547488632 1104 46-47 -0.9893702613531019 1104 48-49 -0.9158132499072238 1104 50-51 -0.8978832982011866 1104 52-53 -0.8823340663091486 1104 54-55 -0.9732064532581646 1104 56-57 -0.8760389413586722 1104 58-59 -0.9226917513490775 1104 60-61 -0.8842470374122584 1104 62-63 -0.9222170045259332 1104 64-65 -0.9868082050453282 1104 66-67 -0.9763458159382026 1104 68-69 -0.9911458868614389 1104 70-71 -1.0340647447892692 1104 72-73 -1.0772682184237894 1104 74-75 -1.0159512729730835 1104 76-77 -0.9518806983691057 1104 78-79 -0.8374646877179792 1104 80-81 -0.8152495308316645 1104 82-83 -0.811602249114955 1104 84-85 -0.8816408352172402 1104 86-87 -0.9078640296677598 1104 88-89 -0.9966595083689711 1104 90-91 -0.9707924264111583 1104 92-93 -1.010611191146264 1104 94-95 -1.0146565213555014 1104 96-97 -1.0332160936438868 1104 98-99 -1.0771661156532737 1104 100 -1.140069138660884 1105 1 -0.4081949887995222 1105 2 -0.4067664460654967 1105 3 -0.4655486102743751 1105 4 -0.2428391993159167 1105 5 -0.2877054195041282 1105 6 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1105 68-69 -0.9493937445620659 1105 70-71 -1.0842013877554493 1105 72-73 -1.0411739829944402 1105 74-75 -1.0894639833432542 1105 76-77 -0.9930882165653543 1105 78-79 -0.9701992932165773 1105 80-81 -0.8955840377708029 1105 82-83 -0.8739348420718791 1105 84-85 -0.9096413380938664 1105 86-87 -1.0395358451899455 1105 88-89 -0.9833609278240587 1105 90-91 -0.9109959075650629 1105 92-93 -0.9745634023391894 1105 94-95 -0.8714292016429681 1105 96-97 -1.0778474157476694 1105 98-99 -1.1811658576770192 1105 100 -1.1926910504883068 1106 1 -0.43894239484087194 1106 2 -0.42869221706796523 1106 3 -0.46844266274244717 1106 4 -0.30738307359494854 1106 5 -0.3162070753082915 1106 6 -0.3710276699081092 1106 7 -0.4580770589172829 1106 8 -0.4587959865312712 1106 9 -0.5406155904004422 1106 10-11 -0.5450607467057083 1106 12-13 -0.6219542089609504 1106 14-15 -0.7754910646082465 1106 16-17 -0.7770515576648336 1106 18-19 -0.7167959263121517 1106 20-21 -0.7876722729043308 1106 22-23 -0.738843757396296 1106 24-25 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0.7163687475986862 2310 14-15 0.9550538928745382 2310 16-17 0.7758569129878339 2310 18-19 0.844753617318581 2310 20-21 0.9311049110208884 2310 22-23 0.9829366527389709 2310 24-25 1.0318305935439156 2310 26-27 1.0557605869010658 2310 28-29 1.0631929072764805 2310 30-31 0.9070295330450122 2310 32-33 1.0277249256889291 2310 34-35 1.0496249586907354 2310 36-37 0.9867535308849256 2310 38-39 1.0791757869482765 2310 40-41 1.1092775187660848 2310 42-43 1.1782277102139531 2310 44-45 1.139913968225045 2310 46-47 0.7773992586630456 2310 48-49 1.050278383245903 2310 50-51 1.0878100294482849 2310 52-53 1.1050067666798 2310 54-55 1.1402166410261643 2310 56-57 1.1838251326133076 2310 58-59 1.20666103726672 2310 60-61 1.1964815222550058 2310 62-63 0.6137074894162211 2310 64-65 0.7870914423168287 2310 66-67 1.1382603359240093 2310 68-69 1.235466640591305 2310 70-71 1.310243269497228 2310 72-73 1.321394688032889 2310 74-75 1.3319887468274416 2310 76-77 