FastQCFastQC Report
Mon 6 Jun 2016
ERR1211259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8462001
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211510.24995270031284564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173190.2046679030172651No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114900.13578348667176948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131400.045.2719081
GTATCAA220400.037.9148671
GTACTAG53750.032.633531
ATCAACG259000.030.9824583
CAACGCA260300.030.6878835
TATCAAC285100.029.1325042
TACCTGG60250.028.7097422
AACGCAG290500.028.1122636
TCAACGC291300.027.7729824
GTACATG299650.026.9614071
GTGGTAT53600.026.5834581
CTGTGCG71300.026.2948349
TACATGG302400.026.2534482
ACATGGG300300.025.2969513
ACGCAGA317500.025.2776287
GTACCTG72300.025.2363831
TAGTACT78050.024.874784
CTAGTAC79900.024.7106783
CGCAGAG324800.024.6802798
GCATCGT60750.024.3641538
Produced by FastQC (version 0.11.5)