##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211258_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5844745 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.169938808279916 33.0 31.0 34.0 30.0 34.0 2 32.308475391141954 34.0 31.0 34.0 31.0 34.0 3 32.385764306227216 34.0 31.0 34.0 30.0 34.0 4 35.99524530154866 37.0 35.0 37.0 35.0 37.0 5 35.88615910531597 37.0 35.0 37.0 35.0 37.0 6 35.915279622977565 37.0 35.0 37.0 35.0 37.0 7 35.85363074009217 37.0 35.0 37.0 35.0 37.0 8 35.840722734695866 37.0 35.0 37.0 35.0 37.0 9 37.44783869954976 39.0 37.0 39.0 34.0 39.0 10-11 37.426407653370674 39.0 37.0 39.0 34.0 39.0 12-13 37.327575625626096 39.0 37.0 39.0 34.0 39.0 14-15 38.562170291432736 40.0 38.0 41.0 34.0 41.0 16-17 38.513161652048126 40.0 38.0 41.0 34.0 41.0 18-19 38.53642588342177 40.0 38.0 41.0 34.0 41.0 20-21 38.49568372957246 40.0 38.0 41.0 34.0 41.0 22-23 38.36980056443865 40.0 38.0 41.0 34.0 41.0 24-25 38.30997117923879 40.0 38.0 41.0 34.0 41.0 26-27 38.17948687239563 40.0 38.0 41.0 34.0 41.0 28-29 38.15050904701574 40.0 38.0 41.0 33.5 41.0 30-31 38.15677356326067 40.0 37.5 41.0 34.0 41.0 32-33 38.01824698938961 40.0 37.0 41.0 33.0 41.0 34-35 37.87621093820175 40.0 37.0 41.0 33.0 41.0 36-37 37.730378399741994 40.0 37.0 41.0 33.0 41.0 38-39 37.64643290682484 40.0 37.0 41.0 33.0 41.0 40-41 37.54900872493154 40.0 36.5 41.0 32.5 41.0 42-43 37.40858591777742 40.0 36.0 41.0 32.0 41.0 44-45 37.27592367160586 40.0 36.0 41.0 31.5 41.0 46-47 37.0487914357256 39.5 36.0 41.0 31.0 41.0 48-49 36.824283523062164 39.0 35.5 41.0 31.0 41.0 50-51 36.6298101114762 39.0 35.0 41.0 30.0 41.0 52-53 36.43454983921454 39.0 35.0 40.5 30.0 41.0 54-55 36.203452075325785 38.5 35.0 40.0 29.5 41.0 56-57 35.95955418072131 38.0 35.0 40.0 29.0 41.0 58-59 35.69624654283463 38.0 34.5 40.0 28.5 41.0 60-61 35.432888175617585 37.5 34.0 40.0 28.0 41.0 62-63 35.12819652525474 37.0 34.0 40.0 28.0 41.0 64-65 34.7561724249732 36.5 34.0 39.5 27.0 41.0 66-67 34.448172503676375 36.0 33.5 39.0 26.5 40.5 68-69 34.116478306581385 35.5 33.0 39.0 26.0 40.0 70-71 33.78600743060647 35.0 33.0 38.0 26.0 40.0 72-73 33.439680088695056 35.0 33.0 37.5 26.0 40.0 74-75 33.0967294552628 35.0 32.0 37.0 26.0 39.0 76-77 32.10102194364339 34.0 31.0 36.0 24.5 38.5 78-79 32.58933845702421 35.0 32.0 36.0 25.5 38.5 80-81 32.5169192154662 35.0 32.0 36.0 25.5 37.5 82-83 32.260878447220534 35.0 32.0 35.0 25.0 37.0 84-85 32.00264382791721 34.0 32.0 35.0 25.0 36.5 86-87 31.766483225529942 34.0 31.0 35.0 24.5 36.0 88-89 31.55705877673021 34.0 31.0 35.0 24.0 36.0 90-91 31.344079681833854 34.0 31.0 35.0 24.0 35.5 92-93 31.16124578574429 34.0 31.0 35.0 23.0 35.0 94-95 30.977176677511167 34.0 31.0 35.0 23.0 35.0 96-97 30.802140298678555 34.0 30.0 35.0 20.5 35.0 98-99 30.631323778881715 34.0 30.0 35.0 20.0 35.0 100 28.868749278197765 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.36951630259476786 1101 2 -0.35257837111914014 1101 3 -0.39871811669993207 1101 4 -0.24677107135954657 1101 5 -0.2288261256245363 1101 6 -0.36112294845279536 1101 7 -0.31560829555715486 1101 8 -0.3819308356929909 1101 9 -0.512675857860927 1101 10-11 -0.49267158965761837 1101 12-13 -0.5280412372894361 1101 14-15 -0.7637384081560299 1101 16-17 -0.6492787859732729 1101 18-19 -0.6354935519757205 1101 20-21 -0.6452603104609125 1101 22-23 -0.757100633886509 1101 24-25 -0.7081755742359164 1101 26-27 -0.7653983216528175 1101 28-29 -0.8396397321529179 1101 30-31 -0.6036856887685644 1101 32-33 -0.7059502619758504 1101 34-35 -0.695766712397905 1101 36-37 -0.552020242089938 1101 38-39 -0.6242974885399803 1101 40-41 -0.7737574003472218 1101 42-43 -0.7558200786653302 1101 44-45 -0.7230463473888875 1101 46-47 -0.8058833680686774 1101 48-49 -0.7303594297073204 1101 50-51 -0.8255277349734129 1101 52-53 -0.8933299217007757 1101 54-55 -0.7857945214485298 1101 56-57 -0.887319209512043 1101 58-59 -0.8331683298014312 1101 60-61 -0.911129958667594 1101 62-63 -0.9003463803728025 1101 64-65 -0.8983789742909849 1101 66-67 -0.9278092831618565 1101 68-69 -0.9347484106087549 1101 70-71 -0.9464146183514472 1101 72-73 -0.927662687401309 1101 74-75 -1.0112308983009761 1101 76-77 -0.9872447040404033 1101 78-79 -0.7700743225673286 1101 80-81 -0.7845932691232633 1101 82-83 -0.8091298479683928 1101 84-85 -0.6517346602249106 1101 86-87 -0.9441062266036795 1101 88-89 -0.9286879508006649 1101 90-91 -0.9600388629329188 1101 92-93 -0.9326306174938992 1101 94-95 -0.9415461683077346 1101 96-97 -1.0246503633661668 1101 98-99 -1.083059279421004 1101 100 -0.9587260842207108 1102 1 -0.33889256208885854 1102 2 -0.3223296960274169 