1.2475116029782711 2310 78-79 1.123373095620721 2310 80-81 1.1005711583190916 2310 82-83 1.1245117429229943 2310 84-85 1.1467193624037542 2310 86-87 1.199356616373695 2310 88-89 1.1970774160842126 2310 90-91 1.2387009571161656 2310 92-93 1.2755880432684101 2310 94-95 1.3172117618946437 2310 96-97 1.3692910332603354 2310 98-99 1.3986039053419823 2310 100 1.5332199045867974 2311 1 0.41930055955445766 2311 2 0.35624036447639185 2311 3 0.43921272073902884 2311 4 0.3198370407885207 2311 5 0.4240971586656954 2311 6 0.4817190431951488 2311 7 0.5386298596815351 2311 8 0.5240538866619175 2311 9 0.7022490918489055 2311 10-11 0.713486236930116 2311 12-13 0.7494465690831973 2311 14-15 0.9054161436315908 2311 16-17 0.7776187545850206 2311 18-19 0.9058732041704687 2311 20-21 0.7909592514474397 2311 22-23 0.9810058942866249 2311 24-25 0.9918233369668812 2311 26-27 0.3445121356134848 2311 28-29 0.6739876466872303 2311 30-31 0.929300740340743 2311 32-33 1.0242378114471293 2311 34-35 1.0744266081793512 2311 36-37 1.103329281747854 2311 38-39 1.1189321117351696 2311 40-41 1.1281936349168546 2311 42-43 1.0894828691866891 2311 44-45 1.0936851474206009 2311 46-47 0.7691242458362026 2311 48-49 1.0869508415859954 2311 50-51 1.1981717462128145 2311 52-53 1.107262372573281 2311 54-55 1.117537331728812 2311 56-57 1.2346925128870794 2311 58-59 1.231612508609821 2311 60-61 1.2575343808999762 2311 62-63 1.232748810723514 2311 64-65 1.2930420500342947 2311 66-67 1.2904901063531113 2311 68-69 1.3465329414254086 2311 70-71 1.3272577285964715 2311 72-73 1.3714655522980124 2311 74-75 1.3749261562878488 2311 76-77 1.328944626160876 2311 78-79 1.0482413153832084 2311 80-81 1.020861676477196 2311 82-83 1.1140551262698573 2311 84-85 1.1866473991629398 2311 86-87 1.2606092692765891 2311 88-89 1.2449215732255823 2311 90-91 1.2929606947768377 2311 92-93 1.3854677870783512 2311 94-95 1.3439464464555435 2311 96-97 1.4030341641407702 2311 98-99 1.4565286375456274 2311 100 1.4846651619196436 2312 1 0.3151395214111474 2312 2 0.42464577778814316 2312 3 0.4792501700897702 2312 4 0.22336659620703614 2312 5 0.3818580077141078 2312 6 0.43688254718810526 2312 7 0.34287382993143467 2312 8 0.4638942831035209 2312 9 0.6489133052484988 2312 10-11 0.6626915404670015 2312 12-13 0.7129983003800788 2312 14-15 0.9207557761904752 2312 16-17 0.8859339000503184 2312 18-19 0.8422173283747796 2312 20-21 0.8533914975559682 2312 22-23 0.9789064363360893 2312 24-25 0.9698352948622073 2312 26-27 0.9885886920977569 2312 28-29 0.9824318734004365 2312 30-31 0.9823241186518672 2312 32-33 0.8670622862430619 2312 34-35 0.9823095076850734 2312 36-37 1.0269635182338064 2312 38-39 0.8329616918824811 2312 40-41 0.9693728416943799 2312 42-43 1.076059590391118 2312 44-45 0.8161970835375172 2312 46-47 0.8354642251252073 2312 48-49 0.9941275541042671 2312 50-51 0.8335482142136712 2312 52-53 1.0119624937572453 2312 54-55 0.8764789204933905 2312 56-57 0.9198334065793148 2312 58-59 0.9989660022155462 2312 60-61 1.0660647616522496 2312 62-63 1.0621173949695404 2312 64-65 1.0704535271386888 2312 66-67 1.0757506526722125 