##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211259_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8462001 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17572250345988 33.0 31.0 34.0 30.0 34.0 2 32.311351180412295 34.0 31.0 34.0 31.0 34.0 3 32.392600402670716 34.0 31.0 34.0 31.0 34.0 4 35.999924367770696 37.0 35.0 37.0 35.0 37.0 5 35.8875930173017 37.0 35.0 37.0 35.0 37.0 6 35.91479946646189 37.0 35.0 37.0 35.0 37.0 7 35.851437030083076 37.0 35.0 37.0 35.0 37.0 8 35.836951331014966 37.0 35.0 37.0 35.0 37.0 9 37.45113537566351 39.0 37.0 39.0 34.0 39.0 10-11 37.42871526486466 39.0 37.0 39.0 34.0 39.0 12-13 37.333944063584966 39.0 37.0 39.0 34.0 39.0 14-15 38.57597003356535 40.0 38.0 41.0 34.0 41.0 16-17 38.528429623206144 40.0 38.0 41.0 34.0 41.0 18-19 38.54208076789402 40.0 38.0 41.0 34.0 41.0 20-21 38.50082450947477 40.0 38.0 41.0 34.0 41.0 22-23 38.374058688955486 40.0 38.0 41.0 34.0 41.0 24-25 38.31604640557239 40.0 38.0 41.0 34.0 41.0 26-27 38.18576297733834 40.0 38.0 41.0 33.5 41.0 28-29 38.163256539440255 40.0 38.0 41.0 33.5 41.0 30-31 38.18310604075797 40.0 38.0 41.0 34.0 41.0 32-33 38.062213240107155 40.0 37.0 41.0 33.0 41.0 34-35 37.92671756952049 40.0 37.0 41.0 33.0 41.0 36-37 37.78764089013934 40.0 37.0 41.0 33.0 41.0 38-39 37.702168553277176 40.0 37.0 41.0 33.0 41.0 40-41 37.60686769004164 40.0 37.0 41.0 33.0 41.0 42-43 37.46957534039525 40.0 36.5 41.0 32.0 41.0 44-45 37.3408398911794 40.0 36.0 41.0 32.0 41.0 46-47 37.12246169670743 39.5 36.0 41.0 31.0 41.0 48-49 36.90595599078752 39.0 36.0 41.0 31.0 41.0 50-51 36.7132712463636 39.0 35.0 41.0 30.5 41.0 52-53 36.51503710528987 39.0 35.0 40.5 30.0 41.0 54-55 36.28087930975191 38.5 35.0 40.0 30.0 41.0 56-57 36.02788052140386 38.0 35.0 40.0 29.0 41.0 58-59 35.76007040178794 38.0 34.5 40.0 29.0 41.0 60-61 35.49606239706188 37.5 34.0 40.0 28.0 41.0 62-63 35.19460781202933 37.0 34.0 40.0 28.0 41.0 64-65 34.81939407712195 36.5 34.0 39.5 28.0 41.0 66-67 34.51326175688233 36.0 33.5 39.0 27.0 40.5 68-69 34.18033346958952 35.5 33.0 39.0 26.0 40.0 70-71 33.85393738431371 35.0 33.0 38.0 26.0 40.0 72-73 33.512103106582 35.0 33.0 37.5 26.0 40.0 74-75 33.17346494050284 35.0 32.0 37.0 26.0 39.0 76-77 32.17911768150346 34.0 31.0 36.0 24.5 38.5 78-79 32.66978442805667 35.0 32.0 36.0 26.0 38.5 80-81 32.594137545008564 35.0 32.0 36.0 26.0 37.5 82-83 32.3405429755917 35.0 32.0 35.0 25.0 37.0 84-85 32.08966892109798 34.0 32.0 35.0 25.0 36.5 86-87 31.85290524073443 34.0 31.0 35.0 25.0 36.0 88-89 31.637260796825714 34.0 31.0 35.0 24.5 36.0 90-91 31.42676608050507 34.0 31.0 35.0 24.0 35.5 92-93 31.249097465244922 34.0 31.0 35.0 23.5 35.0 94-95 31.061701304455056 34.0 31.0 35.0 23.0 35.0 96-97 30.89274971723591 34.0 30.0 35.0 22.5 35.0 98-99 30.72077756785895 34.0 30.0 35.0 20.0 35.0 100 28.928729977696765 32.0 26.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3198098638824938 1101 2 -0.31541475081048276 1101 3 -0.37819361455309064 1101 4 -0.20953147060120614 1101 5 -0.289882663488207 1101 6 -0.38790004738176265 1101 7 -0.3612297198405585 1101 8 -0.3986876381947724 1101 9 -0.48857674602233203 1101 10-11 -0.5030475558228744 1101 12-13 -0.4935129362628743 1101 14-15 -0.6716772069127188 1101 16-17 -0.6504976296721168 1101 18-19 -0.7379043236410681 1101 20-21 -0.7397447994031623 1101 22-23 -0.7473553350825597 1101 24-25 -0.711660985227816 1101 26-27 -0.7930627809830284 1101 28-29 -0.83010666941761 1101 30-31 -0.7199810629944352 1101 32-33 -0.7010026563915304 1101 34-35 -0.777358845502846 1101 36-37 -0.7111768102086629 1101 38-39 -0.6929540474828428 1101 40-41 -0.7624203227131545 1101 42-43 -0.7192843477669797 1101 44-45 -0.7264197185406545 1101 46-47 -0.7097512830458186 1101 48-49 -0.7160529311642563 1101 50-51 -0.7758154230346719 1101 52-53 -0.8691433621938884 1101 54-55 -0.7715108913708022 1101 56-57 -0.7096189939983617 1101 58-59 -0.7626562103082932 1101 60-61 -0.8062694068155238 1101 62-63 -0.8896515156290477 1101 64-65 -0.852541817898576 1101 66-67 -0.9228545801495684 1101 68-69 -0.9552916014732986 1101 70-71 -1.063965990262112 1101 72-73 -1.1239539429816574 1101 74-75 -1.051610961071475 1101 76-77 -1.0380641860964133 1101 78-79 -0.8879084438972811 1101 80-81 -0.8119566148592767 1101 82-83 -0.7930917851786319 1101 84-85 -0.782946012097888 1101 86-87 -0.9654745995831782 1101 88-89 -0.945138138127728 1101 90-91 -0.8689491311777573 1101 92-93 -0.9729119265767032 