1102 3 -0.3236195978794427 1102 4 -0.17451911760773697 1102 5 -0.27452216145271535 1102 6 -0.2852554334978876 1102 7 -0.3146148673334963 1102 8 -0.328139559704411 1102 9 -0.4722483020817805 1102 10-11 -0.48464208894493055 1102 12-13 -0.4827797901686708 1102 14-15 -0.554130871574678 1102 16-17 -0.627531967054189 1102 18-19 -0.6377752420758398 1102 20-21 -0.7247795270519646 1102 22-23 -0.6266771475201267 1102 24-25 -0.6285822336166689 1102 26-27 -0.6614796144973027 1102 28-29 -0.8206111436247596 1102 30-31 -0.7188652850365642 1102 32-33 -0.6565216783627932 1102 34-35 -0.6567571626498747 1102 36-37 -0.7649765873380474 1102 38-39 -0.7530578644583059 1102 40-41 -0.6525125359027584 1102 42-43 -0.6809205852096412 1102 44-45 -0.7945224744644293 1102 46-47 -0.8155902547004814 1102 48-49 -0.7564343184670648 1102 50-51 -0.7709202995852777 1102 52-53 -0.870354870164519 1102 54-55 -0.8085325640248513 1102 56-57 -0.8151988069365288 1102 58-59 -0.8532323778768784 1102 60-61 -0.7525548458371674 1102 62-63 -0.7901766432149415 1102 64-65 -0.7380261135588668 1102 66-67 -0.7541766203058344 1102 68-69 -0.9034885623407547 1102 70-71 -0.9783884208743387 1102 72-73 -0.9646891754474964 1102 74-75 -1.028828759365588 1102 76-77 -0.8295649342983324 1102 78-79 -0.8100082000310209 1102 80-81 -0.7764563590435536 1102 82-83 -0.7568554582357407 1102 84-85 -0.6911985313568003 1102 86-87 -0.7088303811343906 1102 88-89 -0.7907768533747337 1102 90-91 -0.7774839945274614 1102 92-93 -0.9063546433646685 1102 94-95 -0.9685261219323316 1102 96-97 -0.9378220694548176 1102 98-99 -1.0124141293225613 1102 100 -1.0086827157010525 1103 1 -0.33707190431931977 1103 2 -0.29375080912806695 1103 3 -0.33460856430640007 1103 4 -0.20237756302120147 1103 5 -0.256893785818022 1103 6 -0.33203302835876514 1103 7 -0.3860580294351692 1103 8 -0.3474733307186568 1103 9 -0.5957459389622315 1103 10-11 -0.5357094963425624 1103 12-13 -0.4820904324131803 1103 14-15 -0.6746537318799639 1103 16-17 -0.69796043179214 1103 18-19 -0.66848332155012 1103 20-21 -0.7568498952565363 1103 22-23 -0.7369051931621087 1103 24-25 -0.8131708444828618 1103 26-27 -0.7923315348360731 1103 28-29 -0.7676911699515898 1103 30-31 -0.7129025526594717 1103 32-33 -0.652291009109625 1103 34-35 -0.7766380897966201 1103 36-37 -0.7598607104902371 1103 38-39 -0.7661981377559499 1103 40-41 -0.7827677310955181 1103 42-43 -0.7797377229743816 1103 44-45 -0.772618410575113 1103 46-47 -0.7217386956134106 1103 48-49 -0.7958509629335211 1103 50-51 -0.872949903943173 1103 52-53 -0.8179914481925508 1103 54-55 -0.8147331926138293 1103 56-57 -0.7911108919060084 1103 58-59 -0.8423056648248348 1103 60-61 -0.927482808561706 1103 62-63 -0.8833921456696174 1103 64-65 -0.817466471698431 1103 66-67 -0.9113077813451937 1103 68-69 -0.8178753946058634 1103 70-71 -0.9919846060672555 1103 72-73 -0.9124037463181267 1103 74-75 -0.9175889030744813 1103 76-77 -0.8526198522565274 1103 78-79 -0.7530515340159276 1103 80-81 -0.6679858414721203 1103 82-83 -0.7923727011318817 1103 84-85 -0.8531741665233632 1103 86-87 -0.7198699509809217 1103 88-89 -0.7212430811508064 1103 90-91 -0.6712160573764621 1103 92-93 -0.8921646541059935 1103 94-95 -0.9241980246868877 1103 96-97 -1.0255843962072682 1103 98-99 -1.0956447093624924 1103 100 -1.2117686548441604 1104 1 -0.33287094522658833 1104 2 -0.30074908230355035 1104 3 -0.3550945429393728 1104 4 -0.2642425716348953 1104 5 -0.29635008157752196 1104 6 -0.44604879796623464 1104 7 -0.3706982865841866 1104 8 -0.3704569707944714 1104 9 -0.5424354497306894 1104 10-11 -0.5464714216562285 1104 12-13 -0.5115765279655022 1104 14-15 -0.6601899337390051 1104 16-17 -0.6273544252701271 1104 18-19 -0.7004655123571837 1104 20-21 -0.7516069103662346 1104 22-23 -0.7450984167837404 1104 24-25 -0.7242612062498424 1104 26-27 -0.7512276262230202 1104 28-29 -0.8682431865651665 1104 30-31 -0.7866158593408272 1104 32-33 -0.7717402086751548 1104 34-35 -0.8305318649275577 1104 36-37 -0.6451554946685008 1104 38-39 -0.6950884485469118 1104 40-41 -0.7559380097210706 1104 42-43 -0.6706867696322902 1104 44-45 -0.8272236096250225 1104 46-47 -0.9554816972210745 1104 48-49 -0.834499990872068 1104 50-51 -0.7969057688988173 1104 52-53 -0.8042519099034422 1104 54-55 -0.803187356110385 1104 56-57 -0.9107880229109 1104 58-59 -0.9639693011445232 1104 60-61 -0.9366841806514259 1104 62-63 -0.9395018045763166 1104 64-65 -0.9296073609405369 1104 66-67 -1.0017507347483061 1104 68-69 -0.8960099821880192 1104 70-71 -1.0930216132533985 1104 72-73 -0.9707353154200149 1104 74-75 -0.8781153962697061 1104 76-77 -0.8856987833018266 1104 