FastQCFastQC Report
Mon 6 Jun 2016
ERR1211257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6360619
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205730.3234433629808671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159710.2510919141674733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99940.15712307245568394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103300.047.970431
GTATCAA186700.041.84171
CAACGCA225750.033.3167575
ATCAACG226200.033.2917753
GTGGTAT40200.032.5435031
TATCAAC240300.032.1958922
GTACTAG42100.031.4101331
AACGCAG252800.030.3478036
TACCTGG43400.030.1444572
TCAACGC254100.029.9323584
ACGCAGA273450.027.5405227
CGCAGAG278050.027.0481038
GCATCGT43750.026.8597358
GTACATG252250.026.5472351
TACATGG252550.025.863932
TAGGCAT56900.025.0323055
TGGTATC50200.025.0299472
ACATGGG252400.024.8819373
CTGTGCG55900.024.552589
GCAGAGT298900.024.3585599
Produced by FastQC (version 0.11.5)