1101 94-95 -0.9901193480403983 1101 96-97 -1.0705033650181903 1101 98-99 -1.2238692138956502 1101 100 -0.990432524172526 1102 1 -0.39723484155883426 1102 2 -0.3214855111308026 1102 3 -0.39146947891289585 1102 4 -0.2154182019556714 1102 5 -0.330500693436079 1102 6 -0.33150155727101094 1102 7 -0.33940953993165834 1102 8 -0.3964475686517659 1102 9 -0.5099968810662432 1102 10-11 -0.5064326818686169 1102 12-13 -0.5108966799431442 1102 14-15 -0.6386347426878274 1102 16-17 -0.6945375611754159 1102 18-19 -0.6842215998745758 1102 20-21 -0.749247502147611 1102 22-23 -0.7383492625998684 1102 24-25 -0.8039665587105702 1102 26-27 -0.8540154816965853 1102 28-29 -0.8322416836510413 1102 30-31 -0.644216992633325 1102 32-33 -0.6948225916098494 1102 34-35 -0.6504298583495896 1102 36-37 -0.7525337383153285 1102 38-39 -0.8805390802024462 1102 40-41 -0.8949642185331115 1102 42-43 -0.7305950504017957 1102 44-45 -0.791607394584716 1102 46-47 -0.7733951636975434 1102 48-49 -0.7725445713886998 1102 50-51 -0.816616403099168 1102 52-53 -0.7902560327783945 1102 54-55 -0.8037706031620999 1102 56-57 -0.8657783032350466 1102 58-59 -0.9930190818142819 1102 60-61 -0.9264617754751896 1102 62-63 -0.9214320385544568 1102 64-65 -0.82394389073513 1102 66-67 -0.8837384029643758 1102 68-69 -0.9837628774154155 1102 70-71 -0.9984159439037725 1102 72-73 -1.0118007802329316 1102 74-75 -1.1013890644976883 1102 76-77 -0.9347799968059931 1102 78-79 -0.692439237793657 1102 80-81 -0.7045820633529871 1102 82-83 -0.6352519032872621 1102 84-85 -0.8524813085809839 1102 86-87 -0.8385831734175575 1102 88-89 -0.8354464645308717 1102 90-91 -0.8159051997179212 1102 92-93 -0.7749514399407538 1102 94-95 -1.004602378442332 1102 96-97 -1.0957976571070418 1102 98-99 -1.1677190010614105 1102 100 -1.0649177512629073 1103 1 -0.33864120422821387 1103 2 -0.3403622985293211 1103 3 -0.3631186014031691 1103 4 -0.2261513635058705 1103 5 -0.19633401814576246 1103 6 -0.28994631001404514 1103 7 -0.37949526196135963 1103 8 -0.29200071129745453 1103 9 -0.5088721918020909 1103 10-11 -0.52708162043659 1103 12-13 -0.5095352806247107 1103 14-15 -0.6451260035529884 1103 16-17 -0.6029513018005019 1103 18-19 -0.6539898397961537 1103 20-21 -0.6591653084756715 1103 22-23 -0.702976366009338 1103 24-25 -0.7750327865911828 1103 26-27 -0.7329020329032048 1103 28-29 -0.7821911307061882 1103 30-31 -0.7280060125454426 1103 32-33 -0.6203348126448205 1103 34-35 -0.7303799222710339 1103 36-37 -0.7178220005571134 1103 38-39 -0.7422924729120055 1103 40-41 -0.7867340736095301 1103 42-43 -0.6714133350966378 1103 44-45 -0.658775155941349 1103 46-47 -0.7741946954369467 1103 48-49 -0.8222150139669608 1103 50-51 -0.8838047933306186 1103 52-53 -0.8293071643519099 1103 54-55 -0.857959976636586 1103 56-57 -0.8979675495932398 1103 58-59 -0.8155606311167247 1103 60-61 -0.7908074744639606 1103 62-63 -0.8342638041813757 1103 64-65 -0.8826182325982472 1103 66-67 -0.9876809051545976 1103 68-69 -1.0557809986058189 1103 70-71 -1.1007936753902783 1103 72-73 -0.9060839984827211 1103 74-75 -0.9303270145009108 1103 76-77 -0.8337686553467591 1103 78-79 -0.725114188376466 1103 80-81 -0.6460243884831378 1103 82-83 -0.7600973839692173 1103 84-85 -0.765142399304807 1103 86-87 -0.7331002027069253 1103 88-89 -0.7902174133798532 1103 90-91 -0.7937996438773567 1103 92-93 -0.986602814855349 1103 94-95 -0.9410603065099252 1103 96-97 -1.0020858144705542 1103 98-99 -1.0038626997887548 1103 100 -1.1122126072585736 1104 1 -0.3532222496128625 1104 2 -0.3416255061196054 1104 3 -0.42239885699833835 1104 4 -0.2944976537493673 1104 5 -0.30597312685782185 1104 6 -0.38169927646263346 1104 7 -0.364233508598538 1104 8 -0.44208137897427946 1104 9 -0.6098074870549581 1104 10-11 -0.591239525121658 1104 12-13 -0.5944583885821402 1104 14-15 -0.7459012522001132 1104 16-17 -0.7312395131938558 1104 18-19 -0.7812227309096613 1104 20-21 -0.785569628546348 1104 22-23 -0.7269311932654929 1104 24-25 -0.8144400712152375 1104 26-27 -0.7910869520546697 1104 28-29 -0.863411110009686 1104 30-31 -0.8731573368187497 1104 32-33 -0.8578198792333822 1104 34-35 -0.8652690589059588 1104 36-37 -0.7681365597334988 1104 38-39 -0.7941561197018032 1104 40-41 -0.8234186075445038 1104 42-43 -0.7780788294960743 1104 44-45 -0.9484891770059178 1104 46-47 -1.0349954587655787 1104 48-49 -0.9224525352592892 1104 50-51 -0.900704159013948 1104 52-53 -0.8570308843093741 1104 54-55 -0.9433568889698805 