2312 68-69 1.1080081870815732 2312 70-71 1.0806471898191816 2312 72-73 1.1016751457085192 2312 74-75 1.1109612115777594 2312 76-77 1.0893244462834275 2312 78-79 1.01820533394811 2312 80-81 0.9717850967049486 2312 82-83 1.0052390115522876 2312 84-85 1.0729522222646715 2312 86-87 1.0495524059322356 2312 88-89 1.0745074889625243 2312 90-91 1.1595112563415029 2312 92-93 1.2160778207501188 2312 94-95 1.1999993372957896 2312 96-97 0.8669355673467898 2312 98-99 0.6801626586250045 2312 100 0.9824083053491428 2313 1 0.0490500120593893 2313 2 0.22355728159363508 2313 3 0.2908388300335645 2313 4 0.18397750318286654 2313 5 0.30934595481308946 2313 6 0.29236601115301397 2313 7 0.3233944304559131 2313 8 0.43193071405389105 2313 9 0.6228673930030482 2313 10-11 0.43348913962218916 2313 12-13 0.5006615987271275 2313 14-15 0.5885829664170998 2313 16-17 0.7830719641630921 2313 18-19 0.7491199123985766 2313 20-21 0.7208137813596096 2313 22-23 0.671307460995024 2313 24-25 0.5795254408741712 2313 26-27 0.8131092536651181 2313 28-29 0.602602123797503 2313 30-31 0.8376349630314408 2313 32-33 0.9170778921352181 2313 34-35 0.9900811786822459 2313 36-37 1.0239455656248069 2313 38-39 1.0121777629790927 2313 40-41 1.0077075854187925 2313 42-43 1.09404605089221 2313 44-45 0.8997305593630784 2313 46-47 0.8949900052311648 2313 48-49 0.9884793322189793 2313 50-51 0.9949396917624185 2313 52-53 1.0710059879125922 2313 54-55 1.0023438348180704 2313 56-57 0.6897169986896046 2313 58-59 0.7355709503286434 2313 60-61 0.6585333693664026 2313 62-63 0.9910414472882749 2313 64-65 1.1317876373267879 2313 66-67 1.0493384207666594 2313 68-69 1.1407502572458341 2313 70-71 1.0876822846946297 2313 72-73 1.0926029503821297 2313 74-75 1.209517985689068 2313 76-77 1.1458946212782593 2313 78-79 1.0012322361452277 2313 80-81 0.8950205790871522 2313 82-83 0.9962391834111592 2313 84-85 0.9499288485300319 2313 86-87 0.9844071192712001 2313 88-89 0.9912129558899778 2313 90-91 0.9030745927789177 2313 92-93 1.13778109608959 2313 94-95 1.208401615601126 2313 96-97 1.2820727796778577 2313 98-99 1.2701169855638454 2313 100 1.441192061666257 2314 1 -0.33706570461673735 2314 2 0.055083361510632756 2314 3 0.32799557753927644 2314 4 0.2744043729263552 2314 5 0.3644113672192688 2314 6 0.39469582687592464 2314 7 0.44985650654723486 2314 8 0.5008499887128224 2314 9 0.456927609462646 2314 10-11 0.6079398461526395 2314 12-13 0.525443381195366 2314 14-15 0.8299740225649188 2314 16-17 0.855012420778678 2314 18-19 0.8873167807858167 2314 20-21 0.9213933500446814 2314 22-23 0.9486014118215138 2314 24-25 0.8838876032541805 2314 26-27 0.8271284451405805 2314 28-29 0.8435239600795299 2314 30-31 0.9005118541237493 2314 32-33 0.949280021622549 2314 34-35 0.9359744461049075 2314 36-37 0.9187057045813134 2314 38-39 0.6776454488482244 2314 40-41 0.7122221333543806 2314 42-43 0.8714740849929825 2314 44-45 0.940047165358024 2314 46-47 0.8723988728667038 2314 48-49 0.8749737596729545 2314 50-51 0.8556779763889963 2314 52-53 0.8665429175998369 2314 54-55 0.7882461665699267 2314 56-57 0.7859224372545555 2314 