78-79 -0.7486612704489417 1104 80-81 -0.779063867805462 1104 82-83 -0.8118249956212793 1104 84-85 -0.7851954745805081 1104 86-87 -0.8715013027163323 1104 88-89 -0.898938850066429 1104 90-91 -0.9066868198111244 1104 92-93 -0.8951822048778943 1104 94-95 -1.012745924541008 1104 96-97 -0.9029508638613635 1104 98-99 -0.9468637518672729 1104 100 -0.9009751401975414 1105 1 -0.36890477142230793 1105 2 -0.353037507941913 1105 3 -0.37833244176880143 1105 4 -0.24886551678088153 1105 5 -0.2513414139038517 1105 6 -0.3142851312087913 1105 7 -0.31653047675013113 1105 8 -0.3990107255001547 1105 9 -0.5061951904518267 1105 10-11 -0.5228925614646656 1105 12-13 -0.5348608844378973 1105 14-15 -0.7728254096995144 1105 16-17 -0.6786625657438989 1105 18-19 -0.6851662948786341 1105 20-21 -0.780510295807602 1105 22-23 -0.7816273322043159 1105 24-25 -0.7271066646370272 1105 26-27 -0.8023451594700646 1105 28-29 -0.7783381281046999 1105 30-31 -0.7978292520610708 1105 32-33 -0.6614442736594199 1105 34-35 -0.7494017083926678 1105 36-37 -0.6113973790299383 1105 38-39 -0.6688484136518369 1105 40-41 -0.7946080734222676 1105 42-43 -0.7567559362743026 1105 44-45 -0.8162950823204085 1105 46-47 -0.7232817229912101 1105 48-49 -0.8387303731846458 1105 50-51 -0.7986291553669034 1105 52-53 -0.9833109700957507 1105 54-55 -0.8516239960787786 1105 56-57 -0.9069184905233172 1105 58-59 -0.9212073271247618 1105 60-61 -0.8860259202173282 1105 62-63 -0.9484277745857312 1105 64-65 -0.891165641739164 1105 66-67 -0.9278415204281387 1105 68-69 -0.9465404118591678 1105 70-71 -0.9855623822574344 1105 72-73 -1.0081620719625874 1105 74-75 -1.028172070174449 1105 76-77 -0.9520162102045617 1105 78-79 -0.8848223834469167 1105 80-81 -0.8243056736325727 1105 82-83 -0.8400434325570245 1105 84-85 -0.8797136766952214 1105 86-87 -0.8010694761201194 1105 88-89 -0.8173795085444446 1105 90-91 -0.8428749608560544 1105 92-93 -0.903627823597489 1105 94-95 -0.9066947527986784 1105 96-97 -1.03990542852452 1105 98-99 -0.9573485942325632 1105 100 -0.9111849084396901 1106 1 -0.4342296726901864 1106 2 -0.4165552682579907 1106 3 -0.46095562212509833 1106 4 -0.31161237237652983 1106 5 -0.35960462896772327 1106 6 -0.3727800055017525 1106 7 -0.43707717836717563 1106 8 -0.41854820290161854 1106 9 -0.604412060180735 1106 10-11 -0.5627111054664269 1106 12-13 -0.7039279278564265 1106 14-15 -0.7924214299966295 1106 16-17 -0.6883503527044113 1106 18-19 -0.7218493036462448 1106 20-21 -0.8500370493376295 1106 22-23 -0.6707237853344168 1106 24-25 -0.7165500417527539 1106 26-27 -0.8051555725515698 1106 28-29 -0.7943679553053613 1106 30-31 -0.7974217618078185 1106 32-33 -0.7922344476255674 1106 34-35 -0.7628155854195384 1106 36-37 -0.7689511998812577 1106 38-39 -0.7462265248813083 1106 40-41 -0.8074931253234396 1106 42-43 -0.7037020440991242 1106 44-45 -0.7786291363113875 1106 46-47 -0.714786713806788 1106 48-49 -0.8531247762679968 1106 50-51 -0.8678982999223379 1106 52-53 -0.8142612602304311 1106 54-55 -0.8692992462265678 1106 56-57 -0.9103991624311902 1106 58-59 -0.935747547881391 1106 60-61 -0.9363288695854806 1106 62-63 -0.9690070693529194 1106 64-65 -0.9446499051322874 1106 66-67 -0.848136093036338 1106 68-69 -0.8355541765214554 1106 70-71 -0.8505454516924758 1106 72-73 -0.8352516334877649 1106 74-75 -0.9056909069244128 1106 76-77 -0.8798627489064401 1106 78-79 -0.6703745802049994 1106 80-81 -0.744451444093837 1106 82-83 -0.7259846225762701 1106 84-85 -0.7357615860967179 1106 86-87 -0.7283321877015965 1106 88-89 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1107 46-47 -1.0107368483038996 1107 48-49 -1.0123485593567167 1107 50-51 -1.0801468697363035 1107 52-53 -1.0911952102243134 1107 54-55 -0.9927712147387311 1107 56-57 -1.0708114364401595 1107 58-59 -1.0955757171377485 1107 60-61 -1.2057696446877344 1107 62-63 -1.0668195192264918 1107 64-65 -1.0024015806739683 1107 66-67 -1.000611243521142 1107 68-69 -1.044507791375338 1107 70-71 -1.0360884105655899 1107 72-73 -0.9664162932507026 1107 74-75 -1.054395659911954 1107 76-77 -1.0603902622453418 1107 78-79 -0.9799056118912333 1107 80-81 -0.8228698815423918 1107 82-83 -0.8428799382883199 1107 84-85 -0.9468904898898636 1107 86-87 -0.9443606856290039 1107 88-89 -0.8439337460606744 1107 90-91 -0.9193625542666517 1107 92-93 -1.0657822340816416 1107 94-95 -0.9988209520957234 1107 96-97 -1.0096365861034435 1107 98-99 -1.0893491938810875 1107 100 -1.1152824077272356 1108 1 -0.5351537748368003 1108 2 -0.4763924953583434 1108 3 -0.4469002569118281 1108 4 -0.31025313462681225 1108 5 -0.3618435954136672 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0.7743421534036585 