1104 56-57 -0.946659712338203 1104 58-59 -0.8866025884886994 1104 60-61 -0.8926075033145864 1104 62-63 -0.9248583414930849 1104 64-65 -0.8812520966478345 1104 66-67 -0.9294429108881914 1104 68-69 -0.9793444129083824 1104 70-71 -1.027125650496437 1104 72-73 -0.9714639935630913 1104 74-75 -1.0807331722466884 1104 76-77 -1.0105457538705593 1104 78-79 -0.8315682035770493 1104 80-81 -0.919869672167593 1104 82-83 -0.9155647471325281 1104 84-85 -0.8792581742072585 1104 86-87 -0.875326267788477 1104 88-89 -0.9970065264996428 1104 90-91 -0.9104567971538096 1104 92-93 -1.047302949354421 1104 94-95 -1.1010848555329922 1104 96-97 -1.0648498047315158 1104 98-99 -1.1492731093196262 1104 100 -1.1743087770203893 1105 1 -0.39201925644511704 1105 2 -0.3794039426016056 1105 3 -0.39390371892710974 1105 4 -0.2306655479795907 1105 5 -0.29648561391933725 1105 6 -0.38710821588059474 1105 7 -0.4384634215574863 1105 8 -0.43767231225239556 1105 9 -0.4977023129587721 1105 10-11 -0.5576403531983658 1105 12-13 -0.5538903611467774 1105 14-15 -0.7533533708267868 1105 16-17 -0.7378317134986929 1105 18-19 -0.7317182674007938 1105 20-21 -0.7949556511045515 1105 22-23 -0.7649919625877715 1105 24-25 -0.7929432166089967 1105 26-27 -0.7480438487139551 1105 28-29 -0.7724444332956395 1105 30-31 -0.7270810186940793 1105 32-33 -0.7510482631204027 1105 34-35 -0.7152746525629112 1105 36-37 -0.7242357710156995 1105 38-39 -0.7687485881685419 1105 40-41 -0.858827081606357 1105 42-43 -0.8372758111247194 1105 44-45 -0.8240749948421779 1105 46-47 -0.7544333958123843 1105 48-49 -0.827152494732637 1105 50-51 -0.8448899670565382 1105 52-53 -0.8671081987618265 1105 54-55 -0.8236402230730206 1105 56-57 -0.894769977270407 1105 58-59 -0.9964459893793034 1105 60-61 -0.8870719724369209 1105 62-63 -0.9614410664662643 1105 64-65 -0.9054689125603588 1105 66-67 -0.9251815313064569 1105 68-69 -0.8735708970372826 1105 70-71 -0.96159034656241 1105 72-73 -0.9808572362656349 1105 74-75 -0.8713560328119243 1105 76-77 -0.8864560003344906 1105 78-79 -0.8637147710822006 1105 80-81 -0.7450628097253684 1105 82-83 -0.72010688172276 1105 84-85 -0.7437353785868339 1105 86-87 -0.82036307106236 1105 88-89 -0.8778480533500819 1105 90-91 -0.8905692788354109 1105 92-93 -0.9990678932199302 1105 94-95 -0.910242417432567 1105 96-97 -1.0187500259164253 1105 98-99 -1.055339596231633 1105 100 -1.0719790875401145 1106 1 -0.4298982383753085 1106 2 -0.4016447931425873 1106 3 -0.39588103805114727 1106 4 -0.26177602617510587 1106 5 -0.3050743937488747 1106 6 -0.3647214017215603 1106 7 -0.34026395800427167 1106 8 -0.4199097800153737 1106 9 -0.4258197034870719 1106 10-11 -0.5035208350165377 1106 12-13 -0.5485653827058883 1106 14-15 -0.6538878050266703 1106 16-17 -0.6669011202155417 1106 18-19 -0.6832648007242028 1106 20-21 -0.6826838615621327 1106 22-23 -0.6383810263754981 1106 24-25 -0.6756070689019467 1106 26-27 -0.7182529385474439 1106 28-29 -0.6811207099792327 1106 30-31 -0.7701445551526547 1106 32-33 -0.7350184389720127 1106 34-35 -0.7114246080216446 1106 36-37 -0.7439019798689017 1106 38-39 -0.7818560397535421 1106 40-41 -0.7966110550450409 1106 42-43 -0.7186420000959757 1106 44-45 -0.6968413753957563 1106 46-47 -0.7688767296051537 1106 48-49 -0.7752516032161338 1106 50-51 -0.8058279276398537 1106 52-53 -0.7982817787777421 1106 54-55 -0.8003383918598175 1106 56-57 -0.8075768413823425 1106 58-59 -0.8812104065392532 1106 60-61 -0.8446069274707781 1106 62-63 -0.9413623274292959 1106 64-65 -0.9737701786219475 1106 66-67 -0.9441561917784114 1106 68-69 -0.9561546421384577 1106 70-71 -0.8448916715786723 1106 72-73 -0.9292922846918046 1106 74-75 -0.9969591491961509 1106 76-77 -0.8448800526992386 1106 78-79 -0.6878984929267773 1106 80-81 -0.6309566661207526 1106 82-83 -0.7555587102728367 1106 84-85 -0.7712711203605238 1106 86-87 -0.8011230129533935 1106 88-89 -0.8316414200964815 1106 90-91 -0.8400567188490378 1106 92-93 -0.7961696209168743 1106 94-95 -0.7708721670947973 1106 96-97 -0.7239523583255938 1106 98-99 -0.8086013647008095 1106 100 -0.7274187531329943 1107 1 -0.48976939025463295 1107 2 -0.4667951536327095 1107 3 -0.47773856299153294 1107 4 -0.3324420650928772 1107 5 -0.32687403811316784 1107 6 -0.40442060094272136 1107 7 -0.4366650416691016 1107 8 -0.41243863717989626 1107 9 -0.5749936550998669 1107 10-11 -0.5957907457618177 1107 12-13 -0.6581449704929838 1107 14-15 -0.8326087467032153 1107 16-17 -0.7679018610124331 1107 18-19 -0.8113659401150528 1107 20-21 -0.7975145289576275 1107 22-23 -0.802643760567733 1107 24-25 -0.8410232916889626 1107 26-27 -0.9091041966386868 1107 28-29 -0.8774851860416533 1107 30-31 -0.8687954935175597 1107 32-33 -0.8463878093478456 1107 34-35 -0.9167258942418997 1107 36-37 -0.8669665316069839 1107 38-39 -0.8969816626812772 1107 40-41 -0.9496583636381573 1107 42-43 -0.8790734378138367 1107 44-45 -0.9807784182994652 1107 46-47 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2311 28-29 0.6657067884124928 2311 30-31 0.8779137928384202 2311 32-33 0.9616013901974938 2311 34-35 1.0187380471803849 2311 36-37 1.0547698863196615 2311 38-39 1.0675067777511416 2311 40-41 1.0776677743239063 2311 42-43 1.0311125364165505 2311 44-45 1.0064301006373455 2311 46-47 0.6996256545580053 2311 48-49 0.9802496038411803 2311 50-51 1.1274037870950977 2311 52-53 1.0601110365857451 2311 54-55 1.1165692851577091 2311 56-57 1.1448013948208882 2311 58-59 1.1895655631375064 2311 60-61 1.2607059930537545 2311 62-63 1.2221865630496254 2311 64-65 1.2282466805970245 2311 66-67 1.2643220631134895 2311 68-69 1.3078416981881062 2311 70-71 1.2956809141787105 2311 72-73 1.2710674271492195 2311 74-75 1.2768686810103205 2311 76-77 1.2513557788714422 2311 78-79 0.9933954740455491 2311 80-81 0.9487332757649583 2311 82-83 1.0373081261909434 2311 84-85 1.0875684930433565 2311 86-87 1.086507223809921 2311 88-89 1.1212711133337159 2311 90-91 1.1816131939233152 2311 92-93 1.218912301755143 2311 94-95 1.283941920323059 2311 96-97 1.3492444551655396 2311 98-99 1.3424689067713693 2311 100 1.3990014543908238 2312 1 0.2753578874370035 2312 2 0.39428617338367644 2312 3 0.44481493807285943 2312 4 0.23178669786420159 2312 5 0.3564501938837097 2312 6 0.4202945107882954 2312 7 0.3361097724066866 2312 8 0.42953382833582054 2312 9 0.6134868819689601 2312 10-11 0.6560934255433324 2312 12-13 0.686030373156008 2312 14-15 0.8947889969266711 2312 16-17 0.8715593364085237 2312 18-19 0.8716625546813503 2312 20-21 0.8902158486215299 2312 22-23 0.9691040645848688 2312 24-25 0.9408830524443346 2312 26-27 1.0319589960513156 2312 28-29 1.0034127135740718 2312 30-31 0.9959897313797512 2312 32-33 0.8356174339563154 2312 34-35 0.9546151997975798 2312 36-37 0.9799001516358814 2312 38-39 0.8646767080999069 2312 40-41 0.9660786214074477 2312 42-43 1.1006353488343947 2312 44-45 0.8410388997097016 2312 46-47 0.868872936054288 2312 48-49 0.9957463475198338 2312 50-51 0.8338933340338315 2312 52-53 0.995975901819719 2312 54-55 0.9161110049023264 2312 56-57 0.9356899077572791 2312 58-59 1.0299492984241425 2312 60-61 1.0921787233747793 2312 62-63 1.113821885216261 2312 64-65 1.0467508401079826 2312 66-67 1.0597668675186327 2312 68-69 1.132898642948092 2312 70-71 1.0252775547154513 2312 72-73 1.0254119852731876 2312 74-75 1.0771680111409196 2312 76-77 1.0631232595176812 2312 78-79 1.0039408297226444 2312 80-81 0.926411354193732 2312 82-83 0.9642113074407987 2312 84-85 0.9856709647107422 2312 86-87 1.0222844088316059 2312 88-89 1.020025918364869 2312 90-91 1.0976356964992924 2312 92-93 1.1557527831560712 2312 94-95 1.172134761625621 2312 96-97 0.7696685342673675 2312 98-99 0.5981561061257317 2312 100 0.7631958054862906 2313 1 0.06191610972637562 2313 2 0.2416215981854677 2313 3 0.2802883582164952 2313 4 0.16814862976343647 2313 5 0.33287977570797267 2313 6 0.23342866188849598 2313 7 0.26130202019388804 2313 8 0.39419503202698536 2313 9 0.5799951069004976 2313 10-11 0.3442035352561632 2313 12-13 0.4229307230335735 2313 14-15 0.4856680000358651 2313 16-17 0.7608488963527549 2313 18-19 0.6799803252497156 2313 20-21 0.6605434367244811 2313 22-23 0.6233485378031531 2313 24-25 0.5472802652778981 2313 26-27 0.7797447882420059 2313 28-29 0.5854523610749638 2313 30-31 0.7981828593356326 2313 32-33 0.8873736811068582 2313 34-35 0.945739811862417 2313 36-37 0.9581658448300132 2313 38-39 0.945872209329508 2313 40-41 0.9449747166735492 2313 42-43 0.9708587992479636 2313 44-45 0.8570560971567787 2313 46-47 0.8874254783080247 2313 48-49 0.9877419540554726 2313 50-51 0.8945549117550371 2313 52-53 0.9067385565157338 2313 54-55 0.8361525838073547 2313 56-57 0.5935835541343337 2313 58-59 0.7195756421036279 2313 60-61 0.5909381917538497 2313 62-63 0.936770426887918 2313 64-65 0.9865202716981756 2313 66-67 1.0010955080472073 2313 68-69 1.029168873616868 2313 70-71 0.9282324316497252 2313 72-73 