58-59 0.9084544320727943 2314 60-61 0.9599259172562782 2314 62-63 0.8644165435592939 2314 64-65 0.9109959071638016 2314 66-67 0.5814196346945266 2314 68-69 0.543337493651272 2314 70-71 0.7607317975652634 2314 72-73 0.925018353612181 2314 74-75 1.0572913411174412 2314 76-77 0.8493870064000149 2314 78-79 0.7479286375895811 2314 80-81 0.8457892111691763 2314 82-83 0.8408616001434659 2314 84-85 0.9005616032717292 2314 86-87 0.9153416184026888 2314 88-89 0.8944111883008645 2314 90-91 0.974051548890543 2314 92-93 0.9000050212613928 2314 94-95 0.9507647607474894 2314 96-97 0.9949089920772849 2314 98-99 1.084083019339598 2314 100 1.0855952558809712 2315 1 0.43015690300571663 2315 2 0.4511651306967508 2315 3 0.4961797421839762 2315 4 0.3326646639907409 2315 5 0.3749820719025081 2315 6 0.44347190999192776 2315 7 0.4021955472378451 2315 8 0.3107978080598599 2315 9 0.554262236804071 2315 10-11 0.613371875983141 2315 12-13 0.4130365844689905 2315 14-15 0.6323281405380285 2315 16-17 0.8051112910577487 2315 18-19 0.8200981229473356 2315 20-21 0.5950336492662203 2315 22-23 0.8901145425866801 2315 24-25 0.9117465510439757 2315 26-27 0.8375358090159821 2315 28-29 0.8620427429672972 2315 30-31 0.899652145025712 2315 32-33 0.8528010966633062 2315 34-35 0.8867261156674147 2315 36-37 0.8980192105362121 2315 38-39 0.9439319055717874 2315 40-41 0.9521147951552607 2315 42-43 1.0455051867112601 2315 44-45 1.054364690220794 2315 46-47 1.0224190477015256 2315 48-49 0.9458768263778197 2315 50-51 0.9771964316820814 2315 52-53 1.0155508649583354 2315 54-55 0.8731831503286216 2315 56-57 0.8170458379908325 2315 58-59 0.8634612795286998 2315 60-61 0.9889886387837521 2315 62-63 1.0184173932838192 2315 64-65 1.1142971021243753 2315 66-67 1.1268708069591469 2315 68-69 1.143900046225319 2315 70-71 1.070174486054853 2315 72-73 1.1618125269750763 2315 74-75 0.49441814923793004 2315 76-77 0.5339153948358373 2315 78-79 0.8249366932046058 2315 80-81 0.8844917150840814 2315 82-83 0.7608248191363991 2315 84-85 0.841871967896946 2315 86-87 0.9174055819368938 2315 88-89 0.9171400601013104 2315 90-91 0.9574478016272607 2315 92-93 0.9836508623188003 2315 94-95 1.0377982129256011 2315 96-97 1.2215157627460513 2315 98-99 1.250337464229581 2315 100 1.1337747733797876 2316 1 0.3141840168507315 2316 2 0.12402585297054713 2316 3 0.27644122718061936 2316 4 0.04036542719253333 2316 5 0.298282860009337 2316 6 0.37313644994040374 2316 7 0.4497193618235542 2316 8 0.4567791448012386 2316 9 0.6335211651359529 2316 10-11 0.6476355854786178 2316 12-13 0.39124266077882197 2316 14-15 0.445392188463849 2316 16-17 0.7534408009012736 2316 18-19 0.7358593545069141 2316 20-21 0.7192489300100178 2316 22-23 0.7848007680503457 2316 24-25 0.4840789802773102 2316 26-27 0.7262524568449393 2316 28-29 0.9069509414898675 2316 30-31 0.6215847745927903 2316 32-33 0.724604025962563 2316 34-35 0.7964185690835492 2316 36-37 0.8508945609115202 2316 38-39 0.7826257008332149 2316 40-41 0.7660659257761537 2316 42-43 0.7468132389824902 2316 44-45 0.5891715283295085 2316 46-47 0.6980251191541171 2316 48-49 