2310 66-67 1.1968445732374278 2310 68-69 1.2791922770725677 2310 70-71 1.270785038106446 2310 72-73 1.3820019416459743 2310 74-75 1.3277856240105663 2310 76-77 1.3110344839225263 2310 78-79 1.138930106783782 2310 80-81 1.076289656120963 2310 82-83 1.057788847162918 2310 84-85 1.112219317538763 2310 86-87 1.0797623699897088 2310 88-89 1.1511891253580266 2310 90-91 1.2769359433028988 2310 92-93 1.2412955597943487 2310 94-95 1.2771613690095727 2310 96-97 1.3334963411716778 2310 98-99 1.3072779031569013 2310 100 1.407492265600343 2311 1 0.37168292371084277 2311 2 0.30714474027650596 2311 3 0.38191301859669835 2311 4 0.30464623324175477 2311 5 0.3701940430989552 2311 6 0.40754730971775643 2311 7 0.4858684323149163 2311 8 0.4837781911956114 2311 9 0.6148555053667906 2311 10-11 0.6245832368514002 2311 12-13 0.6610998836139359 2311 14-15 0.7805021601733486 2311 16-17 0.6811478867322123 2311 18-19 0.7829326280898457 2311 20-21 0.6968750735711993 2311 22-23 0.8758788273424969 2311 24-25 0.903125999714554 2311 26-27 0.306464447482945 2311 28-29 0.6406525145575159 2311 30-31 0.8377359065674739 2311 32-33 0.9203651376754465 2311 34-35 0.9986533459924161 2311 36-37 0.9453446167925605 2311 38-39 1.0155652249751554 2311 40-41 0.9687719253490599 2311 42-43 0.9823993004721601 2311 44-45 0.9927579156490509 2311 46-47 0.6858581310550136 2311 48-49 0.9308821250370087 2311 50-51 1.1039218023537032 2311 52-53 1.0101472193531578 2311 54-55 1.0755100605567236 2311 56-57 1.1533340832295806 2311 58-59 1.143263929940126 2311 60-61 1.1704205062755193 2311 62-63 1.2359758810487236 2311 64-65 1.283873360640591 2311 66-67 1.2472411727003347 2311 68-69 1.259585265529303 2311 70-71 1.2735810284824893 2311 72-73 1.256716143611854 2311 74-75 1.268840994208169 2311 76-77 1.2412592541056355 2311 78-79 1.006423461688172 2311 80-81 0.9437972910332917 2311 82-83 1.0118090794387342 2311 84-85 1.0877731312045178 2311 86-87 1.0709949178192701 2311 88-89 1.0648710944767963 2311 90-91 1.1602561421069915 2311 92-93 1.2089579816379619 2311 94-95 1.2258355356815152 2311 96-97 1.2535714335662966 2311 98-99 1.3434749420263117 2311 100 1.4805848215102593 2312 1 0.2260461312580091 2312 2 0.3209261314537599 2312 3 0.4538815831285419 2312 4 0.19744287156224516 2312 5 0.33793227279157634 2312 6 0.4049113089648131 2312 7 0.34105586587855896 2312 8 0.4332305065025466 2312 9 0.5907287281943283 2312 10-11 0.6148510030397247 2312 12-13 0.6578455403372985 2312 14-15 0.8637486418147731 2312 16-17 0.8162929255974731 2312 18-19 0.8222605274830599 2312 20-21 0.7841897438254364 2312 22-23 0.8918955250128064 2312 24-25 0.8467350300759691 2312 26-27 0.959288476958541 2312 28-29 0.949829658790847 2312 30-31 0.9341132567534913 2312 32-33 0.8074056887650016 2312 34-35 0.9436122108165748 2312 36-37 1.0142619533591315 2312 38-39 0.7791618400429172 2312 40-41 0.9440714425782204 2312 42-43 1.040675401654731 2312 44-45 0.7917363875021763 2312 46-47 0.8551756774497363 2312 48-49 1.0609925339527635 2312 50-51 0.826733416535653 2312 52-53 1.0602149705933002 2312 54-55 0.9065786754694685 2312 56-57 0.953085223720322 2312 58-59 1.0935906445516963 2312 60-61 1.1697869387540294 2312 62-63 1.2456745506607305 2312 64-65 1.24074405611848 2312 66-67 1.1608140506124585 2312 68-69 1.256534383586711 2312 70-71 1.1527263801522594 2312 72-73 1.1747627487141585 2312 74-75 1.204862093286927 2312 76-77 1.1654716372883769 2312 78-79 1.0723539305073757 2312 80-81 1.0181226160177914 2312 82-83 1.0120305339519788 2312 84-85 1.1031977840745704 2312 86-87 1.0693488879450541 2312 88-89 1.0348013778278577 2312 90-91 1.144607266542291 2312 92-93 1.1454119550855744 2312 94-95 1.1280436447921538 2312 96-97 0.7617296102205628 2312 98-99 0.5628827311351792 2312 100 0.7882854869810707 2313 1 0.08798158727853433 2313 2 0.24187781186115842 2313 3 0.2790115330808476 2313 4 0.1934381655444568 2313 5 0.3618901036380606 2313 6 0.255691642497311 2313 7 0.24845093634549187 2313 8 0.3894573635569145 2313 9 0.6166439451864179 2313 10-11 0.3726294138748045 2313 12-13 0.40825482444427763 2313 14-15 0.4942468900770862 2313 16-17 0.7431574915721697 2313 18-19 0.7190052606214223 2313 20-21 0.6789834718302075 2313 22-23 0.6606664924187626 2313 24-25 0.5679367519385394 2313 26-27 0.7339522875584592 2313 28-29 0.4734117807882541 2313 30-31 0.7490064788682105 2313 32-33 0.8725983473635566 2313 34-35 0.9233480009451753 2313 36-37 0.9339486516642026 2313 38-39 0.9295946696560264 2313 40-41 0.9064382036210503 2313 42-43 0.975292854416395 2313 44-45 0.872441072209952 2313 46-47 0.8783506137937849 