0.9456152682952208 2313 74-75 1.0688641913215164 2313 76-77 0.9725647189774733 2313 78-79 0.8129206211513136 2313 80-81 0.7597260878508649 2313 82-83 0.8848742233072144 2313 84-85 0.8773716060365686 2313 86-87 0.882904455651218 2313 88-89 0.9262469427406153 2313 90-91 0.8073555322148884 2313 92-93 0.9650805300274037 2313 94-95 1.1008990993774432 2313 96-97 1.1598400183890192 2313 98-99 1.140777161652128 2313 100 1.2167556010186544 2314 1 -0.32681180633333895 2314 2 0.07325470167711501 2314 3 0.3005328393856175 2314 4 0.24795973448502195 2314 5 0.33344871098042006 2314 6 0.3951398395958421 2314 7 0.47120633143375557 2314 8 0.45212151654970967 2314 9 0.454535997979967 2314 10-11 0.5795039998979732 2314 12-13 0.42495669042762074 2314 14-15 0.715732211792556 2314 16-17 0.8290884003397565 2314 18-19 0.8288259845313348 2314 20-21 0.8420512867765169 2314 22-23 0.9252838172020006 2314 24-25 0.7890557983552071 2314 26-27 0.7146719944591595 2314 28-29 0.7729482416419415 2314 30-31 0.8265281110579323 2314 32-33 0.8324019260560505 2314 34-35 0.9133658302316121 2314 36-37 0.8380466171004954 2314 38-39 0.6766458328039562 2314 40-41 0.6651715635914854 2314 42-43 0.8385561404978645 2314 44-45 0.8718532517396795 2314 46-47 0.8575908058944108 2314 48-49 0.8723717901186703 2314 50-51 0.8426750368406815 2314 52-53 0.847145024373269 2314 54-55 0.7850627330018867 2314 56-57 0.778788689805026 2314 58-59 0.8540465965482866 2314 60-61 0.9764012492832066 2314 62-63 0.9014365759421565 2314 64-65 0.8788783530733824 2314 66-67 0.6616280743950185 2314 68-69 0.557896476750841 2314 70-71 0.7537861212407009 2314 72-73 0.9001602727608429 2314 74-75 0.9794936966206933 2314 76-77 0.8154476539125071 2314 78-79 0.7225371994429111 2314 80-81 0.7834233719578023 2314 82-83 0.7579324964288006 2314 84-85 0.7728219832708163 2314 86-87 0.828855944905623 2314 88-89 0.7824713984765843 2314 90-91 0.917885286766218 2314 92-93 0.8561335825801741 2314 94-95 0.8741738203595482 2314 96-97 0.8954265757644819 2314 98-99 0.970848281962553 2314 100 1.0055973192500396 2315 1 0.3701610764972898 2315 2 0.44219646441396776 2315 3 0.46768657887228215 2315 4 0.334447221192292 2315 5 0.37247858337021 2315 6 0.41889002086034566 2315 7 0.40314249309751915 2315 8 0.2635811338399279 2315 9 0.4960334089186773 2315 10-11 0.5436904772640787 2315 12-13 0.3619824571764525 2315 14-15 0.6265495862971022 2315 16-17 0.7648309654741325 2315 18-19 0.7961714489914016 2315 20-21 0.5402101303694238 2315 22-23 0.8465538846928169 2315 24-25 0.8971088514536163 2315 26-27 0.789994418690604 2315 28-29 0.8006550493323914 2315 30-31 0.8576360072417089 2315 32-33 0.8005110427541382 2315 34-35 0.8595241557101545 2315 36-37 0.8639902498827823 2315 38-39 0.9355668407554276 2315 40-41 0.8973932168445842 2315 42-43 0.9989451643280987 2315 44-45 0.994221706580646 2315 46-47 0.9935875189647874 2315 48-49 0.8895075164455264 2315 50-51 0.9481532277001108 2315 52-53 1.0299149272154935 2315 54-55 0.8456951799518038 2315 56-57 0.8535672450061114 2315 58-59 0.878793468225112 2315 60-61 0.9136448733043849 2315 62-63 1.007393380611532 2315 64-65 1.0908419234402587 2315 66-67 1.0680008433311272 2315 68-69 1.0967097380617474 2315 70-71 0.9959084037837229 2315 72-73 1.1389349776018065 2315 74-75 0.5351591941313316 2315 76-77 0.49756319190554876 2315 78-79 0.7696838318505925 2315 80-81 0.8096606909954076 2315 82-83 0.7911096779470697 2315 84-85 0.8874200065814293 2315 86-87 0.882447021254233 2315 88-89 0.8982878591059311 2315 90-91 0.9481641244378203 2315 92-93 0.9797816515090254 2315 94-95 1.0385926939215153 2315 96-97 1.1019675808157245 2315 98-99 1.1359868133575652 2315 100 1.073262149103094 2316 1 0.28745245799904495 2316 2 0.04970301047624304 2316 3 0.20544685550260766 2316 4 0.017244368117395936 2316 5 0.2741932392169062 2316 6 0.33935727374458224 2316 7 0.4204999844814665 2316 8 0.44413081211280314 2316 9 0.6332688548147445 2316 10-11 0.5856365482518129 2316 12-13 0.3316426042604377 2316 14-15 0.458774792990738 2316 16-17 0.7336550956193264 2316 18-19 0.7522917689769884 2316 20-21 0.7613800155803645 2316 22-23 0.7652813324770875 2316 24-25 0.44913083614026306 2316 26-27 0.6625197092532673 2316 28-29 0.9409629434099216 2316 30-31 0.5325981783345455 2316 32-33 0.6976480952998472 2316 34-35 0.8128862881529599 2316 36-37 0.84711569290684 2316 38-39 0.7732346934025713 2316 40-41 0.8127266993802706 2316 42-43 0.7939373884921608 