FastQCFastQC Report
Mon 6 Jun 2016
ERR1211259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8462001
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230620.2725360112814924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179120.2116757017636845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114520.13533442031027884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124800.044.6881561
GTATCAA216950.039.568891
CAACGCA247600.033.357695
ATCAACG251200.032.917063
GTACTAG52350.031.9154031
TATCAAC271650.031.201812
AACGCAG277900.030.2959426
TCAACGC282200.029.7173524
TACCTGG59050.028.612142
GCATCGT49600.028.525048
GTACATG298100.028.197341
TACATGG299050.027.351542
CTGTGCG69250.027.287469
ACGCAGA305700.026.9722187
ACATGGG299000.026.4284293
CGCAGAG314200.026.1802228
GTGGTAT51650.026.1517181
TAGTACT74900.025.418244
TAGGCAT68400.025.0161295
CTAGTAC77350.024.6740553
Produced by FastQC (version 0.11.5)