FastQCFastQC Report
Mon 6 Jun 2016
ERR1211258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5844745
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205190.35106749738440257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157050.2687029117609066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100320.17164136331011876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94300.050.1181
GTATCAA170550.041.373441
TACCTGG47750.039.826862
ATCAACG201050.033.9034733
CAACGCA201250.033.799125
TATCAAC215250.032.3876842
ACCTGGG58000.031.9336433
GTACCTG61000.031.5620521
AACGCAG220600.031.303766
TCAACGC221700.030.9780164
GTACTAG32250.030.5062141
GTACATG223650.029.5718961
TACATGG226850.028.9054932
GTGGTAT40300.028.2671531
ACGCAGA244500.027.8972457
ACATGGG228800.027.6752663
CGCAGAG250050.027.2592538
GCATCGT32400.026.1156168
CATGGGG179150.026.0031474
CCTGGGG56800.024.8280144
Produced by FastQC (version 0.11.5)