2316 44-45 0.6232334612308676 2316 46-47 0.7495882033605596 2316 48-49 0.8164191461324108 2316 50-51 0.8630801974767053 2316 52-53 0.8698630170179982 2316 54-55 0.7768684527690723 2316 56-57 0.8926286756377095 2316 58-59 0.7576627295359657 2316 60-61 0.8576562002072379 2316 62-63 0.8824255380470021 2316 64-65 0.8994429489645555 2316 66-67 0.923435621788542 2316 68-69 0.8815291370156046 2316 70-71 0.8827933450906684 2316 72-73 0.9180881944147217 2316 74-75 0.959905410094855 2316 76-77 0.7502101842305322 2316 78-79 0.450642791782883 2316 80-81 0.4728747808406837 2316 82-83 0.39504967033927585 2316 84-85 0.5481334548056651 2316 86-87 0.6302247591428731 2316 88-89 0.5649740162847614 2316 90-91 0.3996570582041947 2316 92-93 0.3288349549407812 2316 94-95 0.40264516859276256 2316 96-97 0.5877151533494498 2316 98-99 0.7604512507475718 2316 100 0.42336358633422577 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 2.0 11 6.0 12 30.0 13 178.0 14 681.0 15 1938.0 16 4688.0 17 9504.0 18 16025.0 19 23579.0 20 33651.0 21 46696.0 22 57934.0 23 60857.0 24 62770.0 25 68088.0 26 81413.0 27 100142.0 28 125118.0 29 153970.0 30 191095.0 31 240449.0 32 305508.0 33 401978.0 34 575366.0 35 807476.0 36 1267669.0 37 1924303.0 38 1648523.0 39 252339.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66334952024491 19.948904469162386 13.431842004645391 23.95590400594731 2 15.600979011938193 20.0192129497503 40.51678793231057 23.86302010600093 3 19.144514400317373 25.145459094131517 29.34778665235327 26.362239853197845 4 12.69009284620669 16.23731044505343 38.09908867978976 32.973508028950114 5 13.387211842683545 37.56921087577276 34.52474184297544 14.518835438568253 6 31.4318445483521 36.84030526585851 17.624424766671616 14.103425419117771 7 28.039264393548695 31.838356703139098 21.885457147057473 18.236921756254727 8 26.356000194280288 35.85134296249788 19.485403038832068 18.307253804389763 9 26.26802029952903 15.880496622004536 20.638894571770543 37.212588506695894 10-11 25.771266076204775 26.132399775324 27.355987020978468 20.740347127492758 12-13 25.740254919625684 23.508022718741273 27.995283148470623 22.75643921316242 14-15 22.917943823967164 25.084414250336756 25.679380717737832 26.318261207958248 16-17 22.25379286432583 27.87284127683896 26.67211693061508 23.20124892822013 18-19 21.858015615928196 27.407932237304156 28.756573061147122 21.97747908562053 20-21 22.916702734772944 26.632331208205436 28.512253101379514 21.93871295564211 22-23 22.88609396288183 26.44480306726506 28.28629422284398 22.38280874700913 24-25 22.45776935550402 26.34288879039574 28.844326549419115 22.35501530468112 26-27 22.474777272385367 26.647344117554777 28.24836180122067 22.62951680883918 28-29 22.107875583388257 26.982887829327122 28.49585954318778 22.413377044096844 30-31 22.809882019094534 26.679186077938798 28.427137132756613 22.083794770210048 32-33 22.804887043064497 26.755158995907884 28.03264237840603 22.40731158262159 34-35 22.803655705680608 26.764807331459206 28.416975560305364 22.014561402554826 36-37 22.64415917682535 26.574025158206656 28.230039505413362 22.55177615955463 38-39 22.671067332972225 26.738379915325883 28.43478772222375 22.155765029478143 40-41 22.61545778505905 26.19913053718545 28.71306610348289 22.47234557427261 42-43 22.524233351591437 26.328135386513463 28.290355879435054 22.857275382460045 44-45 22.475490553829776 26.877910128317627 28.25363374356295 22.392965574289647 46-47 22.475133687548333 26.682979743791513 27.813392660060863 23.028493908599295 48-49 22.87351929509852 26.65163938567347 27.88259457605642 22.592246743171586 50-51 22.712184917401157 26.738653185322196 27.77381715680336 22.775344740473287 52-53 22.46964986274805 27.015918295083303 27.539858377451015 22.974573464717633 54-55 22.374742285190578 26.915427704203882 27.66922634766179 23.040603662943745 56-57 22.902267304745525 26.705633981994843 27.389842794760344 23.002255918499294 58-59 22.366673304759985 26.99591609528835 27.745650325335514 22.89176027461615 60-61 22.530923543374506 27.30611072735105 27.400543278188096 22.76242245108635 62-63 22.676750776879903 26.942639965865794 27.21815749630978 23.16245176094452 64-65 22.26162201192976 26.92545217745853 27.398261475659158 23.414664334952555 66-67 22.40392715844517 26.782681155106005 27.378561195596003 23.43483049085283 68-69 22.202079739282617 26.814894240829773 27.517125364056067 23.465900655831547 70-71 