2313 48-49 0.9338623465754168 2313 50-51 0.8501191832300918 2313 52-53 0.8499621232058701 2313 54-55 0.8034029785430405 2313 56-57 0.4792220202844035 2313 58-59 0.5685203335077631 2313 60-61 0.4476678928806379 2313 62-63 0.7668258925061977 2313 64-65 0.8929490512260898 2313 66-67 0.8801938008262411 2313 68-69 0.9137534837286836 2313 70-71 0.8780156755377888 2313 72-73 0.9743316492546512 2313 74-75 1.0263231754517435 2313 76-77 0.9479420379576879 2313 78-79 0.8896921080423823 2313 80-81 0.7649079992005596 2313 82-83 0.8166819748101872 2313 84-85 0.8294495844621217 2313 86-87 0.8516245178580135 2313 88-89 0.8313382698416056 2313 90-91 0.7884020877985769 2313 92-93 0.9577347856442593 2313 94-95 1.0916683217159466 2313 96-97 1.2000348596778778 2313 98-99 1.1478154144560868 2313 100 1.240635258258191 2314 1 -0.39017774020139484 2314 2 0.007911986222623568 2314 3 0.31546546293456856 2314 4 0.20243849328441144 2314 5 0.29763813014103846 2314 6 0.3866271898151723 2314 7 0.4440040084874326 2314 8 0.49879032375732635 2314 9 0.4398939698766071 2314 10-11 0.5697533343466645 2314 12-13 0.46765052006549723 2314 14-15 0.7542205553977155 2314 16-17 0.8345517701051008 2314 18-19 0.8427012235754674 2314 20-21 0.8764053094935491 2314 22-23 0.9098026981305125 2314 24-25 0.826521144231755 2314 26-27 0.788070544147466 2314 28-29 0.8272690975144457 2314 30-31 0.8093809033468347 2314 32-33 0.929395675260551 2314 34-35 0.8978689099512707 2314 36-37 0.8262030551058714 2314 38-39 0.5646636291557598 2314 40-41 0.5979383809019652 2314 42-43 0.8135782909988833 2314 44-45 0.9003511426512745 2314 46-47 0.8266901688605088 2314 48-49 0.8746360881231183 2314 50-51 0.8496990672156173 2314 52-53 0.9226997386050613 2314 54-55 0.8196518312669383 2314 56-57 0.8308883883785185 2314 58-59 0.9466042576187874 2314 60-61 1.0255593204733273 2314 62-63 0.9357014373355881 2314 64-65 0.9947412192263059 2314 66-67 0.734894257352515 2314 68-69 0.7044715926815641 2314 70-71 0.8328118691349928 2314 72-73 0.9302152250788538 2314 74-75 0.9643512670457497 2314 76-77 0.8171473261026989 2314 78-79 0.7773698349460005 2314 80-81 0.8020335517495027 2314 82-83 0.7894017006907745 2314 84-85 0.8211548827481145 2314 86-87 0.9234393246673562 2314 88-89 0.9246960803300901 2314 90-91 0.9711817559840163 2314 92-93 0.8627572121136602 2314 94-95 0.8191238340594253 2314 96-97 0.8925978738721874 2314 98-99 1.0440894544797672 2314 100 0.9722209749221591 2315 1 0.3741121892152748 2315 2 0.3791397821194451 2315 3 0.41118601175235625 2315 4 0.29111872638868874 2315 5 0.3399587109383262 2315 6 0.36241226442648156 2315 7 0.3034517508888328 2315 8 0.23775530840012493 2315 9 0.46740731594446316 2315 10-11 0.5265028580845623 2315 12-13 0.35234246449217466 2315 14-15 0.526396380285874 2315 16-17 0.6687516764467745 2315 18-19 0.6942122550993091 2315 20-21 0.48919352354415935 2315 22-23 0.7884001530197295 2315 24-25 0.826279282443231 2315 26-27 0.7567493712774009 2315 28-29 0.6817253663582363 2315 30-31 0.7876101664163571 2315 32-33 0.7131705644774655 2315 34-35 0.724988678891016 2315 36-37 0.7268160709102389 2315 38-39 0.815853800886174 2315 40-41 0.8286264805405494 2315 42-43 0.8597222730317284 2315 44-45 0.8883257053059168 2315 46-47 0.8401238912587985 2315 48-49 0.7142632671339442 2315 50-51 0.7906321736603275 2315 52-53 0.9099799767338652 2315 54-55 0.7247024815636607 2315 56-57 0.687356588989104 2315 58-59 0.7774317698125515 2315 60-61 0.8453064478658021 2315 62-63 0.8786334663145325 2315 64-65 0.9209180285000826 2315 66-67 0.9652924485846555 2315 68-69 1.0086469944337395 2315 70-71 0.8719429892103747 2315 72-73 0.9789621122789569 2315 74-75 0.39313981717838686 2315 76-77 0.38120129572853045 2315 78-79 0.6439557982242974 2315 80-81 0.6867926698269642 2315 82-83 0.6634040564408821 2315 84-85 0.7790704938312594 2315 86-87 0.7220306304038218 2315 88-89 0.7554644707533207 2315 90-91 0.8564750567667154 2315 92-93 0.8377604807087913 2315 94-95 0.8736120172584094 2315 96-97 1.0164420625991397 2315 98-99 1.0170193282828812 2315 100 0.9286667768509531 2316 1 0.2566928980625107 2316 2 0.05511612873836924 2316 3 0.15297752308347867 2316 4 -0.061572524158734154 2316 5 0.22256694871924765 2316 6 0.31798427128004647 2316 7 0.34358478242469204 2316 8 0.3978069813489 2316 9 0.5545755261182634 2316 10-11 0.5546413988279184 2316 12-13 0.2763911341808907 2316 14-15 0.41063933175895784 2316 16-17 0.6774233720034815 2316 18-19 0.6754603477229253 2316 20-21 0.6448293071182007 2316 22-23 0.6625400469160496 2316 24-25 0.3476186448845269 2316 26-27 0.6236214027033995 2316 28-29 0.7951950089080668 