FastQCFastQC Report
Mon 6 Jun 2016
ERR1211258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5844745
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185520.3174133345423966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152330.2606272814297288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100650.1722059730578494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94900.049.7415241
TACCTGG48200.041.438132
GTATCAA169050.039.771541
ACCTGGG59500.032.8591963
CAACGCA202800.032.178535
ATCAACG203300.032.017913
GTACCTG64250.031.75911
TATCAAC218200.030.5407562
AACGCAG223300.029.7714276
TCAACGC223100.029.281674
GTACTAG30800.028.851121
GTACATG216750.026.7674881
TACATGG216100.026.4663522
ACGCAGA249450.026.1984487
GTGGTAT38150.025.8807181
CTAACGC14200.025.8158763
CCTGGGG56650.025.801274
CGCAGAG255850.025.5612458
ACATGGG216550.025.3492573
TCTAACG14700.024.9365022
Produced by FastQC (version 0.11.5)