22.235828623429015 26.524109233796906 27.59670997297644 23.64335216979764 72-73 22.483170594547662 26.72931721238353 27.52982569017602 23.25768650289279 74-75 22.583654164316613 26.934859971417502 27.247845288989048 23.233640575276837 76-77 22.47854222799099 26.819432586260344 27.113816993568356 23.588208192180307 78-79 22.338181817966923 26.807771908258527 27.2757207735694 23.578325500205153 80-81 22.145893861961344 27.091198731254963 27.380170304440902 23.382737102342794 82-83 22.653506035486156 26.597888686685707 27.870592433515846 22.87801284431229 84-85 22.844554903514666 26.830960082505356 27.163884219372264 23.16060079460771 86-87 22.091474340938507 27.209942914041402 27.419735600320134 23.27884714469996 88-89 22.4450021366287 26.586372158205485 27.397321347005 23.57130435816082 90-91 22.443322404110567 26.53545254200346 27.457566178766125 23.56365887511985 92-93 22.56875303348937 26.706877339713987 27.332375384655762 23.391994242140886 94-95 21.965629553112095 26.665541885159417 27.650330446426963 23.71849811530153 96-97 22.052048007442487 26.617945497399575 27.46649368305709 23.863512812100847 98-99 21.80558767541457 26.88027651795367 27.330769483995134 23.983366322636627 100 22.04870902834332 26.593102326457284 27.085266393125284 24.27292225207411 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1132.0 1 817.5 2 1008.5 3 1502.5 4 1090.5 5 805.5 6 1250.5 7 2397.5 8 3671.5 9 4324.5 10 4000.5 11 3566.0 12 3890.5 13 4464.5 14 5184.5 15 6070.0 16 7084.0 17 8154.0 18 9096.5 19 10030.5 20 11173.0 21 12576.0 22 14471.0 23 17085.0 24 20304.5 25 24412.5 26 30358.0 27 37777.5 28 47138.0 29 60447.0 30 77249.0 31 94900.5 32 117642.0 33 141459.0 34 161135.5 35 180931.5 36 200440.0 37 227985.0 38 245700.0 39 246600.0 40 245841.0 41 244432.5 42 250056.5 43 263147.5 44 280619.5 45 308850.0 46 334275.5 47 352132.5 48 367912.0 49 370147.0 50 363137.5 51 364071.5 52 358865.0 53 341293.0 54 323920.0 55 291095.0 56 256368.0 57 219470.0 58 184108.5 59 165017.5 60 135612.0 61 101422.0 62 78342.5 63 62030.5 64 45345.0 65 28829.5 66 19448.5 67 14354.5 68 11015.5 69 8269.0 70 6261.5 71 4779.5 72 3669.0 73 2941.0 74 2229.0 75 1496.5 76 971.5 77 562.5 78 318.5 79 212.0 80 137.5 81 77.0 82 47.5 83 34.5 84 23.0 85 10.0 86 8.5 87 10.0 88 6.5 89 4.5 90 4.0 91 3.5 92 2.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044043956033567 2 0.0 3 0.0 4 0.037508858720295586 5 0.0 6 0.0 7 0.001004490545439548 8 0.0 9 0.010848497890747119 10-11 4.4906636149062137E-4 12-13 5.908767914350282E-6 14-15 1.3590166203005647E-4 16-17 3.013471636318644E-4 18-19 0.0 20-21 5.908767914350282E-6 22-23 0.0 24-25 5.613329518632768E-4 26-27 4.667926652336723E-4 28-29 1.4181042994440676E-4 30-31 0.0024580474523697173 32-33 0.015870950617944857 34-35 0.026459462720460562 36-37 0.031363740089371296 38-39 0.022671942487362033 40-41 0.0012940201732427117 42-43 7.090521497220338E-5 44-45 0.010948946945291072 46-47 0.0178267527975948 48-49 0.0033030012641218076 50-51 0.010943038177376723 52-53 0.010860315426575819 54-55 0.010109901901453331 56-57 4.4315759357627116E-4 58-59 0.008697706369923614 60-61 0.004797919546452429 62-63 0.0012703851015853107 64-65 0.035310797056157285 66-67 0.06023988888680113 68-69 0.05941266137879208 70-71 0.05176671569762282 72-73 0.0450779904185783 74-75 0.03519262169787028 76-77 0.03135783132145695 78-79 0.014742375946303954 80-81 0.001134483439555254 82-83 0.03825927224541807 84-85 0.05162490526767841 86-87 0.057226417250482484 88-89 0.06998935594547909 90-91 0.04839871798644316 92-93 0.03901559453845491 94-95 0.012981563107827568 96-97 0.0036988887143832765 98-99 0.02287284059644994 100 0.033443626395222595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8462001.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.66036290352228 #Duplication Level Percentage of deduplicated Percentage of total 1 77.47568240710591 50.870814230486104 2 13.989565286392109 18.37119867134047 3 4.28431565709068 8.439291625134498 4 1.6952851135821452 4.452521431309706 5 0.845017383314998 2.7742074024123777 6 0.4641188287850799 1.8284526437031643 7 0.29157725600175644 1.3401547910441984 8 0.19097897230420038 1.0031798902748423 9 0.13497814035055858 0.7976442311514216 >10 0.592768667609191 6.510923840088445 >50 0.024519485137121804 1.0970675504702738 >100 0.010404888916204712 1.2464463603777143 >500 4.052443000020666E-4 0.16802830078556152 >1k 3.151392688319116E-4 