2316 30-31 0.46831336533546875 2316 32-33 0.5877561949767909 2316 34-35 0.7167915723774172 2316 36-37 0.805609682177753 2316 38-39 0.7016876681695123 2316 40-41 0.7378734819433035 2316 42-43 0.6979668400785641 2316 44-45 0.5320251102004079 2316 46-47 0.7365343056335973 2316 48-49 0.801838323183425 2316 50-51 0.8392100517512944 2316 52-53 0.7982713103893531 2316 54-55 0.7156237161532601 2316 56-57 0.8802890076559677 2316 58-59 0.6783731168130487 2316 60-61 0.7610055227388841 2316 62-63 0.779098269554737 2316 64-65 0.8651688631846852 2316 66-67 0.8286655566118597 2316 68-69 0.8282561430314246 2316 70-71 0.7818024890259352 2316 72-73 0.8132685350479534 2316 74-75 0.855055186720513 2316 76-77 0.7367571028634217 2316 78-79 0.43286705007324144 2316 80-81 0.4695656413986171 2316 82-83 0.4316024937910967 2316 84-85 0.5045030992143431 2316 86-87 0.643886351751064 2316 88-89 0.6277298926778094 2316 90-91 0.4430722859062364 2316 92-93 0.2802453913102063 2316 94-95 0.3530658627613086 2316 96-97 0.6003640372935344 2316 98-99 0.7782123443064286 2316 100 0.5097992275914685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 3.0 12 30.0 13 112.0 14 445.0 15 1484.0 16 3624.0 17 7463.0 18 12533.0 19 18116.0 20 25075.0 21 34390.0 22 43162.0 23 45297.0 24 46142.0 25 48828.0 26 57170.0 27 69769.0 28 86469.0 29 107287.0 30 132718.0 31 165777.0 32 210377.0 33 277826.0 34 398934.0 35 553529.0 36 860562.0 37 1318872.0 38 1142951.0 39 175773.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73052747110543 20.070481480809477 13.521948093340377 23.677042954744714 2 15.544630261884821 20.160913778103236 40.91097216388397 23.383483796127972 3 18.97273533746981 25.812434930865248 29.60414868398878 25.61068104767616 4 12.71494679561877 16.43050704028635 38.097530867578534 32.75701529651635 5 13.19676050879893 37.608706624497735 34.313284839629446 14.881248027073893 6 30.75910069643757 36.76109051806366 18.177388406166564 14.302420379332204 7 27.637723752281772 32.25127975158302 22.170520963561962 17.940475532573245 8 26.25070897019459 35.86825430365225 19.791316815361494 18.08971991079166 9 26.60352408434586 16.163266786764424 20.90717383329044 36.326035295599276 10-11 25.732652217623965 26.246927998653145 27.44201437056737 20.578405413155522 12-13 25.62687727410497 23.783571719858756 28.079097383983164 22.510453622053113 14-15 22.93223801173603 25.189328450535925 26.013197856464714 25.865235681263332 16-17 22.1393839863624 27.91129847095445 26.926324756624542 23.02299278605861 18-19 21.76948881170084 27.31636943240205 29.187588952776295 21.72655280312082 20-21 22.767751202148254 26.647920482416254 28.864843547494374 21.719484767941115 22-23 22.761720143479312 26.360106386163984 28.687504758548066 22.19066871180864 24-25 22.213888951386043 26.307761522148958 29.211446828718035 22.266902697746964 26-27 22.20814114068566 26.558682051830868 28.73231855444267 22.500858253040796 28-29 21.901781038222936 27.131716961772618 28.805453521689685 22.161048478314765 30-31 22.7787674380028 26.614086493708676 28.79758121965093 21.809564848637592 32-33 22.759120711424174 26.6724516325488 28.441745972519815 22.12668168350721 34-35 22.707617549429735 26.746024982013417 28.73655455770801 21.809802910848834 36-37 22.500342720656054 26.62042690414484 28.63138848845386 22.247841886745242 38-39 22.53528514331822 26.778298492418457 28.72613252582158 21.960283838441747 40-41 22.452745625266644 26.193033465274045 29.089963240870464 22.264257668588844 42-43 22.366452571029676 26.474368406464617 28.517376307585966 22.64180271491974 44-45 22.16848031004348 26.98148477857991 28.613032308658838 22.237002602717773 46-47 22.220651668813296 26.65827409319008 28.181617731426297 22.939456506570327 48-49 22.609190352896995 26.729676908540046 28.161197970246654 22.499934768316308 50-51 22.497424753169863 26.81845793193135 28.02095275577078 22.66316455912801 52-53 22.263643932276544 27.135873409215684 27.749142488467626 22.851340170040146 54-55 22.189446716998617 26.919351951888853 27.96043006542971 22.93077126568282 56-57 22.60967550364697 26.842565057447587 27.648035773832753 22.899723665072692 58-59 22.061269230851494 27.065591827917434 28.1290063159966 22.74413262523447 60-61 22.47695010038508 27.324204627466465 27.69690583992387 22.501939432224585 62-63 22.583726547956157 27.0578056078991 27.526591979200184 22.83187586494456 64-65 22.10446304834765 26.94050324332179 27.687060310774907 23.267973397555654 66-67 22.194413642719045 26.773652943107002 27.660365994689716 23.371567419484233 68-69 21.904707936628316 26.919183156102022 27.777355964910917 23.398752942358744 