0.7574463182944768 2316 50-51 0.79072957340437 2316 52-53 0.8500602149188765 2316 54-55 0.7153515435418925 2316 56-57 0.850175512531365 2316 58-59 0.7360237570941521 2316 60-61 0.8430638427296984 2316 62-63 0.836242742449052 2316 64-65 0.8830364459107827 2316 66-67 0.8832900557741539 2316 68-69 0.8760963416743976 2316 70-71 0.8655836195967908 2316 72-73 0.8780557922650445 2316 74-75 0.8466138256639724 2316 76-77 0.6804368562015313 2316 78-79 0.4854590475655982 2316 80-81 0.4728902346590047 2316 82-83 0.3742611465873402 2316 84-85 0.5085650135068498 2316 86-87 0.5808183723134768 2316 88-89 0.5612852549757399 2316 90-91 0.44466965927366076 2316 92-93 0.2883515190087884 2316 94-95 0.3418730544855819 2316 96-97 0.6280162262822024 2316 98-99 0.756684474263519 2316 100 0.4107539210132458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 3.0 11 7.0 12 37.0 13 238.0 14 1034.0 15 2934.0 16 6941.0 17 14511.0 18 25293.0 19 37893.0 20 56412.0 21 82268.0 22 98610.0 23 95860.0 24 91999.0 25 98830.0 26 116972.0 27 144630.0 28 179367.0 29 221320.0 30 274288.0 31 343049.0 32 436974.0 33 581124.0 34 842165.0 35 1162393.0 36 1810262.0 37 2478040.0 38 1813303.0 39 295548.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.226851593907895 22.064926526655277 13.891369572719233 22.816852306717585 2 15.161941967008724 20.23265953132177 41.182165574465515 23.423232927203994 3 18.58209683527452 26.948918719089022 29.253449600630248 25.21553484500621 4 12.172589554312351 17.045859526316377 39.994954038953125 30.78659688041815 5 12.783871819795825 38.79777488692863 34.650254006161774 13.768099287113772 6 29.31580637188256 38.80382138983997 17.815905909342767 14.064466328934708 7 26.92963143102187 32.38596995572406 23.008135083437956 17.676263529816115 8 26.503563723952745 35.314061018761485 19.464320505671516 18.718054751614257 9 26.129418832200745 15.46379420931907 21.98637533645033 36.42041162202985 10-11 25.90092769948138 26.47492760966751 26.704483374002987 20.919661316848128 12-13 25.86447868114804 24.10953417306356 27.788300962220536 22.23768618356786 14-15 23.257039311288352 25.231335366899625 25.646785420184496 25.864839901627523 16-17 21.41755117664063 28.01383716294402 27.014592094085284 23.55401956633007 18-19 20.831824654244343 27.93812747937559 29.69847751885466 21.531570347525406 20-21 22.55730079111963 26.970006688282034 29.0933417560073 21.379350764591038 22-23 22.84336092212352 26.73501041872563 28.762785613283352 21.6588430458675 24-25 22.154978567060997 26.773467302425217 29.69611026478261 21.375443865731174 26-27 22.09891863066478 27.05488793039244 28.58747940014732 22.25871403879546 28-29 21.209597546318577 27.414485178444576 29.253636835206397 22.12228044003045 30-31 22.27343858291773 26.948942506527562 29.157772007717092 21.619846902837615 32-33 22.66871289313648 27.218467918875216 28.200155323654602 21.9126638643337 34-35 22.508949874073522 27.108124523570133 29.08018275454077 21.30274284781558 36-37 22.09200120826174 26.695554473999383 28.74541684676228 22.467027470976607 38-39 22.28836698117295 26.79113405288692 29.34199927673281 