0.3616545898702628 >5k 0.0 0.0 >10k+ 6.752984114513644E-5 0.7384144415509573 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21151 0.24995270031284564 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17319 0.2046679030172651 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11490 0.13578348667176948 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.1817535828700563E-5 0.0 5 0.0 0.0 0.0 1.1817535828700563E-5 0.0 6 1.1817535828700563E-5 0.0 0.0 1.1817535828700563E-5 0.0 7 1.1817535828700563E-5 0.0 0.0 1.1817535828700563E-5 0.0 8 1.1817535828700563E-5 0.0 0.0 1.1817535828700563E-5 0.0 9 1.1817535828700563E-5 0.0 0.0 1.1817535828700563E-5 1.1817535828700563E-5 10-11 1.1817535828700563E-5 0.0 0.0 1.1817535828700563E-5 1.1817535828700563E-5 12-13 1.1817535828700563E-5 0.0 0.0 3.545260748610169E-5 1.1817535828700563E-5 14-15 1.1817535828700563E-5 0.0 0.0 6.49964470578531E-5 2.3635071657401126E-5 16-17 1.1817535828700563E-5 0.0 0.0 1.0635782245830507E-4 3.545260748610169E-5 18-19 1.1817535828700563E-5 0.0 0.0 1.359016620300565E-4 5.3178911229152536E-5 20-21 1.1817535828700563E-5 0.0 0.0 1.8908057325920904E-4 5.908767914350282E-5 22-23 1.1817535828700563E-5 0.0 0.0 4.254312898332203E-4 8.863151871525423E-5 24-25 1.1817535828700563E-5 0.0 0.0 8.804064192381919E-4 1.2408412620135592E-4 26-27 1.1817535828700563E-5 0.0 0.0 0.0016071848727032766 1.5362796577310733E-4 28-29 1.1817535828700563E-5 0.0 0.0 0.004337035649133107 1.5362796577310733E-4 30-31 1.1817535828700563E-5 0.0 0.0 0.015870950617944857 1.654455016018079E-4 32-33 1.1817535828700563E-5 0.0 0.0 0.043565345832504626 1.89080573259209E-4 34-35 1.1817535828700563E-5 0.0 0.0 0.08311863825116542 2.1271564491661014E-4 36-37 1.1817535828700563E-5 0.0 0.0 0.1474178506951252 2.3635071657401128E-4 38-39 1.1817535828700563E-5 0.0 0.0 0.24258446672365083 2.3635071657401128E-4 40-41 1.1817535828700563E-5 0.0 0.0 0.34602335783226684 2.599857882314124E-4 42-43 1.1817535828700563E-5 0.0 0.0 0.4474769029216612 2.658945561457627E-4 44-45 1.1817535828700563E-5 0.0 0.0 0.5656522612086669 2.836208598888135E-4 46-47 1.1817535828700563E-5 0.0 0.0 0.7049101034140743 3.0725593154621466E-4 48-49 1.1817535828700563E-5 0.0 0.0 0.8563281899872146 3.4270853903231634E-4 50-51 1.1817535828700563E-5 0.0 0.0 1.0105883939271574 3.4270853903231634E-4 52-53 1.1817535828700563E-5 0.0 0.0 1.1630996025644524 3.8406991443276834E-4 54-55 1.1817535828700563E-5 0.0 0.0 1.3292600650838968 4.254312898332203E-4 56-57 1.1817535828700563E-5 0.0 0.0 1.5189196975987123 4.313400577475706E-4 58-59 1.1817535828700563E-5 0.0 0.0 1.7330948081901667 4.4315759357627116E-4 60-61 1.1817535828700563E-5 0.0 0.0 1.9508860847452039 4.6088389731932197E-4 62-63 1.1817535828700563E-5 0.0 0.0 2.174196150532244 4.963365048054237E-4 64-65 1.1817535828700563E-5 0.0 0.0 2.40404722240047 4.963365048054237E-4 66-67 1.1817535828700563E-5 0.0 0.0 2.653509494976425 5.140628085484745E-4 68-69 1.1817535828700563E-5 0.0 0.0 2.9280663048846245 5.376978802058756E-4 70-71 1.1817535828700563E-5 0.0 0.0 3.2213420915454867 5.67241719777627E-4 72-73 1.1817535828700563E-5 0.0 0.0 3.5141806293806868 6.263293989211299E-4 74-75 1.1817535828700563E-5 0.0 0.0 3.8028003069250405 6.263293989211299E-4 76-77 1.1817535828700563E-5 0.0 0.0 4.125034965134134 6.322381668354801E-4 78-79 1.1817535828700563E-5 0.0 0.0 4.472535514944988 6.499644705785311E-4 80-81 1.1817535828700563E-5 0.0 0.0 4.839322283228281 6.499644705785311E-4 82-83 1.1817535828700563E-5 0.0 0.0 5.209530228133984 6.854170780646327E-4 84-85 1.1817535828700563E-5 0.0 0.0 5.576541529598023 6.854170780646327E-4 86-87 1.1817535828700563E-5 0.0 0.0 5.960020567239356 6.854170780646327E-4 88 1.1817535828700563E-5 0.0 0.0 6.268706420620844 6.972346138933332E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13140 0.0 45.271908 1 GTATCAA 22040 0.0 37.914867 1 GTACTAG 5375 0.0 32.63353 1 ATCAACG 25900 0.0 30.982458 3 CAACGCA 26030 0.0 30.687883 5 TATCAAC 28510 0.0 29.132504 2 TACCTGG 6025 0.0 28.709742 2 AACGCAG 29050 0.0 28.112263 6 TCAACGC 29130 0.0 27.772982 4 GTACATG 29965 0.0 26.961407 1 GTGGTAT 5360 0.0 26.583458 1 CTGTGCG 7130 0.0 26.294834 9 TACATGG 30240 0.0 26.253448 2 ACATGGG 30030 0.0 25.296951 3 ACGCAGA 31750 0.0 25.277628 7 GTACCTG 7230 0.0 25.236383 1 TAGTACT 7805 0.0 24.87478 4 CTAGTAC 7990 0.0 24.710678 3 CGCAGAG 32480 0.0 24.680279 8 GCATCGT 6075 0.0 24.364153 8 >>END_MODULE