70-71 21.986784861906788 26.690631422646472 27.875368432873405 23.447215282573335 72-73 22.182149450090854 27.005163718894377 27.68174586471558 23.13094096629919 74-75 22.350223513274727 27.198445351269623 27.359032938384004 23.09229819707165 76-77 22.21592203405901 26.973695582932944 27.32867591020081 23.481706472807236 78-79 21.988509598321922 26.98710298752497 27.503878446923714 23.520508967229393 80-81 21.817031120795896 27.269907301977508 27.51492511904422 23.39813645818238 82-83 22.382943638978357 26.892342277424408 27.90536046399776 22.819353619599475 84-85 22.4871849365433 27.142333930566487 27.21831403930441 23.152167093585803 86-87 21.7579521572908 27.454980973439508 27.51588342884847 23.27118344042122 88-89 22.04744456592245 26.84901893456 27.49822855277577 23.60530794674178 90-91 22.211013780449164 26.72251335070347 27.598600574785937 23.46787229406143 92-93 22.234764708706553 27.010356851237198 27.386625740314557 23.36825269974169 94-95 21.619363809626805 26.945388531844937 27.654614822037193 23.78063283649106 96-97 21.6894069714958 26.91978259374302 27.50383178014633 23.88697865461485 98-99 21.369003629098952 27.174252795196818 27.36331025711387 24.09343331859036 100 21.53716054318567 26.989863190380607 27.238285881718284 24.234690384715442 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1495.0 1 1103.5 2 1663.0 3 2418.5 4 1610.0 5 1078.0 6 1563.0 7 2947.5 8 4631.5 9 5327.0 10 4615.5 11 3842.0 12 3962.5 13 4389.0 14 5004.0 15 5769.0 16 6502.5 17 7222.5 18 7962.5 19 8686.0 20 9457.0 21 10449.0 22 11837.0 23 13796.5 24 16306.5 25 19281.0 26 23422.5 27 28579.0 28 34783.0 29 43789.5 30 55704.0 31 67776.0 32 83202.0 33 99654.5 34 113457.5 35 127264.0 36 142313.0 37 162540.0 38 173817.5 39 173556.5 40 171098.0 41 168234.0 42 171175.0 43 178968.5 44 189862.5 45 209897.5 46 227740.5 47 238213.5 48 247507.0 49 250121.5 50 248186.0 51 248012.0 52 243242.5 53 230561.0 54 217831.0 55 196899.5 56 173418.0 57 148971.0 58 125188.5 59 109984.5 60 89526.5 61 67561.5 62 52537.0 63 41635.0 64 30565.5 65 20139.0 66 13980.5 67 10253.5 68 7900.5 69 6011.0 70 4640.5 71 3600.0 72 2684.0 73 2069.5 74 1607.0 75 1084.0 76 708.0 77 433.0 78 246.5 79 146.0 80 92.5 81 57.5 82 39.0 83 26.5 84 20.5 85 15.5 86 8.5 87 4.5 88 3.5 89 3.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04522010797733691 2 0.0 3 0.0 4 0.040532136132542994 5 0.0 6 0.0 7 9.581256325126245E-4 8 0.0 9 0.010556491343933739 10-11 3.592971121922342E-4 12-13 8.554693147434149E-6 14-15 1.3687509035894638E-4 16-17 3.507424190448001E-4 18-19 8.554693147434149E-6 20-21 0.0 22-23 0.0 24-25 5.902738271729562E-4 26-27 4.0207057792940494E-4 28-29 1.3687509035894638E-4 30-31 0.002506525092198205 32-33 0.014739736293029038 34-35 0.025681188828597313 36-37 0.030694239012993726 38-39 0.02190001445743142 40-41 0.001274649278967688 42-43 8.554693147434149E-5 44-45 0.011317859034055378 46-47 0.018589348209374405 48-49 0.003293556861762147 50-51 0.01103555416019005 52-53 0.011198093329991301 54-55 0.010453835026164529 56-57 4.448440436665757E-4 58-59 0.007827544229902245 60-61 0.004294455960011942 62-63 0.0013430868241471612 64-65 0.03634033649030026 66-67 0.06197019716001297 68-69 0.06083242297140423 70-71 0.05322730076333527 72-73 0.046785616823317354 74-75 0.03659697728472328 76-77 0.032268302552121604 78-79 0.015586650914625017 80-81 0.0011634382680510442 82-83 0.03940291663708169 84-85 0.05277390202652126 86-87 0.05880496069546234 88-89 0.07154289879199179 90-91 0.04992518920842569 92-93 0.040728894074933984 94-95 0.013191336833343458 96-97 0.003473205417858264 98-99 0.021959897309463457 100 0.03218275562064726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5844745.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.5676522444401 #Duplication Level Percentage of deduplicated Percentage of total 1 78.83660531663682 51.6913112153342 2 13.22094206250969 17.33732262997052 3 3.9121167413257316 7.6952493010469345 4 1.5026003243696648 3.940879021226123 5 0.7635377375760629 2.503168842644693 6 0.44941407047233023 1.7680215291892825 7 0.2910907978218317 1.336029814219694 8 0.20052532186772098 1.051837965634172 9 0.14083939458127165 0.831105760160007 >10 0.6368214722279514 7.054113903676407 >50 0.029673093925601135 1.3388124985718521 >100 0.014596628134392386 1.7401527610274803 >500 8.246931810813203E-4 0.3518291405789116 >1k 3.171887460618036E-4 0.4482030734390052 >5k 0.0 0.0 >10k+ 9.515662381798392E-5 0.9119625432807513 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18552 0.3174133345423966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15233 