21.578499689207316 40-41 21.941682825905033 25.818428166194487 30.00653323705343 22.23335577084705 42-43 21.993126251622787 26.036843567107493 29.20056409325272 22.769466088017005 44-45 22.092583370236675 27.05343105579387 28.9142567363809 21.939728837588557 46-47 22.149388866460942 26.927611253960333 28.15237163596278 22.77062824361595 48-49 23.042995047539975 26.79969791249151 28.119505259657174 22.03780178031134 50-51 22.561343908699673 26.594902726232878 28.024838684523633 22.818914680543813 52-53 21.89754212150498 27.236966394961982 27.795996089333887 23.069495394199148 54-55 21.9940013257696 27.143271524792407 27.722225984482947 23.140501164955054 56-57 23.01183585814933 26.690317373342136 27.32317407552759 22.974672692980942 58-59 21.830061314710093 27.22841243480533 28.170172963974572 22.771353286510013 60-61 22.025840408222244 27.81045192756807 27.405710127217386 22.7579975369923 62-63 22.465708824843198 27.026399981542 27.004410383969756 23.50348080964505 64-65 21.61141281819719 27.239547811515173 27.432011999995577 23.71702737029205 66-67 22.143591920853734 26.886808807985386 27.461229562210043 23.508369708950834 68-69 21.746911772471318 26.825572139928354 27.727479902495695 23.700036185104633 70-71 21.663576128248472 26.487199634335912 27.91541576954672 23.933808467868893 72-73 22.345383295579783 26.582795956726983 28.011078129048123 23.060742618645108 74-75 22.49219382137978 26.996458369258114 27.48037069952734 23.03097710983477 76-77 22.196284274386535 26.928941130280748 27.129271359033464 23.745503236299253 78-79 22.036337218683315 26.89450707641067 27.29707609856836 23.772079606337662 80-81 21.526161189085773 27.479302962340658 27.79548852027855 23.19904732829502 82-83 22.559821564220734 26.390434740007247 28.838731289207704 22.21101240656432 84-85 22.78972151862461 26.833462270132603 27.466443634158644 22.910372577084143 86-87 21.368713582876044 27.56365076320593 27.88937468763526 23.178260966282764 88-89 22.38686093355426 26.348887794144588 27.752508134580182 23.511743137720966 90-91 22.07006014815892 26.416595758718582 27.902310736803226 23.611033356319272 92-93 22.351987968100822 26.737154303208438 27.688879332182548 23.221978396508188 94-95 21.19156938637716 26.643575610830396 28.38803865979222 23.776816343000228 96-97 21.58278173474832 26.685220587939533 27.817108796408945 23.914888880903195 98-99 20.940611371086643 27.18955863596475 27.781420136916353 24.088409856032257 100 21.627311117016127 26.6461484588574 27.024805337151058 24.701735086975418 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 798.0 1 543.5 2 418.0 3 592.5 4 524.5 5 473.0 6 804.0 7 1600.5 8 2552.0 9 3198.5 10 3244.0 11 3335.5 12 3967.0 13 5018.5 14 6497.0 15 8175.5 16 9866.5 17 11487.5 18 13190.0 19 14801.0 20 16336.5 21 17708.5 22 19418.5 23 21995.5 24 24650.0 25 28267.5 26 34419.0 27 40867.5 28 50301.0 29 71630.0 30 101902.5 31 130819.5 32 173448.5 33 220082.5 34 253882.5 35 290968.0 36 324864.5 37 376320.5 38 399295.0 39 372984.0 40 340462.5 41 309194.5 42 299558.5 43 310595.0 44 339821.0 45 405493.5 46 461748.5 47 497938.0 48 528540.0 49 520394.5 50 492171.5 51 494412.5 52 494761.0 53 464708.5 54 437525.5 55 377056.0 56 320779.5 57 259500.0 58 199526.5 59 194056.0 60 160376.5 61 104411.5 62 74800.5 63 62765.5 64 44723.5 65 19161.5 66 9401.0 67 6283.5 68 4797.5 69 3795.0 70 2976.5 71 2230.0 72 1705.0 73 1559.0 74 1318.0 75 980.0 76 729.0 77 473.5 78 272.0 79 159.5 80 108.0 81 81.5 82 52.0 83 25.0 84 14.5 85 9.0 86 7.0 87 5.5 88 3.5 89 1.5 90 1.5 91 1.0 92 0.