0.2606272814297288 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10065 0.1722059730578494 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 1.7109386294868297E-5 0.0 0.0 1.7109386294868297E-5 0.0 10-11 1.7109386294868297E-5 8.554693147434149E-6 0.0 1.7109386294868297E-5 0.0 12-13 2.5664079442302444E-5 1.7109386294868297E-5 0.0 1.7109386294868297E-5 0.0 14-15 3.4218772589736595E-5 2.5664079442302444E-5 0.0 1.7109386294868297E-5 1.7109386294868297E-5 16-17 3.4218772589736595E-5 3.4218772589736595E-5 0.0 1.7109386294868297E-5 3.4218772589736595E-5 18-19 3.4218772589736595E-5 4.2773465737170745E-5 0.0 4.2773465737170745E-5 5.132815888460489E-5 20-21 3.4218772589736595E-5 5.132815888460489E-5 0.0 1.0265631776920978E-4 5.132815888460489E-5 22-23 3.4218772589736595E-5 5.132815888460489E-5 0.0 4.6195342996144397E-4 5.132815888460489E-5 24-25 3.4218772589736595E-5 5.132815888460489E-5 0.0 0.0010522272571344001 5.132815888460489E-5 26-27 3.4218772589736595E-5 5.132815888460489E-5 0.0 0.0019932435033521564 7.699223832690733E-5 28-29 3.4218772589736595E-5 5.132815888460489E-5 0.0 0.0051413705816079226 8.554693147434148E-5 30-31 3.4218772589736595E-5 5.132815888460489E-5 0.0 0.01689551896618244 8.554693147434148E-5 32-33 3.4218772589736595E-5 5.132815888460489E-5 0.0 0.04553663162379197 8.554693147434148E-5 34-35 3.4218772589736595E-5 5.132815888460489E-5 0.0 0.08746318273936673 1.0265631776920978E-4 36-37 3.4218772589736595E-5 5.988285203203904E-5 0.0 0.15478861780967346 1.1121101091664392E-4 38-39 3.4218772589736595E-5 6.843754517947319E-5 0.0 0.25784700615681266 1.1976570406407808E-4 40-41 3.4218772589736595E-5 7.699223832690733E-5 0.0 0.37618407646526925 1.1976570406407808E-4 42-43 4.2773465737170745E-5 8.554693147434148E-5 0.0 0.48615465687553516 1.1976570406407808E-4 44-45 5.132815888460489E-5 8.554693147434148E-5 0.0 0.6098982932531702 1.1976570406407808E-4 46-47 5.132815888460489E-5 1.0265631776920978E-4 0.0 0.7564915150276017 1.3687509035894638E-4 48-49 5.132815888460489E-5 1.1976570406407808E-4 0.0 0.9153778308548961 1.8820324924355127E-4 50-51 5.132815888460489E-5 1.1976570406407808E-4 0.0 1.081877823583407 1.8820324924355127E-4 52-53 5.132815888460489E-5 1.1976570406407808E-4 0.0 1.2442801182942969 2.0531263553841955E-4 54-55 5.132815888460489E-5 1.1976570406407808E-4 0.0 1.4291384825172013 2.2242202183328784E-4 56-57 5.132815888460489E-5 1.1976570406407808E-4 0.0 1.6341517037954607 2.2242202183328784E-4 58-59 5.132815888460489E-5 1.1976570406407808E-4 0.0 1.8659753333977787 2.30976714980722E-4 60-61 5.132815888460489E-5 1.1976570406407808E-4 0.0 2.1027949037981983 2.3953140812815616E-4 62-63 5.132815888460489E-5 1.1976570406407808E-4 0.0 2.3461588144564045 2.566407944230244E-4 64-65 5.132815888460489E-5 1.1976570406407808E-4 0.0 2.5944930018332704 2.566407944230244E-4 66-67 5.132815888460489E-5 1.1976570406407808E-4 0.0 2.86835439356208 2.566407944230244E-4 68-69 6.843754517947319E-5 1.1976570406407808E-4 0.0 3.170540374302044 2.566407944230244E-4 70-71 6.843754517947319E-5 1.1976570406407808E-4 0.0 3.483299955772236 2.566407944230244E-4 72-73 6.843754517947319E-5 1.1976570406407808E-4 0.0 3.786255858895469 3.250783396024976E-4 74-75 6.843754517947319E-5 1.1976570406407808E-4 0.0 4.0902126611169525 3.250783396024976E-4 76-77 6.843754517947319E-5 1.1976570406407808E-4 0.0 4.429722768059172 3.250783396024976E-4 78-79 6.843754517947319E-5 1.1976570406407808E-4 0.0 4.801432740008332 3.250783396024976E-4 80-81 6.843754517947319E-5 1.1976570406407808E-4 0.0 5.1873777213548244 3.250783396024976E-4 82-83 6.843754517947319E-5 1.1976570406407808E-4 0.0 5.5757265714757445 3.421877258973659E-4 84-85 6.843754517947319E-5 1.1976570406407808E-4 0.0 5.96335682737228 3.421877258973659E-4 86-87 6.843754517947319E-5 1.1976570406407808E-4 0.0 6.381022610909458 3.421877258973659E-4 88 6.843754517947319E-5 1.1976570406407808E-4 0.0 6.7136376351748455 3.421877258973659E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9490 0.0 49.741524 1 TACCTGG 4820 0.0 41.43813 2 GTATCAA 16905 0.0 39.77154 1 ACCTGGG 5950 0.0 32.859196 3 CAACGCA 20280 0.0 32.17853 5 ATCAACG 20330 0.0 32.01791 3 GTACCTG 6425 0.0 31.7591 1 TATCAAC 21820 0.0 30.540756 2 AACGCAG 22330 0.0 29.771427 6 TCAACGC 22310 0.0 29.28167 4 GTACTAG 3080 0.0 28.85112 1 GTACATG 21675 0.0 26.767488 1 TACATGG 21610 0.0 26.466352 2 ACGCAGA 24945 0.0 26.198448 7 GTGGTAT 3815 0.0 25.880718 1 CTAACGC 1420 0.0 25.815876 3 CCTGGGG 5665 0.0 25.80127 4 CGCAGAG 25585 0.0 25.561245 8 ACATGGG 21655 0.0 25.349257 3 TCTAACG 1470 0.0 24.936502 2 >>END_MODULE