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04382830619066427 2 0.0 3 0.0 4 0.037746443613177985 5 0.0 6 0.0 7 7.60232822185786E-4 8 0.0 9 0.010775858258656665 10-11 3.5359666148176093E-4 12-13 4.419958268522012E-6 14-15 1.1933887325009432E-4 16-17 3.005571622594968E-4 18-19 0.0 20-21 1.7679833074088047E-5 22-23 0.0 24-25 5.569147418337735E-4 26-27 4.1105611897254707E-4 28-29 1.4143866459270438E-4 30-31 0.002581255628816855 32-33 0.015452174106752955 34-35 0.026051234034668736 36-37 0.031081146544246787 38-39 0.022400348504869556 40-41 0.001480686019954874 42-43 8.839916537044023E-5 44-45 0.010988016255545721 46-47 0.017945030570199368 48-49 0.003270769118706289 50-51 0.011120615003601382 52-53 0.01067419921848066 54-55 0.01023662334989698 56-57 4.1989603550959115E-4 58-59 0.00891947578587742 60-61 0.004813334554420471 62-63 0.0013878668963159117 64-65 0.035240327274926 66-67 0.05899760296823181 68-69 0.058051731898768104 70-71 0.05102399825181811 72-73 0.04419516272695159 74-75 0.03512982831821295 76-77 0.03090876817177443 78-79 0.015178136694104588 80-81 0.0012154885238435532 82-83 0.03781716294547433 84-85 0.05083836000454018 86-87 0.056845083291461596 88-89 0.0690132284047027 90-91 0.04783278838194521 92-93 0.038749774140132476 94-95 0.012826718895250878 96-97 0.003796744152660408 98-99 0.022090951426073015 100 0.03243365377441452 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1312324E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.67073733245707 #Duplication Level Percentage of deduplicated Percentage of total 1 75.17407036380557 47.86388488345509 2 15.195616184333621 19.35032173355079 3 4.949720196141128 9.454570034329791 4 2.081475740328823 5.301163805054324 5 1.0014171383953547 3.188048378949572 6 0.537222247680946 2.0523201972747422 7 0.31236288395667516 1.3921862595769963 8 0.1832842589971572 0.9335875129425621 9 0.13142542886959857 0.7531158564325557 >10 0.39228981494934134 3.9983942813726614 >50 0.02185892448642776 0.9747313075280347 >100 0.016899964833558778 2.1910583725157173 >500 0.0014966468434390394 0.6500646466052189 >1k 7.667057217137307E-4 0.7347986847026072 >5k 3.740026263182908E-5 0.1729777989409646 >10k+ 5.6100393947743616E-5 0.988776246768475 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35271 0.3117926961780798 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29418 0.2600526646867611 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18356 0.1622655079539801 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 8.839916537044024E-6 0.0 14-15 0.0 0.0 0.0 2.209979134261006E-5 0.0 16-17 0.0 0.0 0.0 3.5359666148176095E-5 0.0 18-19 0.0 0.0 0.0 9.281912363896225E-5 0.0 20-21 0.0 0.0 0.0 1.9889812208349054E-4 0.0 22-23 0.0 0.0 0.0 4.1547607724106914E-4 4.419958268522012E-6 24-25 0.0 0.0 0.0 8.707317788988363E-4 8.839916537044024E-6 26-27 0.0 0.0 0.0 0.0016088648097420124 8.839916537044024E-6 28-29 0.0 0.0 0.0 0.004636536223679591 8.839916537044024E-6 30-31 0.0 0.0 0.0 0.017171537873208017 8.839916537044024E-6 32-33 0.0 0.0 0.0 0.04480511696800764 8.839916537044024E-6 34-35 0.0 0.0 0.0 0.08679030056069822 1.7679833074088047E-5 36-37 0.0 0.0 0.0 0.15747427319090224 1.7679833074088047E-5 38-39 0.0 0.0 0.0 0.2606626189278171 1.7679833074088047E-5 40-41 0.0 0.0 0.0 0.37005216611546843 1.7679833074088047E-5 42-43 0.0 0.0 0.0 0.47710797533734006 1.7679833074088047E-5 44-45 0.0 0.0 0.0 0.6036867402312734 1.7679833074088047E-5 46-47 0.0 0.0 0.0 0.7542923982728925 1.7679833074088047E-5 48-49 0.0 0.0 0.0 0.9223789912665161 1.7679833074088047E-5 50-51 0.0 0.0 8.839916537044024E-6 1.098266810604081 1.7679833074088047E-5 52-53 0.0 0.0 8.839916537044024E-6 1.2623577613229608 1.7679833074088047E-5 54-55 0.0 0.0 8.839916537044024E-6 1.437825684625016 1.7679833074088047E-5 56-57 0.0 0.0 8.839916537044024E-6 1.6452808459163653 1.7679833074088047E-5 58-59 0.0 0.0 8.839916537044024E-6 1.888201752354335 1.7679833074088047E-5 60-61 0.0 0.0 8.839916537044024E-6 2.1334121971753994 1.7679833074088047E-5 62-63 0.0 0.0 8.839916537044024E-6 2.38225142773492 1.7679833074088047E-5 64-65 0.0 0.0 8.839916537044024E-6 2.630772421299107 1.7679833074088047E-5 66-67 0.0 0.0 8.839916537044024E-6 2.9099900250381796 1.7679833074088047E-5 68-69 0.0 0.0 8.839916537044024E-6 3.213619058294299 1.7679833074088047E-5 70-71 0.0 0.0 8.839916537044024E-6 3.549186709998759 1.7679833074088047E-5 72-73 0.0 0.0 8.839916537044024E-6 3.8851698377804595 2.6519749611132073E-5 74-75 0.0 0.0 8.839916537044024E-6 4.207380375597445 2.6519749611132073E-5 76-77 0.0 0.0 8.839916537044024E-6 4.572769485739624 2.6519749611132073E-5 78-79 0.0 0.0 8.839916537044024E-6 4.962729143896515 2.6519749611132073E-5 80-81 0.0 0.0 8.839916537044024E-6 5.377582007021722 2.6519749611132073E-5 82-83 0.0 0.0 8.839916537044024E-6 5.799038287800101 2.6519749611132073E-5 84-85 0.0 0.0 8.839916537044024E-6 6.199376007971483 2.6519749611132073E-5 86-87 0.0 0.0 8.839916537044024E-6 6.622370434227308 2.6519749611132073E-5 88 0.0 0.0 8.839916537044024E-6 6.962830979735021 2.6519749611132073E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 23030 0.0 47.711716 1 GTATCAA 40660 0.0 39.559055 1 GGGCACC 12125 0.0 37.141502 94 GTACTAG 12120 0.0 34.370934 1 GTGGTAT 6440 0.0 34.168053 1 CAACGCA 45610 0.0 33.313877 5 ATCAACG 46455 0.0 32.749836 3 TAGGCAT 14605 0.0 31.815592 5 TATCAAC 51620 0.0 30.917364 2 CTGTGCG 17035 0.0 30.007784 9 AACGCAG 52630 0.0 29.896963 6 ACTGTGC 18555 0.0 28.714369 8 TAGTACT 18160 0.0 28.546873 4 CTAGGCA 16645 0.0 28.490906 4 TACCCCG 685 0.0 27.435616 5 TCAACGC 56335 0.0 27.406649 4 ACGCAGA 56210 0.0 27.048313 7 CGCAGAG 56255 0.0 27.035147 8 GCATCGT 14640 0.0 26.925764 8 GTACTGT 19780 0.0 26.603392 6 >>END_MODULE